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nimodipine  (Tocris)


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    Structured Review

    Tocris nimodipine
    Cells were pretreated without or with IGF-1 (10 ng/mL) followed by the L-type calcium channel blocker <t>nimodipine</t> (5 µM) and the NMDAR antagonist APV (50 µM). After 3 minutes, they were stimulated with AMPA. Representative traces show that IGF-1 attenuates AMPA-evoked [Ca 2+ ] i elevation ( P <0.05; Student’s t test) independent of L-type calcium channels.
    Nimodipine, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nimodipine/Nimodipine/bio_rxiv__64898__2026__04__04__716504-121-19-20
    Average 95 stars, based on 256 article reviews
    nimodipine - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Insulin Growth Factor 1 affects glutamate receptor activity differently in primary cultures of neocortical versus hippocampal neurons"

    Article Title: Insulin Growth Factor 1 affects glutamate receptor activity differently in primary cultures of neocortical versus hippocampal neurons

    Journal: bioRxiv

    doi: 10.64898/2026.04.04.716504

    Cells were pretreated without or with IGF-1 (10 ng/mL) followed by the L-type calcium channel blocker nimodipine (5 µM) and the NMDAR antagonist APV (50 µM). After 3 minutes, they were stimulated with AMPA. Representative traces show that IGF-1 attenuates AMPA-evoked [Ca 2+ ] i elevation ( P <0.05; Student’s t test) independent of L-type calcium channels.
    Figure Legend Snippet: Cells were pretreated without or with IGF-1 (10 ng/mL) followed by the L-type calcium channel blocker nimodipine (5 µM) and the NMDAR antagonist APV (50 µM). After 3 minutes, they were stimulated with AMPA. Representative traces show that IGF-1 attenuates AMPA-evoked [Ca 2+ ] i elevation ( P <0.05; Student’s t test) independent of L-type calcium channels.

    Techniques Used:

    Related Articles

    Blocking Assay:

    Article Title: Synapses drive local mitochondrial ATP synthesis to fuel plasticity
    Article Snippet: .. For the NMDAR block experiment, neurons were treated with 50 μM APV (Tocris, 100 mM stock made in modified E4 buffer lacking Mg 2+ ) for at least 5 min. For the L-type calcium channel block experiment, neurons were treated with 10 μM Nimodipine (Tocris, 100 mM stock made in DMSO) for at least 10 min. For SERCA block experiments, neurons were treated with 1 μM Thapsigargin (Tocris, 1 mM stock made in DMSO) or 50 μM of CPA (Alomone, 50 mM stock made in DMSO) for at least 10 min. For RyR block experiments, neurons were treated with 100 μM Ryanodine (Tocris, 25 mM stock made in DMSO) for at least 5 min. For IP3R block experiments, neurons were treated with 3 μM XestC (Tocris, 100 μM stock made in DMSO) for at least 40 min. ..

    Modification:

    Article Title: Synapses drive local mitochondrial ATP synthesis to fuel plasticity
    Article Snippet: .. For the NMDAR block experiment, neurons were treated with 50 μM APV (Tocris, 100 mM stock made in modified E4 buffer lacking Mg 2+ ) for at least 5 min. For the L-type calcium channel block experiment, neurons were treated with 10 μM Nimodipine (Tocris, 100 mM stock made in DMSO) for at least 10 min. For SERCA block experiments, neurons were treated with 1 μM Thapsigargin (Tocris, 1 mM stock made in DMSO) or 50 μM of CPA (Alomone, 50 mM stock made in DMSO) for at least 10 min. For RyR block experiments, neurons were treated with 100 μM Ryanodine (Tocris, 25 mM stock made in DMSO) for at least 5 min. For IP3R block experiments, neurons were treated with 3 μM XestC (Tocris, 100 μM stock made in DMSO) for at least 40 min. ..

    Control:

    Article Title: Intercellular communication in the brain via dendritic nanotubular network
    Article Snippet: .. Depending on the experiment, 0.1% DMSO (Thermo Fisher Scientific), 1 μM Cytochalasin-D (Sigma-Aldrich, Cat.#: C2618), 100 μM Carbenoxolone (Sigma-Aldrich, Cat.#: C4790), 50 μM BAPTA-AM (Tocris, Minneapolis, MN, USA, Cat.#: HY-100545), 100 μM D-AP5 (Tocris, Cat.#: 0106), 20 μM Nimodipine (Tocris, Cat.#: 0600), 30 μM Trovafloxacin (Tocris, Cat.#: 3863) was added with or without 1 μM Tetrodotoxin citrate (Tocris, Cat.#: 1069) while the amount of DMSO for the control group was set to match the amount of Cyt-D or CBX. ..

    other:

    Article Title: Behavioral inflexibility through overtraining is mediated by reduced mGluR1/5 signaling capacity in the dorsolateral striatum
    Article Snippet: AM251, (RS)-3,5-DHPG, Methyl-6-(phenylethynyl)pyridine hydrochloride (MPEP), NBQX disodium salt, Ro25-6981 maleate, SR 95531 hydrobromide (Gabazine), tetrodotoxin (TTx) and Nimodipine were purchased from Tocris Bioscience (Avonmouth, UK).

    Article Title: Ca V 1.2-dependent excitation-transcription coupling modulates nociception
    Article Snippet: Stock solutions were prepared as indicated in parentheses of the following compounds: Autocamtide-2 Related Inhibitory Peptide (AIP, Calbiochem, #189480, 500 μM in dH2O), (S)-(-)-Bay K 8644 (BayK, Alomone Labs, #B-351, 50 mM in DMSO), Capsaicin (Cap, Sigma-Aldrich, #M2028, 10 mM in DMSO), Cyclosporin A (CsA, Tocris, #1101, 50 mM in DMSO), H-89 Dihydrochloride (H89, Tocris, #2910, 25 mM in DMSO), KN93 (Tocris, #1278; 20 mM in DMSO), Nifedipine (Nife, Tocris, #1075, 10 mM in DMSO), Nimodipine (Nimo, Tocris, #0600, 10 mM in DMSO), STO-609 (Tocris, #1551, 10 mM in DMSO), U0126 (Calbiochem, #662005, 50 mM in DMSO), Verapamil (VP, Alomone Labs, #V-100, 100 mM stock in dH2O), Veratridine (VT, Alomone Labs; #V-110; 50 mM stock in DMSO), ω-agatoxin IVA (Alomone Labs, #STA-500, 200 μM stock in 0.1% BSA), ω-conotoxin GVIA (Alomone Labs, # C-300, 1 mM stock in 0.1% BSA), ω-conotoxin MVIIC (Alomone Labs, #C-150, 300 μM stock in 0.1% BSA).

    Article Title: Activity-dependent localization and dynamics of STIM1 and STIM2 at ER-PM contacts in hippocampal neurons.
    Article Snippet: K V 2.1 (NeuroMab, 75–159), MAP2 (Synaptic Systems, 188011, 188004), NR2B (Alomone Labs, AGC-003), STIM1 (Sigma-Aldrich, S6072), STIM2 (Alomone Labs, ACC-064), and Halo-Tag 646 (Promega, G9281), Fura-2 a.m. (abcam, ab120873), HRP anti-Guinea Pig (Jackson IR, 706-035-148) and HRP anti-Mouse (Jackson IR, 115-035-146) Pharmacological agents used in this study: DHPG (HelloBio, HB0045), Bicuculline Methiodide (Tocris, 2503), Caffeine (SigmaAldrich, C0750), CNQX Disodium Salt (HelloBio/Tocris, HB0204/1045), Cyclopiazonic Acid (CPA) (HelloBio, HB1117), D-AP5/DAPV (HelloBio/Tocris, HB0225/0106), Ifenprodil (HelloBio, HB0339), L-Glutamate (abcam, ab120049), MK 801 Maleate (HelloBio, HB0004), Nimodipine (Tocris, 0600/100), NMDA (N-Methyl-D-Aspartic Acid) (HelloBio, HB0454), Ryanodine (HelloBio, HB1320), and TG (Thapsigargin) (HelloBio, HB1118), Bay K 8644 (Torcis, 1544).

    Article Title: Behavioral inflexibility through overtraining is mediated by reduced mGluR1/5 signaling capacity in the dorsolateral striatum.
    Article Snippet: AM251, (RS)-3,5-DHPG, Methyl-6-(phenylethynyl)pyridine hydrochloride (MPEP), NBQX disodium salt, Ro25-6981 maleate, SR 95531 hydrobromide (Gabazine), tetrodotoxin (TTx) and Nimodipine were purchased from Tocris Bioscience (Avonmouth, UK).

    Article Title: Activity-Dependent Localization and Heterogeneous Dynamics of STIM1 and STIM2 at ER-PM contacts in Hippocampal Neurons
    Article Snippet: Pharmacological agents used in this study: DHPG (Hel-loBio, HB0045), Bicuculline Methiodide (Tocris, 2503), Caffeine (Sigma-Aldrich, C0750), CNQX Disodium Salt (HelloBio/Tocris, HB0204/1045), Cyclopiazonic Acid (CPA) (HelloBio, HB1117), D-AP5/D-APV (HelloBio/Tocris, HB0225/0106), Ifenprodil (HelloBio, HB0339), L-Glutamate (abcam, ab120049), MK 801 Maleate (HelloBio, HB0004), Nimodipine (Tocris, 0600/100), NMDA (N-Methyl-D-Aspartic Acid) (HelloBio, HB0454), Ryanodine (HelloBio, HB1320), and TG (Thapsigargin) (HelloBio, HB1118).

    Isolation:

    Article Title: Insulin Growth Factor 1 affects glutamate receptor activity differently in primary cultures of neocortical versus hippocampal neurons
    Article Snippet: .. In the pharmacological isolation of evoked currents, the drugs used included the AMPA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; Tocris) and nimodipine (Tocris), an L-type calcium channel blocker, dissolved in dimethyl sulfoxide (DMSO) to create a stock solution, ensuring the final DMSO concentration was below 0.05%. .. Glutamate (Millipore-Sigma), glycine (Fisher), and KCl (Fisher) were all prepared as stock solutions using deionized water.

    Concentration Assay:

    Article Title: Insulin Growth Factor 1 affects glutamate receptor activity differently in primary cultures of neocortical versus hippocampal neurons
    Article Snippet: .. In the pharmacological isolation of evoked currents, the drugs used included the AMPA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; Tocris) and nimodipine (Tocris), an L-type calcium channel blocker, dissolved in dimethyl sulfoxide (DMSO) to create a stock solution, ensuring the final DMSO concentration was below 0.05%. .. Glutamate (Millipore-Sigma), glycine (Fisher), and KCl (Fisher) were all prepared as stock solutions using deionized water.



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    Shortening of action potential duration in HL‐1 cells following rapid electrical stimulation. (A) Representative action potential traces recorded from HL‐1 cells under control conditions and after 2 days of rapid pacing at 300 bpm; the recording frequency was 1 Hz. (B) Summary of APD 90 measurements at different stimulation frequencies, comparing control and paced groups. (C) Representative I Ca, L traces recorded via patch clamp, with voltage steps initiated from −90 mV (left) and −40 mV (right). (D) Dose–response curves of L‐type calcium current inhibition by varying concentrations of <t>nimodipine.</t> (E) Current–voltage (I–V) relationships under different holding potentials (−90 mV and −40 mV), illustrating voltage dependence. All experiments were repeated in triplicate ( n = 3 biological replicates). * p < 0.05.
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    Image Search Results


    Shortening of action potential duration in HL‐1 cells following rapid electrical stimulation. (A) Representative action potential traces recorded from HL‐1 cells under control conditions and after 2 days of rapid pacing at 300 bpm; the recording frequency was 1 Hz. (B) Summary of APD 90 measurements at different stimulation frequencies, comparing control and paced groups. (C) Representative I Ca, L traces recorded via patch clamp, with voltage steps initiated from −90 mV (left) and −40 mV (right). (D) Dose–response curves of L‐type calcium current inhibition by varying concentrations of nimodipine. (E) Current–voltage (I–V) relationships under different holding potentials (−90 mV and −40 mV), illustrating voltage dependence. All experiments were repeated in triplicate ( n = 3 biological replicates). * p < 0.05.

    Journal: Journal of Cell Communication and Signaling

    Article Title: P2X7 receptors promote atrial remodeling and atrial fibrillation susceptibility via reactive oxygen species‐mediated mitogen‐activated protein kinase signaling activation

    doi: 10.1002/ccs3.70071

    Figure Lengend Snippet: Shortening of action potential duration in HL‐1 cells following rapid electrical stimulation. (A) Representative action potential traces recorded from HL‐1 cells under control conditions and after 2 days of rapid pacing at 300 bpm; the recording frequency was 1 Hz. (B) Summary of APD 90 measurements at different stimulation frequencies, comparing control and paced groups. (C) Representative I Ca, L traces recorded via patch clamp, with voltage steps initiated from −90 mV (left) and −40 mV (right). (D) Dose–response curves of L‐type calcium current inhibition by varying concentrations of nimodipine. (E) Current–voltage (I–V) relationships under different holding potentials (−90 mV and −40 mV), illustrating voltage dependence. All experiments were repeated in triplicate ( n = 3 biological replicates). * p < 0.05.

    Article Snippet: Nimodipine (HY‐10350) was obtained from MedChemExpress (MCE, USA), prepared in 0.1% dimethyl sulfoxide (DMSO) as a stock solution, and then diluted to the desired concentrations for experimental use.

    Techniques: Control, Patch Clamp, Inhibition

    Cells were pretreated without or with IGF-1 (10 ng/mL) followed by the L-type calcium channel blocker nimodipine (5 µM) and the NMDAR antagonist APV (50 µM). After 3 minutes, they were stimulated with AMPA. Representative traces show that IGF-1 attenuates AMPA-evoked [Ca 2+ ] i elevation ( P <0.05; Student’s t test) independent of L-type calcium channels.

    Journal: bioRxiv

    Article Title: Insulin Growth Factor 1 affects glutamate receptor activity differently in primary cultures of neocortical versus hippocampal neurons

    doi: 10.64898/2026.04.04.716504

    Figure Lengend Snippet: Cells were pretreated without or with IGF-1 (10 ng/mL) followed by the L-type calcium channel blocker nimodipine (5 µM) and the NMDAR antagonist APV (50 µM). After 3 minutes, they were stimulated with AMPA. Representative traces show that IGF-1 attenuates AMPA-evoked [Ca 2+ ] i elevation ( P <0.05; Student’s t test) independent of L-type calcium channels.

    Article Snippet: In the pharmacological isolation of evoked currents, the drugs used included the AMPA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; Tocris) and nimodipine (Tocris), an L-type calcium channel blocker, dissolved in dimethyl sulfoxide (DMSO) to create a stock solution, ensuring the final DMSO concentration was below 0.05%.

    Techniques: