We use essential cookies to operate our site. With your consent, we may also use non-essential cookies to improve your user experience and to analyze website traffic. Click "Accept" to accept cookies and go directly to the site, click "Reject" to reject all but cookies strictly necessary for the functioning of this site. You can reset your cookie settings at any time by visiting your Bioz "my account" page and selecting the "reset cookie preferences" link.

Accept Reject

Overview
Article Snippets
home > search results > product details
star_border
     Loading Product Details ...      Welcome to Your Next Discovery   
86 / 100
Bioz Stars
OH 44118
Affinity Therapeutics  
Product Name
Saccharomyces cerevisiae Meyen ex E C Hansen
Catalog Number
4023352
Biosafety Level
1
Genotype
MATa/MATalpha his3delta1/his3delta1 leu2delta0/leu2delta0 lys2delta0/+ met15delta0/+ ura3delta0/ura3delta0 deltaNGR1
Category
Fungi
Product Format
Frozen
More Catalog Fields ...
All
Imaging
Knockdown
Microscopy
Negative Control
Software
Super-Resolution Microscopy
Transfection
Article Snippets for All Techniques
Article Snippets for All Techniques
Mitochondrial Alkbh1 localizes to mtRNA granules and its knockdown induces the mitochondrial UPR in humans and C. elegans .
"Super resolution imaging was performed with a 3D SIM Deltavision OMX V3 microscope (General Electric) equipped with a 100 × 1.4 oil immersion objective UPlanSApo (Olympus), 405 nm, 488 nm and 593 nm diode lasers and Cascade II EMCCD cameras (Photometrics). .. Knockdown of human Alkbh1 with siRNAs 293 cells were transfected with siRNAs (Life Technologies) targeting human Alkbh1 using 50nM siRNA and Lipofectamine 2000 (Invitrogen) in Opti-MEM according to manufacturer´s instructions."
Mitochondrial Alkbh1 localizes to mtRNA granules and its knockdown induces the mitochondrial UPR in humans and C. elegans .
"Knockdown of human Alkbh1 with siRNAs 293 cells were transfected with siRNAs (Life Technologies) targeting human Alkbh1 using 50nM siRNA and Lipofectamine 2000 (Invitrogen) in Opti-MEM according to manufacturer´s instructions."
Mitochondrial Alkbh1 localizes to mtRNA granules and its knockdown induces the mitochondrial UPR in humans and C. elegans .
"Knockdown of human Alkbh1 with siRNAs 293 cells were transfected with siRNAs (Life Technologies) targeting human Alkbh1 using 50nM siRNA and Lipofectamine 2000 (Invitrogen) in Opti-MEM according to manufacturer´s instructions. .. As negative control we used the SilencerTM Negative control No.1 siRNA (AM4611, Life Technologies)."
Mitochondrial Alkbh1 localizes to mtRNA granules and its knockdown induces the mitochondrial UPR in humans and C. elegans .
"In a second step, a custom-made macro in Fiji (Schindelin et al., 2012) was used to finalize the channel alignment and to establish composite TIFF stacks that are subsequently loaded as leveled RGB images into the Volocity calculation software (Volocity 6.1.2 (Perkin Elmer)). .. Knockdown of human Alkbh1 with siRNAs 293 cells were transfected with siRNAs (Life Technologies) targeting human Alkbh1 using 50nM siRNA and Lipofectamine 2000 (Invitrogen) in Opti-MEM according to manufacturer´s instructions."