Journal: Cell Reports Medicine
Article Title: Adenosine A2A receptor is a tumor suppressor of NASH-associated hepatocellular carcinoma
doi: 10.1016/j.xcrm.2023.101188
Figure Lengend Snippet: Pharmacological blockade of A2AR signaling promotes HFD-induced obesity and myeloid-derived liver inflammation (A) Growth curve. WT male mice were fed with a CD-HFD and treated daily with KW6002 (10 mg/kg p.o.), CGS21680 (0.5 mg/kg i.p.), or vehicle for a total of 8 weeks. Mice weight was monitored every week. (B) After 8 weeks of treatment, fat mass was measured using an EchoMRI. (C) Growth curve of male C57BL/6 mice fed with a CD-HFD and treated daily with NIR178 (10 mg/kg p.o.) or vehicle. (D) Fat mass measurement after 8 weeks of treatment with NIR178 using an EchoMRI. (E–L) Livers of CD-HFD-fed mice treated with KW6002, CGS21608, or vehicle were collected, and immune infiltrate was analyzed by flow cytometry: (E) CD11b+ myeloid cells, (F) CD11b+ F4/80+ macrophages, (G) CD11b+ Ly6G-Ly6C low monocytes, (H) CD11b+ Ly6G- Ly6C high inflammatory monocytes, (I) CD11b+ Ly6G+ neutrophils, (J) TCRb+ CD4+ T cells, (K) TCRb+ CD8+ T cells, and (L) TCRb+ CD11b+ NKT cells. (M and N) Real-time PCR showing mRNA level of liver-derived cytokines and lipogenesis markers from treated mice. Results are representative of 1 biological replicate, and sample size is indicated for each panel. Data are presented as means ± SEM. Statistical tests: Student’s t test (C and D) or multiple t tests with Benjamin-Hochberg correction (A, B, and E–N).
Article Snippet: 8-10 weeks old C57BL/6J males (Jackson Lab) received daily administrations of KW6002 (Istradefylline, Toronto Research Chemicals), NIR178 (Taminadenant, Selleckchem) or CGS21680 (Selleckchem).
Techniques: Derivative Assay, Mass Measurement, Flow Cytometry, Real-time Polymerase Chain Reaction
