p65 Search Results


anti nf κb p65ps529 pe  (Miltenyi Biotec)
94
Miltenyi Biotec anti nf κb p65ps529 pe
Anti Nf κb P65ps529 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p65/10__3892_slash_mco__2026__2939-69-24-29?v=Miltenyi+Biotec
Average 94 stars, based on 1 article reviews
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nuclear factor kappa β  (Elabscience Biotechnology)
95
Elabscience Biotechnology nuclear factor kappa β
Nuclear Factor Kappa β, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p65/10__4103_slash_jasmr__jasmr_11_23-42-22-32?v=Elabscience+Biotechnology
Average 95 stars, based on 1 article reviews
nuclear factor kappa β - by Bioz Stars, 2026-08
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nf κb primary antibody  (Novus Biologicals)
93
Novus Biologicals nf κb primary antibody
Nf κb Primary Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
nf κb primary antibody - by Bioz Stars, 2026-08
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c terminal ddk  (OriGene)
90
OriGene c terminal ddk
C Terminal Ddk, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p65/pmc03247947-49-9-20?v=OriGene
Average 90 stars, based on 1 article reviews
c terminal ddk - by Bioz Stars, 2026-08
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p iκbα ab176643  (Danaher Inc)
99
Danaher Inc p iκbα ab176643
P Iκbα Ab176643, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p65/pmc04954850-66-16-18?v=Danaher+Inc
Average 99 stars, based on 1 article reviews
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anti nf κb antibody  (Danaher Inc)
96
Danaher Inc anti nf κb antibody
Anti Nf κb Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p65/10__1039_slash_C9FO00583H-49-1-3?v=Danaher+Inc
Average 96 stars, based on 1 article reviews
anti nf κb antibody - by Bioz Stars, 2026-08
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transam nf κb p65 assay kit  (Active Motif)
98
Active Motif transam nf κb p65 assay kit
Transam Nf κb P65 Assay Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
transam nf κb p65 assay kit - by Bioz Stars, 2026-08
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control gfp shrna vectors  (Addgene inc)
92
Addgene inc control gfp shrna vectors
a–c Hypothalamic GnRH mRNA of indicated mice. d–g . GT1–7 cells were transfected with CA IKKβ, RelA or DN IκBα vs. control (Con) plasmid (d,e,g) , co-transfected with GnRH -promoter luciferase plasmid (e&f) , or together with RelA <t>shRNA</t> (sh-RelA) vs. control shRNA (sh-Con) plasmid (f) , and were measure for GnRH release (d) , GnRH promoter (e&f) , and c-Fos , c-Jun , PKCα and PKCδ mRNA levels (g). h . GnRH promoter activities were measured for GT1–7 cells transfected with GnRH -promoter luciferase plasmid, co-transfected with c-Jun or c-Fos plasmid vs. control plasmid (Con), or treated with TPA vs. vehicle (Veh). i . GnRH promoter activities were measured for GT1–7 cells transfected with GnRH -promoter luciferase plasmid, co-transfected with CA IKKβ vs. control (Con) plasmid, and with c-Fos /c- Jun shRNA plasmids (sh-c-Fos/sh-c-Jun) vs. scramble shRNA control (sh-Con). j . Summarized schematic model. *P < 0.05, **P < 0.01, ***P < 0.001; n = 12 (a&e) and 3 (f–i) per group, and n = 6 (b) , 8 (c) and 4 (d) in Con, n = 8 (b) and 6 (d) in IKKβ, and n = 8 (c) and 6 (d) in IκBα. Error bars reflect mean ± SEM.
Control Gfp Shrna Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p65/pmc03756938-178-3-10?v=Addgene+inc
Average 92 stars, based on 1 article reviews
control gfp shrna vectors - by Bioz Stars, 2026-08
92/100 stars
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p65  (Addgene inc)
92
Addgene inc p65
a–c Hypothalamic GnRH mRNA of indicated mice. d–g . GT1–7 cells were transfected with CA IKKβ, RelA or DN IκBα vs. control (Con) plasmid (d,e,g) , co-transfected with GnRH -promoter luciferase plasmid (e&f) , or together with RelA <t>shRNA</t> (sh-RelA) vs. control shRNA (sh-Con) plasmid (f) , and were measure for GnRH release (d) , GnRH promoter (e&f) , and c-Fos , c-Jun , PKCα and PKCδ mRNA levels (g). h . GnRH promoter activities were measured for GT1–7 cells transfected with GnRH -promoter luciferase plasmid, co-transfected with c-Jun or c-Fos plasmid vs. control plasmid (Con), or treated with TPA vs. vehicle (Veh). i . GnRH promoter activities were measured for GT1–7 cells transfected with GnRH -promoter luciferase plasmid, co-transfected with CA IKKβ vs. control (Con) plasmid, and with c-Fos /c- Jun shRNA plasmids (sh-c-Fos/sh-c-Jun) vs. scramble shRNA control (sh-Con). j . Summarized schematic model. *P < 0.05, **P < 0.01, ***P < 0.001; n = 12 (a&e) and 3 (f–i) per group, and n = 6 (b) , 8 (c) and 4 (d) in Con, n = 8 (b) and 6 (d) in IKKβ, and n = 8 (c) and 6 (d) in IκBα. Error bars reflect mean ± SEM.
P65, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p65/pmc07598068-602-14-11?v=Addgene+inc
Average 92 stars, based on 1 article reviews
p65 - by Bioz Stars, 2026-08
92/100 stars
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lentims2 p65 hsf1 hygro  (Addgene inc)
95
Addgene inc lentims2 p65 hsf1 hygro
a–c Hypothalamic GnRH mRNA of indicated mice. d–g . GT1–7 cells were transfected with CA IKKβ, RelA or DN IκBα vs. control (Con) plasmid (d,e,g) , co-transfected with GnRH -promoter luciferase plasmid (e&f) , or together with RelA <t>shRNA</t> (sh-RelA) vs. control shRNA (sh-Con) plasmid (f) , and were measure for GnRH release (d) , GnRH promoter (e&f) , and c-Fos , c-Jun , PKCα and PKCδ mRNA levels (g). h . GnRH promoter activities were measured for GT1–7 cells transfected with GnRH -promoter luciferase plasmid, co-transfected with c-Jun or c-Fos plasmid vs. control plasmid (Con), or treated with TPA vs. vehicle (Veh). i . GnRH promoter activities were measured for GT1–7 cells transfected with GnRH -promoter luciferase plasmid, co-transfected with CA IKKβ vs. control (Con) plasmid, and with c-Fos /c- Jun shRNA plasmids (sh-c-Fos/sh-c-Jun) vs. scramble shRNA control (sh-Con). j . Summarized schematic model. *P < 0.05, **P < 0.01, ***P < 0.001; n = 12 (a&e) and 3 (f–i) per group, and n = 6 (b) , 8 (c) and 4 (d) in Con, n = 8 (b) and 6 (d) in IKKβ, and n = 8 (c) and 6 (d) in IκBα. Error bars reflect mean ± SEM.
Lentims2 P65 Hsf1 Hygro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p65/pmc09636131-327-0-9?v=Addgene+inc
Average 95 stars, based on 1 article reviews
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phospho ser536 nf b p65  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phospho ser536 nf b p65
FIG. 2. PKC does not regulate NFB signaling in A549 cells. A, immunoblot analysis of A549 cell transfectants for cIAP2, Bcl-XL, and actin. B, analysis of A549 cell transfectants for PARP and cleaved PARP. HeLa cells treated with taxol for either 24 or 48 h served as a positive control for cleaved PARP. C, A549/pBabe and A549/kdPKC cells were treated with 50 g/ml TNF for the indicated times and cell lysates subjected to immunoblot analysis for detection of IB, phospho-(Ser32)-IB, and actin. D, A549/pBabe and A549/kdPKC cells were treated with 50 g/ml TNF for the indicated times and cell lysates subjected to immunoblot analysis for detection of NFB <t>p65,</t> phospho-(Ser536)-NFB, p65, and actin. E, transcriptional activity of an NFB-luciferase reporter in A549/pBabe and A549/kdPKC cells in the presence and absence of TNF. Data represent the mean S.E. of three independent determinations.
Phospho Ser536 Nf B P65, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p65/10__1074_slash_jbc__m505402200-48-111-115?v=Cell+Signaling+Technology+Inc
Average 96 stars, based on 1 article reviews
phospho ser536 nf b p65 - by Bioz Stars, 2026-08
96/100 stars
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nf κb  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc nf κb
FIG. 2. PKC does not regulate NFB signaling in A549 cells. A, immunoblot analysis of A549 cell transfectants for cIAP2, Bcl-XL, and actin. B, analysis of A549 cell transfectants for PARP and cleaved PARP. HeLa cells treated with taxol for either 24 or 48 h served as a positive control for cleaved PARP. C, A549/pBabe and A549/kdPKC cells were treated with 50 g/ml TNF for the indicated times and cell lysates subjected to immunoblot analysis for detection of IB, phospho-(Ser32)-IB, and actin. D, A549/pBabe and A549/kdPKC cells were treated with 50 g/ml TNF for the indicated times and cell lysates subjected to immunoblot analysis for detection of NFB <t>p65,</t> phospho-(Ser536)-NFB, p65, and actin. E, transcriptional activity of an NFB-luciferase reporter in A549/pBabe and A549/kdPKC cells in the presence and absence of TNF. Data represent the mean S.E. of three independent determinations.
Nf κb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p65/pm34454753-85-21-24?v=Cell+Signaling+Technology+Inc
Average 96 stars, based on 1 article reviews
nf κb - by Bioz Stars, 2026-08
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Image Search Results


a–c Hypothalamic GnRH mRNA of indicated mice. d–g . GT1–7 cells were transfected with CA IKKβ, RelA or DN IκBα vs. control (Con) plasmid (d,e,g) , co-transfected with GnRH -promoter luciferase plasmid (e&f) , or together with RelA shRNA (sh-RelA) vs. control shRNA (sh-Con) plasmid (f) , and were measure for GnRH release (d) , GnRH promoter (e&f) , and c-Fos , c-Jun , PKCα and PKCδ mRNA levels (g). h . GnRH promoter activities were measured for GT1–7 cells transfected with GnRH -promoter luciferase plasmid, co-transfected with c-Jun or c-Fos plasmid vs. control plasmid (Con), or treated with TPA vs. vehicle (Veh). i . GnRH promoter activities were measured for GT1–7 cells transfected with GnRH -promoter luciferase plasmid, co-transfected with CA IKKβ vs. control (Con) plasmid, and with c-Fos /c- Jun shRNA plasmids (sh-c-Fos/sh-c-Jun) vs. scramble shRNA control (sh-Con). j . Summarized schematic model. *P < 0.05, **P < 0.01, ***P < 0.001; n = 12 (a&e) and 3 (f–i) per group, and n = 6 (b) , 8 (c) and 4 (d) in Con, n = 8 (b) and 6 (d) in IKKβ, and n = 8 (c) and 6 (d) in IκBα. Error bars reflect mean ± SEM.

Journal: Nature

Article Title: Hypothalamic Programming of Systemic Aging Involving IKKβ/NF-κB and GnRH

doi: 10.1038/nature12143

Figure Lengend Snippet: a–c Hypothalamic GnRH mRNA of indicated mice. d–g . GT1–7 cells were transfected with CA IKKβ, RelA or DN IκBα vs. control (Con) plasmid (d,e,g) , co-transfected with GnRH -promoter luciferase plasmid (e&f) , or together with RelA shRNA (sh-RelA) vs. control shRNA (sh-Con) plasmid (f) , and were measure for GnRH release (d) , GnRH promoter (e&f) , and c-Fos , c-Jun , PKCα and PKCδ mRNA levels (g). h . GnRH promoter activities were measured for GT1–7 cells transfected with GnRH -promoter luciferase plasmid, co-transfected with c-Jun or c-Fos plasmid vs. control plasmid (Con), or treated with TPA vs. vehicle (Veh). i . GnRH promoter activities were measured for GT1–7 cells transfected with GnRH -promoter luciferase plasmid, co-transfected with CA IKKβ vs. control (Con) plasmid, and with c-Fos /c- Jun shRNA plasmids (sh-c-Fos/sh-c-Jun) vs. scramble shRNA control (sh-Con). j . Summarized schematic model. *P < 0.05, **P < 0.01, ***P < 0.001; n = 12 (a&e) and 3 (f–i) per group, and n = 6 (b) , 8 (c) and 4 (d) in Con, n = 8 (b) and 6 (d) in IKKβ, and n = 8 (c) and 6 (d) in IκBα. Error bars reflect mean ± SEM.

Article Snippet: RelA shRNA and control (GFP) shRNA vectors were obtained from Addgene (# 22507, 22508, and 31848), as studied in the literature (RelA shRNA: GCA-TGC-GAT-TCC-GCT-ATA-A, control shRNA: ACA-GCC-ACA-ACG-TCT-ATA-T).

Techniques: Transfection, Control, Plasmid Preparation, Luciferase, shRNA

FIG. 2. PKC does not regulate NFB signaling in A549 cells. A, immunoblot analysis of A549 cell transfectants for cIAP2, Bcl-XL, and actin. B, analysis of A549 cell transfectants for PARP and cleaved PARP. HeLa cells treated with taxol for either 24 or 48 h served as a positive control for cleaved PARP. C, A549/pBabe and A549/kdPKC cells were treated with 50 g/ml TNF for the indicated times and cell lysates subjected to immunoblot analysis for detection of IB, phospho-(Ser32)-IB, and actin. D, A549/pBabe and A549/kdPKC cells were treated with 50 g/ml TNF for the indicated times and cell lysates subjected to immunoblot analysis for detection of NFB p65, phospho-(Ser536)-NFB, p65, and actin. E, transcriptional activity of an NFB-luciferase reporter in A549/pBabe and A549/kdPKC cells in the presence and absence of TNF. Data represent the mean S.E. of three independent determinations.

Journal: Journal of Biological Chemistry

Article Title: Atypical Protein Kinase Cι Plays a Critical Role in Human Lung Cancer Cell Growth and Tumorigenicity

doi: 10.1074/jbc.m505402200

Figure Lengend Snippet: FIG. 2. PKC does not regulate NFB signaling in A549 cells. A, immunoblot analysis of A549 cell transfectants for cIAP2, Bcl-XL, and actin. B, analysis of A549 cell transfectants for PARP and cleaved PARP. HeLa cells treated with taxol for either 24 or 48 h served as a positive control for cleaved PARP. C, A549/pBabe and A549/kdPKC cells were treated with 50 g/ml TNF for the indicated times and cell lysates subjected to immunoblot analysis for detection of IB, phospho-(Ser32)-IB, and actin. D, A549/pBabe and A549/kdPKC cells were treated with 50 g/ml TNF for the indicated times and cell lysates subjected to immunoblot analysis for detection of NFB p65, phospho-(Ser536)-NFB, p65, and actin. E, transcriptional activity of an NFB-luciferase reporter in A549/pBabe and A549/kdPKC cells in the presence and absence of TNF. Data represent the mean S.E. of three independent determinations.

Article Snippet: This paper is available on line at http://www.jbc.org 31109 at K U N G L IG A T E K N ISK A H O G SK O L A N on M arch 8, 2015 http://w w w .jbc.org/ D ow nloaded from (Sigma, catalog number A8592); Rac1, 1:3000 (BD Transduction Laboratories, catalog number 610651); cIAP2, 1:500 (Santa Cruz Biotechnology, catalog number sc-7944); Bcl-XL, 1:1,000 (Cell Signaling Technologies, catalog number 2762); PARP/cleaved PARP, 1:1,000 (Cell Signaling Technologies, catalog number 9542); I B , 1:1,000 (Cell Signaling Technologies, catalog number 9242); phospho-(Ser32)-I B , 1:1,000 (Cell Signaling Technologies, catalog number 9241); NF B p65, 1:1,000 (Cell Signaling Technologies, catalog number 3034); phospho(Ser536)-NF B p65, 1:1,000 (Cell Signaling Technologies, catalog number 3031); Mek, phospho-(Ser217/221)-Mek, and phospho-(Ser298)Mek, each at 1:1,000 (Cell Signaling Technologies, catalog numbers 9122, 9121, and 9128, respectively), Erk and phospho-(Thr202/Tyr204)Erk, each at 1:1,000 (Cell Signaling Technologies, catalog numbers 9102 and 9101, respectively); CD31, 1:1,000 (or Pecam-1; Santa Cruz Biotechnology, catalog number sc-1506); BrdUrd, 1:100 (DAKO, catalog number M0744).

Techniques: Western Blot, Positive Control, Activity Assay, Luciferase