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pc12 cells  (ATCC)


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    ATCC pc12 cells
    RNA sequencing and quality control from <t>PC12</t> cells following NGF withdrawal and replenishment. (a) Timeline of PC12 treatment and collection points. Cells were plated and cultured with NGF for 6 days before the baseline collection point. Further collections were taken 8h, 48h, 56h and 72h after baseline, with NGF withdrawn and replenished as indicated. (b) Read confidence as measured by FastQC, collated by MultiQC, for all samples both pre- and post-trimming. (c) Number of input reads and (d) uniquely mapped reads as determined by STAR. Error bars represent mean ± SD. (e) PCA was performed to visualize the variation among samples and conditions. The axes represent principal components PC1 and PC2, which account for 43% and 27% of sample variance, respectively.
    Pc12 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 4927 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pc12 cells/product/ATCC
    Average 98 stars, based on 4927 article reviews
    pc12 cells - by Bioz Stars, 2026-04
    98/100 stars

    Images

    1) Product Images from "A time-resolved RNA-sequencing dataset of transcriptional responses in PC12 cells to NGF withdrawal and replenishment"

    Article Title: A time-resolved RNA-sequencing dataset of transcriptional responses in PC12 cells to NGF withdrawal and replenishment

    Journal: Data in Brief

    doi: 10.1016/j.dib.2026.112639

    RNA sequencing and quality control from PC12 cells following NGF withdrawal and replenishment. (a) Timeline of PC12 treatment and collection points. Cells were plated and cultured with NGF for 6 days before the baseline collection point. Further collections were taken 8h, 48h, 56h and 72h after baseline, with NGF withdrawn and replenished as indicated. (b) Read confidence as measured by FastQC, collated by MultiQC, for all samples both pre- and post-trimming. (c) Number of input reads and (d) uniquely mapped reads as determined by STAR. Error bars represent mean ± SD. (e) PCA was performed to visualize the variation among samples and conditions. The axes represent principal components PC1 and PC2, which account for 43% and 27% of sample variance, respectively.
    Figure Legend Snippet: RNA sequencing and quality control from PC12 cells following NGF withdrawal and replenishment. (a) Timeline of PC12 treatment and collection points. Cells were plated and cultured with NGF for 6 days before the baseline collection point. Further collections were taken 8h, 48h, 56h and 72h after baseline, with NGF withdrawn and replenished as indicated. (b) Read confidence as measured by FastQC, collated by MultiQC, for all samples both pre- and post-trimming. (c) Number of input reads and (d) uniquely mapped reads as determined by STAR. Error bars represent mean ± SD. (e) PCA was performed to visualize the variation among samples and conditions. The axes represent principal components PC1 and PC2, which account for 43% and 27% of sample variance, respectively.

    Techniques Used: RNA Sequencing, Control, Cell Culture



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    RNA sequencing and quality control from <t>PC12</t> cells following NGF withdrawal and replenishment. (a) Timeline of PC12 treatment and collection points. Cells were plated and cultured with NGF for 6 days before the baseline collection point. Further collections were taken 8h, 48h, 56h and 72h after baseline, with NGF withdrawn and replenished as indicated. (b) Read confidence as measured by FastQC, collated by MultiQC, for all samples both pre- and post-trimming. (c) Number of input reads and (d) uniquely mapped reads as determined by STAR. Error bars represent mean ± SD. (e) PCA was performed to visualize the variation among samples and conditions. The axes represent principal components PC1 and PC2, which account for 43% and 27% of sample variance, respectively.
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    RNA sequencing and quality control from <t>PC12</t> cells following NGF withdrawal and replenishment. (a) Timeline of PC12 treatment and collection points. Cells were plated and cultured with NGF for 6 days before the baseline collection point. Further collections were taken 8h, 48h, 56h and 72h after baseline, with NGF withdrawn and replenished as indicated. (b) Read confidence as measured by FastQC, collated by MultiQC, for all samples both pre- and post-trimming. (c) Number of input reads and (d) uniquely mapped reads as determined by STAR. Error bars represent mean ± SD. (e) PCA was performed to visualize the variation among samples and conditions. The axes represent principal components PC1 and PC2, which account for 43% and 27% of sample variance, respectively.
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    RNA sequencing and quality control from <t>PC12</t> cells following NGF withdrawal and replenishment. (a) Timeline of PC12 treatment and collection points. Cells were plated and cultured with NGF for 6 days before the baseline collection point. Further collections were taken 8h, 48h, 56h and 72h after baseline, with NGF withdrawn and replenished as indicated. (b) Read confidence as measured by FastQC, collated by MultiQC, for all samples both pre- and post-trimming. (c) Number of input reads and (d) uniquely mapped reads as determined by STAR. Error bars represent mean ± SD. (e) PCA was performed to visualize the variation among samples and conditions. The axes represent principal components PC1 and PC2, which account for 43% and 27% of sample variance, respectively.
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    RNA sequencing and quality control from PC12 cells following NGF withdrawal and replenishment. (a) Timeline of PC12 treatment and collection points. Cells were plated and cultured with NGF for 6 days before the baseline collection point. Further collections were taken 8h, 48h, 56h and 72h after baseline, with NGF withdrawn and replenished as indicated. (b) Read confidence as measured by FastQC, collated by MultiQC, for all samples both pre- and post-trimming. (c) Number of input reads and (d) uniquely mapped reads as determined by STAR. Error bars represent mean ± SD. (e) PCA was performed to visualize the variation among samples and conditions. The axes represent principal components PC1 and PC2, which account for 43% and 27% of sample variance, respectively.

    Journal: Data in Brief

    Article Title: A time-resolved RNA-sequencing dataset of transcriptional responses in PC12 cells to NGF withdrawal and replenishment

    doi: 10.1016/j.dib.2026.112639

    Figure Lengend Snippet: RNA sequencing and quality control from PC12 cells following NGF withdrawal and replenishment. (a) Timeline of PC12 treatment and collection points. Cells were plated and cultured with NGF for 6 days before the baseline collection point. Further collections were taken 8h, 48h, 56h and 72h after baseline, with NGF withdrawn and replenished as indicated. (b) Read confidence as measured by FastQC, collated by MultiQC, for all samples both pre- and post-trimming. (c) Number of input reads and (d) uniquely mapped reads as determined by STAR. Error bars represent mean ± SD. (e) PCA was performed to visualize the variation among samples and conditions. The axes represent principal components PC1 and PC2, which account for 43% and 27% of sample variance, respectively.

    Article Snippet: PC12 cells were purchased from ATCC (CRL-1721) and maintained per manufacturer protocol in growth media containing RPMI01640 media (ATCC, 30-2001) supplemented 10% Heat inactivated horse serum (ATCC, 30-2004) and 5% fetal bovine serum and supplemented with 1% penicillin/ streptomycin (Lonza BioWhittaker, 17-602E) and Gentamycin (VWR, 0304-10G).

    Techniques: RNA Sequencing, Control, Cell Culture