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nmda  (Tocris)


Bioz Verified Symbol Tocris is a verified supplier
Bioz Manufacturer Symbol Tocris manufactures this product  
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    Tocris nmda
    Nmda, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 2093 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nmda/NMDA/pm41966427-137-20-21
    Average 96 stars, based on 2093 article reviews
    nmda - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Membrane:

    Article Title: GluN2A-mediated currents and calcium signal in human iPSC-derived neurons
    Article Snippet: .. Membrane currents elicited by NMDA (TOCRIS, HB0454) and Glycine (Millipore Sigma, 106-57-0) were recorded at a membrane potential of -60 mV using the whole-cell configuration of the patch-clamp technique with a List Electronics EPC-7 amplifier. ..

    Article Title: GluN2A-mediated currents and calcium signal in human iPSC-derived neurons.
    Article Snippet: .. Membrane currents elicited by NMDA (TOCRIS, HB0454) and Glycine (Millipore Sigma, 106-57-0) were recorded at a membrane potential of -60 mV using the whole-cell configuration of the patch-clamp technique with a List Electronics EPC-7 amplifier. ..

    Article Title: GluN2A-mediated currents and calcium signal in human iPSC-derived neurons
    Article Snippet: .. Membrane currents elicited by NMDA (TOCRIS, HB0454) and Glycine (Millipore Sigma, 106-57-0) were recorded at a membrane potential of −60 mV using the whole-cell configuration of the patch-clamp technique with a List Electronics EPC-7 amplifier. ..

    Patch Clamp:

    Article Title: GluN2A-mediated currents and calcium signal in human iPSC-derived neurons
    Article Snippet: .. Membrane currents elicited by NMDA (TOCRIS, HB0454) and Glycine (Millipore Sigma, 106-57-0) were recorded at a membrane potential of -60 mV using the whole-cell configuration of the patch-clamp technique with a List Electronics EPC-7 amplifier. ..

    Article Title: GluN2A-mediated currents and calcium signal in human iPSC-derived neurons.
    Article Snippet: .. Membrane currents elicited by NMDA (TOCRIS, HB0454) and Glycine (Millipore Sigma, 106-57-0) were recorded at a membrane potential of -60 mV using the whole-cell configuration of the patch-clamp technique with a List Electronics EPC-7 amplifier. ..

    Article Title: GluN2A-mediated currents and calcium signal in human iPSC-derived neurons
    Article Snippet: .. Membrane currents elicited by NMDA (TOCRIS, HB0454) and Glycine (Millipore Sigma, 106-57-0) were recorded at a membrane potential of −60 mV using the whole-cell configuration of the patch-clamp technique with a List Electronics EPC-7 amplifier. ..

    other:

    Article Title: Hyperexcitability in Alzheimer’s Disease triggers a compensatory neuroprotective response via TREK1
    Article Snippet: In parallel, to directly stimulate individual signaling nodes, cultures were treated with pathway- specific agonists in the absence of Aβ42, including a glutamate:glycine mixture (100 μM glutamate from Sigma-Aldrich and 10 μM glycine; 10:1 ratio), NMDA (100 μM; Tocris), KCl (25 mM; Fisher Scientific), forskolin (5 μM; Tocris), and the cAMP analog 8-CPT-cAMP (100 μM; Santa Cruz Biotechnology).

    Imaging:

    Article Title: Synaptic pruning following NMDAR-dependent LTD preferentially affects isolated synapses
    Article Snippet: .. For confocal imaging, primary neuronal cultures were treated either with 30 μM NMDA (Tocris) for 3 minutes or with 100 μM ATP in the presence of CGS15943 (3 μM) (Sigma-Aldrich) for 1 minute and fixed with PFA 30 minutes,3, 6 or 12 hours after, as precised in the figure. ..

    Article Title: Synaptic pruning following NMDAR-dependent LTD preferentially affects isolated synapses
    Article Snippet: .. For live imaging of EGFP-transfected primary neuronal cultures and synaptogamin-1 uptake measurement, neurons were treated with 30 μM NMDA (Tocris) for 3 minutes. .. After 30 minutes of incubation, they were placed in a Ludin chamber with culture media from their original dish, and a fluorescently labelled monoclonal mouse anti-synaptotagmin-1 (Synaptic System, 105311CpH) was applied in the bath at 1/200 for 30 minutes.



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    ( A ) A brightfield (BF) image of the neuronal culture on day 7, showing established cellular density and neurite outgrowth. Scale bar = 100 μm. ( B ) Immunofluorescence images of the culture at the same time point. From left to right: NfL (green) highlighting axonal structures; DAPI (blue) indicating nuclear localisation; and a merged overlay. Scale bar = 100 μm. ( C ) NfL concentration in the samples, collected at various time points (10 min, 60 min, 24 h) following drug administration, measured by ELISA. Cells were treated with staurosporine (100k cells per well) or <t>NMDA</t> (100k, 150k, 250k cells per well). Data are represented as the mean. All points beyond 400 pg mL −1 of NfL are estimated. ( D ) Tukey’s box plots of the S norm obtained by CV (red, left y -axis), EIS (orange), and CA (blue; both on the right y -axis) for blanks in two matrices—PBS and BP—and three groups of cell culture samples (at low, medium, and high concentration) in BP; separated data points were considered outliers.
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    ( A ) A brightfield (BF) image of the neuronal culture on day 7, showing established cellular density and neurite outgrowth. Scale bar = 100 μm. ( B ) Immunofluorescence images of the culture at the same time point. From left to right: NfL (green) highlighting axonal structures; DAPI (blue) indicating nuclear localisation; and a merged overlay. Scale bar = 100 μm. ( C ) NfL concentration in the samples, collected at various time points (10 min, 60 min, 24 h) following drug administration, measured by ELISA. Cells were treated with staurosporine (100k cells per well) or <t>NMDA</t> (100k, 150k, 250k cells per well). Data are represented as the mean. All points beyond 400 pg mL −1 of NfL are estimated. ( D ) Tukey’s box plots of the S norm obtained by CV (red, left y -axis), EIS (orange), and CA (blue; both on the right y -axis) for blanks in two matrices—PBS and BP—and three groups of cell culture samples (at low, medium, and high concentration) in BP; separated data points were considered outliers.
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    ( A ) A brightfield (BF) image of the neuronal culture on day 7, showing established cellular density and neurite outgrowth. Scale bar = 100 μm. ( B ) Immunofluorescence images of the culture at the same time point. From left to right: NfL (green) highlighting axonal structures; DAPI (blue) indicating nuclear localisation; and a merged overlay. Scale bar = 100 μm. ( C ) NfL concentration in the samples, collected at various time points (10 min, 60 min, 24 h) following drug administration, measured by ELISA. Cells were treated with staurosporine (100k cells per well) or <t>NMDA</t> (100k, 150k, 250k cells per well). Data are represented as the mean. All points beyond 400 pg mL −1 of NfL are estimated. ( D ) Tukey’s box plots of the S norm obtained by CV (red, left y -axis), EIS (orange), and CA (blue; both on the right y -axis) for blanks in two matrices—PBS and BP—and three groups of cell culture samples (at low, medium, and high concentration) in BP; separated data points were considered outliers.
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    Image Search Results


    ( A ) A brightfield (BF) image of the neuronal culture on day 7, showing established cellular density and neurite outgrowth. Scale bar = 100 μm. ( B ) Immunofluorescence images of the culture at the same time point. From left to right: NfL (green) highlighting axonal structures; DAPI (blue) indicating nuclear localisation; and a merged overlay. Scale bar = 100 μm. ( C ) NfL concentration in the samples, collected at various time points (10 min, 60 min, 24 h) following drug administration, measured by ELISA. Cells were treated with staurosporine (100k cells per well) or NMDA (100k, 150k, 250k cells per well). Data are represented as the mean. All points beyond 400 pg mL −1 of NfL are estimated. ( D ) Tukey’s box plots of the S norm obtained by CV (red, left y -axis), EIS (orange), and CA (blue; both on the right y -axis) for blanks in two matrices—PBS and BP—and three groups of cell culture samples (at low, medium, and high concentration) in BP; separated data points were considered outliers.

    Journal: Biosensors

    Article Title: Electrochemical Detection of Neuronal Injury in Cell Culture Samples: A Cost-Effective Biosensor for Neurofilament Light Sensing

    doi: 10.3390/bios16040212

    Figure Lengend Snippet: ( A ) A brightfield (BF) image of the neuronal culture on day 7, showing established cellular density and neurite outgrowth. Scale bar = 100 μm. ( B ) Immunofluorescence images of the culture at the same time point. From left to right: NfL (green) highlighting axonal structures; DAPI (blue) indicating nuclear localisation; and a merged overlay. Scale bar = 100 μm. ( C ) NfL concentration in the samples, collected at various time points (10 min, 60 min, 24 h) following drug administration, measured by ELISA. Cells were treated with staurosporine (100k cells per well) or NMDA (100k, 150k, 250k cells per well). Data are represented as the mean. All points beyond 400 pg mL −1 of NfL are estimated. ( D ) Tukey’s box plots of the S norm obtained by CV (red, left y -axis), EIS (orange), and CA (blue; both on the right y -axis) for blanks in two matrices—PBS and BP—and three groups of cell culture samples (at low, medium, and high concentration) in BP; separated data points were considered outliers.

    Article Snippet: N-methyl-D-aspartate (NMDA) was obtained from Tocris Bioscience (Bristol, UK).

    Techniques: Immunofluorescence, Concentration Assay, Enzyme-linked Immunosorbent Assay, Cell Culture