Journal: Kidney international

Article Title: The L1 cell adhesion molecule is a potential biomarker of human distal nephron injury in acute tubular necrosis.

doi: 10.1038/sj.ki.5002640

Figure Lengend Snippet: Figure 4 | Comparative localization of L1 intra- and extracytoplasmic domains. (a) L1 scheme showing the extracellular part with six immunoglobulin-like domains (Ig-like) (including one recognized by mAb272) and five fibronectin III-like (FNIII) domains followed by the transmembrane segment and cytoplasmic fragments (recognized by the goat polyclonal serum). (b–d) Normal CDs are compared with (e–g) damaged CDs. (b, e) CDs are stained by goat polyclonal antiserum labeled by FITC (green) and (c, f) mAb272 labeled by rhodamine (red). (d, g) Merge. (d) In normal CD, both antibodies give a basolateral signal. (g) In injured principal cells, the basolateral signal is low with both antibodies, whereas there is a strong apical membrane (arrow) signal with the anti-cytoplasmic domain antibody contrasting with no signal for the extracellular fragment. Original magnification 630.

Article Snippet: After four washes with TBS-T (Tris-buffered saline with 0.1% Tween), goat anti-human L1 extracellular domain antibody was added (R&D Systems), followed by anti-goat coupled to alkaline phosphatase (Sigma-Aldrich, St Louis, MO, USA).

Techniques: Staining, Labeling, Membrane

Journal: Nature neuroscience

Article Title: A glycolytic shift in Schwann cells supports injured axons

doi: 10.1038/s41593-020-0689-4

Figure Lengend Snippet: a, Top: Scheme of metabolomic analysis using extracts from nerve segments. Bottom: Concentrations of key energy metabolism intermediates in control and axotomized nerve segments from C57Bl/6J mice (F6P/G6P: fructose-6-phosphate/glucose-6-phosphate. FBP: fructose-1,6-bisphosphate. GI-OH3P: Glyceraldehyde-3-phosphate. 2PG/3PG: 2-phosphoglycerate/3-phosphoglycerate. LACT: lactate) (Error bars represent s.e.m. n=5 mice per condition and metabolite). b, Top: Scheme of extracellular flux analysis of SCs purified from C57Bl/6J mouse nerves. Bottom: Box and whiskers plot (maximum, 25th percentile, median, 75th percentile, minimum) shows glycolytic activity parameters as assessed by extracellular acidification rate (ECAR) measurements in control mouse SCs and cells with Nrg1-induced ErbB2 activation (n=9 well preparations per condition, *P=0.0080, **P=0.0464, ***P<0.0001). c, Western blot analysis (cropped blot images) of control- and Nrg1-treated C57Bl/6J mouse SCs probed with the indicated antibodies. (n=6 independent pair preparations (2 separate dishes) for PFKFB3, and n=3 independent pair preparations (2 separate dishes) for LDHA quantification). d, Intracellular and extracellular (supernatant) lactate concentrations from control and Nrg1-treated mouse SC preparations normalized to cell number and cellular protein. Note decreased intracellular and increased extracellular lactate levels in SCs treated with Nrg1 for 24h, indicating greatly increased lactate extrusion (Error bars represent s.e.m. n=3 well preparations from 3 independent experiments per condition).

Article Snippet: SCs were subsequently control-treated or treated with 200 ng/ml recombinant Nrg1 (R&D Systems, 396-HB-050) for 24h, collected in RIPA buffer containing phosphatase and protease inhibitors, and then processed for protein analysis and western blotting using standard procedures.

Techniques: Control, Purification, Activity Assay, Activation Assay, Western Blot

Journal: bioRxiv

Article Title: Neuregulin-1 protects against respiratory viral induced mortality

doi: 10.1101/2023.05.10.540232

Figure Lengend Snippet: ( A ) CD11c + cells are increased in the lungs of atopic mice. Flow cytometry of lung cell suspension showing frequency (left) and cell number (right) at day 3 post inoculation (PI) high dose SeV ( B ) Adoptive transfer of CD11c + cells isolated from NA or atopic mice into naïve mice 24 h before inoculation with high dose SeV delays but does not prevent mortality; n=4 per group. ( C ) Transcriptomic (RNAseq) comparison between FACS isolated lung CD11c + cells from atopic and NA mice identifies several disparately expressed gene products, including Nrg1 (n=4 per group). Selected gene products shown. ( D ) NRG1 is markedly increased in atopic mouse lung (“tissue”), BAL, and supernatant from 1 x10 6 atopic lung CD11c + cells (“CD11c sup”) cultured for 24 h. ( E ) The scRNA (10x Platform) tSNE coordinates show CD14 + monocytes and dendritic cell (“DC”) sub-populations expressing NRG1 in peripheral blood cells of healthy human donors.*p<0.05, **p<0.01, ****p<0.0001

Article Snippet: Cell culture basal media was supplemented with recombinant human NRG1 alpha (cat#: NBP2-35093, Novus Bio) for hBEC or with mouse NRG1 for mTEC on day five and three before and at the time of infection with 4000 pfu of rgRSV or SeV-GFP.

Techniques: Flow Cytometry, Suspension, Adoptive Transfer Assay, Isolation, Comparison, Cell Culture, Expressing

Journal: bioRxiv

Article Title: Neuregulin-1 protects against respiratory viral induced mortality

doi: 10.1101/2023.05.10.540232

Figure Lengend Snippet: ( A ) NRG1(1ng to 1000ng) i.n. (in 30µL) given daily to naïve mice for 5d before inoculation with high dose SeV reduces viral mortality; n=4 per group (1ng, 10ng 1000ng & PBS), n=8 per group (100 ng and 500 ng). ( B ) Ratio of EBD in the BAL to that in the lung shows reduced EBD in NRG1 treated mice on day 8 PI SeV. n≥8 per group, median ± IQR shown, Mann-Whitney U test.

Article Snippet: Cell culture basal media was supplemented with recombinant human NRG1 alpha (cat#: NBP2-35093, Novus Bio) for hBEC or with mouse NRG1 for mTEC on day five and three before and at the time of infection with 4000 pfu of rgRSV or SeV-GFP.

Techniques: MANN-WHITNEY

Journal: bioRxiv

Article Title: Neuregulin-1 protects against respiratory viral induced mortality

doi: 10.1101/2023.05.10.540232

Figure Lengend Snippet: ( A ) Adding NRG1 to hBEC inoculated with rgRSV (left) and mTEC inoculated with GFP-SeV (right) reduces spread of infection. Representative images shown. ( B ) Quantification of (A) for rgRSV and hBEC and ( C ) for GFP-SeV and mTEC. GFP positive cells quantified by ImageJ. Representative images from ≥3 separate experiments. *p<0.05, **p<0.01. ( D ) Transcriptomic analysis of hBEC cultures treated with NRG1 (100 ng) on the basolateral side of the Transwell for 5 days and inoculated with RSV (4000 pfu). RNA was isolated 48 h PI RSV and qRT-PCR performed using a custom Prime PCR array plate: (i) Transcripts in which NRG1 treatment reduced gene expression from that seen in RSV infected cells. (ii) Transcripts with low level expression that show small but significant change in expression relative to naïve control with NRG1 alone or genes significantly increased with RSV but whose expression levels were not affected by NRG1. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, n=3.

Article Snippet: Cell culture basal media was supplemented with recombinant human NRG1 alpha (cat#: NBP2-35093, Novus Bio) for hBEC or with mouse NRG1 for mTEC on day five and three before and at the time of infection with 4000 pfu of rgRSV or SeV-GFP.

Techniques: Infection, Isolation, Quantitative RT-PCR, Gene Expression, Expressing, Control

Journal: PLoS ONE

Article Title: Combining Anti-ERBB3 Antibodies Specific for Domain I and Domain III Enhances the Anti-Tumor Activity over the Individual Monoclonal Antibodies

doi: 10.1371/journal.pone.0112376

Figure Lengend Snippet: A) Serum-starved BT-474, NCI-N87 and ACHN cells were treated with appropriate antibodies or PBS for 30 minutes followed by HRGβ stimulation for 10 minutes. B) NCI-N87 cells were treated with either the A5/F4 oligoclonal or PBS and stimulated with HRGβ, as appropriate, over a 60 minute time course. Cell lysates were analyzed by western blot and probed with anti-pAKT(S473) as a marker for ligand-induced signaling. Membranes were probed with anti-beta actin mAb that served as loading control.

Article Snippet: After overnight serum starvation in 0.1–0.5% serum containing media, cells were pretreated with appropriate antibodies or PBS for 30 minutes and stimulated with 20 nM HRG-beta (R&D systems #377-HB-050) for indicated times.

Techniques: Western Blot, Marker, Control

Journal: BMC Cancer

Article Title: Unveiling GDF15 as a promising biomarker for predicting survival in pancreatic ductal carcinoma: a retrospective research

doi: 10.1186/s12885-025-14963-7

Figure Lengend Snippet: IHC results of GDF15 level in PDAC and normal pancreas tissues. a Immunohistochemical analysis revealed markedly elevated GDF15 expression in pancreatic ductal adenocarcinoma (PDAC) specimens, whereas adjacent non‑neoplastic pancreatic tissues showed little to no detectable GDF15 staining. b Patients with poor prognoses exhibited higher expression levels compared to those with more favorable outcomes. c H‑score results for GDF15 expression were graphically represented, revealing a statistically significant difference in staining intensity between PDAC tumor tissues and their adjacent non‑neoplastic counterparts

Article Snippet: The TMAs were prepared using a PV-9000 IHC kit (ZSGB-BIO, Beijing, China) and incubated with an anti-GDF15 antibody (27455-1-AP; Proteintech, Wuhan, China).

Techniques: Immunohistochemical staining, Expressing, Staining

Journal: BMC Cancer

Article Title: Unveiling GDF15 as a promising biomarker for predicting survival in pancreatic ductal carcinoma: a retrospective research

doi: 10.1186/s12885-025-14963-7

Figure Lengend Snippet: OS of PDAC patients with different GDF15 levels. Patients exhibiting low GDF15 expression showed significantly longer overall survival than those with high expression, as demonstrated by Kaplan–Meier analysis.

Article Snippet: The TMAs were prepared using a PV-9000 IHC kit (ZSGB-BIO, Beijing, China) and incubated with an anti-GDF15 antibody (27455-1-AP; Proteintech, Wuhan, China).

Techniques: Expressing