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pathological structure changes  (Novus Biologicals)


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    Novus Biologicals pathological structure changes
    Wogonin inhibited TLR4 to ameliorate renal injury via upregulation of SOCS3 in db/db mice. db/db mice were injected with adenovirus sh-SOCS3 or sh-SOCS3 + sh-TLR4, followed by treatment by vehicle or wogonin. ( A ) Blood glucose levels were measured using an automated chemistry analyzer. ( B ) The <t>pathological</t> features and glucose in the renal tissues were evaluated by hematoxylin, eosin (HE), and periodic acid-Schiff base (PAS). Scale bar = 50 μm. ( C - D ) The urinary protein, serum creatinine values (Scr), and blood urea nitrogen (BUN) were evaluated by an automated chemistry analyzer. ( E - F ) 24-hour albumin and urinary albumin-to-creatinine ratio (ACR) were detected by ELISA assay in HG mice without or with wogonin, wogonin + sh-SOCS3, wogonin + sh-SOCS3 + sh-TLR4 ( E ) ELISA evaluated the levels of cytokines. ( F - G ) The mRNA and protein levels of SOCS3 and TLR4 were detected by RT-qPCR and western blot, respectively. ( H ) The protein levels of collagen I, CTGF, FN, and TGF-β1 were detected by western blot assay. ( I ) The protein levels of ACE, Ang II, and AT1R were detected by western blot assay. ( J ) The protein levels of p-JAK2, JAK2, p-STAT1, STAT1, p-STAT3, STAT3, AIM2, ASC, caspase-1, IL-18 and IL-1β were detected by western blot assay. * P < 0.05, ** P < 0.01, *** P < 0.001 versus the corresponding control. Data are representative of three independent experiments
    Pathological Structure Changes, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pathological+structure+changes/Hematoxylin+and+Eosin+Stain+Kit/pmc11155057-87-9-14
    Average 94 stars, based on 10 article reviews
    pathological structure changes - by Bioz Stars, 2026-09
    94/100 stars

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    1) Product Images from "Wogonin upregulates SOCS3 to alleviate the injury in Diabetic Nephropathy by inhibiting TLR4-mediated JAK/STAT/AIM2 signaling pathway"

    Article Title: Wogonin upregulates SOCS3 to alleviate the injury in Diabetic Nephropathy by inhibiting TLR4-mediated JAK/STAT/AIM2 signaling pathway

    Journal: Molecular Medicine

    doi: 10.1186/s10020-024-00845-4

    Wogonin inhibited TLR4 to ameliorate renal injury via upregulation of SOCS3 in db/db mice. db/db mice were injected with adenovirus sh-SOCS3 or sh-SOCS3 + sh-TLR4, followed by treatment by vehicle or wogonin. ( A ) Blood glucose levels were measured using an automated chemistry analyzer. ( B ) The pathological features and glucose in the renal tissues were evaluated by hematoxylin, eosin (HE), and periodic acid-Schiff base (PAS). Scale bar = 50 μm. ( C - D ) The urinary protein, serum creatinine values (Scr), and blood urea nitrogen (BUN) were evaluated by an automated chemistry analyzer. ( E - F ) 24-hour albumin and urinary albumin-to-creatinine ratio (ACR) were detected by ELISA assay in HG mice without or with wogonin, wogonin + sh-SOCS3, wogonin + sh-SOCS3 + sh-TLR4 ( E ) ELISA evaluated the levels of cytokines. ( F - G ) The mRNA and protein levels of SOCS3 and TLR4 were detected by RT-qPCR and western blot, respectively. ( H ) The protein levels of collagen I, CTGF, FN, and TGF-β1 were detected by western blot assay. ( I ) The protein levels of ACE, Ang II, and AT1R were detected by western blot assay. ( J ) The protein levels of p-JAK2, JAK2, p-STAT1, STAT1, p-STAT3, STAT3, AIM2, ASC, caspase-1, IL-18 and IL-1β were detected by western blot assay. * P < 0.05, ** P < 0.01, *** P < 0.001 versus the corresponding control. Data are representative of three independent experiments
    Figure Legend Snippet: Wogonin inhibited TLR4 to ameliorate renal injury via upregulation of SOCS3 in db/db mice. db/db mice were injected with adenovirus sh-SOCS3 or sh-SOCS3 + sh-TLR4, followed by treatment by vehicle or wogonin. ( A ) Blood glucose levels were measured using an automated chemistry analyzer. ( B ) The pathological features and glucose in the renal tissues were evaluated by hematoxylin, eosin (HE), and periodic acid-Schiff base (PAS). Scale bar = 50 μm. ( C - D ) The urinary protein, serum creatinine values (Scr), and blood urea nitrogen (BUN) were evaluated by an automated chemistry analyzer. ( E - F ) 24-hour albumin and urinary albumin-to-creatinine ratio (ACR) were detected by ELISA assay in HG mice without or with wogonin, wogonin + sh-SOCS3, wogonin + sh-SOCS3 + sh-TLR4 ( E ) ELISA evaluated the levels of cytokines. ( F - G ) The mRNA and protein levels of SOCS3 and TLR4 were detected by RT-qPCR and western blot, respectively. ( H ) The protein levels of collagen I, CTGF, FN, and TGF-β1 were detected by western blot assay. ( I ) The protein levels of ACE, Ang II, and AT1R were detected by western blot assay. ( J ) The protein levels of p-JAK2, JAK2, p-STAT1, STAT1, p-STAT3, STAT3, AIM2, ASC, caspase-1, IL-18 and IL-1β were detected by western blot assay. * P < 0.05, ** P < 0.01, *** P < 0.001 versus the corresponding control. Data are representative of three independent experiments

    Techniques Used: Injection, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot, Control

    Related Articles

    Injection:

    Article Title: Wogonin upregulates SOCS3 to alleviate the injury in Diabetic Nephropathy by inhibiting TLR4-mediated JAK/STAT/AIM2 signaling pathway
    Article Snippet: The sections were conducted into 10 μm thickness.The sections were conducted into 10 μm thickness.. Subsequently, hematoxylin and eosin were used to analyze the pathological structure changes (H&E, H-3502-NB, Novus Biologicals, LLC, CO, USA).. The glycogen levels in tissues were evaluated using a periodic acid-Schiff (PAS) stain kit (AR165, Agilent, CA, USA).The glycogen levels in tissues were evaluated using a periodic acid-Schiff (PAS) stain kit (AR165, Agilent, CA, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Wogonin upregulates SOCS3 to alleviate the injury in Diabetic Nephropathy by inhibiting TLR4-mediated JAK/STAT/AIM2 signaling pathway
    Article Snippet: The sections were conducted into 10 μm thickness.The sections were conducted into 10 μm thickness.. Subsequently, hematoxylin and eosin were used to analyze the pathological structure changes (H&E, H-3502-NB, Novus Biologicals, LLC, CO, USA).. The glycogen levels in tissues were evaluated using a periodic acid-Schiff (PAS) stain kit (AR165, Agilent, CA, USA).The glycogen levels in tissues were evaluated using a periodic acid-Schiff (PAS) stain kit (AR165, Agilent, CA, USA).

    Quantitative RT-PCR:

    Article Title: Wogonin upregulates SOCS3 to alleviate the injury in Diabetic Nephropathy by inhibiting TLR4-mediated JAK/STAT/AIM2 signaling pathway
    Article Snippet: The sections were conducted into 10 μm thickness.The sections were conducted into 10 μm thickness.. Subsequently, hematoxylin and eosin were used to analyze the pathological structure changes (H&E, H-3502-NB, Novus Biologicals, LLC, CO, USA).. The glycogen levels in tissues were evaluated using a periodic acid-Schiff (PAS) stain kit (AR165, Agilent, CA, USA).The glycogen levels in tissues were evaluated using a periodic acid-Schiff (PAS) stain kit (AR165, Agilent, CA, USA).

    Western Blot:

    Article Title: Wogonin upregulates SOCS3 to alleviate the injury in Diabetic Nephropathy by inhibiting TLR4-mediated JAK/STAT/AIM2 signaling pathway
    Article Snippet: The sections were conducted into 10 μm thickness.The sections were conducted into 10 μm thickness.. Subsequently, hematoxylin and eosin were used to analyze the pathological structure changes (H&E, H-3502-NB, Novus Biologicals, LLC, CO, USA).. The glycogen levels in tissues were evaluated using a periodic acid-Schiff (PAS) stain kit (AR165, Agilent, CA, USA).The glycogen levels in tissues were evaluated using a periodic acid-Schiff (PAS) stain kit (AR165, Agilent, CA, USA).

    Control:

    Article Title: Wogonin upregulates SOCS3 to alleviate the injury in Diabetic Nephropathy by inhibiting TLR4-mediated JAK/STAT/AIM2 signaling pathway
    Article Snippet: The sections were conducted into 10 μm thickness.The sections were conducted into 10 μm thickness.. Subsequently, hematoxylin and eosin were used to analyze the pathological structure changes (H&E, H-3502-NB, Novus Biologicals, LLC, CO, USA).. The glycogen levels in tissues were evaluated using a periodic acid-Schiff (PAS) stain kit (AR165, Agilent, CA, USA).The glycogen levels in tissues were evaluated using a periodic acid-Schiff (PAS) stain kit (AR165, Agilent, CA, USA).



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    Wogonin inhibited TLR4 to ameliorate renal injury via upregulation of SOCS3 in db/db mice. db/db mice were injected with adenovirus sh-SOCS3 or sh-SOCS3 + sh-TLR4, followed by treatment by vehicle or wogonin. ( A ) Blood glucose levels were measured using an automated chemistry analyzer. ( B ) The pathological features and glucose in the renal tissues were evaluated by hematoxylin, eosin (HE), and periodic acid-Schiff base (PAS). Scale bar = 50 μm. ( C - D ) The urinary protein, serum creatinine values (Scr), and blood urea nitrogen (BUN) were evaluated by an automated chemistry analyzer. ( E - F ) 24-hour albumin and urinary albumin-to-creatinine ratio (ACR) were detected by ELISA assay in HG mice without or with wogonin, wogonin + sh-SOCS3, wogonin + sh-SOCS3 + sh-TLR4 ( E ) ELISA evaluated the levels of cytokines. ( F - G ) The mRNA and protein levels of SOCS3 and TLR4 were detected by RT-qPCR and western blot, respectively. ( H ) The protein levels of collagen I, CTGF, FN, and TGF-β1 were detected by western blot assay. ( I ) The protein levels of ACE, Ang II, and AT1R were detected by western blot assay. ( J ) The protein levels of p-JAK2, JAK2, p-STAT1, STAT1, p-STAT3, STAT3, AIM2, ASC, caspase-1, IL-18 and IL-1β were detected by western blot assay. * P < 0.05, ** P < 0.01, *** P < 0.001 versus the corresponding control. Data are representative of three independent experiments

    Journal: Molecular Medicine

    Article Title: Wogonin upregulates SOCS3 to alleviate the injury in Diabetic Nephropathy by inhibiting TLR4-mediated JAK/STAT/AIM2 signaling pathway

    doi: 10.1186/s10020-024-00845-4

    Figure Lengend Snippet: Wogonin inhibited TLR4 to ameliorate renal injury via upregulation of SOCS3 in db/db mice. db/db mice were injected with adenovirus sh-SOCS3 or sh-SOCS3 + sh-TLR4, followed by treatment by vehicle or wogonin. ( A ) Blood glucose levels were measured using an automated chemistry analyzer. ( B ) The pathological features and glucose in the renal tissues were evaluated by hematoxylin, eosin (HE), and periodic acid-Schiff base (PAS). Scale bar = 50 μm. ( C - D ) The urinary protein, serum creatinine values (Scr), and blood urea nitrogen (BUN) were evaluated by an automated chemistry analyzer. ( E - F ) 24-hour albumin and urinary albumin-to-creatinine ratio (ACR) were detected by ELISA assay in HG mice without or with wogonin, wogonin + sh-SOCS3, wogonin + sh-SOCS3 + sh-TLR4 ( E ) ELISA evaluated the levels of cytokines. ( F - G ) The mRNA and protein levels of SOCS3 and TLR4 were detected by RT-qPCR and western blot, respectively. ( H ) The protein levels of collagen I, CTGF, FN, and TGF-β1 were detected by western blot assay. ( I ) The protein levels of ACE, Ang II, and AT1R were detected by western blot assay. ( J ) The protein levels of p-JAK2, JAK2, p-STAT1, STAT1, p-STAT3, STAT3, AIM2, ASC, caspase-1, IL-18 and IL-1β were detected by western blot assay. * P < 0.05, ** P < 0.01, *** P < 0.001 versus the corresponding control. Data are representative of three independent experiments

    Article Snippet: Subsequently, hematoxylin and eosin were used to analyze the pathological structure changes (H&E, H-3502-NB, Novus Biologicals, LLC, CO, USA).

    Techniques: Injection, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot, Control