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rabbit polyclonal anti nlrp1 12256 1 ap  (Proteintech)


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    Structured Review

    Proteintech rabbit polyclonal anti nlrp1 12256 1 ap
    Rabbit Polyclonal Anti Nlrp1 12256 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nlrp1/NLRP1+Antibody/us12570735-950-93-92
    Average 93 stars, based on 24 article reviews
    rabbit polyclonal anti nlrp1 12256 1 ap - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: H3K18 lactylation-mediated SPHK1-SIRT1 feedback loop accelerates pyroptosis of tubular epithelial cells in sepsis-associated acute kidney injury
    Article Snippet: .. After blocking the membranes with TBST buffer containing 5% skim milk or 5% BSA, the membranes were incubated at 4 °C overnight with primary antibodies against SPHK1 (BA2865, Boster), KIM-1 (NBP1-76701, Novus), GSDMD (39754, CST), Caspase-1 (83383, CST), H3K18la (PTM-1427RM, PTM Bio), Cleaved Caspase-1 (89332, CST), P300 (ab275378, Abcam), Cleaved Caspase-1 (4199, CST), NLRP3 (P60622R3, Abmart), SIRT1 (13161-1-AP, Proteintech), NLRC4 (PB0658, Boster), PGC-1α (66369-1-Ig, Proteintech), Acetylated-Lysine (9441, CST), p-SIRT1 (SAB4301426, MilliporeSigma), Pan-Kla (PTM-1401RM, PTM Bio), Pan-Ubiquitin (PTM-1124RM, PTM Bio), AIM2 (20590-1-AP, Proteintech), NLRP1 (12256-1-AP, Proteintech), Histone H3 (PTM-1002RM, PTM Bio) and β-actin (AC026, Abclonal). ..

    Article Title: Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosis
    Article Snippet: .. After blocking using normal donkey serum (NDS, 5%) for 1 h sciatic and trigeminal nerve tissue were incubated with the following primary antibodies: F4/80 (#MA516624, rat monoclonal (Cl:A3-1), Thermo Fisher Scientific, 1:50, RRID:AB_253820), C5aR1 (#orb393229, rabbit polyclonal, Biorbyt Ltd, 1:100), C5aR1 (#MCA2456GA, rat monoclonal, Bio-Rad, 1:100, RRID:AB_770091), NLRP3 (#MA5-32255, rabbit monoclonal, Thermo Fisher Scientific, 1:100, RRID:AB_2809541), NLRP1 (#12256-1-AP, rabbit polyclonal, Proteintech, 1:50, RRID:AB_2298504) and IL-1β (#NB600-633, rabbit polyclonal, Novus Biological, 1:100, RRID:AB_10001060) for 1 h at room temperature and O/N at 4 °C with SOX10 (#AF2864, goat polyclonal, R&D Systems, 1:200, RRID:AB_442208). .. Sections were then incubated for 2 h in the dark with the following with fluorescent secondary antibodies: Alexa Fluor 488 (#A32790, donkey anti-rabbit polyclonal, Thermo Fisher Scientific, 1:600, RRID:AB_2762833), Alexa Fluor 488 (#A21208, donkey anti-rat polyclonal, Thermo Fisher Scientific, 1:600, RRID:AB_2535794) and Alexa Fluor 594 (#A11058, donkey anti-goat polyclonal, Thermo Fisher Scientific, 1:600, RRID:AB_2534105).

    Article Title: Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosis.
    Article Snippet: .. After blocking using normal donkey serum (NDS, 5%) for 1 h sciatic and trigeminal nerve tissue were incubated with the following primary antibodies: F4/80 (#MA516624, rat monoclonal (Cl:A3-1), Thermo Fisher Scientific, 1:50, RRID:AB_253820), C5aR1 (#orb393229, rabbit polyclonal, Biorbyt Ltd, 1:100), C5aR1 (#MCA2456GA, rat monoclonal, Bio-Rad, 1:100, RRID:AB_770091), NLRP3 (#MA5-32255, rabbit monoclonal, Thermo Fisher Scientific, 1:100, RRID:AB_2809541), NLRP1 (#12256-1-AP, rabbit polyclonal, Proteintech, 1:50, RRID:AB_2298504) and IL-1β (#NB600-633, rabbit polyclonal, Novus Biological, 1:100, RRID:AB_10001060) for 1 h at room temperature and O/N at 4 °C with SOX10 (#AF2864, goat polyclonal, R&D Systems, 1:200, RRID:AB_442208). .. Sections were then incubated for 2h in the dark with the following with fluorescent secondary antibodies: Alexa Fluor 488 (#A32790, donkey anti-rabbit polyclonal, Thermo Fisher Scientific, 1:600, RRID:AB_2762833), Alexa Fluor 488 (#A21208, donkey anti-rat polyclonal, Thermo Fisher Scientific, 1:600, RRID:AB_2535794) and Alexa Fluor 594 (#A11058, donkey anti-goat polyclonal, Thermo Fisher Scientific, 1:600, RRID:AB_2534105).

    Incubation:

    Article Title: H3K18 lactylation-mediated SPHK1-SIRT1 feedback loop accelerates pyroptosis of tubular epithelial cells in sepsis-associated acute kidney injury
    Article Snippet: .. After blocking the membranes with TBST buffer containing 5% skim milk or 5% BSA, the membranes were incubated at 4 °C overnight with primary antibodies against SPHK1 (BA2865, Boster), KIM-1 (NBP1-76701, Novus), GSDMD (39754, CST), Caspase-1 (83383, CST), H3K18la (PTM-1427RM, PTM Bio), Cleaved Caspase-1 (89332, CST), P300 (ab275378, Abcam), Cleaved Caspase-1 (4199, CST), NLRP3 (P60622R3, Abmart), SIRT1 (13161-1-AP, Proteintech), NLRC4 (PB0658, Boster), PGC-1α (66369-1-Ig, Proteintech), Acetylated-Lysine (9441, CST), p-SIRT1 (SAB4301426, MilliporeSigma), Pan-Kla (PTM-1401RM, PTM Bio), Pan-Ubiquitin (PTM-1124RM, PTM Bio), AIM2 (20590-1-AP, Proteintech), NLRP1 (12256-1-AP, Proteintech), Histone H3 (PTM-1002RM, PTM Bio) and β-actin (AC026, Abclonal). ..

    Article Title: Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosis
    Article Snippet: .. After blocking using normal donkey serum (NDS, 5%) for 1 h sciatic and trigeminal nerve tissue were incubated with the following primary antibodies: F4/80 (#MA516624, rat monoclonal (Cl:A3-1), Thermo Fisher Scientific, 1:50, RRID:AB_253820), C5aR1 (#orb393229, rabbit polyclonal, Biorbyt Ltd, 1:100), C5aR1 (#MCA2456GA, rat monoclonal, Bio-Rad, 1:100, RRID:AB_770091), NLRP3 (#MA5-32255, rabbit monoclonal, Thermo Fisher Scientific, 1:100, RRID:AB_2809541), NLRP1 (#12256-1-AP, rabbit polyclonal, Proteintech, 1:50, RRID:AB_2298504) and IL-1β (#NB600-633, rabbit polyclonal, Novus Biological, 1:100, RRID:AB_10001060) for 1 h at room temperature and O/N at 4 °C with SOX10 (#AF2864, goat polyclonal, R&D Systems, 1:200, RRID:AB_442208). .. Sections were then incubated for 2 h in the dark with the following with fluorescent secondary antibodies: Alexa Fluor 488 (#A32790, donkey anti-rabbit polyclonal, Thermo Fisher Scientific, 1:600, RRID:AB_2762833), Alexa Fluor 488 (#A21208, donkey anti-rat polyclonal, Thermo Fisher Scientific, 1:600, RRID:AB_2535794) and Alexa Fluor 594 (#A11058, donkey anti-goat polyclonal, Thermo Fisher Scientific, 1:600, RRID:AB_2534105).

    Article Title: Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosis.
    Article Snippet: .. After blocking using normal donkey serum (NDS, 5%) for 1 h sciatic and trigeminal nerve tissue were incubated with the following primary antibodies: F4/80 (#MA516624, rat monoclonal (Cl:A3-1), Thermo Fisher Scientific, 1:50, RRID:AB_253820), C5aR1 (#orb393229, rabbit polyclonal, Biorbyt Ltd, 1:100), C5aR1 (#MCA2456GA, rat monoclonal, Bio-Rad, 1:100, RRID:AB_770091), NLRP3 (#MA5-32255, rabbit monoclonal, Thermo Fisher Scientific, 1:100, RRID:AB_2809541), NLRP1 (#12256-1-AP, rabbit polyclonal, Proteintech, 1:50, RRID:AB_2298504) and IL-1β (#NB600-633, rabbit polyclonal, Novus Biological, 1:100, RRID:AB_10001060) for 1 h at room temperature and O/N at 4 °C with SOX10 (#AF2864, goat polyclonal, R&D Systems, 1:200, RRID:AB_442208). .. Sections were then incubated for 2h in the dark with the following with fluorescent secondary antibodies: Alexa Fluor 488 (#A32790, donkey anti-rabbit polyclonal, Thermo Fisher Scientific, 1:600, RRID:AB_2762833), Alexa Fluor 488 (#A21208, donkey anti-rat polyclonal, Thermo Fisher Scientific, 1:600, RRID:AB_2535794) and Alexa Fluor 594 (#A11058, donkey anti-goat polyclonal, Thermo Fisher Scientific, 1:600, RRID:AB_2534105).

    Pyrolysis Gas Chromatography:

    Article Title: H3K18 lactylation-mediated SPHK1-SIRT1 feedback loop accelerates pyroptosis of tubular epithelial cells in sepsis-associated acute kidney injury
    Article Snippet: .. After blocking the membranes with TBST buffer containing 5% skim milk or 5% BSA, the membranes were incubated at 4 °C overnight with primary antibodies against SPHK1 (BA2865, Boster), KIM-1 (NBP1-76701, Novus), GSDMD (39754, CST), Caspase-1 (83383, CST), H3K18la (PTM-1427RM, PTM Bio), Cleaved Caspase-1 (89332, CST), P300 (ab275378, Abcam), Cleaved Caspase-1 (4199, CST), NLRP3 (P60622R3, Abmart), SIRT1 (13161-1-AP, Proteintech), NLRC4 (PB0658, Boster), PGC-1α (66369-1-Ig, Proteintech), Acetylated-Lysine (9441, CST), p-SIRT1 (SAB4301426, MilliporeSigma), Pan-Kla (PTM-1401RM, PTM Bio), Pan-Ubiquitin (PTM-1124RM, PTM Bio), AIM2 (20590-1-AP, Proteintech), NLRP1 (12256-1-AP, Proteintech), Histone H3 (PTM-1002RM, PTM Bio) and β-actin (AC026, Abclonal). ..



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