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operetta cls high content analysis system  (Revvity)


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    Structured Review

    Revvity operetta cls high content analysis system
    Operetta Cls High Content Analysis System, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 3404 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/operetta/Operetta+CLS+high-content+analysis+system/10__1016_slash_j__scr__2026__104112-71-5-11
    Average 96 stars, based on 3404 article reviews
    operetta cls high content analysis system - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Fluorescence In Situ Hybridization:

    Article Title: c-Myc mRNA translation modulators and uses thereof in the treatment of cancer
    Article Snippet: Following RNA FISH experiments, images of cells were taken with Operetta (Perkin Elmer, USA), a wide-field fluorescence microscope at ×63 magnification. .. Following RNA FISH experiments, images of cells were taken with Operetta (Perkin Elmer, USA), a wide-field fluorescence microscope at ×63 magnification. .. After acquisition, the images were transferred to Columbus software for image analysis.

    Article Title: c-MYC mRNA translation modulators and uses thereof in the treatment of cancer
    Article Snippet: Following RNA FISH experiments, images of cells were taken with Operetta (Perkin Elmer, USA), a wide-field fluorescence microscope at ×63 magnification. .. Following RNA FISH experiments, images of cells were taken with Operetta (Perkin Elmer, USA), a wide-field fluorescence microscope at ×63 magnification. ..

    Fluorescence:

    Article Title: c-Myc mRNA translation modulators and uses thereof in the treatment of cancer
    Article Snippet: Following RNA FISH experiments, images of cells were taken with Operetta (Perkin Elmer, USA), a wide-field fluorescence microscope at ×63 magnification. .. Following RNA FISH experiments, images of cells were taken with Operetta (Perkin Elmer, USA), a wide-field fluorescence microscope at ×63 magnification. .. After acquisition, the images were transferred to Columbus software for image analysis.

    Article Title: c-MYC mRNA translation modulators and uses thereof in the treatment of cancer
    Article Snippet: Following RNA FISH experiments, images of cells were taken with Operetta (Perkin Elmer, USA), a wide-field fluorescence microscope at ×63 magnification. .. Following RNA FISH experiments, images of cells were taken with Operetta (Perkin Elmer, USA), a wide-field fluorescence microscope at ×63 magnification. ..

    Article Title: Epigenetic Drugs Splitomicin, Suberohydroxamic Acid, CPTH6, BVT-948, and PBIT Moderate Fibro-Fatty Development in Arrhythmogenic Cardiomyopathy
    Article Snippet: .. The pellet was resuspended in MM and cells were seeded into 96-multiwell plates (PerkinElmer, Waltham, MA, USA) optimized for fluorescence detection by Operetta ® (PerkinElmer) at a final density of 4.7 × 10 cells/cm 2 . .. After 24 h of cell attachment, CMSCs were cultured for 6 days in either adipogenic [ ] or pro-fibrotic [ ] media, as previously described, with media refreshed every 72 h. For adipogenic stimulation, cells were cultured in IMDM supplemented with 10% of FBS, 0.1 of mM indomethacin (Sigma Aldrich, St. Louis, MO, USA), 1 μM of hydrocortisone (Sigma Aldrich), and 0.5 mM of 3-isobutyl-methylxanthine (Sigma Aldrich).

    Article Title: Loss of PREPL alters lipid homeostasis due to mitochondrial defects
    Article Snippet: .. Fluorescence was measured using a high-content screening microscope, Operetta (CLS+Twister; PerkinElmer), and lipid droplet size and number were quantified using Harmony 4.9 software (PerkinElmer). .. Immunofluorescence – Staining of individual peroxisomes, as well as co-staining of PREPL and peroxisomes, was performed using the PEX14 ( ) and PREPL abnova Maxpab (H00009581-B01P) ( ) antibodies, with Alexa-conjugated secondary fluorophores.

    Article Title: Epigenetic Drugs Splitomicin, Suberohydroxamic Acid, CPTH6, BVT-948, and PBIT Moderate Fibro-Fatty Development in Arrhythmogenic Cardiomyopathy
    Article Snippet: .. The pellet was resuspended in MM and cells were seeded into 96-multiwell plates (PerkinElmer, Waltham, MA, USA) optimized for fluorescence detection by Operetta® (PerkinElmer) at a final density of 4.7× 104 cells/cm2. .. After 24 h of cell attachment, CMSCs were cultured for 6 days in either adipogenic [3] or pro-fibrotic [4] media, as previously described, with media refreshed every 72 h. For adipogenic stimulation, cells were cultured in IMDM supplemented with 10% of FBS, 0.1 of mM indomethacin (Sigma Aldrich, St. Louis, MO, USA), 1 μM of hydrocortisone (Sigma Aldrich), and 0.5 mM of 3-isobutyl-methylxanthine (Sigma Aldrich).

    Microscopy:

    Article Title: c-Myc mRNA translation modulators and uses thereof in the treatment of cancer
    Article Snippet: Following RNA FISH experiments, images of cells were taken with Operetta (Perkin Elmer, USA), a wide-field fluorescence microscope at ×63 magnification. .. Following RNA FISH experiments, images of cells were taken with Operetta (Perkin Elmer, USA), a wide-field fluorescence microscope at ×63 magnification. .. After acquisition, the images were transferred to Columbus software for image analysis.

    Article Title: c-MYC mRNA translation modulators and uses thereof in the treatment of cancer
    Article Snippet: Following RNA FISH experiments, images of cells were taken with Operetta (Perkin Elmer, USA), a wide-field fluorescence microscope at ×63 magnification. .. Following RNA FISH experiments, images of cells were taken with Operetta (Perkin Elmer, USA), a wide-field fluorescence microscope at ×63 magnification. ..

    Article Title: Loss of PREPL alters lipid homeostasis due to mitochondrial defects
    Article Snippet: .. Fluorescence was measured using a high-content screening microscope, Operetta (CLS+Twister; PerkinElmer), and lipid droplet size and number were quantified using Harmony 4.9 software (PerkinElmer). .. Immunofluorescence – Staining of individual peroxisomes, as well as co-staining of PREPL and peroxisomes, was performed using the PEX14 ( ) and PREPL abnova Maxpab (H00009581-B01P) ( ) antibodies, with Alexa-conjugated secondary fluorophores.

    Incubation:

    Article Title: Feeder Cell Detachment in Drug Response Profiling of Leukemia Cell Coculture Can Be Prevented by Conditioned Medium
    Article Snippet: .. After 72 h of incubation with chemical compounds, living cells were stained by CyQUANT Direct Cell Proliferation Assay (Thermo Fisher Scientific, MA, USA) and automatically imaged (Operetta, PerkinElmer/Revvity, MA, USA). .. Acquired images were analyzed using Columbus software (PerkinElmer/Revvity, MA, USA).

    Staining:

    Article Title: Feeder Cell Detachment in Drug Response Profiling of Leukemia Cell Coculture Can Be Prevented by Conditioned Medium
    Article Snippet: .. After 72 h of incubation with chemical compounds, living cells were stained by CyQUANT Direct Cell Proliferation Assay (Thermo Fisher Scientific, MA, USA) and automatically imaged (Operetta, PerkinElmer/Revvity, MA, USA). .. Acquired images were analyzed using Columbus software (PerkinElmer/Revvity, MA, USA).

    CyQUANT Assay:

    Article Title: Feeder Cell Detachment in Drug Response Profiling of Leukemia Cell Coculture Can Be Prevented by Conditioned Medium
    Article Snippet: .. After 72 h of incubation with chemical compounds, living cells were stained by CyQUANT Direct Cell Proliferation Assay (Thermo Fisher Scientific, MA, USA) and automatically imaged (Operetta, PerkinElmer/Revvity, MA, USA). .. Acquired images were analyzed using Columbus software (PerkinElmer/Revvity, MA, USA).

    Proliferation Assay:

    Article Title: Feeder Cell Detachment in Drug Response Profiling of Leukemia Cell Coculture Can Be Prevented by Conditioned Medium
    Article Snippet: .. After 72 h of incubation with chemical compounds, living cells were stained by CyQUANT Direct Cell Proliferation Assay (Thermo Fisher Scientific, MA, USA) and automatically imaged (Operetta, PerkinElmer/Revvity, MA, USA). .. Acquired images were analyzed using Columbus software (PerkinElmer/Revvity, MA, USA).

    High Content Screening:

    Article Title: Loss of PREPL alters lipid homeostasis due to mitochondrial defects
    Article Snippet: .. Fluorescence was measured using a high-content screening microscope, Operetta (CLS+Twister; PerkinElmer), and lipid droplet size and number were quantified using Harmony 4.9 software (PerkinElmer). .. Immunofluorescence – Staining of individual peroxisomes, as well as co-staining of PREPL and peroxisomes, was performed using the PEX14 ( ) and PREPL abnova Maxpab (H00009581-B01P) ( ) antibodies, with Alexa-conjugated secondary fluorophores.

    Article Title: Lipid peroxidation and type I interferon coupling fuels pathogenic macrophage activation causing tuberculosis susceptibility
    Article Snippet: Software, algorithm , Enrichr , Enrichr , RRID: SCR_001575 , . .. Other , Operetta CLS HCA System , Operetta , https://www.perkinelmer.com/in/lab-solutions/product/operetta-cls-system-hh16000020 , . .. Other , Vibratome , Leica VT1200 S , https://www.leicabiosystems.com/us/research/vibratomes/leica-vt1200/ , .

    Software:

    Article Title: Loss of PREPL alters lipid homeostasis due to mitochondrial defects
    Article Snippet: .. Fluorescence was measured using a high-content screening microscope, Operetta (CLS+Twister; PerkinElmer), and lipid droplet size and number were quantified using Harmony 4.9 software (PerkinElmer). .. Immunofluorescence – Staining of individual peroxisomes, as well as co-staining of PREPL and peroxisomes, was performed using the PEX14 ( ) and PREPL abnova Maxpab (H00009581-B01P) ( ) antibodies, with Alexa-conjugated secondary fluorophores.



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    Revvity live cell confocal imaging
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    Image Search Results


    (A) Detection of SARS-CoV-2 infected cells using high-content imaging under control conditions. Fluorescent signals: green (anti-SARS-CoV NP mAb), blue (Hoechst). (B) Representative flow cytometry analysis plots showing NP+ cells. (C) Measurement of fluorescent intensity in NP+ cells. (D) Percentage of infected MSCs. (E) The supernatants of infected MSCs were collected and titrated on Vero-E6/TMPRSS2 cells to measure infectious viral particle production using the focus-forming assay. (F) After RNA extraction from infected MSCs, the expression of IDO1 was examined using real-time PCR. The graph displays the expression of IDO1 normalized to GAPDH , with the mock group serving as the control. Data are represented as means ± SEM from three independent biological replicates. For , the Delta condition was available from only two independent donors and was therefore presented descriptively and excluded from the statistical analysis. Statistical comparisons among the remaining groups were performed using one-way ANOVA followed by Tukey’s multiple-comparisons test. One-way ANOVA showed a significant overall difference among groups, F(2, 6) = 6.794, P = 0.0287. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.

    Journal: PLOS One

    Article Title: Omicron SARS-CoV-2 shows NRP1-linked susceptibility in adipose-derived MSCs and alters primitive cord blood CD34⁺ HSPCs

    doi: 10.1371/journal.pone.0360439

    Figure Lengend Snippet: (A) Detection of SARS-CoV-2 infected cells using high-content imaging under control conditions. Fluorescent signals: green (anti-SARS-CoV NP mAb), blue (Hoechst). (B) Representative flow cytometry analysis plots showing NP+ cells. (C) Measurement of fluorescent intensity in NP+ cells. (D) Percentage of infected MSCs. (E) The supernatants of infected MSCs were collected and titrated on Vero-E6/TMPRSS2 cells to measure infectious viral particle production using the focus-forming assay. (F) After RNA extraction from infected MSCs, the expression of IDO1 was examined using real-time PCR. The graph displays the expression of IDO1 normalized to GAPDH , with the mock group serving as the control. Data are represented as means ± SEM from three independent biological replicates. For , the Delta condition was available from only two independent donors and was therefore presented descriptively and excluded from the statistical analysis. Statistical comparisons among the remaining groups were performed using one-way ANOVA followed by Tukey’s multiple-comparisons test. One-way ANOVA showed a significant overall difference among groups, F(2, 6) = 6.794, P = 0.0287. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.

    Article Snippet: The Operetta high-content imaging system (PerkinElmer) was used to detect and analyze the fluorescent signals.

    Techniques: Infection, Imaging, Control, Flow Cytometry, Analysis, Focus Forming Assay, RNA Extraction, Expressing, Real-time Polymerase Chain Reaction

    (A) Representative western blot depicting the expression of NRP1 in AD-MSCs (No siRNA), cells transfected with non-targeting siRNA (siControl), or siNRP1 for 24 hours. β-actin was used as a loading control. (B) Graph displaying the quantification of NRP1 normalized to β-actin (n = 3). This experiment represents three independent biological replications. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. (C) Transfected cells were visualized using high-content imaging. Fluorescent signals: green for anti-NRP1 mAb, blue for Hoechst. (D) Percentage of NRP1-expressing cells normalized to cells transfected with siControl. Data are presented as mean ± SEM from three independent experiments. Statistical analysis was conducted using a paired t-test. Next, siRNA-transfected cells were infected with different variants of SARS-CoV-2. (E) SARS-CoV-2-infected cells were detected using high-content imaging. Fluorescent signals: green for anti-SARS-CoV NP mAb, blue for Hoechst. (F) The fluorescent intensity of NP+ cells was measured. All data represent three independent experiments. Statistical analysis among each group of variant was performed using an unpaired t-test. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.

    Journal: PLOS One

    Article Title: Omicron SARS-CoV-2 shows NRP1-linked susceptibility in adipose-derived MSCs and alters primitive cord blood CD34⁺ HSPCs

    doi: 10.1371/journal.pone.0360439

    Figure Lengend Snippet: (A) Representative western blot depicting the expression of NRP1 in AD-MSCs (No siRNA), cells transfected with non-targeting siRNA (siControl), or siNRP1 for 24 hours. β-actin was used as a loading control. (B) Graph displaying the quantification of NRP1 normalized to β-actin (n = 3). This experiment represents three independent biological replications. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. (C) Transfected cells were visualized using high-content imaging. Fluorescent signals: green for anti-NRP1 mAb, blue for Hoechst. (D) Percentage of NRP1-expressing cells normalized to cells transfected with siControl. Data are presented as mean ± SEM from three independent experiments. Statistical analysis was conducted using a paired t-test. Next, siRNA-transfected cells were infected with different variants of SARS-CoV-2. (E) SARS-CoV-2-infected cells were detected using high-content imaging. Fluorescent signals: green for anti-SARS-CoV NP mAb, blue for Hoechst. (F) The fluorescent intensity of NP+ cells was measured. All data represent three independent experiments. Statistical analysis among each group of variant was performed using an unpaired t-test. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.

    Article Snippet: The Operetta high-content imaging system (PerkinElmer) was used to detect and analyze the fluorescent signals.

    Techniques: Western Blot, Expressing, Transfection, Control, Analysis, Imaging, Infection, Variant Assay