pcdna p65 flag wt mouse addgene addgene (Addgene inc)
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pcdna p65 flag wt mouse addgene addgene
Pcdna P65 Flag Wt Mouse Addgene Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p65/RelA+cFlag+pcDNA3+(Plasmid+%2320012)/pm42905514-230-215-216
Average 93 stars, based on 62 article reviews
Pcdna P65 Flag Wt Mouse Addgene Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p65/RelA+cFlag+pcDNA3+(Plasmid+%2320012)/pm42905514-230-215-216
Average 93 stars, based on 62 article reviews
pcdna p65 flag wt mouse addgene addgene - by Bioz Stars,
2026-11
93/100 stars
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Functional Assay:Article Title: Potential of omega-3 and conjugated fatty acids to control microglia inflammatory imbalance elicited by obesogenic nutrients. Article Snippet: .. The measurement of the nuclear accumulation of the p65 subunit of NF-κB as a functional indicator of NF-κB activation was achieved by using the Activation Assay:Article Title: Potential of omega-3 and conjugated fatty acids to control microglia inflammatory imbalance elicited by obesogenic nutrients. Article Snippet: .. The measurement of the nuclear accumulation of the p65 subunit of NF-κB as a functional indicator of NF-κB activation was achieved by using the Plasmid Preparation:Article Title: Potential of omega-3 and conjugated fatty acids to control microglia inflammatory imbalance elicited by obesogenic nutrients. Article Snippet: .. The measurement of the nuclear accumulation of the p65 subunit of NF-κB as a functional indicator of NF-κB activation was achieved by using the Article Title: MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo Article Snippet: .. Plasmid Construction 1) pHRdSV40-scFv-GCN4-sfGFP-VPR-GB1-NLS To construct the VPR system, the sequences VP64 (PCR amplified from the Addgene plasmid, # 60904), other:Article Title: Daily alcohol intake triggers aberrant synaptic pruning leading to synapse loss and anxiety-like behavior. Article Snippet: Plasmids used in this study were purchased from Addgene: NF-B GFP–tagged p65 (RRID:Addgene_23255), IB-miRFP703 (RRID:Addgene_80005), pLNCX chick src Y527F (RRID:Addgene_13660), pUSE-RapR-Src-myc (RRID:Addgene_25933), Control:Article Title: The NF-κB-HE4 axis: A novel regulator of HE4 secretion in ovarian cancer Article Snippet: Other materials were obtained from venders as follows; cytokines: TNFα (R&D Systems cat. No. 210-TA-020), IL-1β (BioLegend cat. No. 579402); chemicals: TPCA-1 (BioVision cat. No. 2431-5), BIRB-769 (APExBIO cat. No. A5639), JNK-IN-8 (APExBIO cat. No. A3520), CI-1040 (Selleckchem cat. No. S1020); siRNAs: Silencer Select siRNAs (ThermoFisher) for control (cat. No. 4390843) and HE4 (ID No. s20356). siGENOME siRNAs (horizon) for control (cat. No. D-001210-03-05), HE4 (cat. No. M-010564-02), and p65 (cat. No. M-003533-02). .. Santa Cruz Biotechnology siRNAs for control (cat. No. sc-37007) and HE4 (cat. No. sc-43826); Vectors: empty (addgene, cat. No. 24165), Activity Assay:Article Title: A red light-responsive photoswitch for deep tissue optogenetics. Article Snippet: All constructs encoding sgRNAs were cloned into the pSPgRNA vector (Addgene, 47108). cDNA encoding codon-optimized Streptococcus pyogenes Cas9 was amplified from pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene, 42230). .. To eliminate the nuclease activity of Cas9, we introduced D10A and H840A mutations. cDNAs encoding the MS2 (N55K) mutant and p65–HSF1 were amplified from Mutagenesis:Article Title: A red light-responsive photoswitch for deep tissue optogenetics. Article Snippet: All constructs encoding sgRNAs were cloned into the pSPgRNA vector (Addgene, 47108). cDNA encoding codon-optimized Streptococcus pyogenes Cas9 was amplified from pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene, 42230). .. To eliminate the nuclease activity of Cas9, we introduced D10A and H840A mutations. cDNAs encoding the MS2 (N55K) mutant and p65–HSF1 were amplified from Amplification:Article Title: A red light-responsive photoswitch for deep tissue optogenetics. Article Snippet: All constructs encoding sgRNAs were cloned into the pSPgRNA vector (Addgene, 47108). cDNA encoding codon-optimized Streptococcus pyogenes Cas9 was amplified from pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene, 42230). .. To eliminate the nuclease activity of Cas9, we introduced D10A and H840A mutations. cDNAs encoding the MS2 (N55K) mutant and p65–HSF1 were amplified from Article Title: MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo Article Snippet: .. Plasmid Construction 1) pHRdSV40-scFv-GCN4-sfGFP-VPR-GB1-NLS To construct the VPR system, the sequences VP64 (PCR amplified from the Addgene plasmid, # 60904), Luciferase:Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression Article Snippet: The plasmid encoding the N-terminal fragment of TonEBP generated by SARS-CoV-2 NSP5 cleavage was cloned into the pCMV-tag 2B vector (#211172, Agilent Technologies, Santa Clara, CA, USA) by amplification and standard restriction cloning. .. The IFN-β promoter luciferase reporter (#102597), as well as transient expression vectors for MDA5 (#52876), MAVS (#52135), TBK1 (#131792), IRF3 (#127663), and Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression. Article Snippet: The plasmid encoding the N-terminal fragment of TonEBP generated by SARS-CoV-2 NSP5 cleavage was cloned into the pCMV-tag 2B vector (#211172, Agilent Technologies, Santa Clara, CA, USA) by amplification and standard restriction cloning. .. The IFN-β promoter luciferase reporter (#102597), as well as transient expression vectors for MDA5 (#52876), MAVS (#52135), TBK1 (#131792), IRF3 (#127663), and Expressing:Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression Article Snippet: The plasmid encoding the N-terminal fragment of TonEBP generated by SARS-CoV-2 NSP5 cleavage was cloned into the pCMV-tag 2B vector (#211172, Agilent Technologies, Santa Clara, CA, USA) by amplification and standard restriction cloning. .. The IFN-β promoter luciferase reporter (#102597), as well as transient expression vectors for MDA5 (#52876), MAVS (#52135), TBK1 (#131792), IRF3 (#127663), and Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression. Article Snippet: The plasmid encoding the N-terminal fragment of TonEBP generated by SARS-CoV-2 NSP5 cleavage was cloned into the pCMV-tag 2B vector (#211172, Agilent Technologies, Santa Clara, CA, USA) by amplification and standard restriction cloning. .. The IFN-β promoter luciferase reporter (#102597), as well as transient expression vectors for MDA5 (#52876), MAVS (#52135), TBK1 (#131792), IRF3 (#127663), and Construct:Article Title: MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo Article Snippet: .. Plasmid Construction 1) pHRdSV40-scFv-GCN4-sfGFP-VPR-GB1-NLS To construct the VPR system, the sequences VP64 (PCR amplified from the Addgene plasmid, # 60904), Polymerase Chain Reaction:Article Title: MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo Article Snippet: .. Plasmid Construction 1) pHRdSV40-scFv-GCN4-sfGFP-VPR-GB1-NLS To construct the VPR system, the sequences VP64 (PCR amplified from the Addgene plasmid, # 60904), Synthesized:Article Title: MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo Article Snippet: .. Plasmid Construction 1) pHRdSV40-scFv-GCN4-sfGFP-VPR-GB1-NLS To construct the VPR system, the sequences VP64 (PCR amplified from the Addgene plasmid, # 60904), Clone Assay:Article Title: MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo Article Snippet: .. Plasmid Construction 1) pHRdSV40-scFv-GCN4-sfGFP-VPR-GB1-NLS To construct the VPR system, the sequences VP64 (PCR amplified from the Addgene plasmid, # 60904), Sequencing:Article Title: MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo Article Snippet: .. Plasmid Construction 1) pHRdSV40-scFv-GCN4-sfGFP-VPR-GB1-NLS To construct the VPR system, the sequences VP64 (PCR amplified from the Addgene plasmid, # 60904), |
![( A ) KEGG enrichment analysis of down-regulated DEGs in Sik2 F/F ; LysM Cre cardiac macrophages on day 3 post-MI. The top 20 enriched pathways are listed. ( B ) DEGs (fold change ≥ 2 and P .adjust ≤ 0.05) overlapped in top 20 of enriched pathways. ( C ) Transcription factor prediction was performed using TRRUST databases. The top 10 transcription factors are listed. ( D ) Immunoblotting analysis and quantification of <t>P65</t> in the cytoplasm and nucleus of WT and SIK2-knockout PMs and treated with TNF-α (20 ng/ml) or vehicle ( n = 4) for 1 hour. ( E ) qPCR analysis of Il-1β , Il-6 , Nos2 , Cxcl-1 , and Mmp-9 mRNA expression in WT and SIK2-knockout PMs stimulated with TNF-α for 24 hours ( n = 6). ( F ) qPCR analysis of Il-1β , Il-6 , Nos2 , Cxcl-1 , and Mmp-9 mRNA expression ( n = 6). PMs were pretreated with ARN3236 (3 μmol) and stimulated with TNF-α for 24 hours. ( G ) WikiPathways enrichment analysis of differentially phosphorylated proteins in PMs treated with ARN3236 (3 μmol) or control. The top 20 enriched pathways are listed. ( H ) Immunoblotting analysis and quantification of phosphorylated P65 (p-P65), P65, phosphorylated P38 (p-P38), and P38 expression in WT and SIK2-knockout PMs stimulated with TNF-α or vehicle ( n = 6). ( I and J ) Flow cytometry analysis of efferocytosis in WT or LeSik2 Raw264.7 cells [stimulated with SB 203580 (10 μmol) or not] treated with CFSE-prelabeled RBCs ( n = 6).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4512/pmc13674512/pmc13674512__sciadv.adw6624-f5.jpg)
