Journal: International Journal of Oncology
Article Title: Long non-coding RNA ABHD11-AS1 inhibits colorectal cancer progression through interacting with EGFR to suppress the EGFR/ERK signaling pathway
doi: 10.3892/ijo.2025.5726
Figure Lengend Snippet: Induction of EGFR overexpression abrogates ABHD11-AS1-mediated attenuation of proliferative and migratory behaviors in CRC cells. ABHD11-AS1 was overexpressed or knocked down in HCT116 and SW620 cells, respectively, and NSC228155 and chrysophanol were administered. (A) Western blot analysis of EGFR, ERK1/2, and EGFR and ERK1/2 phosphorylation in (A) HCT116 cells. (B) Western blot analysis of EGFR, ERK1/2, and EGFR and (B) SW620 cells. (C) Effects of NSC228155 on colony formation capacities in HCT116 cells. (D) Effects of chrysophanol on colony formation capacities in SW620 cells. (E) Statistical graphs of colony formation assays in HCT116 cells. (F) Statistical graphs of colony formation assays in SW620 cells. (G) Effects of NSC228155 on migration and invasion capacities of HCT116 cells. (H) Effects of chrysophanol on migration and invasion capacities of SW620 cells. (I) Statistical graphs of Transwell migration assays in HCT116 cells. (J) Statistical graphs of Transwell invasion assays in HCT116 cells. (K) Migration assays in SW620 cells. (L) Statistical graphs of Transwell invasion assays in SW620 cells. Scale bar, 50 μ m. ** P<0.01, *** P<0.001. CRC, colorectal cancer; p-, phosphorylated; ns, not significant; sh, short hairpin.
Article Snippet: For NSC228155 (cat. no. S8312, Selleck, Inc.), an EGFR activator, cells were treated with 100 μ M NSC228155 in humidified air with 5% CO 2 at 37°C for 12 h and then used for subsequent experiments.
Techniques: Over Expression, Western Blot, Phospho-proteomics, Migration
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