Journal: Frontiers in Cardiovascular Medicine
Article Title: PACAP regulates VPAC1 expression, inflammatory processes and lipid homeostasis in M1- and M2-macrophages
doi: 10.3389/fcvm.2023.1264901
Figure Lengend Snippet: Effects of PACAP deficiency on the distribution and amount of different MΦ subtypes of atherosclerotic plaques in the aortic arch. Immunohistomorphometric analyses of atherosclerotic lesions in ApoE −/− and PACAP −/− /ApoE −/− mice after SC or CED. Analyses of VPAC1 ( A ), CD86 ( B ), CD68 ( C ), and CD163 ( D ) on cryosections of the aortic arch and representative photomicrographs ( E–T ) are shown. The graph bar is given as the mean. Black dots represent single measurement. ( n = 3–8). * p ≤ 0.05 vs. ApoE −/− after 30 weeks of SC; # p ≤ 0.05; ### p ≤ 0.001 vs. ApoE −/− after 20 weeks of CED. SC, standard diet; CED, cholesterol-enriched diet; n, number of experimental animals; Black arrows, positive reaction; asterisks, vessel lumen. Scale bar = 10 μm; Overview screen scale bar = 100 µm.
Article Snippet: Rabbit anti-mouse CD163 , Orb182468 , Biorbyt, Cambridge, UK , 1:100.
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