rabbit polyclonal antibody to the c-terminus of the long-form ovine leptin receptor orb6312  (Biorbyt)

Journal: Endocrinology

Article Title: Leptin Matures Aspects of Lung Structure and Function in the Ovine Fetus

doi: 10.1210/en.2015-1729

Figure Lengend Snippet: Antibody Table

Article Snippet: Ovine long-form leptin receptor , KLH-conjugated synthetic peptide derived from sheep leptin receptor C terminus , Antileptin receptor , Biorbyt, orb6312 , Rabbit; polyclonal , 2 μg/mL.

Techniques: Sequencing, Derivative Assay

Journal: Endocrinology

Article Title: Leptin Matures Aspects of Lung Structure and Function in the Ovine Fetus

doi: 10.1210/en.2015-1729

Figure Lengend Snippet: Antibody Table

Article Snippet: Ovine long-form leptin receptor , KLH-conjugated synthetic peptide derived from sheep leptin receptor C terminus , Antileptin receptor , Biorbyt, orb6312 , Rabbit; polyclonal , 2 μg/mL.

Techniques: Sequencing, Derivative Assay

Journal: Endocrinology

Article Title: Leptin Matures Aspects of Lung Structure and Function in the Ovine Fetus

doi: 10.1210/en.2015-1729

Figure Lengend Snippet: Antibody Table

Article Snippet: Equal amounts of sample protein were separated using 7.5% Mini-PROTEAN precast gels followed by incubation overnight at 4°C with rabbit polyclonal primary antibodies ( ) to 1) the ovine long-form leptin receptor (Ob-Rb) (2-μg/mL orb6312; Biorbyt); 2) the mouse phosphorylated STAT3 containing Ser727 (0.5-μg/mL sc-8001-R; Insight Biotechnology Ltd); or 3) the human STAT3 (1:500, 07-2173; Millipore) ( ).

Techniques: Sequencing, Derivative Assay

Journal: Endocrinology

Article Title: Leptin Matures Aspects of Lung Structure and Function in the Ovine Fetus

doi: 10.1210/en.2015-1729

Figure Lengend Snippet: Mean ± SEM relative mRNA abundance of (A) all forms of the leptin receptor Ob-R and (B) the long-form leptin receptor Ob-Rb, and relative protein expression of (C) the long-form leptin receptor and (D) phosphorylated STAT3 relative to total STAT3, in the lungs of sheep fetuses infused iv with either saline or leptin (0.5 or 1.0 LEP) for 5 days. †, significantly different from saline group, unpaired Student's t test, P < .05.

Article Snippet: Equal amounts of sample protein were separated using 7.5% Mini-PROTEAN precast gels followed by incubation overnight at 4°C with rabbit polyclonal primary antibodies ( ) to 1) the ovine long-form leptin receptor (Ob-Rb) (2-μg/mL orb6312; Biorbyt); 2) the mouse phosphorylated STAT3 containing Ser727 (0.5-μg/mL sc-8001-R; Insight Biotechnology Ltd); or 3) the human STAT3 (1:500, 07-2173; Millipore) ( ).

Techniques: Expressing, Saline