Journal: Cellular and Molecular Gastroenterology and Hepatology
Article Title: Loss of Brg1 and Pten in Pancreatic Ductal Cells Forms Intraductal Tubulopapillary Neoplasm via the YAP/TAZ Pathway
doi: 10.1016/j.jcmgh.2025.101639
Figure Lengend Snippet: Evaluation of SWI/SNF components and TAZ expression in human ITPN samples. ( A ) Expression of SWI/SNF components, including BRG1, BRM, ARID1A, and PBRM1, in human ITPN specimens. Additionally, the expression levels of pAKT and TAZ were analyzed. Black, blue, green, and red indicate negative, weak, moderate, and strong expression, respectively. Cases with reduced expression of any SWI/SNF complex component are shown in yellow . ( B ) Summary of BRG1 staining for human IPMN and ITPN samples. Immunohistochemistry for BRG1 in IPMN (n = 20), ITPN (n = 12), and adjacent normal pancreas (n = 3) specimens. Images of the adjacent normal pancreas were captured from the same tissue slide as IPMN or ITPN, if available. Scale bar, 20 mm. Tables on the right show the number of samples classified as ITPN or IPMN and their staining intensity. Statistical analysis was performed using the Mann–Whitney U test. ( C ) Analysis of Spearman’s correlation coefficients and scatterplots with jitter and linear regression. There was a significant negative correlation between BRG1 and TAZ expression (−0.803). There was a moderate negative correlation between ARID1A or BRM and TAZ expression (−0.422 and −0.518, respectively). No correlation was observed between PBRM1 or pAKT and TAZ expression (0.147 and 0.125, respectively). ( D ) Representative cases are shown. Scale bar, 20 μm.
Article Snippet: PBRM1 , Bethyl , 1:500 , A301-591A.
Techniques: Expressing, Staining, Immunohistochemistry, MANN-WHITNEY
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