Journal: Nature Communications
Article Title: DNA-PK-mediated phosphorylation of STAT6 establishes a non-canonical type 2 immunity axis to prevent macrophage senescence
doi: 10.1038/s41467-026-69996-8
Figure Lengend Snippet: A Immunoblots of pSTAT6 (pY641) and γH2AX in thioglycolate-elicited peritoneal macrophages (Thio-PM) with Etoposide (Eto) and IL-4 treatment. The right panel shows the relative intensity of pSTAT6 (pY641) ( n = 4 independent experiments). B Immunofluorescence images of staining (pSTAT6 (pY641), red; DAPI, blue) of IL-4-stimulated Thio-PMs treated with Eto. Scale bars 50 μm. Right panel shows the quantification of pSTAT6 (pY641) ( n = 3 biological replicates per group). C Immunoblots of pSTAT6 (pY641) and γH2AX in Eto-treated Thio-PMs with different inhibitors. KU55933, an inhibitor of ATM. Berzosertib, inhibitor of ATR. NU7026, inhibitor of DNA-PK. AZD7762, inhibitor of CHK1/2. The right panel shows the quantification of pSTAT6 (pY641) ( n = 3 independent experiments). D Immunoblots of pSTAT6 (pY641) and γH2AX in Eto-treated Cas9 tg/+ Thio-PMs with indicated sgRNA. The right panel shows the quantification of pSTAT6 (pY641)/tSTAT6 ( n = 3 independent experiments). E Dual luciferase reporter of STAT6 in RAW 264.7 cells treated with Eto and NU7026 ( n = 3 per group). Data are mean ± s.e.m. p-value was calculated by paired two-tailed Student’s t test ( A , D ), unpaired two-tailed Student’s t test ( B ), one-way ANOVA with Dunnett’s correction ( C , E ).
Article Snippet: IL-4 (20 ng/mL, PeproTech, #214-14), DNA-PK inhibitor NU7026 (10 μM, Selleck Chemicals, #S2893), ATM inhibitor KU55933 (10 μM, Selleck Chemicals, #S1092), ATR inhibitor Berzosertib (0.5 μM, Selleck Chemicals, #S7102), CHK1/2 inhibitor AZD7762 (1 μM, Selleck Chemicals, #S1532), STAT6 inhibitor AS1517499 (2 μM, Selleck Chemicals, #S8685), STING inhibitor H151 (1 μM, Selleck Chemicals, #S6652), PTP1B inhibitor (2 μM, Santa Cruz, #sc-222227), PU.1 inhibitor DB2313 (10 nM, MedChem Express, #HY-124629), EPAC inhibitor ESI-09 (10 μM, Selleck Chemicals, #S7499), hIL-4 (20 ng/mL, PeproTech, #200-04), LPS (100 ng/mL, Sigma-Aldrich, #L2630), IFNγ (20 ng/mL, PeproTech, #315-05) were used.
Techniques: Western Blot, Immunofluorescence, Staining, Luciferase, Two Tailed Test
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