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pcatenhancer  (Promega)

 
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    Structured Review

    Promega pcatenhancer
    Cloning of the human BRCA2 gene silencer. (A) Sketch showing the promoter and the silencer regions in the context of the upstream sequence of human BRCA2 gene. (B) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATbasic and <t>pCATenhancer</t> constructs. (C) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATcontrol constructs having the silencer sequence. Results are means ± SE (n = 6).
    Pcatenhancer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcatenhancer/product/Promega
    Average 90 stars, based on 1 article reviews
    pcatenhancer - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "Identification and Characterization of a Transcriptional Silencer Upstream of the Human BRCA2 Gene"

    Article Title: Identification and Characterization of a Transcriptional Silencer Upstream of the Human BRCA2 Gene

    Journal: Biochemical and biophysical research communications

    doi: 10.1006/bbrc.1999.1652

    Cloning of the human BRCA2 gene silencer. (A) Sketch showing the promoter and the silencer regions in the context of the upstream sequence of human BRCA2 gene. (B) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATbasic and pCATenhancer constructs. (C) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATcontrol constructs having the silencer sequence. Results are means ± SE (n = 6).
    Figure Legend Snippet: Cloning of the human BRCA2 gene silencer. (A) Sketch showing the promoter and the silencer regions in the context of the upstream sequence of human BRCA2 gene. (B) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATbasic and pCATenhancer constructs. (C) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATcontrol constructs having the silencer sequence. Results are means ± SE (n = 6).

    Techniques Used: Cloning, Sequencing, Activity Assay, Transfection, Construct



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    Cloning of the human BRCA2 gene silencer. (A) Sketch showing the promoter and the silencer regions in the context of the upstream sequence of human BRCA2 gene. (B) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATbasic and <t>pCATenhancer</t> constructs. (C) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATcontrol constructs having the silencer sequence. Results are means ± SE (n = 6).
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    Cloning of the human BRCA2 gene silencer. (A) Sketch showing the promoter and the silencer regions in the context of the upstream sequence of human BRCA2 gene. (B) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATbasic and <t>pCATenhancer</t> constructs. (C) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATcontrol constructs having the silencer sequence. Results are means ± SE (n = 6).
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    Image Search Results


    Cloning of the human BRCA2 gene silencer. (A) Sketch showing the promoter and the silencer regions in the context of the upstream sequence of human BRCA2 gene. (B) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATbasic and pCATenhancer constructs. (C) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATcontrol constructs having the silencer sequence. Results are means ± SE (n = 6).

    Journal: Biochemical and biophysical research communications

    Article Title: Identification and Characterization of a Transcriptional Silencer Upstream of the Human BRCA2 Gene

    doi: 10.1006/bbrc.1999.1652

    Figure Lengend Snippet: Cloning of the human BRCA2 gene silencer. (A) Sketch showing the promoter and the silencer regions in the context of the upstream sequence of human BRCA2 gene. (B) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATbasic and pCATenhancer constructs. (C) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATcontrol constructs having the silencer sequence. Results are means ± SE (n = 6).

    Article Snippet: Transformation of cells and reporter gene assay ( 4 – 6 ) The following plasmid vectors, procured from Promega (Madison, WI), were employed in our study: pCATbasic, pCATenhancer, pCATcontrol, pSV-b-galactosidase, pGL3-basic, pGL3-enhancer, pGL3-control and pRL-TK ( 5 ).

    Techniques: Cloning, Sequencing, Activity Assay, Transfection, Construct