pbs buffer  (Teknova)

Journal: iScience

Article Title: Schwann cells contribute to demyelinating diabetic neuropathy and nerve terminal structures in white adipose tissue

doi: 10.1016/j.isci.2023.106189

Figure Lengend Snippet: BTBR ob/ob mice present with a loss of small fiber sympathetic innervation in scWAT, accompanied by small fiber demyelination Male and female BTBR ob/ob (MUT, N = 4) and wild-type littermates (WT, N = 3). Western blot protein analysis of TH (A), SOX10 (B), and MPZ (C) in inguinal scWAT. A consistent nerve bundle entering scWAT depot was excised from each mouse, cross-sectioned, and stained with toluidine blue (D). G-ratio was measured for all myelinated axons in a nerve bundle and averaged for each mouse (E). G-ratios were pooled for each group (WT, 377; MUT, 981) and plotted against axon diameter (F). Linear regression values plotted in table below with slopes and Y-intercepts compared (F). Whole mount immunostaining of sympathetic nerves in axillary scWAT was captured as Z-stacks (10 μm step size) with a 10X objective, tiled 5 × 5 (22.85 mm 2 area), and maximum intensity projected (G). Neurite density measured as fluorescence area in each 22.85 mm 2 field of view (H). Whole mount immunostaining of inguinal scWAT nerves (TUBB3, green) and myelin (MPZ, red) demonstrating myelinated nerve bundles (I) and demyelination of small nerve fibers in MUT mice (J). Demyelination shown progressing along the length of comparable small fiber nerves overlying the subiliac lymph node (SiLN) in scWAT (K). Numbers 1–4 correspond to adjacent images digitally magnified to aid visualization (K). Brightfield images were captured on a Zeiss Axioskop microscope (D) and fluorescence images were captured on a Stellaris 5 confocal microscope (G,I–K). Lightning deconvolution (I–J). Statistics: unpaired Student’s t-test (A–C,E,H) and a linear regression (F). Error bars are SEMs. P-values are as shown. Scale bars from left to right: (D) 100 μm, 25 μm, (G) 1 mm, (I) 30.7 μm, (J) 13.5 μm, (K) 50 μm.

Article Snippet: 10X PBS Solution , Teknova , Cat#P0496.

Techniques: Western Blot, Staining, Immunostaining, Fluorescence, Microscopy

Journal: iScience

Article Title: Schwann cells contribute to demyelinating diabetic neuropathy and nerve terminal structures in white adipose tissue

doi: 10.1016/j.isci.2023.106189

Figure Lengend Snippet:

Article Snippet: 10X PBS Solution , Teknova , Cat#P0496.

Techniques: Recombinant, Electron Microscopy, Modification, SYBR Green Assay, RNA Extraction, Software, Real-time Polymerase Chain Reaction

Journal: STAR Protocols

Article Title: A clearing-free protocol for imaging intact whole adipose tissue innervation in mice

doi: 10.1016/j.xpro.2021.101109

Figure Lengend Snippet:

Article Snippet: 10× PBS Solution , Teknova , Cat# P0496.

Techniques: Recombinant, Electron Microscopy, Software, Generated, Microscopy