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mab377 ab 2298772 mouse anti coup tf2 nr2f2 coup tf ii nr2f2  (R&D Systems)


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    R&D Systems mab377 ab 2298772 mouse anti coup tf2 nr2f2 coup tf ii nr2f2
    Mab377 Ab 2298772 Mouse Anti Coup Tf2 Nr2f2 Coup Tf Ii Nr2f2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nrg1/Human+Neuregulin-1%2FNRG1+Antibody/pm41936722-73-32-47
    Average 94 stars, based on 53 article reviews
    mab377 ab 2298772 mouse anti coup tf2 nr2f2 coup tf ii nr2f2 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Modification:

    Article Title: PMP2 Enhances Schwann Cell Metabolism and Promotes Myelination.
    Article Snippet: .. Cells were maintained in media containing high glucose Dulbecco's Modified Eagle Medium (DMEM) (4.5 g/L glucose) supplemented with 10% fetal bovine serum, 2 mM L- glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, 10 ng/mL Nrg1 (human NRG1- β1 extracellular domain, R&D Systems Cat. No. 377- HB), and 2 μM forskolin. .. After 7 days, cells were dissociated enzymatically using a mixture of 2.5 mg/mL of dispase II (SigmaAldrich) and 130 U/mL of type I collagenase (Worthington Biochemical Corporation) for 3 h at 37°C, mechanically dissociated using fire- polished glass pipettes, and seeded on one 35 mm dish coated with laminin (Sigma- Aldrich).

    Staining:

    Article Title: Dickkopf 1 and Neuregulin 1 as cutaneous biomarkers for dermal pigmentation activity in acquired dermal macular hyperpigmentation (ADMH): a case control study.
    Article Snippet: Melanocytes are significantly influenced by growth factors released by keratinocytes and dermal fibroblasts [4].. Dickkopf1 (DKK1) secreted by dermal fibroblasts inhibits development of melanocytes and also plays an inhibitory role in the transfer of melanosomes [4].. Neuregulin 1 (NRG1) is a fibroblast-derived neuroendocrine factor which helps to regulate pigmentation by increasing the number as well as size of melanocytes [5].

    Incubation:

    Article Title: Dickkopf 1 and Neuregulin 1 as cutaneous biomarkers for dermal pigmentation activity in acquired dermal macular hyperpigmentation (ADMH): a case control study.
    Article Snippet: Melanocytes are significantly influenced by growth factors released by keratinocytes and dermal fibroblasts [4].. Dickkopf1 (DKK1) secreted by dermal fibroblasts inhibits development of melanocytes and also plays an inhibitory role in the transfer of melanosomes [4].. Neuregulin 1 (NRG1) is a fibroblast-derived neuroendocrine factor which helps to regulate pigmentation by increasing the number as well as size of melanocytes [5].

    Cell Culture:

    Article Title: Therapeutic effects of conditioned medium of immortalized dental pulp stem cells from human exfoliated deciduous teeth against experimental autoimmune neuritis
    Article Snippet: IMS32 cells (1 × 10 3 cells/200 μL) were cultured in serum-free DMEM containing SHED-CM or control medium (0, 1, 3, 10, 30%) for 72 h, and cell proliferative activity was determined using CellTiter-Glo 2.0 Cell Viability Assay (Promega) and measured with GloMax Discovery Microplate Reader (Promega) according to the manufacturer’s instructions. .. To examine the effects of HGF, NRG1 and bFGF in SHED-CM on the cell proliferation, IMS32 cells were similarly cultured with SHED-CM (30%) that had been preincubated with neutralizing antibodies against HGF (Clone # 24612, R&D Systems) or NRG1 (Catalog # AF-396-NA, R&D Systems) or bFGF (Clone # BHFB15, Biolegend) or respective control antibodies for 30 min, and cell proliferative activity was determined as described above. .. SHED-CM was separated into two fractions by ultracentrifugation using the Beckman Optima MAX-XP Ultracentrifuge at 186,000 × g for 120 min at 4°C with TLA-55 rotor (Beckman Coulter): the upper fraction contained SHED-CM without exosomes and the lower fraction contained precipitated exosomes from SHED-CM as described .

    Article Title: Therapeutic effects of conditioned medium of immortalized dental pulp stem cells from human exfoliated deciduous teeth against experimental autoimmune neuritis.
    Article Snippet: IMS32 cells (1 × 103 cells/200 μL) were cultured in serum-free DMEM containing SHED-CM or control medium (0, 1, 3, 10, 30%) for 72 h, and cell proliferative activity was determined using CellTiter-Glo 2.0 Cell Viability Assay (Promega) and measured with GloMax Discovery Microplate Reader (Promega) according to the manufacturer’s instructions. .. To examine the effects of HGF, NRG1 and bFGF in SHED-CM on the cell proliferation, IMS32 cells were similarly cultured with SHED-CM (30%) that had been preincubated with neutralizing antibodies against HGF (Clone # 24612, R&D Systems) or NRG1 (Catalog # AF-396-NA, R&D Systems) or bFGF (Clone # BHFB15, Biolegend) or respective control antibodies for 30 min, and cell proliferative activity was determined as described above. .. SHED-CM was separated into two fractions by ultracentrifugation using the Beckman Optima MAX-XP Ultracentrifuge at 186,000 × g for 120 min at 4°C with TLA-55 rotor (Beckman Coulter): the upper fraction contained SHED-CM without exosomes and the lower fraction contained precipitated exosomes from SHED-CM as described28.

    Control:

    Article Title: Therapeutic effects of conditioned medium of immortalized dental pulp stem cells from human exfoliated deciduous teeth against experimental autoimmune neuritis
    Article Snippet: IMS32 cells (1 × 10 3 cells/200 μL) were cultured in serum-free DMEM containing SHED-CM or control medium (0, 1, 3, 10, 30%) for 72 h, and cell proliferative activity was determined using CellTiter-Glo 2.0 Cell Viability Assay (Promega) and measured with GloMax Discovery Microplate Reader (Promega) according to the manufacturer’s instructions. .. To examine the effects of HGF, NRG1 and bFGF in SHED-CM on the cell proliferation, IMS32 cells were similarly cultured with SHED-CM (30%) that had been preincubated with neutralizing antibodies against HGF (Clone # 24612, R&D Systems) or NRG1 (Catalog # AF-396-NA, R&D Systems) or bFGF (Clone # BHFB15, Biolegend) or respective control antibodies for 30 min, and cell proliferative activity was determined as described above. .. SHED-CM was separated into two fractions by ultracentrifugation using the Beckman Optima MAX-XP Ultracentrifuge at 186,000 × g for 120 min at 4°C with TLA-55 rotor (Beckman Coulter): the upper fraction contained SHED-CM without exosomes and the lower fraction contained precipitated exosomes from SHED-CM as described .

    Article Title: Therapeutic effects of conditioned medium of immortalized dental pulp stem cells from human exfoliated deciduous teeth against experimental autoimmune neuritis.
    Article Snippet: IMS32 cells (1 × 103 cells/200 μL) were cultured in serum-free DMEM containing SHED-CM or control medium (0, 1, 3, 10, 30%) for 72 h, and cell proliferative activity was determined using CellTiter-Glo 2.0 Cell Viability Assay (Promega) and measured with GloMax Discovery Microplate Reader (Promega) according to the manufacturer’s instructions. .. To examine the effects of HGF, NRG1 and bFGF in SHED-CM on the cell proliferation, IMS32 cells were similarly cultured with SHED-CM (30%) that had been preincubated with neutralizing antibodies against HGF (Clone # 24612, R&D Systems) or NRG1 (Catalog # AF-396-NA, R&D Systems) or bFGF (Clone # BHFB15, Biolegend) or respective control antibodies for 30 min, and cell proliferative activity was determined as described above. .. SHED-CM was separated into two fractions by ultracentrifugation using the Beckman Optima MAX-XP Ultracentrifuge at 186,000 × g for 120 min at 4°C with TLA-55 rotor (Beckman Coulter): the upper fraction contained SHED-CM without exosomes and the lower fraction contained precipitated exosomes from SHED-CM as described28.

    Activity Assay:

    Article Title: Therapeutic effects of conditioned medium of immortalized dental pulp stem cells from human exfoliated deciduous teeth against experimental autoimmune neuritis
    Article Snippet: IMS32 cells (1 × 10 3 cells/200 μL) were cultured in serum-free DMEM containing SHED-CM or control medium (0, 1, 3, 10, 30%) for 72 h, and cell proliferative activity was determined using CellTiter-Glo 2.0 Cell Viability Assay (Promega) and measured with GloMax Discovery Microplate Reader (Promega) according to the manufacturer’s instructions. .. To examine the effects of HGF, NRG1 and bFGF in SHED-CM on the cell proliferation, IMS32 cells were similarly cultured with SHED-CM (30%) that had been preincubated with neutralizing antibodies against HGF (Clone # 24612, R&D Systems) or NRG1 (Catalog # AF-396-NA, R&D Systems) or bFGF (Clone # BHFB15, Biolegend) or respective control antibodies for 30 min, and cell proliferative activity was determined as described above. .. SHED-CM was separated into two fractions by ultracentrifugation using the Beckman Optima MAX-XP Ultracentrifuge at 186,000 × g for 120 min at 4°C with TLA-55 rotor (Beckman Coulter): the upper fraction contained SHED-CM without exosomes and the lower fraction contained precipitated exosomes from SHED-CM as described .

    Article Title: Therapeutic effects of conditioned medium of immortalized dental pulp stem cells from human exfoliated deciduous teeth against experimental autoimmune neuritis.
    Article Snippet: IMS32 cells (1 × 103 cells/200 μL) were cultured in serum-free DMEM containing SHED-CM or control medium (0, 1, 3, 10, 30%) for 72 h, and cell proliferative activity was determined using CellTiter-Glo 2.0 Cell Viability Assay (Promega) and measured with GloMax Discovery Microplate Reader (Promega) according to the manufacturer’s instructions. .. To examine the effects of HGF, NRG1 and bFGF in SHED-CM on the cell proliferation, IMS32 cells were similarly cultured with SHED-CM (30%) that had been preincubated with neutralizing antibodies against HGF (Clone # 24612, R&D Systems) or NRG1 (Catalog # AF-396-NA, R&D Systems) or bFGF (Clone # BHFB15, Biolegend) or respective control antibodies for 30 min, and cell proliferative activity was determined as described above. .. SHED-CM was separated into two fractions by ultracentrifugation using the Beckman Optima MAX-XP Ultracentrifuge at 186,000 × g for 120 min at 4°C with TLA-55 rotor (Beckman Coulter): the upper fraction contained SHED-CM without exosomes and the lower fraction contained precipitated exosomes from SHED-CM as described28.

    Recombinant:

    Article Title: HER4 Affects Sensitivity to Tamoxifen and Abemaciclib in Luminal Breast Cancer Cells and Restricts Tumor Growth in MCF-7-Based Humanized Tumor Mice.
    Article Snippet: ER/HER4 double positive human BC cell lines MCF-7 (American Type Culture Collection no. HTB22TM, RRID:CVCL_0031), T-47D (ATCC no. HTB-133TM, RRID: CVCL_0553) and ZR-75-1 (ATCC no. CRL-1500TM, RRID:CVCL_0588) were cultivated in DMEM (MCF-7, T-47D) or RPMI (ZR-75-1) supplemented with 5% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA) at 37 ◦C and 5% CO2. .. For short-term treatments, BC cell lines were treated with 10 nM 17-β-estradiol (SigmaAldrich, St. Louis, MO, USA), 5 μM (Z)-4-Hydroxytamoxifen (Sigma-Aldrich), 30 ng/mL NRG1 (Recombinant Human NRG1-beta 1/HRG1-beta 1 ECD Protein, R&D Systems, Minneapolis, MN, USA), 100 nM abemaciclib (Selleck Chemicals, Cologne, Germany), 100 nM AMG232 (Axon Medchem LLC, Reston, VA, USA) and various combinations thereof. ..

    Article Title: HER4 Affects Sensitivity to Tamoxifen and Abemaciclib in Luminal Breast Cancer Cells and Restricts Tumor Growth in MCF-7-Based Humanized Tumor Mice
    Article Snippet: ER/HER4 double positive human BC cell lines MCF-7 (American Type Culture Collection no. HTB-22TM, RRID:CVCL_0031), T-47D (ATCC no. HTB-133TM, RRID: CVCL_0553) and ZR-75-1 (ATCC no. CRL-1500TM, RRID:CVCL_0588) were cultivated in DMEM (MCF-7, T-47D) or RPMI (ZR-75-1) supplemented with 5% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C and 5% CO 2 . .. For short-term treatments, BC cell lines were treated with 10 nM 17-β-estradiol (Sigma-Aldrich, St. Louis, MO, USA), 5 μM (Z)-4-Hydroxytamoxifen (Sigma-Aldrich), 30 ng/mL NRG1 (Recombinant Human NRG1-beta 1/HRG1-beta 1 ECD Protein, R&D Systems, Minneapolis, MN, USA), 100 nM abemaciclib (Selleck Chemicals, Cologne, Germany), 100 nM AMG232 (Axon Medchem LLC, Reston, VA, USA) and various combinations thereof. ..



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    NRG1 expression is elevated in RAW264.7 macrophages co-cultured with 4T1 cells. (A) The expression of NRG1 was examined using next-generation RNA sequencing analysis. (B) The mRNA expression of NRG1 in RAW264.7 macrophages co-cultured with 4T1 cells was detected using reverse transcription-quantitative PCR. (C) The protein expression of NRG1 in RAW264.7 macrophages co-cultured with 4T1 cells was detected using immunoblotting analysis. *** P<0.001. NRG1, neuregulin 1.

    Journal: International Journal of Oncology

    Article Title: Hypoxia-induced exosomal CAMTA1 promotes radio-resistance in MDA-MB-231 cells by regulating NRG1 to mediate M2 macrophage polarization

    doi: 10.3892/ijo.2026.5875

    Figure Lengend Snippet: NRG1 expression is elevated in RAW264.7 macrophages co-cultured with 4T1 cells. (A) The expression of NRG1 was examined using next-generation RNA sequencing analysis. (B) The mRNA expression of NRG1 in RAW264.7 macrophages co-cultured with 4T1 cells was detected using reverse transcription-quantitative PCR. (C) The protein expression of NRG1 in RAW264.7 macrophages co-cultured with 4T1 cells was detected using immunoblotting analysis. *** P<0.001. NRG1, neuregulin 1.

    Article Snippet: Blocked by 5% BSA (MilliporeSigma) for 1 h at room temperature, the membranes were incubated with primary antibodies against NRG1 (cat. no. 10527-1-AP; 1:1,000; Proteintech Group, Inc.), hypoxia-inducible factor-1α (HIF-1α; cat. no. 20960-1-AP; 1:2,000; Proteintech Group, Inc.), CD63 (cat. no. 25682-1-AP; 1:2,000; Proteintech Group, Inc.), CD81 (cat. no. 27855-1-AP; 1:1,000; Proteintech Group, Inc.), CD9 (cat. no. 20597-1-AP; 1:2,000; Proteintech Group, Inc.), ALIX (cat. no. 12422-1-AP; 1:5,000; Proteintech Group, Inc.) and GAPDH (cat. no. 10494-1-AP; 1:5,000; Proteintech Group, Inc.) overnight at 4°C.

    Techniques: Expressing, Cell Culture, RNA Sequencing, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot

    NRG1 expression is associated with M2 polarization and poor prognosis. (A) The expression of NRG1 in BC samples using TCGA database. (B) Spearman correlation analysis between NRG1 and CD163 expression in breast cancer samples. (C) The association of NRG1 expression with the overall survival in BC patients. NRG1, neuregulin 1; BC, breast cancer; TCGA, The Cancer Genome Atlas; GEPIA, Gene Expression Profiling Interactive Analysis.

    Journal: International Journal of Oncology

    Article Title: Hypoxia-induced exosomal CAMTA1 promotes radio-resistance in MDA-MB-231 cells by regulating NRG1 to mediate M2 macrophage polarization

    doi: 10.3892/ijo.2026.5875

    Figure Lengend Snippet: NRG1 expression is associated with M2 polarization and poor prognosis. (A) The expression of NRG1 in BC samples using TCGA database. (B) Spearman correlation analysis between NRG1 and CD163 expression in breast cancer samples. (C) The association of NRG1 expression with the overall survival in BC patients. NRG1, neuregulin 1; BC, breast cancer; TCGA, The Cancer Genome Atlas; GEPIA, Gene Expression Profiling Interactive Analysis.

    Article Snippet: Blocked by 5% BSA (MilliporeSigma) for 1 h at room temperature, the membranes were incubated with primary antibodies against NRG1 (cat. no. 10527-1-AP; 1:1,000; Proteintech Group, Inc.), hypoxia-inducible factor-1α (HIF-1α; cat. no. 20960-1-AP; 1:2,000; Proteintech Group, Inc.), CD63 (cat. no. 25682-1-AP; 1:2,000; Proteintech Group, Inc.), CD81 (cat. no. 27855-1-AP; 1:1,000; Proteintech Group, Inc.), CD9 (cat. no. 20597-1-AP; 1:2,000; Proteintech Group, Inc.), ALIX (cat. no. 12422-1-AP; 1:5,000; Proteintech Group, Inc.) and GAPDH (cat. no. 10494-1-AP; 1:5,000; Proteintech Group, Inc.) overnight at 4°C.

    Techniques: Expressing, Gene Expression

    Hypoxic MDA-MB-231 cells induces the M2 polarization of THP-1 macrophages via NRG1. (A) The mRNA expression of NRG1 was detected using RT-qPCR. (B) The transfection efficacy of Ov-NRG1 was detected using RT-qPCR and immunoblotting analysis. (C) The transfection efficacy of si-NRG1 was detected using RT-qPCR and immunoblotting analysis. (D) The level of IL-10 was detected using ELISA-related IL-10 assay kits. (E and F) The level of CD163 was detected using flow cytometry. (G) Following the treatment of anti-NRG1 blocking antibody, the level of IL-10 was detected using ELISA-related IL-10 assay kits. (H-I) Following the treatment of anti-NRG1 blocking antibody, the level of CD163 was detected using flow cytometry. * P<0.05, ** P<0.01 and *** P<0.001. NRG1, neuregulin 1; RT-qPCR, reverse transcription-quantitative PCR; Ov, overexpression; si, small interfering.

    Journal: International Journal of Oncology

    Article Title: Hypoxia-induced exosomal CAMTA1 promotes radio-resistance in MDA-MB-231 cells by regulating NRG1 to mediate M2 macrophage polarization

    doi: 10.3892/ijo.2026.5875

    Figure Lengend Snippet: Hypoxic MDA-MB-231 cells induces the M2 polarization of THP-1 macrophages via NRG1. (A) The mRNA expression of NRG1 was detected using RT-qPCR. (B) The transfection efficacy of Ov-NRG1 was detected using RT-qPCR and immunoblotting analysis. (C) The transfection efficacy of si-NRG1 was detected using RT-qPCR and immunoblotting analysis. (D) The level of IL-10 was detected using ELISA-related IL-10 assay kits. (E and F) The level of CD163 was detected using flow cytometry. (G) Following the treatment of anti-NRG1 blocking antibody, the level of IL-10 was detected using ELISA-related IL-10 assay kits. (H-I) Following the treatment of anti-NRG1 blocking antibody, the level of CD163 was detected using flow cytometry. * P<0.05, ** P<0.01 and *** P<0.001. NRG1, neuregulin 1; RT-qPCR, reverse transcription-quantitative PCR; Ov, overexpression; si, small interfering.

    Article Snippet: Blocked by 5% BSA (MilliporeSigma) for 1 h at room temperature, the membranes were incubated with primary antibodies against NRG1 (cat. no. 10527-1-AP; 1:1,000; Proteintech Group, Inc.), hypoxia-inducible factor-1α (HIF-1α; cat. no. 20960-1-AP; 1:2,000; Proteintech Group, Inc.), CD63 (cat. no. 25682-1-AP; 1:2,000; Proteintech Group, Inc.), CD81 (cat. no. 27855-1-AP; 1:1,000; Proteintech Group, Inc.), CD9 (cat. no. 20597-1-AP; 1:2,000; Proteintech Group, Inc.), ALIX (cat. no. 12422-1-AP; 1:5,000; Proteintech Group, Inc.) and GAPDH (cat. no. 10494-1-AP; 1:5,000; Proteintech Group, Inc.) overnight at 4°C.

    Techniques: Expressing, Quantitative RT-PCR, Transfection, Western Blot, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Blocking Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, Over Expression

    Exosomal CAMTA1 promotes tumor growth in vivo . (A) The transfection efficacy of Ov-CAMTA1 was detected using RT-qPCR and immunoblotting analysis. (B) The appearance of tumor. The tumor (C) volume and (D) weight. (E) The mRNA expression of CAMTA1 was detected using RT-qPCR. (F) The level of IL-10 was detected using ELISA-related IL-10 assay kits. (G) The level of CD163 was detected using immunohistochemistry analysis. (H) H&E staining. (I) The expression of Caspase 3 was detected using immunohistochemistry analysis. (J) The Spearman correlation analysis of CAMTA1 and NRG1. (K) The expression of NRG1 was detected using immunohistochemistry analysis. * P<0.05, ** P<0.01 and *** P<0.001. CAMTA1, Calmodulin-binding Transcription Activator 1; Ov, overexpression; RT-qPCR, reverse transcription-quantitative PCR; H&E, hematoxylin and eosin; NRG1, neuregulin 1.

    Journal: International Journal of Oncology

    Article Title: Hypoxia-induced exosomal CAMTA1 promotes radio-resistance in MDA-MB-231 cells by regulating NRG1 to mediate M2 macrophage polarization

    doi: 10.3892/ijo.2026.5875

    Figure Lengend Snippet: Exosomal CAMTA1 promotes tumor growth in vivo . (A) The transfection efficacy of Ov-CAMTA1 was detected using RT-qPCR and immunoblotting analysis. (B) The appearance of tumor. The tumor (C) volume and (D) weight. (E) The mRNA expression of CAMTA1 was detected using RT-qPCR. (F) The level of IL-10 was detected using ELISA-related IL-10 assay kits. (G) The level of CD163 was detected using immunohistochemistry analysis. (H) H&E staining. (I) The expression of Caspase 3 was detected using immunohistochemistry analysis. (J) The Spearman correlation analysis of CAMTA1 and NRG1. (K) The expression of NRG1 was detected using immunohistochemistry analysis. * P<0.05, ** P<0.01 and *** P<0.001. CAMTA1, Calmodulin-binding Transcription Activator 1; Ov, overexpression; RT-qPCR, reverse transcription-quantitative PCR; H&E, hematoxylin and eosin; NRG1, neuregulin 1.

    Article Snippet: Blocked by 5% BSA (MilliporeSigma) for 1 h at room temperature, the membranes were incubated with primary antibodies against NRG1 (cat. no. 10527-1-AP; 1:1,000; Proteintech Group, Inc.), hypoxia-inducible factor-1α (HIF-1α; cat. no. 20960-1-AP; 1:2,000; Proteintech Group, Inc.), CD63 (cat. no. 25682-1-AP; 1:2,000; Proteintech Group, Inc.), CD81 (cat. no. 27855-1-AP; 1:1,000; Proteintech Group, Inc.), CD9 (cat. no. 20597-1-AP; 1:2,000; Proteintech Group, Inc.), ALIX (cat. no. 12422-1-AP; 1:5,000; Proteintech Group, Inc.) and GAPDH (cat. no. 10494-1-AP; 1:5,000; Proteintech Group, Inc.) overnight at 4°C.

    Techniques: In Vivo, Transfection, Quantitative RT-PCR, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Staining, Binding Assay, Over Expression, Reverse Transcription, Real-time Polymerase Chain Reaction