primer oligo(dt) 15 (Promega)
90
Structured Review
Promega
primer oligo(dt) 15
Primer Oligo(dt) 15, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/oligo(dt)15/oligo+dt++primers/pmc12259917-118-7-12
Average 90 stars, based on 1 article reviews
Primer Oligo(dt) 15, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/oligo(dt)15/oligo+dt++primers/pmc12259917-118-7-12
Average 90 stars, based on 1 article reviews
primer oligo(dt) 15 - by Bioz Stars,
2026-10
90/100 stars
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Isolation:Article Title: Ischemia induced peroxynitrite dependent modifications of cardiomyocyte MLC1 increases its degradation by MMP-2 leading to contractile dysfunction Article Snippet: .. The cDNA clone for the human ventricular MLC1 (NCBI # NM_000258) was isolated with a two-tube RT-PCR method and Omniscript RT kit (Qiagen, Germantown, MD, USA) using total adult human heart RNA (Stratagene, La Jolla, CA, USA), Article Title: Alteration in the processing of the ACRBP/sp32 protein and sperm head/acrosome malformations in proprotein convertase 4 (PCSK4) null mice Article Snippet: .. Briefly, total RNA (2 μg) was isolated using TRIzol procedure (Invitrogen, Grand Island, NY) from mouse testes flash-frozen in liquid nitrogen and reverse-transcribed according to the manufacturer's instructions with 200 units of superscript III reverse transcriptase (Invitrogen) and 25 ng/ml Article Title: Neonatal asphyxia induces the nitration of cardiac myosin light chain 2 (MLC2) which is associated with cardiac systolic dysfunction Article Snippet: .. Preparation of recombinant human cardiac MLC2 The cDNA clone for the human ventricular myosin light chain 2 (MLC2) (NCBI # AAB07462 ) was isolated with a two-tube RT-PCR method and Omniscript RT kit (Qiagen) using total adult human heart RNA (Stratagene), Article Title: Neonatal asphyxia induces the nitration of cardiac myosin light chain 2 (MLC2) which is associated with cardiac systolic dysfunction Article Snippet: .. The cDNA clone for the human ventricular myosin light chain 2 (MLC2) (NCBI # AAB07462 ) was isolated with a two-tube RT-PCR method and Omniscript RT kit (Qiagen) using total adult human heart RNA (Stratagene), Reverse Transcription Polymerase Chain Reaction:Article Title: Ischemia induced peroxynitrite dependent modifications of cardiomyocyte MLC1 increases its degradation by MMP-2 leading to contractile dysfunction Article Snippet: .. The cDNA clone for the human ventricular MLC1 (NCBI # NM_000258) was isolated with a two-tube RT-PCR method and Omniscript RT kit (Qiagen, Germantown, MD, USA) using total adult human heart RNA (Stratagene, La Jolla, CA, USA), Article Title: Neonatal asphyxia induces the nitration of cardiac myosin light chain 2 (MLC2) which is associated with cardiac systolic dysfunction Article Snippet: .. Preparation of recombinant human cardiac MLC2 The cDNA clone for the human ventricular myosin light chain 2 (MLC2) (NCBI # AAB07462 ) was isolated with a two-tube RT-PCR method and Omniscript RT kit (Qiagen) using total adult human heart RNA (Stratagene), Article Title: Neonatal asphyxia induces the nitration of cardiac myosin light chain 2 (MLC2) which is associated with cardiac systolic dysfunction Article Snippet: .. The cDNA clone for the human ventricular myosin light chain 2 (MLC2) (NCBI # AAB07462 ) was isolated with a two-tube RT-PCR method and Omniscript RT kit (Qiagen) using total adult human heart RNA (Stratagene), Reverse Transcription:Article Title: Alteration in the processing of the ACRBP/sp32 protein and sperm head/acrosome malformations in proprotein convertase 4 (PCSK4) null mice Article Snippet: .. Briefly, total RNA (2 μg) was isolated using TRIzol procedure (Invitrogen, Grand Island, NY) from mouse testes flash-frozen in liquid nitrogen and reverse-transcribed according to the manufacturer's instructions with 200 units of superscript III reverse transcriptase (Invitrogen) and 25 ng/ml Article Title: Wound Healing Improvement with PHD-2 Silenced Fibroblasts in Diabetic Mice Article Snippet: Total RNA from skin samples was isolated by using Trizol reagent (Invitrogen, Life technology, USA), according to the manufacturer’s protocol. .. 1μg RNA sample was reverse-transcribed with Article Title: Autocrine VEGF signalling on M2 macrophages regulates PD ‐L1 expression for immunomodulation of T cells Article Snippet: The RNA was resuspended in RNase‐free water and the RNA concentration was determined by light absorbance at 260 nm (MaestroNano, Maestrogen, Taiwan). .. Total RNA (1 μg) was used in the reverse transcription (RT) reaction with 0.5 μg of Article Title: Altered Peroxisome-Proliferator Activated Receptors Expression in Human Endometrial Cancer Article Snippet: .. RNA integrity was verified by elecrophoresis in 1.5% agarose gel and staining with ethidium bromide, and by amplification of housekeeping gene, 18s rRNA, 1 μ g of total RNA was used to prepare cDNA. cDNA synthesis was performed in 50 mM Tris-HCL (pH 8.3), 75 mM KCl, 3 mM MgCl 2 , 10 mM DTT 1 mM dNTP mix (Promega), 2.5 μ Recombinant:Article Title: Neonatal asphyxia induces the nitration of cardiac myosin light chain 2 (MLC2) which is associated with cardiac systolic dysfunction Article Snippet: .. Preparation of recombinant human cardiac MLC2 The cDNA clone for the human ventricular myosin light chain 2 (MLC2) (NCBI # AAB07462 ) was isolated with a two-tube RT-PCR method and Omniscript RT kit (Qiagen) using total adult human heart RNA (Stratagene), Agarose Gel Electrophoresis:Article Title: Altered Peroxisome-Proliferator Activated Receptors Expression in Human Endometrial Cancer Article Snippet: .. RNA integrity was verified by elecrophoresis in 1.5% agarose gel and staining with ethidium bromide, and by amplification of housekeeping gene, 18s rRNA, 1 μ g of total RNA was used to prepare cDNA. cDNA synthesis was performed in 50 mM Tris-HCL (pH 8.3), 75 mM KCl, 3 mM MgCl 2 , 10 mM DTT 1 mM dNTP mix (Promega), 2.5 μ Staining:Article Title: Altered Peroxisome-Proliferator Activated Receptors Expression in Human Endometrial Cancer Article Snippet: .. RNA integrity was verified by elecrophoresis in 1.5% agarose gel and staining with ethidium bromide, and by amplification of housekeeping gene, 18s rRNA, 1 μ g of total RNA was used to prepare cDNA. cDNA synthesis was performed in 50 mM Tris-HCL (pH 8.3), 75 mM KCl, 3 mM MgCl 2 , 10 mM DTT 1 mM dNTP mix (Promega), 2.5 μ Amplification:Article Title: Altered Peroxisome-Proliferator Activated Receptors Expression in Human Endometrial Cancer Article Snippet: .. RNA integrity was verified by elecrophoresis in 1.5% agarose gel and staining with ethidium bromide, and by amplification of housekeeping gene, 18s rRNA, 1 μ g of total RNA was used to prepare cDNA. cDNA synthesis was performed in 50 mM Tris-HCL (pH 8.3), 75 mM KCl, 3 mM MgCl 2 , 10 mM DTT 1 mM dNTP mix (Promega), 2.5 μ cDNA Synthesis:Article Title: Altered Peroxisome-Proliferator Activated Receptors Expression in Human Endometrial Cancer Article Snippet: .. RNA integrity was verified by elecrophoresis in 1.5% agarose gel and staining with ethidium bromide, and by amplification of housekeeping gene, 18s rRNA, 1 μ g of total RNA was used to prepare cDNA. cDNA synthesis was performed in 50 mM Tris-HCL (pH 8.3), 75 mM KCl, 3 mM MgCl 2 , 10 mM DTT 1 mM dNTP mix (Promega), 2.5 μ |