nup205 (Proteintech)
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nup205
Nup205, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nup205/NUP205+Antibody/pmc13005589-43-13-15
Average 93 stars, based on 13 article reviews
Nup205, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nup205/NUP205+Antibody/pmc13005589-43-13-15
Average 93 stars, based on 13 article reviews
nup205 - by Bioz Stars,
2026-09
93/100 stars
Images
1) Product Images from "NUP205 Stabilized YAP1 Protein to Stimulate Growth of Hepatocellular Carcinoma Cells in vitro and in vivo"
Article Title: NUP205 Stabilized YAP1 Protein to Stimulate Growth of Hepatocellular Carcinoma Cells in vitro and in vivo
Journal: Journal of Hepatocellular Carcinoma
doi: 10.2147/JHC.S560643
Figure Legend Snippet: NUP205 expression in cancer and non-tumor adjacent tissues from TCGA database. ( A ) NUP205 mRNA expression in tumor (red bars) and non-tumor (blue bars) samples; ( B ) NUP205 mRNA expression in HCC (n = 371) and normal tissues (n = 50) from TCGA database; ( C ) NUP205 mRNA expression in HCC samples from TCGA by grade; ( D ) IHC detection of NUP205 expression in 16 paired patient samples of HCC and adjacent normal tissues. Representative images are shown to the left with quantitative analysis to the right. Scale bars: 100 μm. Bars represent mean ± SD. ****p < 0.0001; ns = not significant (Log rank test).
Techniques Used: Expressing
Figure Legend Snippet: NUP205 expression and HCC prognosis. ( A ) Kaplan-Meier OS curves for HCC patients from TCGA database with high or low NUP205 expression; ( B ) Kaplan-Meier DSS curves for HCC patients from TCGA database with high or low NUP205 expression; ( C ) Effect of NUP205 expression on survival of HCC patients from TCGA database; ( D and E ) Kaplan-Meier OS curves for male and female HCC patients from TCGA database with low or high NUP205. p values calculated by Log rank test.
Techniques Used: Expressing
Figure Legend Snippet: NUP205 expression in HCC cell lines and effect of NUP205 silencing on proliferation. ( A and B ) NUP205 mRNA and protein expression in HCC cells; ( C and D ) Silencing of NUP205 by transfection with siNUP205 siRNAs. Effects on NUP205 protein ( C ) and mRNA ( D ) in HCC LM3 and SK-Hep1 cells are shown; ( E and F ) Viability of HCC LM3 and SK-Hep1 cells transfected with siNUP205 measured by CCK-8 assay; ( G and H ) Colony formation by HCC LM3 and SK-Hep1 cells transfected with siNUP205. Representative images are shown on the left with quantitative analysis on the right; ( I and J ) Proliferation of HCC LM3 and SK-Hep1 cells transfected with siNUP205 measured by EDU assay. Representative images are shown on the left with quantitative analysis on the right. Bars represent mean ± SD. # Indicates a separator and has no practical meaning. *p < 0.05, **p < 0.01, ****p < 0.0001 (one-way ANOVA multiple comparison). Scale bars: 100 μm.
Techniques Used: Expressing, Transfection, CCK-8 Assay, EdU Assay, Comparison
Figure Legend Snippet: Effects of NUP205 overexpression on HCC cell proliferation. ( A and B ) Expression of NUP205 mRNA assessed by RT-qPCR and of NUP205 protein assessed by Western blotting following transfection of HCC LM3 and SK-Hep1 cells with NUP205 overexpressing plasmid; ( C and D ) Growth curves constructed from CCK-8 assays of HCC LM3 and SK-Hep1 cell viability following transfection with NUP205 overexpressing plasmid; ( E ) Colony formation by HCC LM3 and SK-Hep1 cells following transfection with NUP205 overexpressing plasmid. Bars represent mean ± SD. # Indicates a separator and has no practical meaning. *p < 0.05, **p < 0.01, ****p < 0.0001, ns = not significant by two-tailed Student’s t -test and one-way ANOVA multiple comparison.
Techniques Used: Over Expression, Expressing, Quantitative RT-PCR, Western Blot, Transfection, Plasmid Preparation, Construct, CCK-8 Assay, Two Tailed Test, Comparison
Figure Legend Snippet: Effects of NUP205 silencing on apoptosis in HCC LM3 and SK-Hep1 cells. ( A ) Measurements of MMP by JC-1 assay in HCC LM3 and SK-Hep1 cells after transfection with siNUP205. Representative images are shown on the left with quantitative analysis of green/red fluorescence on the right; ( B ) Measurements of apoptosis by Annexin V/PI assay in HCC LM3 and SK-Hep1 cells after transfection with siNUP205. Representative flow cytometry images are shown on the left with quantitative analysis of apoptotic cells on the right; ( C ) Hoechst staining to show nuclear changes in HCC LM3 and SK-Hep1 cells after transfection with siNUP205. Representative images are shown with white arrows indicating apoptotic nuclei. Scale bar: 100 μm; ( D ) Expression of effectors involved in apoptosis in HCC LM3 and SK-Hep1 cells after transfection with siNUP205. Protein expression is shown in the gel (left) and mRNA expression in the plots (right). Expression was normalized to β-actin. Bars represent mean ± SD. # Indicates a separator and has no practical meaning. *p < 0.05, ***p < 0.001, ****p < 0.0001 by one-way ANOVA with multiple comparison.
Techniques Used: Transfection, Fluorescence, Flow Cytometry, Staining, Expressing, Comparison
Figure Legend Snippet: Effects of NUP205 silencing on growth of human HCC xenograft tumors in nude mice. ( A and B ) Photographic images of mice and excised tumors from control (siNC injected) and NUP205 knockdown (siNUP205 transfected) animals; ( C ) Tumor mass at 33 days from control and NUP205 silenced tumors; ( D ) Time course of tumor volume in siNC and siNUP205 treated mice; ( E ) Time course of relative tumor volume in siNC and siNUP205 treated mice. Relative tumor volume was calculated as (tumor volume siNUP205)/ (tumor volume siNC) × 100; ( F ) Time course of mouse body weight during treatment; ( G ) Ki67 expression by siNC and siNUP205 treated tumor tissues detected by IHC. Representative images are shown on the left with quantitative analysis on the right; ( H ) Apoptotic cells in tumors from siNC and siNUP205 treated animals assessed by TUNEL staining. Representative images are shown on the left with quantitative analysis on the right; ( I ) Tumor tissue morphology assessed by H&E staining; ( J ) Representative Western blotting images showing expression of Cleaved PARP, Caspase 9, Cleaved caspase 9, Bcl-2, BAX, Caspase7 and Cleaved caspase 3/7 proteins in tumors treated with siNC or siNUP205 (n = 6). Image scale bars: 50 μm, 20 μm. Bar charts represent mean ± SD. # Indicates a separator and has no practical meaning. *p < 0.05, **p < 0.01, ***p < 0.001 by two- tailed Student’s t -test.
Techniques Used: Control, Injection, Knockdown, Transfection, Expressing, TUNEL Assay, Staining, Western Blot, Two Tailed Test
Figure Legend Snippet: Effects of NUP205 on YAP1 expression. ( A and B ) YAP1 mRNA expression assessed by RT-qPCR in HCC LM3 and SK-Hep1cells with NUP205 knockdown ( A ) or overexpression ( B ); ( C ) YAP1 protein expression assessed by Western blotting in HCC LM3 and SK-Hep1 cells with NUP205 overexpression. Representative image shown in upper panel with quantitative analysis in lower panel; ( D ) YAP1 protein expression assessed by Western blotting in HCC LM3 and SK-Hep1 cells with NUP205 silencing. Representative image on the left with quantitative analysis on the right; ( E and F ) Expression of YAP1 mRNA and protein in human xenograft HCC tumors in a mouse model treated with siNC (control) or siNUP205 (NUP205 silencing); ( G and H ) Representative immunofluorescence staining images to show the localization of YAP1 in HCC LM3 and SK-Hep1 transfected with siNC (control) or siNUP205 (NUP205 knockdown, n = 3). Scale bars: 20 μm. Bar charts represent mean ± SD. # Indicates a separator and has no practical meaning. *p < 0.05, **p < 0.01, ****p < 0.0001.
Techniques Used: Expressing, Quantitative RT-PCR, Knockdown, Over Expression, Western Blot, Control, Immunofluorescence, Staining, Transfection
Figure Legend Snippet: Levels of YAP1 protein in NUP205 knockdown HCC cells. ( A and B ) HCCLM3 ( A ) and SK-Hep1 ( B ) cells were transfected with siNUP205 and treated with 30 μg/mL CHX for 0, 6, 12 and 24 h. Western blotting images showing levels of YAP1 protein are shown on the left with quantitative analysis on the right; ( C and D ) HCC LM3 ( C ) and SK-Hep1 ( D ) cells were transfected with siNUP205 and treated with 10 μM MG132 for 12 h or 25 μM CQ for 12 h (n = 3). Western blotting images with levels of NUP205 and YAP1 are shown in the upper panels with quantitative analysis in the lower panels; ( E ) Immunoprecipitation assays to show levels of ubiquitylated YAP1 protein in NUP205 silenced SK-Hep1 cells. Bars represent mean ± SD. # Indicates a separator and has no practical meaning. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns = not significant by one-way ANOVA with multiple comparison.
Techniques Used: Knockdown, Transfection, Western Blot, Immunoprecipitation, Comparison
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