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10355 1 ap  (Proteintech)


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    Structured Review

    Proteintech 10355 1 ap
    The information on the antibody.
    10355 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1421 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p21/P21+Antibody/pmc13621234-1-4-8
    Average 96 stars, based on 1421 article reviews
    10355 1 ap - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Ghrelin Alleviates Aging‐Related Cognitive Impairment by Regulating Autophagy‐Related Signaling via the SHBG / JNK1 /Beclin1 Axis in Microglia"

    Article Title: Ghrelin Alleviates Aging‐Related Cognitive Impairment by Regulating Autophagy‐Related Signaling via the SHBG / JNK1 /Beclin1 Axis in Microglia

    Journal: Aging Cell

    doi: 10.1111/acel.70732

    The information on the antibody.
    Figure Legend Snippet: The information on the antibody.

    Techniques Used:

    Related Articles

    Blocking Assay:

    Article Title: A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer
    Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, LG18-1501M − 1H). .. After blocking with 5 % nonfat milk for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies, including AMPK (1:1000, CST, 2532), p-AMPK (1:1000, CST, 2535), CPT1B (1:1000, Proteintech, 22170-1-AP), CDK4 (1:1000, Proteintech, 11026-1-AP), PCNA (1:1000, Proteintech, 10205-2-AP), p21 (1:1000, Proteintech, 10355-1-AP), GAPDH (1:1000, Proteintech, 60004-1-Ig) followed by HRP-conjugated secondary antibody (1:5000, Proteintech, RGAR001) for 1 h at room temperature. ..

    Incubation:

    Article Title: A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer
    Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, LG18-1501M − 1H). .. After blocking with 5 % nonfat milk for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies, including AMPK (1:1000, CST, 2532), p-AMPK (1:1000, CST, 2535), CPT1B (1:1000, Proteintech, 22170-1-AP), CDK4 (1:1000, Proteintech, 11026-1-AP), PCNA (1:1000, Proteintech, 10205-2-AP), p21 (1:1000, Proteintech, 10355-1-AP), GAPDH (1:1000, Proteintech, 60004-1-Ig) followed by HRP-conjugated secondary antibody (1:5000, Proteintech, RGAR001) for 1 h at room temperature. ..

    Article Title: Microenvironment-educated MSC-EVs loaded injectable smart hydrogel for targeting senescent nucleus pulposus cells and inhibiting ferroptosis against intervertebral disc degeneration
    Article Snippet: .. After blocked with 5% non-fat milk for 2 h at room temperature, the membranes were incubated with primary antibodies against GAPDH (1:5000, 104941-AP, Proteintech), TSG101 (1:1000, DF8427, Affinity), CD9 (1:1000, AF5139, Affinity), CD63 (1:2000, 25682-1-AP, Proteintech), Calnexin (1:5000, 10427-2-AP, Proteintech), GM130 (1:20000, 11308-1-AP, Proteintech), CXCR3 (1:5000, 26756-1-AP, Proteintech), CXCL10 (1:2000, 10937-1-AP, Proteintech), MMP3 (1:2000, 17873-1-AP, Proteintech), ADAMTS5 (DF13268, Affinity), P16 (AF5484, Affinity), P21 (10355-1-AP, Proteintech), GPX4 (1:1000, 381958, Zen-bio), SLC7A11 (1:1000, 26864-1-AP, Proteintech), ACSL4 (1:5000, 22401-1-AP, Proteintech) and Tubulin (1:10000, T40103 , Abmart) overnight at 4 °C. .. After washing three times with TBST (Tris-buffered saline and 0.1% Tween 20), the membranes were incubated with secondary antibodies (1:5000, SA00001-2, SA00001-1, Proteintech) for 2 h at room temperature.

    Article Title: FOXP1 deficiency exacerbates cellular senescence in PM 2.5 -induced renal injury.
    Article Snippet: .. The protein samples were subjected to heat-induced denaturation, and was run on an SDS-PAGE gel, transferred to PVDF membranes (Millipore, IPVH00010), thenblocked with 5% BSA and incubated at 4 °C overnight with FOXP1 (1:1000, ab314488, Abcam, UK), P21 (1:1000, AWA63269, Abiowell, China), Klotho (1:1000, 28100–1-AP, Proteintech, USA), P16 (1:500, R23895, ZenBio, China), 4-HNE(1:1000, ab48506, Abcam, UK), PCNA(1:1000, 50616, ProMab Biotechnologies, Inc., China) andγH2AX (1:2000, BM4841, Boster, China) primary antibodies. .. Subsequently, secondary antibodies were added to the membranes and allowed to react at ambient temperature for one hour, followed by visualization using the enhanced chemiluminescence and the detection system (Amersham ImageQuant 800, Cytiva, Marlborough, USA), finaly quantified by ImageJ software.

    Article Title: Magnesium Deficiency Accelerates Gut Aging and Increases Susceptibility to Colitis.
    Article Snippet: .. Membranes were blocked with 5% nonfat dry milk in PBST (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 0.1% (w/v) Tween 20 detergent) and incubated overnight at 4°C with primary antibodies against CTNNA1 (HuaBio, ER62912; 1:1000), CTNNB1 (HuaBio, ET1601- 5; 1:1000), CTNND1 (HuaBio, ER1803- 71; 1:1000), CLDN1 (HuaBio, ER1906- 37; 1:1000), P21 (Proteintech, 10355- 1- AP; 1:1000), TJP2 (Proteintech, 18900- 1- AP; 1:1000), JAM1 (HuaBio, ET1610- 90; 1:1000) and GAPDH (Proteintech, 60004- 1- Ig; 1:5000). .. Following three PBST washes, membranes were incubated with appropriate HRPconjugated secondary antibodies at room temperature for 1 h. Protein bands were visualized using Immobilon Western HRP Substrate (Millipore) or an equivalent chemiluminescent detection system.

    other:

    Article Title: cGAS-STING/HMGB1-mediated senescence induced by LRRK2 accelerates cartilage degeneration in osteoarthritis.
    Article Snippet: LRRK2 antibody (#A17253) was obtained from Abclonal Antibody (Wuhan, China).

    Article Title: cGAS-STING/HMGB1-mediated senescence induced by LRRK2 accelerates cartilage degeneration in osteoarthritis
    Article Snippet: Anti-HMGB1 (#CPA7037) was obtained from Cohesion Biosciences (London, UK).

    SDS Page:

    Article Title: FOXP1 deficiency exacerbates cellular senescence in PM 2.5 -induced renal injury.
    Article Snippet: .. The protein samples were subjected to heat-induced denaturation, and was run on an SDS-PAGE gel, transferred to PVDF membranes (Millipore, IPVH00010), thenblocked with 5% BSA and incubated at 4 °C overnight with FOXP1 (1:1000, ab314488, Abcam, UK), P21 (1:1000, AWA63269, Abiowell, China), Klotho (1:1000, 28100–1-AP, Proteintech, USA), P16 (1:500, R23895, ZenBio, China), 4-HNE(1:1000, ab48506, Abcam, UK), PCNA(1:1000, 50616, ProMab Biotechnologies, Inc., China) andγH2AX (1:2000, BM4841, Boster, China) primary antibodies. .. Subsequently, secondary antibodies were added to the membranes and allowed to react at ambient temperature for one hour, followed by visualization using the enhanced chemiluminescence and the detection system (Amersham ImageQuant 800, Cytiva, Marlborough, USA), finaly quantified by ImageJ software.



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    Neutrophils from aged mice display enhanced inflammatory and effector‐associated programs. (A) t‐SNE plot of the integrated neutrophil atlas from multiple organs, including blood, intestine, liver, lung, vascular tissue, heart, kidney, and brain, colored by age group. The number of neutrophils contributed by each group is shown in the accompanying bar plot. (B) Volcano plot showing differential gene expression in neutrophils from aged versus young mice. Genes with adjusted p value < 0.05 and log2 Fold Change > 0.5 were designated as differentially expressed genes. (C, D) Hallmark functional enrichment analysis of gene sets enriched in neutrophils from aged (C) or young (D) samples. Dot size represents gene count and color indicates adjusted significance. (E) Sample‐level comparison of marrow proximity and reactive oxygen species (ROS) module scores in neutrophils from young and aged mice. Each dot represents one biological sample. (F) Representative Cytox Green staining images showing NET formation in neutrophils isolated from young and aged mice. Quantification of NET‐positive cells is shown on the right, each data point represents one biological replicate. Scale bar, 50 μm. (G) Representative senescence‐associated β‐galactosidase (β‐Gal) staining images of MOVAS and AML‐12 cells following treatment with conditional medium derived from young or aged neutrophils. Quantification of β‐Gal‐positive cells is shown on the right, each data point represents one biological replicate. Scale bar, 20 μm. (H, I) Immunoblot (H) and its quantification analysis (I) of MMP12, MMP9, P16, and <t>P21</t> expression in MOVAS and AML‐12 cells treated with conditioned medium from young or aged neutrophils. GAPDH served as a loading control. Relative protein intensities normalized to young group are indicated below each blot. Each plot represents an independent biological replicate. (J) Relative RNA expression of senescence‐associated secretory phenotype (SASP) genes ( Il6 , Tnf , and Ifn ) and extracellular matrix remodeling genes ( Mmp3 and Mmp9 ) in MOVAS cells following treatment with conditioned medium from young or aged neutrophils. Data are presented as mean ± s.e.m. Statistical significance was determined using two‐sided unpaired t ‐test (E–G, I) and two‐way ANOVA followed by Tukey's multiple‐comparisons test (J). * p < 0.05 ; ** p < 0.01; *** p < 0.001.
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    Neutrophils from aged mice display enhanced inflammatory and effector‐associated programs. (A) t‐SNE plot of the integrated neutrophil atlas from multiple organs, including blood, intestine, liver, lung, vascular tissue, heart, kidney, and brain, colored by age group. The number of neutrophils contributed by each group is shown in the accompanying bar plot. (B) Volcano plot showing differential gene expression in neutrophils from aged versus young mice. Genes with adjusted p value < 0.05 and log2 Fold Change > 0.5 were designated as differentially expressed genes. (C, D) Hallmark functional enrichment analysis of gene sets enriched in neutrophils from aged (C) or young (D) samples. Dot size represents gene count and color indicates adjusted significance. (E) Sample‐level comparison of marrow proximity and reactive oxygen species (ROS) module scores in neutrophils from young and aged mice. Each dot represents one biological sample. (F) Representative Cytox Green staining images showing NET formation in neutrophils isolated from young and aged mice. Quantification of NET‐positive cells is shown on the right, each data point represents one biological replicate. Scale bar, 50 μm. (G) Representative senescence‐associated β‐galactosidase (β‐Gal) staining images of MOVAS and AML‐12 cells following treatment with conditional medium derived from young or aged neutrophils. Quantification of β‐Gal‐positive cells is shown on the right, each data point represents one biological replicate. Scale bar, 20 μm. (H, I) Immunoblot (H) and its quantification analysis (I) of MMP12, MMP9, P16, and <t>P21</t> expression in MOVAS and AML‐12 cells treated with conditioned medium from young or aged neutrophils. GAPDH served as a loading control. Relative protein intensities normalized to young group are indicated below each blot. Each plot represents an independent biological replicate. (J) Relative RNA expression of senescence‐associated secretory phenotype (SASP) genes ( Il6 , Tnf , and Ifn ) and extracellular matrix remodeling genes ( Mmp3 and Mmp9 ) in MOVAS cells following treatment with conditioned medium from young or aged neutrophils. Data are presented as mean ± s.e.m. Statistical significance was determined using two‐sided unpaired t ‐test (E–G, I) and two‐way ANOVA followed by Tukey's multiple‐comparisons test (J). * p < 0.05 ; ** p < 0.01; *** p < 0.001.
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    Neutrophils from aged mice display enhanced inflammatory and effector‐associated programs. (A) t‐SNE plot of the integrated neutrophil atlas from multiple organs, including blood, intestine, liver, lung, vascular tissue, heart, kidney, and brain, colored by age group. The number of neutrophils contributed by each group is shown in the accompanying bar plot. (B) Volcano plot showing differential gene expression in neutrophils from aged versus young mice. Genes with adjusted p value < 0.05 and log2 Fold Change > 0.5 were designated as differentially expressed genes. (C, D) Hallmark functional enrichment analysis of gene sets enriched in neutrophils from aged (C) or young (D) samples. Dot size represents gene count and color indicates adjusted significance. (E) Sample‐level comparison of marrow proximity and reactive oxygen species (ROS) module scores in neutrophils from young and aged mice. Each dot represents one biological sample. (F) Representative Cytox Green staining images showing NET formation in neutrophils isolated from young and aged mice. Quantification of NET‐positive cells is shown on the right, each data point represents one biological replicate. Scale bar, 50 μm. (G) Representative senescence‐associated β‐galactosidase (β‐Gal) staining images of MOVAS and AML‐12 cells following treatment with conditional medium derived from young or aged neutrophils. Quantification of β‐Gal‐positive cells is shown on the right, each data point represents one biological replicate. Scale bar, 20 μm. (H, I) Immunoblot (H) and its quantification analysis (I) of MMP12, MMP9, P16, and <t>P21</t> expression in MOVAS and AML‐12 cells treated with conditioned medium from young or aged neutrophils. GAPDH served as a loading control. Relative protein intensities normalized to young group are indicated below each blot. Each plot represents an independent biological replicate. (J) Relative RNA expression of senescence‐associated secretory phenotype (SASP) genes ( Il6 , Tnf , and Ifn ) and extracellular matrix remodeling genes ( Mmp3 and Mmp9 ) in MOVAS cells following treatment with conditioned medium from young or aged neutrophils. Data are presented as mean ± s.e.m. Statistical significance was determined using two‐sided unpaired t ‐test (E–G, I) and two‐way ANOVA followed by Tukey's multiple‐comparisons test (J). * p < 0.05 ; ** p < 0.01; *** p < 0.001.
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    Neutrophils from aged mice display enhanced inflammatory and effector‐associated programs. (A) t‐SNE plot of the integrated neutrophil atlas from multiple organs, including blood, intestine, liver, lung, vascular tissue, heart, kidney, and brain, colored by age group. The number of neutrophils contributed by each group is shown in the accompanying bar plot. (B) Volcano plot showing differential gene expression in neutrophils from aged versus young mice. Genes with adjusted p value < 0.05 and log2 Fold Change > 0.5 were designated as differentially expressed genes. (C, D) Hallmark functional enrichment analysis of gene sets enriched in neutrophils from aged (C) or young (D) samples. Dot size represents gene count and color indicates adjusted significance. (E) Sample‐level comparison of marrow proximity and reactive oxygen species (ROS) module scores in neutrophils from young and aged mice. Each dot represents one biological sample. (F) Representative Cytox Green staining images showing NET formation in neutrophils isolated from young and aged mice. Quantification of NET‐positive cells is shown on the right, each data point represents one biological replicate. Scale bar, 50 μm. (G) Representative senescence‐associated β‐galactosidase (β‐Gal) staining images of MOVAS and AML‐12 cells following treatment with conditional medium derived from young or aged neutrophils. Quantification of β‐Gal‐positive cells is shown on the right, each data point represents one biological replicate. Scale bar, 20 μm. (H, I) Immunoblot (H) and its quantification analysis (I) of MMP12, MMP9, P16, and <t>P21</t> expression in MOVAS and AML‐12 cells treated with conditioned medium from young or aged neutrophils. GAPDH served as a loading control. Relative protein intensities normalized to young group are indicated below each blot. Each plot represents an independent biological replicate. (J) Relative RNA expression of senescence‐associated secretory phenotype (SASP) genes ( Il6 , Tnf , and Ifn ) and extracellular matrix remodeling genes ( Mmp3 and Mmp9 ) in MOVAS cells following treatment with conditioned medium from young or aged neutrophils. Data are presented as mean ± s.e.m. Statistical significance was determined using two‐sided unpaired t ‐test (E–G, I) and two‐way ANOVA followed by Tukey's multiple‐comparisons test (J). * p < 0.05 ; ** p < 0.01; *** p < 0.001.
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    Image Search Results


    The information on the antibody.

    Journal: Aging Cell

    Article Title: Ghrelin Alleviates Aging‐Related Cognitive Impairment by Regulating Autophagy‐Related Signaling via the SHBG / JNK1 /Beclin1 Axis in Microglia

    doi: 10.1111/acel.70732

    Figure Lengend Snippet: The information on the antibody.

    Article Snippet: p21 , 1:2000 , 10355‐1‐AP , Rabbit , Proteintech , USA.

    Techniques:

    Neutrophils from aged mice display enhanced inflammatory and effector‐associated programs. (A) t‐SNE plot of the integrated neutrophil atlas from multiple organs, including blood, intestine, liver, lung, vascular tissue, heart, kidney, and brain, colored by age group. The number of neutrophils contributed by each group is shown in the accompanying bar plot. (B) Volcano plot showing differential gene expression in neutrophils from aged versus young mice. Genes with adjusted p value < 0.05 and log2 Fold Change > 0.5 were designated as differentially expressed genes. (C, D) Hallmark functional enrichment analysis of gene sets enriched in neutrophils from aged (C) or young (D) samples. Dot size represents gene count and color indicates adjusted significance. (E) Sample‐level comparison of marrow proximity and reactive oxygen species (ROS) module scores in neutrophils from young and aged mice. Each dot represents one biological sample. (F) Representative Cytox Green staining images showing NET formation in neutrophils isolated from young and aged mice. Quantification of NET‐positive cells is shown on the right, each data point represents one biological replicate. Scale bar, 50 μm. (G) Representative senescence‐associated β‐galactosidase (β‐Gal) staining images of MOVAS and AML‐12 cells following treatment with conditional medium derived from young or aged neutrophils. Quantification of β‐Gal‐positive cells is shown on the right, each data point represents one biological replicate. Scale bar, 20 μm. (H, I) Immunoblot (H) and its quantification analysis (I) of MMP12, MMP9, P16, and P21 expression in MOVAS and AML‐12 cells treated with conditioned medium from young or aged neutrophils. GAPDH served as a loading control. Relative protein intensities normalized to young group are indicated below each blot. Each plot represents an independent biological replicate. (J) Relative RNA expression of senescence‐associated secretory phenotype (SASP) genes ( Il6 , Tnf , and Ifn ) and extracellular matrix remodeling genes ( Mmp3 and Mmp9 ) in MOVAS cells following treatment with conditioned medium from young or aged neutrophils. Data are presented as mean ± s.e.m. Statistical significance was determined using two‐sided unpaired t ‐test (E–G, I) and two‐way ANOVA followed by Tukey's multiple‐comparisons test (J). * p < 0.05 ; ** p < 0.01; *** p < 0.001.

    Journal: Aging Cell

    Article Title: Single‐Cell Mapping Identifies Aging‐Associated Neutrophil Remodeling Across Multiple Organs

    doi: 10.1111/acel.70735

    Figure Lengend Snippet: Neutrophils from aged mice display enhanced inflammatory and effector‐associated programs. (A) t‐SNE plot of the integrated neutrophil atlas from multiple organs, including blood, intestine, liver, lung, vascular tissue, heart, kidney, and brain, colored by age group. The number of neutrophils contributed by each group is shown in the accompanying bar plot. (B) Volcano plot showing differential gene expression in neutrophils from aged versus young mice. Genes with adjusted p value < 0.05 and log2 Fold Change > 0.5 were designated as differentially expressed genes. (C, D) Hallmark functional enrichment analysis of gene sets enriched in neutrophils from aged (C) or young (D) samples. Dot size represents gene count and color indicates adjusted significance. (E) Sample‐level comparison of marrow proximity and reactive oxygen species (ROS) module scores in neutrophils from young and aged mice. Each dot represents one biological sample. (F) Representative Cytox Green staining images showing NET formation in neutrophils isolated from young and aged mice. Quantification of NET‐positive cells is shown on the right, each data point represents one biological replicate. Scale bar, 50 μm. (G) Representative senescence‐associated β‐galactosidase (β‐Gal) staining images of MOVAS and AML‐12 cells following treatment with conditional medium derived from young or aged neutrophils. Quantification of β‐Gal‐positive cells is shown on the right, each data point represents one biological replicate. Scale bar, 20 μm. (H, I) Immunoblot (H) and its quantification analysis (I) of MMP12, MMP9, P16, and P21 expression in MOVAS and AML‐12 cells treated with conditioned medium from young or aged neutrophils. GAPDH served as a loading control. Relative protein intensities normalized to young group are indicated below each blot. Each plot represents an independent biological replicate. (J) Relative RNA expression of senescence‐associated secretory phenotype (SASP) genes ( Il6 , Tnf , and Ifn ) and extracellular matrix remodeling genes ( Mmp3 and Mmp9 ) in MOVAS cells following treatment with conditioned medium from young or aged neutrophils. Data are presented as mean ± s.e.m. Statistical significance was determined using two‐sided unpaired t ‐test (E–G, I) and two‐way ANOVA followed by Tukey's multiple‐comparisons test (J). * p < 0.05 ; ** p < 0.01; *** p < 0.001.

    Article Snippet: MMP2, MMP9, MMP12, P16, and GAPDH were purchased from Proteintech; P53 was purchased from Affinity; P21 was from Santa Cruz.

    Techniques: Tissue, Gene Expression, Functional Assay, Analysis, Comparison, Staining, Isolation, Derivative Assay, Western Blot, Expressing, Control, RNA Expression