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Proteintech anti mr
Anti Mr, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nr3c2/NR3C2+Polyclonal+antibody/pm41579728-90-23-26
Average 93 stars, based on 40 article reviews
anti mr - by Bioz Stars, 2026-09
93/100 stars

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Related Articles

Western Blot:

Article Title: Effect and underlying mechanism of Huangjing Qianshi decoction in pre-diabetes mouse model.
Article Snippet: .. Antibodies against GLUT-4 (1:100 dilution, 66846-1-Ig), INS (1:1000 dilution, 66198-1-Ig), NR3C2 (1:2000 dilution for western blot and 1:100 dilution for IHC, 21854-1-AP), PIAS1 (1:2000 dilution for western blot and 1:100 dilution for IHC, 23395-1-AP), STAT1 (1:2000 dilution, 66545-1- Ig), p-STAT1 (1:2000 dilution, 28977-1-AP), PGC-1a (1:5000 dilution, 66369-1-Ig), and GAPDH (1:20000 dilution, 60004-1-Ig) were purchased from the Proteintech (Wuhan Sanying, Wuhan, China). .. All statistical analyses were performed using the Statistical Package for Social Sciences (SPSS) version 19.0 (IBM Inc.).

Article Title: Effect and underlying mechanism of Huangjing Qianshi decoction in pre-diabetes mouse model
Article Snippet: .. Antibodies against GLUT-4 (1:100 dilution, 66846-1-Ig), INS (1:1000 dilution, 66198-1-Ig), NR3C2 (1:2000 dilution for western blot and 1:100 dilution for IHC, 21854-1-AP), PIAS1 (1:2000 dilution for western blot and 1:100 dilution for IHC, 23395-1-AP), STAT1 (1:2000 dilution, 66545-1-Ig), p-STAT1 (1:2000 dilution, 28977-1-AP), PGC-1a (1:5000 dilution, 66369-1-Ig), and GAPDH (1:20000 dilution, 60004-1-Ig) were purchased from the Proteintech (Wuhan Sanying, Wuhan, China). .. All statistical analyses were performed using the Statistical Package for Social Sciences (SPSS) version 19.0 (IBM Inc.).

Immunohistochemistry:

Article Title: Effect and underlying mechanism of Huangjing Qianshi decoction in pre-diabetes mouse model.
Article Snippet: .. Antibodies against GLUT-4 (1:100 dilution, 66846-1-Ig), INS (1:1000 dilution, 66198-1-Ig), NR3C2 (1:2000 dilution for western blot and 1:100 dilution for IHC, 21854-1-AP), PIAS1 (1:2000 dilution for western blot and 1:100 dilution for IHC, 23395-1-AP), STAT1 (1:2000 dilution, 66545-1- Ig), p-STAT1 (1:2000 dilution, 28977-1-AP), PGC-1a (1:5000 dilution, 66369-1-Ig), and GAPDH (1:20000 dilution, 60004-1-Ig) were purchased from the Proteintech (Wuhan Sanying, Wuhan, China). .. All statistical analyses were performed using the Statistical Package for Social Sciences (SPSS) version 19.0 (IBM Inc.).

Article Title: Effect and underlying mechanism of Huangjing Qianshi decoction in pre-diabetes mouse model
Article Snippet: .. Antibodies against GLUT-4 (1:100 dilution, 66846-1-Ig), INS (1:1000 dilution, 66198-1-Ig), NR3C2 (1:2000 dilution for western blot and 1:100 dilution for IHC, 21854-1-AP), PIAS1 (1:2000 dilution for western blot and 1:100 dilution for IHC, 23395-1-AP), STAT1 (1:2000 dilution, 66545-1-Ig), p-STAT1 (1:2000 dilution, 28977-1-AP), PGC-1a (1:5000 dilution, 66369-1-Ig), and GAPDH (1:20000 dilution, 60004-1-Ig) were purchased from the Proteintech (Wuhan Sanying, Wuhan, China). .. All statistical analyses were performed using the Statistical Package for Social Sciences (SPSS) version 19.0 (IBM Inc.).

Pyrolysis Gas Chromatography:

Article Title: Effect and underlying mechanism of Huangjing Qianshi decoction in pre-diabetes mouse model.
Article Snippet: .. Antibodies against GLUT-4 (1:100 dilution, 66846-1-Ig), INS (1:1000 dilution, 66198-1-Ig), NR3C2 (1:2000 dilution for western blot and 1:100 dilution for IHC, 21854-1-AP), PIAS1 (1:2000 dilution for western blot and 1:100 dilution for IHC, 23395-1-AP), STAT1 (1:2000 dilution, 66545-1- Ig), p-STAT1 (1:2000 dilution, 28977-1-AP), PGC-1a (1:5000 dilution, 66369-1-Ig), and GAPDH (1:20000 dilution, 60004-1-Ig) were purchased from the Proteintech (Wuhan Sanying, Wuhan, China). .. All statistical analyses were performed using the Statistical Package for Social Sciences (SPSS) version 19.0 (IBM Inc.).

Article Title: Effect and underlying mechanism of Huangjing Qianshi decoction in pre-diabetes mouse model
Article Snippet: .. Antibodies against GLUT-4 (1:100 dilution, 66846-1-Ig), INS (1:1000 dilution, 66198-1-Ig), NR3C2 (1:2000 dilution for western blot and 1:100 dilution for IHC, 21854-1-AP), PIAS1 (1:2000 dilution for western blot and 1:100 dilution for IHC, 23395-1-AP), STAT1 (1:2000 dilution, 66545-1-Ig), p-STAT1 (1:2000 dilution, 28977-1-AP), PGC-1a (1:5000 dilution, 66369-1-Ig), and GAPDH (1:20000 dilution, 60004-1-Ig) were purchased from the Proteintech (Wuhan Sanying, Wuhan, China). .. All statistical analyses were performed using the Statistical Package for Social Sciences (SPSS) version 19.0 (IBM Inc.).

Incubation:

Article Title: Aldosterone induces renal lymphangiogenesis through macrophage-lymphatic endothelial cell transformation and Inhibition by esaxerenone.
Article Snippet: Haixia Guo, Ziqian Liu and Ruyan Lv have contributed equally to this work.. Abstract Objective and design Inflammation plays a crucial role in the occurrence and development of renal fibrosis.. Lymphatic vessels have emerged as new hotspots in the domain of inflammation.

Article Title: Aldosterone-Induced Renal Lymphangiogenesis and Endothelial–To–Mesenchymal Transformation to Promote Renal Interstitial Fibrosis Through the MR/TGF-β1 Pathway in Mice
Article Snippet: .. After blocking with 5% skim milk at room temperature for 2 h, the membranes were incubated with the following primary antibodies at 4 °C overnight: vimentin (1:1000, Abcam, Cat# ab8978), IL-1β (1:300, Abcam, Cat# ab283822), IL-1β (1:500, Affinity, Cat# AF5103), TGF-β1 (1:1000, Abcam, Cat# ab215715), VEGFR3 (1:500, Abcam, Cat# ab27278), α-SMA (1:1000, ABclonal, Wuhan, China, Cat# A17910), TNF-α (1:1000, ABclonal, Cat# A0277), podoplanin (1:1000, ABclonal, Cat# A13261), LYVE-1 (1:1000, ABclonal, A4352), VEGFC (1:1000, Immunoway, Cat# Y75297), and NR3C2 (1:300, Proteintech, Wuhan, China, Cat# 21854-1-AP). .. On the second day, the membrane was incubated with a secondary antibody (1:10,000) at room temperature for 1 h. Odyssey imaging system (LI-COR system, Lincoln, NE, USA) or e-BLOT system (Shanghai e-BLOT Photoelectrics Technology Co., Ltd., Shanghai, China) was used to detect the protein bands, determination of target protein bands with ImageJ software (National Institutes of Health) relative to the housekeeping protein such as GAPDH (1:1000, Affinity, Cat# T0004), βactin (1:1000, Affinity, Cat# T0022), β-Tubulin (1:1000, Affinity, Cat# T0023), or PCNA bands (1:1000, Proteintech, Cat# 10205-2-AP).

Article Title: Esaxerenone inhibits renal macrophage proliferation through MR/TGF-β1 pathway in db/db mice.
Article Snippet: .. The samples were subsequently blocked with 5% BSA or normal goat serum and incubated overnight at 4°C with primary antibodies against antigen F4/80 (1:200, Servicebio, Cat#: GB113373), Ki-67 (1:100, Abcam, Cat#: ab15580), NR3C2 (1:400, Proteintech, Cat#: 21854-1-AP), TGF-1 (1:200, Servicebio, Cat#: GB11179). .. After washing, the samples were incubated with secondary antibodies conjugated with Alexa Fluor ® 488 (1:300, Abcam, Cat#: ab150081) or Alexa Fluor ® 555 (1:300, Abcam, Cat#: ab150078) at 37 ◦C for 2h.

Article Title: Esaxerenone inhibits renal macrophage proliferation through MR/TGF-β1 pathway in db/db mice.
Article Snippet: .. Resolved proteins were electrophoretically transferred to PVDF membranes, and the membranes were blocked with 5% BSA and incubated overnight (4°C) with primary antibodies against IL-1 (1:1000, Zenbio, Cat#: 516288), TNF- (1:100, Servicebio, Cat#: GB11188), MCP-1 (1:500, Zenbio, Cat#: 507277), TGF-1 (1:1000, Affinity, Cat#: AF1027), ICAM-1 (1:1000, Proteintech, Cat#: 10020-1-AP), PCNA(1:1000, Proteintech, Cat#: 10205-2-AP), ERK1/2 (1:1000, Cell Signaling Technology, Cat#: 4652), phospho-ERK1/2 (1:1000, Cell Signaling Technology, Cat#: 4370) , NR3C2 (1:1000, Proteintech, Cat#: 21854–1-AP), Smad2/3 (1:1000, Affinity, Cat#: AF6367) and Smad7 (1:1000, Affinity, Cat#: AF5147). ..

Protein Extraction:

Article Title: Esaxerenone attenuates high salt-induced hypertension and renal damage via CD8 + T cell- associated NCC activation in aldosterone treated rats.
Article Snippet: Salt-water retention plays key roles in chronic kidney disease (CKD) and hypertension.. The mineralocorticoid receptor (MR) blocker esaxerenone could attenuate hypertension and renal injury; however, the mechanism is only partially understood.. In this study, we used rats that were fed 8 % high-salt diet or/and infused with aldosterone to explore the possible mechanism of salt-water retention in CKD and hypertension, and the protective effect of esaxerenone.

Blocking Assay:

Article Title: Aldosterone-Induced Renal Lymphangiogenesis and Endothelial–To–Mesenchymal Transformation to Promote Renal Interstitial Fibrosis Through the MR/TGF-β1 Pathway in Mice
Article Snippet: .. After blocking with 5% skim milk at room temperature for 2 h, the membranes were incubated with the following primary antibodies at 4 °C overnight: vimentin (1:1000, Abcam, Cat# ab8978), IL-1β (1:300, Abcam, Cat# ab283822), IL-1β (1:500, Affinity, Cat# AF5103), TGF-β1 (1:1000, Abcam, Cat# ab215715), VEGFR3 (1:500, Abcam, Cat# ab27278), α-SMA (1:1000, ABclonal, Wuhan, China, Cat# A17910), TNF-α (1:1000, ABclonal, Cat# A0277), podoplanin (1:1000, ABclonal, Cat# A13261), LYVE-1 (1:1000, ABclonal, A4352), VEGFC (1:1000, Immunoway, Cat# Y75297), and NR3C2 (1:300, Proteintech, Wuhan, China, Cat# 21854-1-AP). .. On the second day, the membrane was incubated with a secondary antibody (1:10,000) at room temperature for 1 h. Odyssey imaging system (LI-COR system, Lincoln, NE, USA) or e-BLOT system (Shanghai e-BLOT Photoelectrics Technology Co., Ltd., Shanghai, China) was used to detect the protein bands, determination of target protein bands with ImageJ software (National Institutes of Health) relative to the housekeeping protein such as GAPDH (1:1000, Affinity, Cat# T0004), βactin (1:1000, Affinity, Cat# T0022), β-Tubulin (1:1000, Affinity, Cat# T0023), or PCNA bands (1:1000, Proteintech, Cat# 10205-2-AP).



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Thermo Fisher gene exp nr3c2 rn00565562 m1
Effect of Ald on Na transport activity in proximal tubules (PTs) is mediated by the MR signaling pathway (A) <t>NR3C2</t> expression in the kidney and mRNA expression ratios in the glomeruli, proximal tubules (PTs), and other compartments isolated manually from fresh renal cortices. DT + CCD, distal tubule and cortical collecting duct. **p < 0.01 versus glomeruli; ‡ p < 0.01 versus PT, (B) Effect of 3 µM ESX on 1 nM Aldosterone (Ald)-stimulated Na-HCO 3 cotransporter (NBCe1) activity in isolated rat PTs (n = 6), (C) Effect of 3 µM esaxerenone (ESX) on 1 nM Ald-stimulated Na + -H + exchange transporter (NHE) activity in isolated rat PTs (n = 9). (D) Effects of small interfering RNA (siRNA) against Nr3c2 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6). (E) Effects of siRNA against Nr3c2 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (E) Effect of 10 µM GSK650394 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6), (G) Effect of 10 µM GSK650394 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (H) Effect of 10 µM PD98059 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6), (I) Effect of 10 µM PD98059 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (J) Ald-induced SGK1 and ERK phosphorylation and the effect of ESX on their phosphorylation in the rat renal cortex, (K) Ald-induced SGK1 and ERK phosphorylation and the effect of GSK650394 on their phosphorylation in the rat renal cortex, (L) Ald-induced SGK1 and ERK phosphorylation and the effect of PD98059 on their phosphorylation in the rat renal cortex. (B,C,F–I) *p < 0.05 versus untreated PTs; **p < 0.01 versus untreated PTs; † p < 0.05 versus ESX-treated PTs; ‡ p < 0.01 versus ESX-treated PTs; § p < 0.05 versus Ald and ESX-treated PTs; §§ p < 0.01 versus Ald and ESX-treated PTs. (D,E) **p < 0.01 versus control PTs treated with scrambled siRNA; ‡ p < 0.01 versus si-Nr3c2-treated PTs; §§ p < 0.01 versus Ald and si-Nr3c2-treated PTs.
Gene Exp Nr3c2 Rn00565562 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nr3c2/Gene+Exp%2E+Nr3c2%2C+Rn00565562_m1/pmc12738813-116-69-102
Average 86 stars, based on 1 article reviews
gene exp nr3c2 rn00565562 m1 - by Bioz Stars, 2026-09
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Proteintech anti mr
Effect of Ald on Na transport activity in proximal tubules (PTs) is mediated by the MR signaling pathway (A) <t>NR3C2</t> expression in the kidney and mRNA expression ratios in the glomeruli, proximal tubules (PTs), and other compartments isolated manually from fresh renal cortices. DT + CCD, distal tubule and cortical collecting duct. **p < 0.01 versus glomeruli; ‡ p < 0.01 versus PT, (B) Effect of 3 µM ESX on 1 nM Aldosterone (Ald)-stimulated Na-HCO 3 cotransporter (NBCe1) activity in isolated rat PTs (n = 6), (C) Effect of 3 µM esaxerenone (ESX) on 1 nM Ald-stimulated Na + -H + exchange transporter (NHE) activity in isolated rat PTs (n = 9). (D) Effects of small interfering RNA (siRNA) against Nr3c2 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6). (E) Effects of siRNA against Nr3c2 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (E) Effect of 10 µM GSK650394 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6), (G) Effect of 10 µM GSK650394 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (H) Effect of 10 µM PD98059 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6), (I) Effect of 10 µM PD98059 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (J) Ald-induced SGK1 and ERK phosphorylation and the effect of ESX on their phosphorylation in the rat renal cortex, (K) Ald-induced SGK1 and ERK phosphorylation and the effect of GSK650394 on their phosphorylation in the rat renal cortex, (L) Ald-induced SGK1 and ERK phosphorylation and the effect of PD98059 on their phosphorylation in the rat renal cortex. (B,C,F–I) *p < 0.05 versus untreated PTs; **p < 0.01 versus untreated PTs; † p < 0.05 versus ESX-treated PTs; ‡ p < 0.01 versus ESX-treated PTs; § p < 0.05 versus Ald and ESX-treated PTs; §§ p < 0.01 versus Ald and ESX-treated PTs. (D,E) **p < 0.01 versus control PTs treated with scrambled siRNA; ‡ p < 0.01 versus si-Nr3c2-treated PTs; §§ p < 0.01 versus Ald and si-Nr3c2-treated PTs.
Anti Mr, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nr3c2/NR3C2+Polyclonal+antibody/pm41579728-90-23-26
Average 93 stars, based on 1 article reviews
anti mr - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

91
Thermo Fisher gene exp nr3c2 mm01241596 m1
Effect of Ald on Na transport activity in proximal tubules (PTs) is mediated by the MR signaling pathway (A) <t>NR3C2</t> expression in the kidney and mRNA expression ratios in the glomeruli, proximal tubules (PTs), and other compartments isolated manually from fresh renal cortices. DT + CCD, distal tubule and cortical collecting duct. **p < 0.01 versus glomeruli; ‡ p < 0.01 versus PT, (B) Effect of 3 µM ESX on 1 nM Aldosterone (Ald)-stimulated Na-HCO 3 cotransporter (NBCe1) activity in isolated rat PTs (n = 6), (C) Effect of 3 µM esaxerenone (ESX) on 1 nM Ald-stimulated Na + -H + exchange transporter (NHE) activity in isolated rat PTs (n = 9). (D) Effects of small interfering RNA (siRNA) against Nr3c2 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6). (E) Effects of siRNA against Nr3c2 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (E) Effect of 10 µM GSK650394 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6), (G) Effect of 10 µM GSK650394 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (H) Effect of 10 µM PD98059 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6), (I) Effect of 10 µM PD98059 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (J) Ald-induced SGK1 and ERK phosphorylation and the effect of ESX on their phosphorylation in the rat renal cortex, (K) Ald-induced SGK1 and ERK phosphorylation and the effect of GSK650394 on their phosphorylation in the rat renal cortex, (L) Ald-induced SGK1 and ERK phosphorylation and the effect of PD98059 on their phosphorylation in the rat renal cortex. (B,C,F–I) *p < 0.05 versus untreated PTs; **p < 0.01 versus untreated PTs; † p < 0.05 versus ESX-treated PTs; ‡ p < 0.01 versus ESX-treated PTs; § p < 0.05 versus Ald and ESX-treated PTs; §§ p < 0.01 versus Ald and ESX-treated PTs. (D,E) **p < 0.01 versus control PTs treated with scrambled siRNA; ‡ p < 0.01 versus si-Nr3c2-treated PTs; §§ p < 0.01 versus Ald and si-Nr3c2-treated PTs.
Gene Exp Nr3c2 Mm01241596 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nr3c2/Gene+Exp%2E+nr3c2+mm01241596+m1/bio_rxiv__64898__2025__11__30__691444-50-66--1
Average 91 stars, based on 1 article reviews
gene exp nr3c2 mm01241596 m1 - by Bioz Stars, 2026-09
91/100 stars
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Proteintech nr3c2
Effect of Ald on Na transport activity in proximal tubules (PTs) is mediated by the MR signaling pathway (A) <t>NR3C2</t> expression in the kidney and mRNA expression ratios in the glomeruli, proximal tubules (PTs), and other compartments isolated manually from fresh renal cortices. DT + CCD, distal tubule and cortical collecting duct. **p < 0.01 versus glomeruli; ‡ p < 0.01 versus PT, (B) Effect of 3 µM ESX on 1 nM Aldosterone (Ald)-stimulated Na-HCO 3 cotransporter (NBCe1) activity in isolated rat PTs (n = 6), (C) Effect of 3 µM esaxerenone (ESX) on 1 nM Ald-stimulated Na + -H + exchange transporter (NHE) activity in isolated rat PTs (n = 9). (D) Effects of small interfering RNA (siRNA) against Nr3c2 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6). (E) Effects of siRNA against Nr3c2 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (E) Effect of 10 µM GSK650394 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6), (G) Effect of 10 µM GSK650394 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (H) Effect of 10 µM PD98059 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6), (I) Effect of 10 µM PD98059 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (J) Ald-induced SGK1 and ERK phosphorylation and the effect of ESX on their phosphorylation in the rat renal cortex, (K) Ald-induced SGK1 and ERK phosphorylation and the effect of GSK650394 on their phosphorylation in the rat renal cortex, (L) Ald-induced SGK1 and ERK phosphorylation and the effect of PD98059 on their phosphorylation in the rat renal cortex. (B,C,F–I) *p < 0.05 versus untreated PTs; **p < 0.01 versus untreated PTs; † p < 0.05 versus ESX-treated PTs; ‡ p < 0.01 versus ESX-treated PTs; § p < 0.05 versus Ald and ESX-treated PTs; §§ p < 0.01 versus Ald and ESX-treated PTs. (D,E) **p < 0.01 versus control PTs treated with scrambled siRNA; ‡ p < 0.01 versus si-Nr3c2-treated PTs; §§ p < 0.01 versus Ald and si-Nr3c2-treated PTs.
Nr3c2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nr3c2/NR3C2+Polyclonal+antibody/10__31083_slash_fbl45591-68-77-79
Average 93 stars, based on 1 article reviews
nr3c2 - by Bioz Stars, 2026-09
93/100 stars
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Proteintech antibodies against nr3c2
Effect of Ald on Na transport activity in proximal tubules (PTs) is mediated by the MR signaling pathway (A) <t>NR3C2</t> expression in the kidney and mRNA expression ratios in the glomeruli, proximal tubules (PTs), and other compartments isolated manually from fresh renal cortices. DT + CCD, distal tubule and cortical collecting duct. **p < 0.01 versus glomeruli; ‡ p < 0.01 versus PT, (B) Effect of 3 µM ESX on 1 nM Aldosterone (Ald)-stimulated Na-HCO 3 cotransporter (NBCe1) activity in isolated rat PTs (n = 6), (C) Effect of 3 µM esaxerenone (ESX) on 1 nM Ald-stimulated Na + -H + exchange transporter (NHE) activity in isolated rat PTs (n = 9). (D) Effects of small interfering RNA (siRNA) against Nr3c2 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6). (E) Effects of siRNA against Nr3c2 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (E) Effect of 10 µM GSK650394 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6), (G) Effect of 10 µM GSK650394 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (H) Effect of 10 µM PD98059 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6), (I) Effect of 10 µM PD98059 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (J) Ald-induced SGK1 and ERK phosphorylation and the effect of ESX on their phosphorylation in the rat renal cortex, (K) Ald-induced SGK1 and ERK phosphorylation and the effect of GSK650394 on their phosphorylation in the rat renal cortex, (L) Ald-induced SGK1 and ERK phosphorylation and the effect of PD98059 on their phosphorylation in the rat renal cortex. (B,C,F–I) *p < 0.05 versus untreated PTs; **p < 0.01 versus untreated PTs; † p < 0.05 versus ESX-treated PTs; ‡ p < 0.01 versus ESX-treated PTs; § p < 0.05 versus Ald and ESX-treated PTs; §§ p < 0.01 versus Ald and ESX-treated PTs. (D,E) **p < 0.01 versus control PTs treated with scrambled siRNA; ‡ p < 0.01 versus si-Nr3c2-treated PTs; §§ p < 0.01 versus Ald and si-Nr3c2-treated PTs.
Antibodies Against Nr3c2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nr3c2/NR3C2+Polyclonal+antibody/pm41177339-81-24-28
Average 93 stars, based on 1 article reviews
antibodies against nr3c2 - by Bioz Stars, 2026-09
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Image Search Results


Effect of Ald on Na transport activity in proximal tubules (PTs) is mediated by the MR signaling pathway (A) NR3C2 expression in the kidney and mRNA expression ratios in the glomeruli, proximal tubules (PTs), and other compartments isolated manually from fresh renal cortices. DT + CCD, distal tubule and cortical collecting duct. **p < 0.01 versus glomeruli; ‡ p < 0.01 versus PT, (B) Effect of 3 µM ESX on 1 nM Aldosterone (Ald)-stimulated Na-HCO 3 cotransporter (NBCe1) activity in isolated rat PTs (n = 6), (C) Effect of 3 µM esaxerenone (ESX) on 1 nM Ald-stimulated Na + -H + exchange transporter (NHE) activity in isolated rat PTs (n = 9). (D) Effects of small interfering RNA (siRNA) against Nr3c2 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6). (E) Effects of siRNA against Nr3c2 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (E) Effect of 10 µM GSK650394 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6), (G) Effect of 10 µM GSK650394 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (H) Effect of 10 µM PD98059 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6), (I) Effect of 10 µM PD98059 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (J) Ald-induced SGK1 and ERK phosphorylation and the effect of ESX on their phosphorylation in the rat renal cortex, (K) Ald-induced SGK1 and ERK phosphorylation and the effect of GSK650394 on their phosphorylation in the rat renal cortex, (L) Ald-induced SGK1 and ERK phosphorylation and the effect of PD98059 on their phosphorylation in the rat renal cortex. (B,C,F–I) *p < 0.05 versus untreated PTs; **p < 0.01 versus untreated PTs; † p < 0.05 versus ESX-treated PTs; ‡ p < 0.01 versus ESX-treated PTs; § p < 0.05 versus Ald and ESX-treated PTs; §§ p < 0.01 versus Ald and ESX-treated PTs. (D,E) **p < 0.01 versus control PTs treated with scrambled siRNA; ‡ p < 0.01 versus si-Nr3c2-treated PTs; §§ p < 0.01 versus Ald and si-Nr3c2-treated PTs.

Journal: Frontiers in Physiology

Article Title: Combined effect of esaxerenone and dapagliflozin on aldosterone-mediated sodium reabsorption and potassium excretion

doi: 10.3389/fphys.2025.1677518

Figure Lengend Snippet: Effect of Ald on Na transport activity in proximal tubules (PTs) is mediated by the MR signaling pathway (A) NR3C2 expression in the kidney and mRNA expression ratios in the glomeruli, proximal tubules (PTs), and other compartments isolated manually from fresh renal cortices. DT + CCD, distal tubule and cortical collecting duct. **p < 0.01 versus glomeruli; ‡ p < 0.01 versus PT, (B) Effect of 3 µM ESX on 1 nM Aldosterone (Ald)-stimulated Na-HCO 3 cotransporter (NBCe1) activity in isolated rat PTs (n = 6), (C) Effect of 3 µM esaxerenone (ESX) on 1 nM Ald-stimulated Na + -H + exchange transporter (NHE) activity in isolated rat PTs (n = 9). (D) Effects of small interfering RNA (siRNA) against Nr3c2 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6). (E) Effects of siRNA against Nr3c2 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (E) Effect of 10 µM GSK650394 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6), (G) Effect of 10 µM GSK650394 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (H) Effect of 10 µM PD98059 on 1 nM Ald-stimulated NBCe1 activity in isolated rat PTs (n = 6), (I) Effect of 10 µM PD98059 on 1 nM Ald-stimulated NHE activity in isolated rat PTs (n = 6), (J) Ald-induced SGK1 and ERK phosphorylation and the effect of ESX on their phosphorylation in the rat renal cortex, (K) Ald-induced SGK1 and ERK phosphorylation and the effect of GSK650394 on their phosphorylation in the rat renal cortex, (L) Ald-induced SGK1 and ERK phosphorylation and the effect of PD98059 on their phosphorylation in the rat renal cortex. (B,C,F–I) *p < 0.05 versus untreated PTs; **p < 0.01 versus untreated PTs; † p < 0.05 versus ESX-treated PTs; ‡ p < 0.01 versus ESX-treated PTs; § p < 0.05 versus Ald and ESX-treated PTs; §§ p < 0.01 versus Ald and ESX-treated PTs. (D,E) **p < 0.01 versus control PTs treated with scrambled siRNA; ‡ p < 0.01 versus si-Nr3c2-treated PTs; §§ p < 0.01 versus Ald and si-Nr3c2-treated PTs.

Article Snippet: Total RNA was extracted from tissues and cells using Isogen II (Nippon Gene, Tokyo, Japan) per the manufacturer’s instructions and first-strand cDNA was synthesized using a cDNA Synthesis Kit (Takara, Tokyo, Japan) as previously reported ( ; ; ). mRNA expression levels were estimated using quantitative PCR (Prism 7000; Applied Biosystems, Foster City, CA, USA) with TaqMan Gene Expression Master Mix (Applied Biosystems) and TaqMan Gene Expression Assay kits (Rn00565562_m1 for rat Nr3c2, Rn00582881_m1 for rat Kcnma1, Rn00583376_m1 for rat Kcnq1 , Rn01641410_m1 for rat Kcnk1 , Rn01755927_m1 for rat Kcnk5 , Rn02377069_s1 for rat Kcne3 , and Rn00667869_m1 for rat β-actin; Thermo Fisher Scientific, Waltham, MA, USA). mRNA levels were normalized to β-actin expression levels.

Techniques: Activity Assay, Expressing, Isolation, Small Interfering RNA, Phospho-proteomics, Control