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( A ) Endogenous expression of Rab11a was examined in HBE and lung cancer cell lines by western blot and RT-qPCR. Lung cancer cell lines showed significant upregulated Rab11a expression. ( B ) Western blot and RT-qPCR analysis showed that <t>pCMV6-Rab11a</t> plasmid markedly increases its levels in H460 and H1299 cells compared with control. Rab11a plasmid downregulated its expression in A549 cells. ( C ) MTT showed that Rab11a overexpression in H1299 and H460 cells greatly promoted the proliferation rate while Rab11a depletion inhibited proliferation rate. ( D ) Rab11a overexpression in H1299 and H460 cells promoted the colony formation ability while Rab11a depletion inhibited colony formation ability. ( E ) Rab11a overexpression in H1299 and H460 cells greatly promoted cell invasion while Rab11a depletion inhibited invading ability of A549 cells. ( F ) Wound healing assay demonstrated that Rab11a overexpression increased cell migration in H1299 and H460 cell lines while its depletion downregulated A549 cell migration.
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( A ) Endogenous expression of Rab11a was examined in HBE and lung cancer cell lines by western blot and RT-qPCR. Lung cancer cell lines showed significant upregulated Rab11a expression. ( B ) Western blot and RT-qPCR analysis showed that <t>pCMV6-Rab11a</t> plasmid markedly increases its levels in H460 and H1299 cells compared with control. Rab11a plasmid downregulated its expression in A549 cells. ( C ) MTT showed that Rab11a overexpression in H1299 and H460 cells greatly promoted the proliferation rate while Rab11a depletion inhibited proliferation rate. ( D ) Rab11a overexpression in H1299 and H460 cells promoted the colony formation ability while Rab11a depletion inhibited colony formation ability. ( E ) Rab11a overexpression in H1299 and H460 cells greatly promoted cell invasion while Rab11a depletion inhibited invading ability of A549 cells. ( F ) Wound healing assay demonstrated that Rab11a overexpression increased cell migration in H1299 and H460 cell lines while its depletion downregulated A549 cell migration.
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( A ) Endogenous expression of Rab11a was examined in HBE and lung cancer cell lines by western blot and RT-qPCR. Lung cancer cell lines showed significant upregulated Rab11a expression. ( B ) Western blot and RT-qPCR analysis showed that <t>pCMV6-Rab11a</t> plasmid markedly increases its levels in H460 and H1299 cells compared with control. Rab11a plasmid downregulated its expression in A549 cells. ( C ) MTT showed that Rab11a overexpression in H1299 and H460 cells greatly promoted the proliferation rate while Rab11a depletion inhibited proliferation rate. ( D ) Rab11a overexpression in H1299 and H460 cells promoted the colony formation ability while Rab11a depletion inhibited colony formation ability. ( E ) Rab11a overexpression in H1299 and H460 cells greatly promoted cell invasion while Rab11a depletion inhibited invading ability of A549 cells. ( F ) Wound healing assay demonstrated that Rab11a overexpression increased cell migration in H1299 and H460 cell lines while its depletion downregulated A549 cell migration.
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( A ) Endogenous expression of Rab11a was examined in HBE and lung cancer cell lines by western blot and RT-qPCR. Lung cancer cell lines showed significant upregulated Rab11a expression. ( B ) Western blot and RT-qPCR analysis showed that <t>pCMV6-Rab11a</t> plasmid markedly increases its levels in H460 and H1299 cells compared with control. Rab11a plasmid downregulated its expression in A549 cells. ( C ) MTT showed that Rab11a overexpression in H1299 and H460 cells greatly promoted the proliferation rate while Rab11a depletion inhibited proliferation rate. ( D ) Rab11a overexpression in H1299 and H460 cells promoted the colony formation ability while Rab11a depletion inhibited colony formation ability. ( E ) Rab11a overexpression in H1299 and H460 cells greatly promoted cell invasion while Rab11a depletion inhibited invading ability of A549 cells. ( F ) Wound healing assay demonstrated that Rab11a overexpression increased cell migration in H1299 and H460 cell lines while its depletion downregulated A549 cell migration.
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( A ) Endogenous expression of Rab11a was examined in HBE and lung cancer cell lines by western blot and RT-qPCR. Lung cancer cell lines showed significant upregulated Rab11a expression. ( B ) Western blot and RT-qPCR analysis showed that pCMV6-Rab11a plasmid markedly increases its levels in H460 and H1299 cells compared with control. Rab11a plasmid downregulated its expression in A549 cells. ( C ) MTT showed that Rab11a overexpression in H1299 and H460 cells greatly promoted the proliferation rate while Rab11a depletion inhibited proliferation rate. ( D ) Rab11a overexpression in H1299 and H460 cells promoted the colony formation ability while Rab11a depletion inhibited colony formation ability. ( E ) Rab11a overexpression in H1299 and H460 cells greatly promoted cell invasion while Rab11a depletion inhibited invading ability of A549 cells. ( F ) Wound healing assay demonstrated that Rab11a overexpression increased cell migration in H1299 and H460 cell lines while its depletion downregulated A549 cell migration.

Journal: Oncotarget

Article Title: Rab11a promotes proliferation and invasion through regulation of YAP in non-small cell lung cancer

doi: 10.18632/oncotarget.15359

Figure Lengend Snippet: ( A ) Endogenous expression of Rab11a was examined in HBE and lung cancer cell lines by western blot and RT-qPCR. Lung cancer cell lines showed significant upregulated Rab11a expression. ( B ) Western blot and RT-qPCR analysis showed that pCMV6-Rab11a plasmid markedly increases its levels in H460 and H1299 cells compared with control. Rab11a plasmid downregulated its expression in A549 cells. ( C ) MTT showed that Rab11a overexpression in H1299 and H460 cells greatly promoted the proliferation rate while Rab11a depletion inhibited proliferation rate. ( D ) Rab11a overexpression in H1299 and H460 cells promoted the colony formation ability while Rab11a depletion inhibited colony formation ability. ( E ) Rab11a overexpression in H1299 and H460 cells greatly promoted cell invasion while Rab11a depletion inhibited invading ability of A549 cells. ( F ) Wound healing assay demonstrated that Rab11a overexpression increased cell migration in H1299 and H460 cell lines while its depletion downregulated A549 cell migration.

Article Snippet: RPMI-1640 (Gibco, USA) with 10% FBS was used for cell culture. pCMV6-Rab11a plasmid and the control empty vector pCMV6 were obtained from Origene (Origene, USA).

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Plasmid Preparation, Over Expression, Wound Healing Assay, Migration