Review




Structured Review

Eurofins pca7-flag
Pca7 Flag, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pca7-flag/pca7+flag/pmc06146818-339-61-34
Average 90 stars, based on 1 article reviews
pca7-flag - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Sequencing:

Article Title: The RNA- and TRIM25-Binding Domains of Influenza Virus NS1 Protein Are Essential for Suppression of NLRP3 Inflammasome-Mediated Interleukin-1β Secretion
Article Snippet: Oligonucleotides corresponding to both strands of the full-length sequence of NS1 proteins from influenza virus strains A/Brevig Mission/1/18 (H1N1) (BM/1918), A/Hong Kong/483/1997 (H5N1) (HK/1997), and A/Anhui/1-BALF_RG1/2013 (H7N9) (Anhui/2013) and containing EcoRI and NotI sites at the 5′ and 3′ ends were synthesized (Eurofins Genomics) and cloned into the eukaryotic expression vectors pCA7-Flag ( 38 ) (a derivative of pCAGGS [ 39 ]) to produce Flag-tagged proteins.

Article Title: Two Conserved Amino Acids within the NSs of Severe Fever with Thrombocytopenia Syndrome Phlebovirus Are Essential for Anti-interferon Activity
Article Snippet: Oligonucleotides corresponding to both strands of the full-length sequence of NSs from HRTV isolate patient1 (GenBank accession number NC_024496.1 ) containing EcoRI and NotI sites at the 5′ and 3′ ends, respectively, were synthesized (Eurofins Genomics) and used to amplify and clone the gene into the eukaryotic expression vectors pCA7 ( 49 ) (a derivative of pCAGGS [ 50 ]) and pCA7-Flag to produce Flag-tagged proteins.

Synthesized:

Article Title: The RNA- and TRIM25-Binding Domains of Influenza Virus NS1 Protein Are Essential for Suppression of NLRP3 Inflammasome-Mediated Interleukin-1β Secretion
Article Snippet: Oligonucleotides corresponding to both strands of the full-length sequence of NS1 proteins from influenza virus strains A/Brevig Mission/1/18 (H1N1) (BM/1918), A/Hong Kong/483/1997 (H5N1) (HK/1997), and A/Anhui/1-BALF_RG1/2013 (H7N9) (Anhui/2013) and containing EcoRI and NotI sites at the 5′ and 3′ ends were synthesized (Eurofins Genomics) and cloned into the eukaryotic expression vectors pCA7-Flag ( 38 ) (a derivative of pCAGGS [ 39 ]) to produce Flag-tagged proteins.

Article Title: Two Conserved Amino Acids within the NSs of Severe Fever with Thrombocytopenia Syndrome Phlebovirus Are Essential for Anti-interferon Activity
Article Snippet: Oligonucleotides corresponding to both strands of the full-length sequence of NSs from HRTV isolate patient1 (GenBank accession number NC_024496.1 ) containing EcoRI and NotI sites at the 5′ and 3′ ends, respectively, were synthesized (Eurofins Genomics) and used to amplify and clone the gene into the eukaryotic expression vectors pCA7 ( 49 ) (a derivative of pCAGGS [ 50 ]) and pCA7-Flag to produce Flag-tagged proteins.

Expressing:

Article Title: The RNA- and TRIM25-Binding Domains of Influenza Virus NS1 Protein Are Essential for Suppression of NLRP3 Inflammasome-Mediated Interleukin-1β Secretion
Article Snippet: Oligonucleotides corresponding to both strands of the full-length sequence of NS1 proteins from influenza virus strains A/Brevig Mission/1/18 (H1N1) (BM/1918), A/Hong Kong/483/1997 (H5N1) (HK/1997), and A/Anhui/1-BALF_RG1/2013 (H7N9) (Anhui/2013) and containing EcoRI and NotI sites at the 5′ and 3′ ends were synthesized (Eurofins Genomics) and cloned into the eukaryotic expression vectors pCA7-Flag ( 38 ) (a derivative of pCAGGS [ 39 ]) to produce Flag-tagged proteins.

Article Title: Two Conserved Amino Acids within the NSs of Severe Fever with Thrombocytopenia Syndrome Phlebovirus Are Essential for Anti-interferon Activity
Article Snippet: Oligonucleotides corresponding to both strands of the full-length sequence of NSs from HRTV isolate patient1 (GenBank accession number NC_024496.1 ) containing EcoRI and NotI sites at the 5′ and 3′ ends, respectively, were synthesized (Eurofins Genomics) and used to amplify and clone the gene into the eukaryotic expression vectors pCA7 ( 49 ) (a derivative of pCAGGS [ 50 ]) and pCA7-Flag to produce Flag-tagged proteins.

Virus:

Article Title: The RNA- and TRIM25-Binding Domains of Influenza Virus NS1 Protein Are Essential for Suppression of NLRP3 Inflammasome-Mediated Interleukin-1β Secretion
Article Snippet: Oligonucleotides corresponding to both strands of the full-length sequence of NS1 proteins from influenza virus strains A/Brevig Mission/1/18 (H1N1) (BM/1918), A/Hong Kong/483/1997 (H5N1) (HK/1997), and A/Anhui/1-BALF_RG1/2013 (H7N9) (Anhui/2013) and containing EcoRI and NotI sites at the 5′ and 3′ ends were synthesized (Eurofins Genomics) and cloned into the eukaryotic expression vectors pCA7-Flag ( 38 ) (a derivative of pCAGGS [ 39 ]) to produce Flag-tagged proteins.

Article Title: Two Conserved Amino Acids within the NSs of Severe Fever with Thrombocytopenia Syndrome Phlebovirus Are Essential for Anti-interferon Activity
Article Snippet: Oligonucleotides corresponding to both strands of the full-length sequence of NSs from HRTV isolate patient1 (GenBank accession number NC_024496.1 ) containing EcoRI and NotI sites at the 5′ and 3′ ends, respectively, were synthesized (Eurofins Genomics) and used to amplify and clone the gene into the eukaryotic expression vectors pCA7 ( 49 ) (a derivative of pCAGGS [ 50 ]) and pCA7-Flag to produce Flag-tagged proteins.

Clone Assay:

Article Title: The RNA- and TRIM25-Binding Domains of Influenza Virus NS1 Protein Are Essential for Suppression of NLRP3 Inflammasome-Mediated Interleukin-1β Secretion
Article Snippet: Oligonucleotides corresponding to both strands of the full-length sequence of NS1 proteins from influenza virus strains A/Brevig Mission/1/18 (H1N1) (BM/1918), A/Hong Kong/483/1997 (H5N1) (HK/1997), and A/Anhui/1-BALF_RG1/2013 (H7N9) (Anhui/2013) and containing EcoRI and NotI sites at the 5′ and 3′ ends were synthesized (Eurofins Genomics) and cloned into the eukaryotic expression vectors pCA7-Flag ( 38 ) (a derivative of pCAGGS [ 39 ]) to produce Flag-tagged proteins.

Article Title: Two Conserved Amino Acids within the NSs of Severe Fever with Thrombocytopenia Syndrome Phlebovirus Are Essential for Anti-interferon Activity
Article Snippet: Oligonucleotides corresponding to both strands of the full-length sequence of NSs from HRTV isolate patient1 (GenBank accession number NC_024496.1 ) containing EcoRI and NotI sites at the 5′ and 3′ ends, respectively, were synthesized (Eurofins Genomics) and used to amplify and clone the gene into the eukaryotic expression vectors pCA7 ( 49 ) (a derivative of pCAGGS [ 50 ]) and pCA7-Flag to produce Flag-tagged proteins.



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