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p aeruginosa atcc  (ATCC)


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    Structured Review

    ATCC p aeruginosa atcc
    Survival of different bacterial strains upon disinfection with steam. The different plots present the colony-forming units (CFUs) of various bacterial strains before and after steam treatment for 10, 30–60 s and subsequent plating on LB agar: ( a ). S. aureus ATCC15981 (MSSA); ( b ). S. aureus E75 (MSSA); ( c ). S. aureus E166 (MSSA); ( d ). S. aureus E276 (MSSA); ( e ). S. aureus USA300 (MRSA); ( f ). S. aureus HG001 (MSSA); ( g ). S. epidermidis ATCC35984; h. E. coli ATCC25922; i. P. <t>aeruginosa</t> ATCC27853; j. K. oxytoca ; k. K. pneumoniae ATCC15883; and l. A. baumannii ATCC19606. The residual CFU counts are indicated as a percentage of the CFU counts of the inoculum on top of each bar. All experiments were performed in triplicate ( n = 3). Standard deviations in the CFU counts are indicated by error bars. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
    P Aeruginosa Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Disinfection of medical devices with a steam machine that operates at atmospheric pressure and is suitable for home usage"

    Article Title: Disinfection of medical devices with a steam machine that operates at atmospheric pressure and is suitable for home usage

    Journal: Scientific Reports

    doi: 10.1038/s41598-025-11509-6

    Survival of different bacterial strains upon disinfection with steam. The different plots present the colony-forming units (CFUs) of various bacterial strains before and after steam treatment for 10, 30–60 s and subsequent plating on LB agar: ( a ). S. aureus ATCC15981 (MSSA); ( b ). S. aureus E75 (MSSA); ( c ). S. aureus E166 (MSSA); ( d ). S. aureus E276 (MSSA); ( e ). S. aureus USA300 (MRSA); ( f ). S. aureus HG001 (MSSA); ( g ). S. epidermidis ATCC35984; h. E. coli ATCC25922; i. P. aeruginosa ATCC27853; j. K. oxytoca ; k. K. pneumoniae ATCC15883; and l. A. baumannii ATCC19606. The residual CFU counts are indicated as a percentage of the CFU counts of the inoculum on top of each bar. All experiments were performed in triplicate ( n = 3). Standard deviations in the CFU counts are indicated by error bars. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
    Figure Legend Snippet: Survival of different bacterial strains upon disinfection with steam. The different plots present the colony-forming units (CFUs) of various bacterial strains before and after steam treatment for 10, 30–60 s and subsequent plating on LB agar: ( a ). S. aureus ATCC15981 (MSSA); ( b ). S. aureus E75 (MSSA); ( c ). S. aureus E166 (MSSA); ( d ). S. aureus E276 (MSSA); ( e ). S. aureus USA300 (MRSA); ( f ). S. aureus HG001 (MSSA); ( g ). S. epidermidis ATCC35984; h. E. coli ATCC25922; i. P. aeruginosa ATCC27853; j. K. oxytoca ; k. K. pneumoniae ATCC15883; and l. A. baumannii ATCC19606. The residual CFU counts are indicated as a percentage of the CFU counts of the inoculum on top of each bar. All experiments were performed in triplicate ( n = 3). Standard deviations in the CFU counts are indicated by error bars. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

    Techniques Used: Serial Time-encoded Amplified Microscopy

    Disinfection of nebulizer parts with steam. The different parts of a PARI LC SPRINT Nebulizer handset were contaminated with: ( a ) S. aureus ATCC15981; ( b ) S. aureus E75; (c) S. aureus E166; ( d ) S. aureus E276; ( e ) S. aureus USA300; ( f ) S. aureus HG001; ( g ) S. epidermidis ATCC35984; ( h ) E. coli ATCC25922; ( i ) P. aeruginosa ATCC27853; ( j ) K. oxytoca ; ( k ) K. pneumoniae ATCC15883; ( l ) A. baumanni ATCC19606. Prior and after 60 s steam exposure, the contaminated mouth piece was replica-plated on LB agar. Bacteria contaminating the connection tube and nozzle were collected by swabbing and subsequently plated on LB agar. Of note, the different bacterial loads on the contaminated nebulizer parts were not quantified. Upper left plate of each panel, replica-plated contaminated mouthpiece; lower left plate, replica-plated contaminated mouthpiece after disinfection with steam; upper right part plate, bacteria contaminating the connection tube and nozzle; lower right plate, bacteria contaminating the connection tube and nozzle after steam disinfection. Three independent replicate experiments were performed ( n = 3).
    Figure Legend Snippet: Disinfection of nebulizer parts with steam. The different parts of a PARI LC SPRINT Nebulizer handset were contaminated with: ( a ) S. aureus ATCC15981; ( b ) S. aureus E75; (c) S. aureus E166; ( d ) S. aureus E276; ( e ) S. aureus USA300; ( f ) S. aureus HG001; ( g ) S. epidermidis ATCC35984; ( h ) E. coli ATCC25922; ( i ) P. aeruginosa ATCC27853; ( j ) K. oxytoca ; ( k ) K. pneumoniae ATCC15883; ( l ) A. baumanni ATCC19606. Prior and after 60 s steam exposure, the contaminated mouth piece was replica-plated on LB agar. Bacteria contaminating the connection tube and nozzle were collected by swabbing and subsequently plated on LB agar. Of note, the different bacterial loads on the contaminated nebulizer parts were not quantified. Upper left plate of each panel, replica-plated contaminated mouthpiece; lower left plate, replica-plated contaminated mouthpiece after disinfection with steam; upper right part plate, bacteria contaminating the connection tube and nozzle; lower right plate, bacteria contaminating the connection tube and nozzle after steam disinfection. Three independent replicate experiments were performed ( n = 3).

    Techniques Used: Serial Time-encoded Amplified Microscopy, Bacteria

    Effects of steam exposure on bacterial biofilms. Biofilms of different bacterial species were allowed to form in 96-well micro liter plates during 24 h of growth. Subsequently, the biofilms were exposed to steam for 1, 5–10 min. The biofilms and a non-sterilized control plate were then stained with crystal violet ( a ). In addition, the crystal violet retained by the biofilms was dissolved in ethanol and the released crystal violet was quantified spectrophotometrically ( b ). The bacterial strains used were S. aureus ATCC15981, S. aureus E75, S. aureus E166, S. aureus E276, S. aureus USA300, S. aureus HG001, S. epidermidis ATCC35984, E. coli ATCC25922, P. aeruginosa ATCC27853, K. oxytoca , K. pneumoniae ATCC15883 and A. baumannii ATCC19606. Experiments were performed in triplicate ( n = 3). Standard deviations in the CFU counts are indicated by error bars. *, P < 0.05.
    Figure Legend Snippet: Effects of steam exposure on bacterial biofilms. Biofilms of different bacterial species were allowed to form in 96-well micro liter plates during 24 h of growth. Subsequently, the biofilms were exposed to steam for 1, 5–10 min. The biofilms and a non-sterilized control plate were then stained with crystal violet ( a ). In addition, the crystal violet retained by the biofilms was dissolved in ethanol and the released crystal violet was quantified spectrophotometrically ( b ). The bacterial strains used were S. aureus ATCC15981, S. aureus E75, S. aureus E166, S. aureus E276, S. aureus USA300, S. aureus HG001, S. epidermidis ATCC35984, E. coli ATCC25922, P. aeruginosa ATCC27853, K. oxytoca , K. pneumoniae ATCC15883 and A. baumannii ATCC19606. Experiments were performed in triplicate ( n = 3). Standard deviations in the CFU counts are indicated by error bars. *, P < 0.05.

    Techniques Used: Serial Time-encoded Amplified Microscopy, Control, Staining

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    Survival of different bacterial strains upon disinfection with steam. The different plots present the colony-forming units (CFUs) of various bacterial strains before and after steam treatment for 10, 30–60 s and subsequent plating on LB agar: ( a ). S. aureus ATCC15981 (MSSA); ( b ). S. aureus E75 (MSSA); ( c ). S. aureus E166 (MSSA); ( d ). S. aureus E276 (MSSA); ( e ). S. aureus USA300 (MRSA); ( f ). S. aureus HG001 (MSSA); ( g ). S. epidermidis ATCC35984; h. E. coli ATCC25922; i. P. <t>aeruginosa</t> ATCC27853; j. K. oxytoca ; k. K. pneumoniae ATCC15883; and l. A. baumannii ATCC19606. The residual CFU counts are indicated as a percentage of the CFU counts of the inoculum on top of each bar. All experiments were performed in triplicate ( n = 3). Standard deviations in the CFU counts are indicated by error bars. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
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    ATCC nonomuraea roseoviolacea
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    Survival of different bacterial strains upon disinfection with steam. The different plots present the colony-forming units (CFUs) of various bacterial strains before and after steam treatment for 10, 30–60 s and subsequent plating on LB agar: ( a ). S. aureus ATCC15981 (MSSA); ( b ). S. aureus E75 (MSSA); ( c ). S. aureus E166 (MSSA); ( d ). S. aureus E276 (MSSA); ( e ). S. aureus USA300 (MRSA); ( f ). S. aureus HG001 (MSSA); ( g ). S. epidermidis ATCC35984; h. E. coli ATCC25922; i. P. aeruginosa ATCC27853; j. K. oxytoca ; k. K. pneumoniae ATCC15883; and l. A. baumannii ATCC19606. The residual CFU counts are indicated as a percentage of the CFU counts of the inoculum on top of each bar. All experiments were performed in triplicate ( n = 3). Standard deviations in the CFU counts are indicated by error bars. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

    Journal: Scientific Reports

    Article Title: Disinfection of medical devices with a steam machine that operates at atmospheric pressure and is suitable for home usage

    doi: 10.1038/s41598-025-11509-6

    Figure Lengend Snippet: Survival of different bacterial strains upon disinfection with steam. The different plots present the colony-forming units (CFUs) of various bacterial strains before and after steam treatment for 10, 30–60 s and subsequent plating on LB agar: ( a ). S. aureus ATCC15981 (MSSA); ( b ). S. aureus E75 (MSSA); ( c ). S. aureus E166 (MSSA); ( d ). S. aureus E276 (MSSA); ( e ). S. aureus USA300 (MRSA); ( f ). S. aureus HG001 (MSSA); ( g ). S. epidermidis ATCC35984; h. E. coli ATCC25922; i. P. aeruginosa ATCC27853; j. K. oxytoca ; k. K. pneumoniae ATCC15883; and l. A. baumannii ATCC19606. The residual CFU counts are indicated as a percentage of the CFU counts of the inoculum on top of each bar. All experiments were performed in triplicate ( n = 3). Standard deviations in the CFU counts are indicated by error bars. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

    Article Snippet: P. aeruginosa ATCC , 2.8 ± 0.58*10 8 , 13.3 ± 2.7*10 3 , 0 , 0.

    Techniques: Serial Time-encoded Amplified Microscopy

    Disinfection of nebulizer parts with steam. The different parts of a PARI LC SPRINT Nebulizer handset were contaminated with: ( a ) S. aureus ATCC15981; ( b ) S. aureus E75; (c) S. aureus E166; ( d ) S. aureus E276; ( e ) S. aureus USA300; ( f ) S. aureus HG001; ( g ) S. epidermidis ATCC35984; ( h ) E. coli ATCC25922; ( i ) P. aeruginosa ATCC27853; ( j ) K. oxytoca ; ( k ) K. pneumoniae ATCC15883; ( l ) A. baumanni ATCC19606. Prior and after 60 s steam exposure, the contaminated mouth piece was replica-plated on LB agar. Bacteria contaminating the connection tube and nozzle were collected by swabbing and subsequently plated on LB agar. Of note, the different bacterial loads on the contaminated nebulizer parts were not quantified. Upper left plate of each panel, replica-plated contaminated mouthpiece; lower left plate, replica-plated contaminated mouthpiece after disinfection with steam; upper right part plate, bacteria contaminating the connection tube and nozzle; lower right plate, bacteria contaminating the connection tube and nozzle after steam disinfection. Three independent replicate experiments were performed ( n = 3).

    Journal: Scientific Reports

    Article Title: Disinfection of medical devices with a steam machine that operates at atmospheric pressure and is suitable for home usage

    doi: 10.1038/s41598-025-11509-6

    Figure Lengend Snippet: Disinfection of nebulizer parts with steam. The different parts of a PARI LC SPRINT Nebulizer handset were contaminated with: ( a ) S. aureus ATCC15981; ( b ) S. aureus E75; (c) S. aureus E166; ( d ) S. aureus E276; ( e ) S. aureus USA300; ( f ) S. aureus HG001; ( g ) S. epidermidis ATCC35984; ( h ) E. coli ATCC25922; ( i ) P. aeruginosa ATCC27853; ( j ) K. oxytoca ; ( k ) K. pneumoniae ATCC15883; ( l ) A. baumanni ATCC19606. Prior and after 60 s steam exposure, the contaminated mouth piece was replica-plated on LB agar. Bacteria contaminating the connection tube and nozzle were collected by swabbing and subsequently plated on LB agar. Of note, the different bacterial loads on the contaminated nebulizer parts were not quantified. Upper left plate of each panel, replica-plated contaminated mouthpiece; lower left plate, replica-plated contaminated mouthpiece after disinfection with steam; upper right part plate, bacteria contaminating the connection tube and nozzle; lower right plate, bacteria contaminating the connection tube and nozzle after steam disinfection. Three independent replicate experiments were performed ( n = 3).

    Article Snippet: P. aeruginosa ATCC , 2.8 ± 0.58*10 8 , 13.3 ± 2.7*10 3 , 0 , 0.

    Techniques: Serial Time-encoded Amplified Microscopy, Bacteria

    Effects of steam exposure on bacterial biofilms. Biofilms of different bacterial species were allowed to form in 96-well micro liter plates during 24 h of growth. Subsequently, the biofilms were exposed to steam for 1, 5–10 min. The biofilms and a non-sterilized control plate were then stained with crystal violet ( a ). In addition, the crystal violet retained by the biofilms was dissolved in ethanol and the released crystal violet was quantified spectrophotometrically ( b ). The bacterial strains used were S. aureus ATCC15981, S. aureus E75, S. aureus E166, S. aureus E276, S. aureus USA300, S. aureus HG001, S. epidermidis ATCC35984, E. coli ATCC25922, P. aeruginosa ATCC27853, K. oxytoca , K. pneumoniae ATCC15883 and A. baumannii ATCC19606. Experiments were performed in triplicate ( n = 3). Standard deviations in the CFU counts are indicated by error bars. *, P < 0.05.

    Journal: Scientific Reports

    Article Title: Disinfection of medical devices with a steam machine that operates at atmospheric pressure and is suitable for home usage

    doi: 10.1038/s41598-025-11509-6

    Figure Lengend Snippet: Effects of steam exposure on bacterial biofilms. Biofilms of different bacterial species were allowed to form in 96-well micro liter plates during 24 h of growth. Subsequently, the biofilms were exposed to steam for 1, 5–10 min. The biofilms and a non-sterilized control plate were then stained with crystal violet ( a ). In addition, the crystal violet retained by the biofilms was dissolved in ethanol and the released crystal violet was quantified spectrophotometrically ( b ). The bacterial strains used were S. aureus ATCC15981, S. aureus E75, S. aureus E166, S. aureus E276, S. aureus USA300, S. aureus HG001, S. epidermidis ATCC35984, E. coli ATCC25922, P. aeruginosa ATCC27853, K. oxytoca , K. pneumoniae ATCC15883 and A. baumannii ATCC19606. Experiments were performed in triplicate ( n = 3). Standard deviations in the CFU counts are indicated by error bars. *, P < 0.05.

    Article Snippet: P. aeruginosa ATCC , 2.8 ± 0.58*10 8 , 13.3 ± 2.7*10 3 , 0 , 0.

    Techniques: Serial Time-encoded Amplified Microscopy, Control, Staining

    Maximum-likelihood core genes phylogenomic tree of proposed taxonomic names of Nonomuraea species and subspecies constructed using the Roary pangenome pipeline and MEGA software version 11 with 1000 bootstrap replications to assess statistical support. This illustrates the evolutionary relationship between the species of Nonomuraea . Scientific names shown in parenthesis correspond to the current scientific names. Names in bold are to indicate the proposed taxonomic changes. Saccharothrix algeriensis DSM 44581 T was used as an outgroup. The scale bar represents 0.02 nucleotide substitutions per sequence position (site). Accession numbers of the genome sequences used for the reconstruction are shown in .

    Journal: PLOS One

    Article Title: Whole genome-based reclassification of several species of the genus Nonomuraea

    doi: 10.1371/journal.pone.0327003

    Figure Lengend Snippet: Maximum-likelihood core genes phylogenomic tree of proposed taxonomic names of Nonomuraea species and subspecies constructed using the Roary pangenome pipeline and MEGA software version 11 with 1000 bootstrap replications to assess statistical support. This illustrates the evolutionary relationship between the species of Nonomuraea . Scientific names shown in parenthesis correspond to the current scientific names. Names in bold are to indicate the proposed taxonomic changes. Saccharothrix algeriensis DSM 44581 T was used as an outgroup. The scale bar represents 0.02 nucleotide substitutions per sequence position (site). Accession numbers of the genome sequences used for the reconstruction are shown in .

    Article Snippet: Based on comprehensive genomic studies, our results support the elevation of both Nonomuraea roseoviolacea subsp. carminata and Nonomuraea roseoviolacea subsp. roseoviolacea to an unified species without subspecies distinction: Nonomuraea roseoviolacea (ATCC 27297 T = BCRC 13406 T = CBS 260.72 T = CCM 3491 T = CCRC 13406 T = CGMCC 4.1072 T = CIP 106924 T = DSM 43144 T = IFO 14098 T = IMET 9751 T = JCM 3145 T = KCTC 9283 T = NBRC 14098 T = NCIB 11117 T = NCIMB 11117 T = NRRL B-16127 T = VKM Ac-909 T ).

    Techniques: Construct, Software, Sequencing

    Maximum-likelihood core genes phylogenomic tree of proposed taxonomic names of Nonomuraea species and subspecies constructed using the Roary pangenome pipeline and MEGA software version 11 with 1000 bootstrap replications to assess statistical support. This illustrates the evolutionary relationship between the species of Nonomuraea . Scientific names shown in parenthesis correspond to the current scientific names. Names in bold are to indicate the proposed taxonomic changes. Saccharothrix algeriensis DSM 44581 T was used as an outgroup. The scale bar represents 0.02 nucleotide substitutions per sequence position (site). Accession numbers of the genome sequences used for the reconstruction are shown in .

    Journal: PLOS One

    Article Title: Whole genome-based reclassification of several species of the genus Nonomuraea

    doi: 10.1371/journal.pone.0327003

    Figure Lengend Snippet: Maximum-likelihood core genes phylogenomic tree of proposed taxonomic names of Nonomuraea species and subspecies constructed using the Roary pangenome pipeline and MEGA software version 11 with 1000 bootstrap replications to assess statistical support. This illustrates the evolutionary relationship between the species of Nonomuraea . Scientific names shown in parenthesis correspond to the current scientific names. Names in bold are to indicate the proposed taxonomic changes. Saccharothrix algeriensis DSM 44581 T was used as an outgroup. The scale bar represents 0.02 nucleotide substitutions per sequence position (site). Accession numbers of the genome sequences used for the reconstruction are shown in .

    Article Snippet: Based on comprehensive genomic studies, our results support the elevation of both Nonomuraea roseoviolacea subsp. carminata and Nonomuraea roseoviolacea subsp. roseoviolacea to an unified species without subspecies distinction: Nonomuraea roseoviolacea (ATCC 27297 T = BCRC 13406 T = CBS 260.72 T = CCM 3491 T = CCRC 13406 T = CGMCC 4.1072 T = CIP 106924 T = DSM 43144 T = IFO 14098 T = IMET 9751 T = JCM 3145 T = KCTC 9283 T = NBRC 14098 T = NCIB 11117 T = NCIMB 11117 T = NRRL B-16127 T = VKM Ac-909 T ).

    Techniques: Construct, Software, Sequencing