nkcc  (OriGene)

Journal: Scientific Reports

Article Title: Experimental study on the effect of chlorhexidine gluconate (CG)-induced atrial fibrillation on renal water and sodium metabolism

doi: 10.1038/s41598-023-30783-w

Figure Lengend Snippet: Effects of acute atrial fibrillation on renal water channels and sodium transporters in mice. ( A ) Results of Western blotting detecting NKCC, ENaC-β, ENaC-γ, SGK1, AQP4, AQP3 and AQP2 expressions in kidneys in CON and AF. ( B ) Comparison of the expression of NKCC in kidneys in CON and AF. ( C ) Comparison of the expression of ENaC-β in kidneys in CON and AF. ( D ) Comparison of the expression of ENaC-γ in kidneys in CON and AF. ( E ) Comparison of the expression of SGK1 in kidneys in CON and AF. ( F ) Comparison of the expression of AQP4 in kidneys in CON and AF. ( G ) Comparison of the expression of AQP3 in kidneys in CON and AF. ( H ) Comparison of the expression of AQP2 in kidneys in CON and AF.

Article Snippet: The main reagents are chlorhexidine gluconate stock solution (McLean), pentobarbital (provided by the Chemistry Center Laboratory of Hebei Medical University), CRP and IL-8 ELISA kit (Beijing Solarbio company), immunohistochemical kit ( Beijing Zhongshan Jinqiao), BCA protein quantification kit (Beijing Solarbio Company), rabbit anti-GAPDH (Servicebio Company), rabbit anti-TGF-β (Servicebio Company), rabbit anti-NF-κB(Genetex Company), rabbit anti-collagen III (Servicebio Company), rabbit Anti-NKCC (Abclonal Company), rabbit anti-ENaC-α (Stressmarq Company), rabbit anti-ENaC-γ (Affiniti Company), rabbit anti-SGK1 (Huaan Biotechnology Company), rabbit anti-AQP2 (Abcam Company), rabbit anti-AQP3 (Abclonal Company), rabbit anti-AQP4 (Proteintech).

Techniques: Western Blot, Expressing

Journal: PLoS ONE

Article Title: High Brain Ammonia Tolerance and Down-Regulation of Na + :K + :2Cl - Cotransporter 1b mRNA and Protein Expression in the Brain of the Swamp Eel, Monopterus albus , Exposed to Environmental Ammonia or Terrestrial Conditions

doi: 10.1371/journal.pone.0069512

Figure Lengend Snippet: The percentage similarity between the deduced amino acid sequence Na + :K + :2Cl - cotransporter 1b (Nkcc1b) from the brain of Monopterus albus and Nkcc/NKCC sequences of other animal species obtained from GenBank (accession numbers in brackets).

Article Snippet: After transfer, membranes were blocked with 10% skim milk in TTBS (0.05% Tween 20 in Tris-buffered saline: 20 mmol l -1 Tris-HCl; 500 mmol l -1 NaCl, pH 7.6) for 1 h before being incubated overnight at 4°C with anti-NKCC antibody (T4, 1: 500 dilution) or pan-actin antibody (1:5000 dilution; Thermo Fisher Scientific Inc.).

Techniques: Sequencing