anti nr2a  (Proteintech)

Journal: PLOS One

Article Title: The role of DAAO in cognitive impairment of offspring mice induced by arsenic exposure during early developmental stage

doi: 10.1371/journal.pone.0333414

Figure Lengend Snippet: (A, C, E) Real-time RT-PCR was applied to detect NR1, NR2A and NR2B mRNA levels. (B, D, F) Protein levels of NR1, NR2A and NR2B were measured by WB. Images were the representative blots and statistical analysis of protein levels. Data were shown as mean ± SD, n = 6. P < 0.05, * vs. the control, # vs. NaAsO 2 group.

Article Snippet: Antibodies were DAAO (1:500), NR1 (1:500) (Boster, China), SR (1:500), NR2A (1:500), NR2B (1:500) (Proteintech, China), SYP (1:1000), PSD95 (1:1000) (Abcam, UK), β-actin (1:10000) (Santa Cruz, USA).

Techniques: Quantitative RT-PCR, Control

Journal: Translational Psychiatry

Article Title: Chronic postoperative pain induces contextual fear extinction dysfunction through hippocampal NMDAR/BDNF/TrkB signaling pathway in mice

doi: 10.1038/s41398-025-03417-0

Figure Lengend Snippet: A Representative Western blot analysis of NR2B、NR2A、P-ERK、ERK、P-CREB and CREB in hippocampus. β-Tublin was included as loading control. B Quantitative analysis of NR2B levels (n = 4). C Quantitative analysis of NR2A levels (n = 4). D Quantitative analysis of P-Erk1/2 levels (n = 4). E Quantitative analysis of P-CREB levels (n = 4). F Representative Western blot analysis of PSD-95 in hippocampus. β-Tublin was included as loading Control. G Quantitative analysis of PSD-95 levels (n = 4). H Experimental flow chart. I Representative map of dendritic spine density of neurons in hippocampus. J Quantitative statistics of dendritic spine density (n = 6). Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01.

Article Snippet: The membrane was blocked using TBST containing 0.1% Tween-20 and 5% skim milk, followed by incubation with the following antibodies: anti-NR2A(1:1000, #4205S, Cell Signaling Technology, USA); anti-NR2B(1:1000, #14544S, Cell Signaling Technology, USA); anti-ERK1/2(1:1000, #4695 T, Cell Signaling Technology, USA); anti-P-p44/42MAPK(1:1000, #9101S, Cell Signaling Technology, USA); anti-CREB(1:1000, #9197 T, Cell Signaling Technology, USA); anti-P-CREB(1:1000, #9198S, Cell Signaling Technology, USA); anti-BDNF(1:1000, #EPR1292, Abcam, USA); anti-β3-Tubllin(1:1000, #5666S, Cell Signaling Technology, USA); anti-PSD-95(1:1000, #2507S, Cell Signaling Technology, USA); anti-TrkB(1:1000, #4603, Cell Signaling Technology, USA); anti-TrkB(B-3) (1:500, #sc-7268, Santa Cruz Biotechnology, USA).

Techniques: Western Blot, Control

Journal: Frontiers in Behavioral Neuroscience

Article Title: Effects of propofol on the cognition and hippocampal N-methyl-D-aspartate subunits expression in an MPTP-induced Parkinson’s disease rat model

doi: 10.3389/fnbeh.2025.1607421

Figure Lengend Snippet: Western blot for detecting the distribution of hippocampal NMDA receptor NR2A and NR2B subunits in the acute (A) and chronic (B) intervention groups. Statistical analysis revealed that MPTP-lesioning caused an imbalance of NR2A/NR2B ratio when compared with the sham groups. And, chronic treatment with propofol increases the imbalance of NR2A/NR2B ratio in the hippocampus in the MPTP-induced PD rat model. * p < 0.05 and ** p < 0.001 compared with the control (sham) group. # p < 0.05 and ## p < 0.001 compared with the MPTP group, n = 5 in each group.

Article Snippet: Membranes were blocked in 5% BSA/TBST for 1 h at room temperature and then incubated overnight with NR2A (1:500, AB1555P, Millipore, Billerica, MA) or NR2B (1:500, AB1557P, Millipore, Billerica, MA) in 5% BSA/TBST at 4°C.

Techniques: Western Blot, Control

Journal: Frontiers in Behavioral Neuroscience

Article Title: Effects of propofol on the cognition and hippocampal N-methyl-D-aspartate subunits expression in an MPTP-induced Parkinson’s disease rat model

doi: 10.3389/fnbeh.2025.1607421

Figure Lengend Snippet: The expression levels of hippocampal NMDA receptor NR2A and NR2B mRNA in the acute (A) and chronic (B) intervention groups were quantified by RT-qPCR. In the chronic intervention groups, propofol increases the NR2A/NR2B mRNA ratio in the hippocampus in the MPTP-induced PD rat model. * p < 0.05 and ** p < 0.001 compared with the control (sham) group. # p < 0.05 and ## p < 0.001 compared with the MPTP group, n = 5 in each group.

Article Snippet: Membranes were blocked in 5% BSA/TBST for 1 h at room temperature and then incubated overnight with NR2A (1:500, AB1555P, Millipore, Billerica, MA) or NR2B (1:500, AB1557P, Millipore, Billerica, MA) in 5% BSA/TBST at 4°C.

Techniques: Expressing, Quantitative RT-PCR, Control