nocodazole  (MedChemExpress)

Journal: Signal Transduction and Targeted Therapy

Article Title: Interaction between CDC6 and Tmod3 accelerates resistance to paclitaxel through focal adhesion assembly

doi: 10.1038/s41392-025-02490-7

Figure Lengend Snippet: Tmod3 and CDC6 accelerate focal adhesion assembly. a Paxillin and actin filaments in wild type cells and CDC6-KO cells (n = 10 images; ****P < 0.0001). Scale bar, 10 µm. b Changes in the protein expression of CDC6, paxillin and p-paxillin in A549/PTX wild-type and CDC6-KO cells (n = 3; ****P < 0.0001). c Changes in the protein expression of CDC6, paxillin and p-paxillin after treatment with different concentrations of Blebb and CytoD for 72 h (n = 3; ****P < 0.0001, ## P < 0.01, #### P < 0.0001). d , e Paxillin in Tmod3- and Tmod1-depleted cells was visualized via confocal microscopy (n = 10 images; ****P < 0.0001). Scale bar, 10 µm. f , g Wild-type and CDC6-KO cells were exposed to CytoD (5 μM) for 3 h. The cells were fixed at the indicated time points after CytoD washout, stained with phalloidin and paxillin, and visualized via confocal microscopy. Scar bar, 10 μm. h , i Immunofluorescence analysis of paxillin in HeLa/CDC6 EGFP cells after nocodazole (10 μM) treatment for 4 h which were then washed out for the indicated times. Scale bar, 10 μm. Wild-type cells (dotted line) and CDC6 EGFP -overexpression cells (solid line) are magnified. j A549/PTX cells were exposed to paclitaxel, defactinib or a combination of paclitaxel and defactinib for 72 h. Cell viability was determined via the MTT assay (n = 3; ***P < 0.001). The data are presented as the means ± SDs

Article Snippet: The confocal dishes were seeded with 1 × 10 5 HeLa cells that were transiently transfected with the CDC6-EGFP plasmid and cultured for 48 h. The cells were exposed to 10 μM nocodazole (TargetMol, T2802) for 4 h to depolymerize the microtubes completely.

Techniques: Expressing, Confocal Microscopy, Staining, Immunofluorescence, Over Expression, MTT Assay