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npgbkt7  (TaKaRa)


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    Structured Review

    TaKaRa npgbkt7
    Plasmids carrying on the fetal brain cDNA library were co-transformed into the <t>NpGBKT7-</t> SelR′ -containing yeast and screened by the selection plate for the blue colonies (A). The interaction between SelR′ and Clu was verified by re-transformation of the plasmids NpGBKT7- SelR′ and pACT2- Clu into either AH109 (B) or Y2HGold (C) yeast cells. Yeast cells in B (1–3) were transformed with single NpGBKT7- SelR′ , NpGBKT7- SelR′ plus pACT2, and NpGBKT7- SelR′ plus pACT2- Clu plasmids, respectively. Yeast cells in C, D, E were transformed with NpGBKT7- SelR′ plus pACT2- Clu , pGBKT7- p53 plus pADT7- T (positive control), and pGBKT7- Lam plus pADT7- T (negative control), respectively, followed by the selection on SD/−Leu/−Trp/X-α-Gal/Aba plates.
    Npgbkt7, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/npgbkt7/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    npgbkt7 - by Bioz Stars, 2026-06
    86/100 stars

    Images

    1) Product Images from "Direct Interaction of Selenoprotein R with Clusterin and Its Possible Role in Alzheimer’s Disease"

    Article Title: Direct Interaction of Selenoprotein R with Clusterin and Its Possible Role in Alzheimer’s Disease

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0066384

    Plasmids carrying on the fetal brain cDNA library were co-transformed into the NpGBKT7- SelR′ -containing yeast and screened by the selection plate for the blue colonies (A). The interaction between SelR′ and Clu was verified by re-transformation of the plasmids NpGBKT7- SelR′ and pACT2- Clu into either AH109 (B) or Y2HGold (C) yeast cells. Yeast cells in B (1–3) were transformed with single NpGBKT7- SelR′ , NpGBKT7- SelR′ plus pACT2, and NpGBKT7- SelR′ plus pACT2- Clu plasmids, respectively. Yeast cells in C, D, E were transformed with NpGBKT7- SelR′ plus pACT2- Clu , pGBKT7- p53 plus pADT7- T (positive control), and pGBKT7- Lam plus pADT7- T (negative control), respectively, followed by the selection on SD/−Leu/−Trp/X-α-Gal/Aba plates.
    Figure Legend Snippet: Plasmids carrying on the fetal brain cDNA library were co-transformed into the NpGBKT7- SelR′ -containing yeast and screened by the selection plate for the blue colonies (A). The interaction between SelR′ and Clu was verified by re-transformation of the plasmids NpGBKT7- SelR′ and pACT2- Clu into either AH109 (B) or Y2HGold (C) yeast cells. Yeast cells in B (1–3) were transformed with single NpGBKT7- SelR′ , NpGBKT7- SelR′ plus pACT2, and NpGBKT7- SelR′ plus pACT2- Clu plasmids, respectively. Yeast cells in C, D, E were transformed with NpGBKT7- SelR′ plus pACT2- Clu , pGBKT7- p53 plus pADT7- T (positive control), and pGBKT7- Lam plus pADT7- T (negative control), respectively, followed by the selection on SD/−Leu/−Trp/X-α-Gal/Aba plates.

    Techniques Used: cDNA Library Assay, Transformation Assay, Selection, Positive Control, Negative Control



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    npgbkt7  (TaKaRa)
    86
    TaKaRa npgbkt7
    Plasmids carrying on the fetal brain cDNA library were co-transformed into the <t>NpGBKT7-</t> SelR′ -containing yeast and screened by the selection plate for the blue colonies (A). The interaction between SelR′ and Clu was verified by re-transformation of the plasmids NpGBKT7- SelR′ and pACT2- Clu into either AH109 (B) or Y2HGold (C) yeast cells. Yeast cells in B (1–3) were transformed with single NpGBKT7- SelR′ , NpGBKT7- SelR′ plus pACT2, and NpGBKT7- SelR′ plus pACT2- Clu plasmids, respectively. Yeast cells in C, D, E were transformed with NpGBKT7- SelR′ plus pACT2- Clu , pGBKT7- p53 plus pADT7- T (positive control), and pGBKT7- Lam plus pADT7- T (negative control), respectively, followed by the selection on SD/−Leu/−Trp/X-α-Gal/Aba plates.
    Npgbkt7, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/npgbkt7/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    npgbkt7 - by Bioz Stars, 2026-06
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Plasmids carrying on the fetal brain cDNA library were co-transformed into the NpGBKT7- SelR′ -containing yeast and screened by the selection plate for the blue colonies (A). The interaction between SelR′ and Clu was verified by re-transformation of the plasmids NpGBKT7- SelR′ and pACT2- Clu into either AH109 (B) or Y2HGold (C) yeast cells. Yeast cells in B (1–3) were transformed with single NpGBKT7- SelR′ , NpGBKT7- SelR′ plus pACT2, and NpGBKT7- SelR′ plus pACT2- Clu plasmids, respectively. Yeast cells in C, D, E were transformed with NpGBKT7- SelR′ plus pACT2- Clu , pGBKT7- p53 plus pADT7- T (positive control), and pGBKT7- Lam plus pADT7- T (negative control), respectively, followed by the selection on SD/−Leu/−Trp/X-α-Gal/Aba plates.

    Journal: PLoS ONE

    Article Title: Direct Interaction of Selenoprotein R with Clusterin and Its Possible Role in Alzheimer’s Disease

    doi: 10.1371/journal.pone.0066384

    Figure Lengend Snippet: Plasmids carrying on the fetal brain cDNA library were co-transformed into the NpGBKT7- SelR′ -containing yeast and screened by the selection plate for the blue colonies (A). The interaction between SelR′ and Clu was verified by re-transformation of the plasmids NpGBKT7- SelR′ and pACT2- Clu into either AH109 (B) or Y2HGold (C) yeast cells. Yeast cells in B (1–3) were transformed with single NpGBKT7- SelR′ , NpGBKT7- SelR′ plus pACT2, and NpGBKT7- SelR′ plus pACT2- Clu plasmids, respectively. Yeast cells in C, D, E were transformed with NpGBKT7- SelR′ plus pACT2- Clu , pGBKT7- p53 plus pADT7- T (positive control), and pGBKT7- Lam plus pADT7- T (negative control), respectively, followed by the selection on SD/−Leu/−Trp/X-α-Gal/Aba plates.

    Article Snippet: Matchmaker™ Gold yeast two-hybrid system, yeast strains Y2HGold and AH109, plasmids pACT2 and NpGBKT7, and human fetal brain cDNA library (using pACT2 as the vector) were purchased from Clontech Laboratories (Mountainview, CA, USA).

    Techniques: cDNA Library Assay, Transformation Assay, Selection, Positive Control, Negative Control