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ovcar3  (ATCC)


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    ATCC ovcar3
    The pre-targeting complex of NIR-PIT induces the release of ICD markers (A) Flow cytometric histograms show cell surface expression of calreticulin, HSP70, and HSP90 compared to untreated controls in SKOV3 cells with scFv-tisotumab-Zip2-Zip1-SNAP-IR700. (B) <t>OVCAR3</t> cells treated with scFv-Sacituzumab-Zip2-Zip1-SNAP-IR700. (C) IGROV1 cells treated with scFv-Farletuzumab-Zip2-Zip1-SNAP-IR700. Only viable cells were included in the analysis. Bar graphs show the mean fluorescence intensity (MFI) of cell surface calreticulin, HSP70, and HSP90. Data are represented as mean ± SD from three biological replicates. Cells exposed to NIR light irradiation without incubation with NIR-PIT agents were used as control. Statistical significance was determined by a one-way ANOVA and Dunnett's test. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ≤ 0.0001.
    Ovcar3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1534 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ovcar3/product/ATCC
    Average 99 stars, based on 1534 article reviews
    ovcar3 - by Bioz Stars, 2026-02
    99/100 stars

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    1) Product Images from "Synthetic zipper mediated pre-targeting system for near-infrared photoimmunotherapy"

    Article Title: Synthetic zipper mediated pre-targeting system for near-infrared photoimmunotherapy

    Journal: iScience

    doi: 10.1016/j.isci.2025.114558

    The pre-targeting complex of NIR-PIT induces the release of ICD markers (A) Flow cytometric histograms show cell surface expression of calreticulin, HSP70, and HSP90 compared to untreated controls in SKOV3 cells with scFv-tisotumab-Zip2-Zip1-SNAP-IR700. (B) OVCAR3 cells treated with scFv-Sacituzumab-Zip2-Zip1-SNAP-IR700. (C) IGROV1 cells treated with scFv-Farletuzumab-Zip2-Zip1-SNAP-IR700. Only viable cells were included in the analysis. Bar graphs show the mean fluorescence intensity (MFI) of cell surface calreticulin, HSP70, and HSP90. Data are represented as mean ± SD from three biological replicates. Cells exposed to NIR light irradiation without incubation with NIR-PIT agents were used as control. Statistical significance was determined by a one-way ANOVA and Dunnett's test. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ≤ 0.0001.
    Figure Legend Snippet: The pre-targeting complex of NIR-PIT induces the release of ICD markers (A) Flow cytometric histograms show cell surface expression of calreticulin, HSP70, and HSP90 compared to untreated controls in SKOV3 cells with scFv-tisotumab-Zip2-Zip1-SNAP-IR700. (B) OVCAR3 cells treated with scFv-Sacituzumab-Zip2-Zip1-SNAP-IR700. (C) IGROV1 cells treated with scFv-Farletuzumab-Zip2-Zip1-SNAP-IR700. Only viable cells were included in the analysis. Bar graphs show the mean fluorescence intensity (MFI) of cell surface calreticulin, HSP70, and HSP90. Data are represented as mean ± SD from three biological replicates. Cells exposed to NIR light irradiation without incubation with NIR-PIT agents were used as control. Statistical significance was determined by a one-way ANOVA and Dunnett's test. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ≤ 0.0001.

    Techniques Used: Expressing, Fluorescence, Irradiation, Incubation, Control



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    The pre-targeting complex of NIR-PIT induces the release of ICD markers (A) Flow cytometric histograms show cell surface expression of calreticulin, HSP70, and HSP90 compared to untreated controls in SKOV3 cells with scFv-tisotumab-Zip2-Zip1-SNAP-IR700. (B) <t>OVCAR3</t> cells treated with scFv-Sacituzumab-Zip2-Zip1-SNAP-IR700. (C) IGROV1 cells treated with scFv-Farletuzumab-Zip2-Zip1-SNAP-IR700. Only viable cells were included in the analysis. Bar graphs show the mean fluorescence intensity (MFI) of cell surface calreticulin, HSP70, and HSP90. Data are represented as mean ± SD from three biological replicates. Cells exposed to NIR light irradiation without incubation with NIR-PIT agents were used as control. Statistical significance was determined by a one-way ANOVA and Dunnett's test. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ≤ 0.0001.
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    The pre-targeting complex of NIR-PIT induces the release of ICD markers (A) Flow cytometric histograms show cell surface expression of calreticulin, HSP70, and HSP90 compared to untreated controls in SKOV3 cells with scFv-tisotumab-Zip2-Zip1-SNAP-IR700. (B) <t>OVCAR3</t> cells treated with scFv-Sacituzumab-Zip2-Zip1-SNAP-IR700. (C) IGROV1 cells treated with scFv-Farletuzumab-Zip2-Zip1-SNAP-IR700. Only viable cells were included in the analysis. Bar graphs show the mean fluorescence intensity (MFI) of cell surface calreticulin, HSP70, and HSP90. Data are represented as mean ± SD from three biological replicates. Cells exposed to NIR light irradiation without incubation with NIR-PIT agents were used as control. Statistical significance was determined by a one-way ANOVA and Dunnett's test. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ≤ 0.0001.
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    The pre-targeting complex of NIR-PIT induces the release of ICD markers (A) Flow cytometric histograms show cell surface expression of calreticulin, HSP70, and HSP90 compared to untreated controls in SKOV3 cells with scFv-tisotumab-Zip2-Zip1-SNAP-IR700. (B) <t>OVCAR3</t> cells treated with scFv-Sacituzumab-Zip2-Zip1-SNAP-IR700. (C) IGROV1 cells treated with scFv-Farletuzumab-Zip2-Zip1-SNAP-IR700. Only viable cells were included in the analysis. Bar graphs show the mean fluorescence intensity (MFI) of cell surface calreticulin, HSP70, and HSP90. Data are represented as mean ± SD from three biological replicates. Cells exposed to NIR light irradiation without incubation with NIR-PIT agents were used as control. Statistical significance was determined by a one-way ANOVA and Dunnett's test. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ≤ 0.0001.
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    The pre-targeting complex of NIR-PIT induces the release of ICD markers (A) Flow cytometric histograms show cell surface expression of calreticulin, HSP70, and HSP90 compared to untreated controls in SKOV3 cells with scFv-tisotumab-Zip2-Zip1-SNAP-IR700. (B) OVCAR3 cells treated with scFv-Sacituzumab-Zip2-Zip1-SNAP-IR700. (C) IGROV1 cells treated with scFv-Farletuzumab-Zip2-Zip1-SNAP-IR700. Only viable cells were included in the analysis. Bar graphs show the mean fluorescence intensity (MFI) of cell surface calreticulin, HSP70, and HSP90. Data are represented as mean ± SD from three biological replicates. Cells exposed to NIR light irradiation without incubation with NIR-PIT agents were used as control. Statistical significance was determined by a one-way ANOVA and Dunnett's test. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ≤ 0.0001.

    Journal: iScience

    Article Title: Synthetic zipper mediated pre-targeting system for near-infrared photoimmunotherapy

    doi: 10.1016/j.isci.2025.114558

    Figure Lengend Snippet: The pre-targeting complex of NIR-PIT induces the release of ICD markers (A) Flow cytometric histograms show cell surface expression of calreticulin, HSP70, and HSP90 compared to untreated controls in SKOV3 cells with scFv-tisotumab-Zip2-Zip1-SNAP-IR700. (B) OVCAR3 cells treated with scFv-Sacituzumab-Zip2-Zip1-SNAP-IR700. (C) IGROV1 cells treated with scFv-Farletuzumab-Zip2-Zip1-SNAP-IR700. Only viable cells were included in the analysis. Bar graphs show the mean fluorescence intensity (MFI) of cell surface calreticulin, HSP70, and HSP90. Data are represented as mean ± SD from three biological replicates. Cells exposed to NIR light irradiation without incubation with NIR-PIT agents were used as control. Statistical significance was determined by a one-way ANOVA and Dunnett's test. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ≤ 0.0001.

    Article Snippet: Ovarian cancer cell lines SKOV3 (HTB-77), OVCAR3 (HTB-161), IGROV1 (SCC-203), A2780 (ECACC-93112519), and HEK293T (CRL-11268) were obtained from the American Type Culture Collection, the European Collection of Authenticated Cell Cultures, and Sigma-Aldrich.

    Techniques: Expressing, Fluorescence, Irradiation, Incubation, Control