padi2 polyclonal antibody  (Proteintech)

Journal: Particle and Fibre Toxicology

Article Title: Inhibition of PADI2-mediated vimentin citrullination alleviates silica-induced pulmonary fibrosis in mice

doi: 10.1186/s12989-026-00671-y

Figure Lengend Snippet: Upregulation of PADI2, Citrullinated Peptides, and Citrullinated Vimentin in a silicosis model. A – F Immunofluorescence staining of Cit-peptide and vimentin (Vim) co-expression in silicotic rats and mice (scale bar = 50 μm). Western blot analysis showing the expression levels of PADI2, Cit-peptide, and Cit-Vim across groups (Results are presented as Mean ± SD, n = 6 per group). G – H Immunohistochemical staining of PADI2 and immunofluorescence staining of Cit-peptide and Vim co-expression in macrophage-depleted mice (scale bar = 50 μm). Western blot analysis of PADI2, Cit-peptide, and Cit-Vim expression (Results are presented as Mean ± SD, n = 3 per group)

Article Snippet: Padi2 flox/flox; Lyz2−cre mice were generated and obtained from Cyagen Biosciences Inc.

Techniques: Immunofluorescence, Staining, Expressing, Western Blot, Immunohistochemical staining

Journal: Particle and Fibre Toxicology

Article Title: Inhibition of PADI2-mediated vimentin citrullination alleviates silica-induced pulmonary fibrosis in mice

doi: 10.1186/s12989-026-00671-y

Figure Lengend Snippet: Attenuation of Silicosis-induced Fibrosis and protection of lung function by PADI2 inhibition. A H&E and VG staining in Padi2 knockout mice (scale bars = 1–100 μm) (Results are presented as Mean ± SD, n = 6 per group). B Micro CT imaging of lung tissue in Padi2 knockout mice (Results are presented as Mean ± SD, n = 6 per group). C Western blot analysis of pro-CoL I and FN1 expression in Padi2 knockout mice (Results are presented as Mean ± SD, n = 3 per group). D Pulmonary Function Assessment in Padi2 knockout mice (Results are presented as Mean ± SD, n = 6 per group)

Article Snippet: Padi2 flox/flox; Lyz2−cre mice were generated and obtained from Cyagen Biosciences Inc.

Techniques: Inhibition, Staining, Knock-Out, Micro-CT, Imaging, Western Blot, Expressing

Journal: Particle and Fibre Toxicology

Article Title: Inhibition of PADI2-mediated vimentin citrullination alleviates silica-induced pulmonary fibrosis in mice

doi: 10.1186/s12989-026-00671-y

Figure Lengend Snippet: PADI2 regulates citrullinated peptides and citrullinated vimentin expression. A Immunofluorescence staining of Cit-peptide and Vim co-expression (scale bar = 50 μm) and Western blot analysis of PADI2, Cit-peptide, and Cit-Vim expression in AFM-30a-treated mice. B Immunofluorescence staining of Cit-peptide and Vim co-expression (scale bar = 100 μm) and Western blot analysis of PADI2, Cit-peptide, and Cit-Vim expression in Padi2 knockout mice. Results are presented as Mean ± SD, n = 3 per group

Article Snippet: Padi2 flox/flox; Lyz2−cre mice were generated and obtained from Cyagen Biosciences Inc.

Techniques: Expressing, Immunofluorescence, Staining, Western Blot, Knock-Out

Journal: Particle and Fibre Toxicology

Article Title: Inhibition of PADI2-mediated vimentin citrullination alleviates silica-induced pulmonary fibrosis in mice

doi: 10.1186/s12989-026-00671-y

Figure Lengend Snippet: Transcriptome Sequencing Reveals pathways and targets of Padi2 knock in Mitigating Pulmonary Fibrosis in Silicotic Mice. A Hierarchical cluster heatmaps showing gene expression in different groups. B – C GO and KEGG pathway analysis of downregulated and upregulated genes in the Padi2 knockout (KOS) group. D Heatmaps displaying significantly downregulated genes in the KOS Group. E – F Immunofluorescence staining of TNF-α expression (scale bar = 100 μm) in RAW 264.7 cells treated with Cit-Vim. G – H Western blot analysis of EGR1, TNF-α, IL-6 and IL-1β expression in RAW 264.7 cells treated with Cit-Vim. I – J Western blot analysis of TNF-α, IL-6 and IL-1β expression in Cit-Vim and siRNA- Egr1 -treated RAW 264.7 cells. Results are presented as Mean ± SD, n = 3 per group

Article Snippet: Padi2 flox/flox; Lyz2−cre mice were generated and obtained from Cyagen Biosciences Inc.

Techniques: Sequencing, Knock-In, Gene Expression, Knock-Out, Immunofluorescence, Staining, Expressing, Western Blot

Journal: Particle and Fibre Toxicology

Article Title: Inhibition of PADI2-mediated vimentin citrullination alleviates silica-induced pulmonary fibrosis in mice

doi: 10.1186/s12989-026-00671-y

Figure Lengend Snippet: PADI2 regulates inflammatory response in silicosis. A – B Immunofluorescence staining of EGR1 and TNF-α expression (scale bar = 100 μm) and Western blot analysis of EGR1 and TNF-α, IL-6 and IL-1β expression in Padi2 knockout mice. Results are presented as Mean ± SD, n = 3 per group

Article Snippet: Padi2 flox/flox; Lyz2−cre mice were generated and obtained from Cyagen Biosciences Inc.

Techniques: Immunofluorescence, Staining, Expressing, Western Blot, Knock-Out

Journal: Particle and Fibre Toxicology

Article Title: Inhibition of PADI2-mediated vimentin citrullination alleviates silica-induced pulmonary fibrosis in mice

doi: 10.1186/s12989-026-00671-y

Figure Lengend Snippet: Upregulation of PADI2, Citrullinated Peptides, and Citrullinated Vimentin in a silicosis model. A – F Immunofluorescence staining of Cit-peptide and vimentin (Vim) co-expression in silicotic rats and mice (scale bar = 50 μm). Western blot analysis showing the expression levels of PADI2, Cit-peptide, and Cit-Vim across groups (Results are presented as Mean ± SD, n = 6 per group). G – H Immunohistochemical staining of PADI2 and immunofluorescence staining of Cit-peptide and Vim co-expression in macrophage-depleted mice (scale bar = 50 μm). Western blot analysis of PADI2, Cit-peptide, and Cit-Vim expression (Results are presented as Mean ± SD, n = 3 per group)

Article Snippet: Cells were treated with silica (50, 100–200 μg/mL), or transfected with siRNAs targeting scavenger receptor B 1 ( Srb1 , NO 327; RIBOBIO, Guangzhou, China), Padi2 (sc-61282; Santa cruz, CA, USA) or Early Growth Response Protein 1 ( Egr1 , sc-35267; Santa cruz, CA, USA).

Techniques: Immunofluorescence, Staining, Expressing, Western Blot, Immunohistochemical staining

Journal: Particle and Fibre Toxicology

Article Title: Inhibition of PADI2-mediated vimentin citrullination alleviates silica-induced pulmonary fibrosis in mice

doi: 10.1186/s12989-026-00671-y

Figure Lengend Snippet: Attenuation of Silicosis-induced Fibrosis and protection of lung function by PADI2 inhibition. A H&E and VG staining in Padi2 knockout mice (scale bars = 1–100 μm) (Results are presented as Mean ± SD, n = 6 per group). B Micro CT imaging of lung tissue in Padi2 knockout mice (Results are presented as Mean ± SD, n = 6 per group). C Western blot analysis of pro-CoL I and FN1 expression in Padi2 knockout mice (Results are presented as Mean ± SD, n = 3 per group). D Pulmonary Function Assessment in Padi2 knockout mice (Results are presented as Mean ± SD, n = 6 per group)

Article Snippet: Cells were treated with silica (50, 100–200 μg/mL), or transfected with siRNAs targeting scavenger receptor B 1 ( Srb1 , NO 327; RIBOBIO, Guangzhou, China), Padi2 (sc-61282; Santa cruz, CA, USA) or Early Growth Response Protein 1 ( Egr1 , sc-35267; Santa cruz, CA, USA).

Techniques: Inhibition, Staining, Knock-Out, Micro-CT, Imaging, Western Blot, Expressing

Journal: Particle and Fibre Toxicology

Article Title: Inhibition of PADI2-mediated vimentin citrullination alleviates silica-induced pulmonary fibrosis in mice

doi: 10.1186/s12989-026-00671-y

Figure Lengend Snippet: PADI2 regulates citrullinated peptides and citrullinated vimentin expression. A Immunofluorescence staining of Cit-peptide and Vim co-expression (scale bar = 50 μm) and Western blot analysis of PADI2, Cit-peptide, and Cit-Vim expression in AFM-30a-treated mice. B Immunofluorescence staining of Cit-peptide and Vim co-expression (scale bar = 100 μm) and Western blot analysis of PADI2, Cit-peptide, and Cit-Vim expression in Padi2 knockout mice. Results are presented as Mean ± SD, n = 3 per group

Article Snippet: Cells were treated with silica (50, 100–200 μg/mL), or transfected with siRNAs targeting scavenger receptor B 1 ( Srb1 , NO 327; RIBOBIO, Guangzhou, China), Padi2 (sc-61282; Santa cruz, CA, USA) or Early Growth Response Protein 1 ( Egr1 , sc-35267; Santa cruz, CA, USA).

Techniques: Expressing, Immunofluorescence, Staining, Western Blot, Knock-Out

Journal: Particle and Fibre Toxicology

Article Title: Inhibition of PADI2-mediated vimentin citrullination alleviates silica-induced pulmonary fibrosis in mice

doi: 10.1186/s12989-026-00671-y

Figure Lengend Snippet: Transcriptome Sequencing Reveals pathways and targets of Padi2 knock in Mitigating Pulmonary Fibrosis in Silicotic Mice. A Hierarchical cluster heatmaps showing gene expression in different groups. B – C GO and KEGG pathway analysis of downregulated and upregulated genes in the Padi2 knockout (KOS) group. D Heatmaps displaying significantly downregulated genes in the KOS Group. E – F Immunofluorescence staining of TNF-α expression (scale bar = 100 μm) in RAW 264.7 cells treated with Cit-Vim. G – H Western blot analysis of EGR1, TNF-α, IL-6 and IL-1β expression in RAW 264.7 cells treated with Cit-Vim. I – J Western blot analysis of TNF-α, IL-6 and IL-1β expression in Cit-Vim and siRNA- Egr1 -treated RAW 264.7 cells. Results are presented as Mean ± SD, n = 3 per group

Article Snippet: Cells were treated with silica (50, 100–200 μg/mL), or transfected with siRNAs targeting scavenger receptor B 1 ( Srb1 , NO 327; RIBOBIO, Guangzhou, China), Padi2 (sc-61282; Santa cruz, CA, USA) or Early Growth Response Protein 1 ( Egr1 , sc-35267; Santa cruz, CA, USA).

Techniques: Sequencing, Knock-In, Gene Expression, Knock-Out, Immunofluorescence, Staining, Expressing, Western Blot

Journal: Particle and Fibre Toxicology

Article Title: Inhibition of PADI2-mediated vimentin citrullination alleviates silica-induced pulmonary fibrosis in mice

doi: 10.1186/s12989-026-00671-y

Figure Lengend Snippet: PADI2 regulates inflammatory response in silicosis. A – B Immunofluorescence staining of EGR1 and TNF-α expression (scale bar = 100 μm) and Western blot analysis of EGR1 and TNF-α, IL-6 and IL-1β expression in Padi2 knockout mice. Results are presented as Mean ± SD, n = 3 per group

Article Snippet: Cells were treated with silica (50, 100–200 μg/mL), or transfected with siRNAs targeting scavenger receptor B 1 ( Srb1 , NO 327; RIBOBIO, Guangzhou, China), Padi2 (sc-61282; Santa cruz, CA, USA) or Early Growth Response Protein 1 ( Egr1 , sc-35267; Santa cruz, CA, USA).

Techniques: Immunofluorescence, Staining, Expressing, Western Blot, Knock-Out

Journal: Biology of Reproduction

Article Title: Ezrin, radixin, and moesin are novel citrullinated proteins in the decidua during pregnancy ^†

doi: 10.1093/biolre/ioaf241

Figure Lengend Snippet: Complementary expression patterns of PADI2 and PADI4 in the decidua. (A and C) Immunostaining with anti-PADI2 antibody on day 7.5 pc wild-type (A) and Sphk1 −/− Sphk2 +/− (C) uteri. (B and D) Immunostaining with anti-PADI4 antibody on day 7.5 pc wild-type (B) and Sphk1 −/− Sphk2 +/− (D) uteri. The middle and lower panels show high-power views of the boxed areas from the corresponding upper panels. Em, embryo. Scale bars in panels A–D represent 500 μm (upper panels) and 50 μm (middle and lower panels). Data are representative of three independent experiments with similar results.

Article Snippet: The specimens were then incubated overnight at 4°C with rabbit anti-PADI2 (Proteintech, Rosemont, IL, USA, #12110-1-AP, RRID: AB_2159475), rabbit anti-PADI4 (Proteintech, #17373-1-AP, RRID: AB_2878398), rabbit anti-radixin (Abcam, Cambridge, UK, #ab52495, RRID: AB_882259), rabbit anti-ezrin (Abcam, #ab41672, RRID: AB_941504), rabbit anti-moesin (Abcam, #ab52490, RRID: AB_881245), or rabbit anti-ezrin (pThr567)/radixin (pThr564)/moesin (pThr558) (Abcam, #ab76247, RRID: AB_1523584) antibodies at a 1:100 dilution.

Techniques: Expressing, Immunostaining

Journal: Scientific Reports

Article Title: Peptidylarginine deiminase 2 contributes to pathogenesis in trinitrobenzenesulfonic acid-induced colitis through macrophage extracellular trap-independent pathways

doi: 10.1038/s41598-025-24221-2

Figure Lengend Snippet: Generation of PAD2KO mice using the CRISPR-Cas9 systems and pathogenesis of TNBS-induced colitis in wild-type and PAD2KO mice. ( a ) The sequences produced for the generation of PAD2KO mice using the CRISPR-Cas9 systems, deficient in 61-bp on exon2 of the genomic PAD2 DNA. ( b ) PAD2 mRNA level was determined in intraperitoneal macrophages by qRT-PCR normalized to TBP. Data were presented as the means ± S.E.M. (n = 8). ****P < 0.0001 vs. wild-type (WT) mice. ( c ) PAD2 expression was determined in intraperitoneal macrophages by western blotting normalized to β-actin expression. Data are presented as the means ± S.E.M. (n = 3). ***P < 0.001 vs. WT mice. ( d ) Body weight was measured daily in untreated-, vehicle (30% ethanol)-, or TNBS-injected WT and PAD2KO mice. On day 3, colon length was measured ( e ), representative images were obtained ( f ), and macroscopic analyses of the injured area were carried out using Image J ( g ). Scale bars are 10 mm. Histological changes were observed following H&E staining ( h ). ( i ) MPO activity was determined in those mice. Data are presented as the means ± S.E.M. (n = 8–9). #### P < 0.0001 vs. vehicle-injected mice (WT EtOH), and ****P < 0.0001, ***P < 0.001, *P < 0.05 vs. TNBS-injected mice (WT TNBS).

Article Snippet: The membranes were then incubated with PAD2 antibody (1:500) (Proteintech, 12110-1-AP) and β-actin antibody (1:2000) (CST, #4970).

Techniques: CRISPR, Produced, Quantitative RT-PCR, Expressing, Western Blot, Injection, Staining, Activity Assay

Journal: Scientific Reports

Article Title: Peptidylarginine deiminase 2 contributes to pathogenesis in trinitrobenzenesulfonic acid-induced colitis through macrophage extracellular trap-independent pathways

doi: 10.1038/s41598-025-24221-2

Figure Lengend Snippet: Expression of inflammatory cytokines, chemokines, and PAD2 in PAD2KO mice. ( a ) TNFα, ( b ) IL-1β, ( c ) IL-12, ( d ) IL-23, ( e ) IFNγ, ( f ) CXCL2, and ( g ) PAD2 mRNA expression were determined using qRT-PCR in colon tissues of vehicle (30% ethanol)-, or TNBS-injected WT and PAD2KO mice on day 3, and mRNA levels were normalized to TBP. Data are presented as the means ± S.E.M. (n = 8–10). #### P < 0.0001, ### P < 0.001, ## P < 0.01 vs. vehicle-injected mice (WT EtOH), and ****P < 0.0001, *P < 0.05 vs. TNBS-injected mice (WT TNBS).

Article Snippet: The membranes were then incubated with PAD2 antibody (1:500) (Proteintech, 12110-1-AP) and β-actin antibody (1:2000) (CST, #4970).

Techniques: Expressing, Quantitative RT-PCR, Injection