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nefm  (OriGene)


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    Structured Review

    OriGene nefm
    Nefm, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nefm/pm42189461-51-7-28?v=OriGene
    Average 94 stars, based on 1 article reviews
    nefm - by Bioz Stars, 2026-07
    94/100 stars

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    Image Search Results


    GMs promote maturation and biocompatibility in SN4741 cell cultures (7 days in culture). Nuclear staining with Hoechst 3258 (in vivo) shows no apoptotic bodies in adherent cells cultured on GM substrates. ( a1 ) GO film; ( b1 ) PRGO film; ( c1 ) FRGO film; ( d1 ) control. Merged images display neurofilament-M (NEFM) (green)/DAPI (blue) in ( a2 – d2 ) and neurofilament-H (NEFH) (red)/DAPI (blue) in ( a3 – d3 ) Immature cell marker DCX (red)/DAPI (blue) is shown in ( a4 – d4 ). Scale bars: 25 µm ( a1 , d1 , a2 – c2 , a3 , d3 , a4 , d4 ); 50 µm ( b1 , d2 , b4 , c4 ); 120 µm ( c1 ).

    Journal: International Journal of Molecular Sciences

    Article Title: Neuroprotective Effects of Low-Dose Graphenic Materials on SN4741 Embryonic Stem Cells Against ER Stress and MPTP-Induced Oxidative Stress

    doi: 10.3390/ijms26188821

    Figure Lengend Snippet: GMs promote maturation and biocompatibility in SN4741 cell cultures (7 days in culture). Nuclear staining with Hoechst 3258 (in vivo) shows no apoptotic bodies in adherent cells cultured on GM substrates. ( a1 ) GO film; ( b1 ) PRGO film; ( c1 ) FRGO film; ( d1 ) control. Merged images display neurofilament-M (NEFM) (green)/DAPI (blue) in ( a2 – d2 ) and neurofilament-H (NEFH) (red)/DAPI (blue) in ( a3 – d3 ) Immature cell marker DCX (red)/DAPI (blue) is shown in ( a4 – d4 ). Scale bars: 25 µm ( a1 , d1 , a2 – c2 , a3 , d3 , a4 , d4 ); 50 µm ( b1 , d2 , b4 , c4 ); 120 µm ( c1 ).

    Article Snippet: The following primary antibodies were used: polyclonal rabbit anti-tyrosine hydroxylase (TH, #NB300-109 Novus Biologicals, Centennial, CO 80112, USA); mouse anti-Tuj-1 (Anti-βIII Tubulin, clone 5G8, Promega Alcobendas, Madrid, Spain); polyclonal goat anti-GIRK2 (G-protein-regulated inward-rectifier potassium channel 2 protein, #ab65096; Abcam, Cambridge, Reino Unido); polyclonal rat anti-DAT (dopamine transporter, #MAB369 Millipore, Burlington, Massachusetts, United States); polyclonal rabbit anti-neurofilament-H NEFH (#ab8135); polyclonal rabbit anti-neurofilament M, NEFM (#25805-1-AP, Proteintech, Planegg-Martinsried Germany); monoclonal mouse anti-Doublecortin (DCX, #ab18723; Abcam); Hoechst 33,258 (live cells, PubmedChen #329770334, Bethesda, MD USA); and DAPI (Sigma-Aldrich, Saint Louis, MO 63103 United States).

    Techniques: Staining, In Vivo, Cell Culture, Control, Marker