Journal: bioRxiv
Article Title: Abolishing respiratory complex I decreases in vivo growth of high grade serous ovarian cancer cells and sensitizes to anti-angiogenic therapy
doi: 10.64898/2026.02.28.708681
Figure Lengend Snippet: (a) HIF1a western blot analysis in representative OV-90 +/+ and OV90 −/− xenografts (n=3 per group). Calreticulin was used as loading control. (b) Gene expression of HIF1 targets evaluated by qRT-PCR in OV-90 +/+ (n=5) and OV-90 −/− (n=6) xenografts. Data were log transformed prior to statistical analysis. (c) Masson’s trichrome staining of OV-90 +/+ and OV90 −/− xenografts, with respective quantification of the stroma (collagen areas stained in blue). Representative images are shown with black squares indicating the insets of the areas shown at higher magnification. (d) Immunofluorescent staining of endomucin (Endo) and Smooth Muscle Actin (SMA) in OV-90 +/+ and OV-90 −/− xenografts, with nuclei stained with DAPI, scale bar 200 µm. White squares indicate the insets of the areas shown at higher magnification. Representative images are shown, together with quantification of total (Endo + ) and pericyte negative (Endo + SMA − ) vessels. (e) Ultrasound images of OV-90 +/+ and OV-90 −/− xenografts. Incoming (red) and outcoming (blue) blood flows are shown. The graph indicates the percentage of the blood flow areas detected within each tumor (n=3 per group). One-tailed t-test was used to compare averages between the groups. (f) Immunofluorescent staining of F4/80 + macrophages (red) in OV-90 +/+ and OV-90 −/− xenografts. White squares indicate the insets of the areas shown at higher magnification. The arrows indicate F4/80 + cells. Nuclei are stained with DAPI (blue), scale bar = 20 µm. For all panels, data in graphs are represented as mean ± SEM and p-value is indicated.
Article Snippet: The human HGSOC cell line OV90 was purchased from ATCC® (Manassas, VA, USA #CRL-3585).
Techniques: Western Blot, Control, Gene Expression, Quantitative RT-PCR, Transformation Assay, Staining, One-tailed Test
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