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brca1 gene 1 nbr1 (Proteintech)

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Proteintech brca1 gene 1 nbr1
Brca1 Gene 1 Nbr1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nbr1/NBR1+Antibody/pm41595571-80-104-108
Average 94 stars, based on 72 article reviews

brca1 gene 1 nbr1 - by Bioz Stars, 2026-09

94/100 stars

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Incubation:

Article Title: Oxymatrine Alleviates Cerebral Ischemia/Reperfusion Injury By Targeting HDAC1 to Regulate Mitochondria-Related Autophagy and Oxidative Stress.
Article Snippet: Coronal sections underwent antigen retrieval using citrate buffer (Beyotime, Jiangsu, China), followed by blocking with 10% goat serum in PBS for 1 h at room temperature. .. Sections were incubated overnight at 4 °C with the following primary antibodies diluted in PBS:LC3 and PINK1 (1:200, ABclonal, Wuhan, China); Beclin1, Apaf1, NeuN, P62 and NBR1 (1:100, Proteintech, Wuhan, China).After three 5-min washes with PBS, sections were incubated with Alexa Fluor-conjugated secondary antibodies (1:500) at 37 °C for 1 h. Nuclei were counterstained with DAPI (Solarbio, Beijing, China) for 10 min at room temperature. ..

Article Title: CircRNA GRAMD4 induces NBR1 expression to promote autophagy and immune escape in renal cell carcinoma
Article Snippet: .. Next, incubation overnight using primary antibodies targeting MKI67/Ki67 (CST, 9129S), LC3B (CST 83,506), MHC-I (abcam, ab134189), NBR1 (Proteintech 16,004–1-AP) and RBM4 (Proteintech 11,614–1-AP) antibodies at 4°C overnight, and apply secondary antibodies the next day, followed by DAB staining, hematoxylin staining of cell nuclei, dehydration and sealing after differentiation. ..

Article Title: Oxymatrine Alleviates Cerebral Ischemia/Reperfusion Injury By Targeting HDAC1 to Regulate Mitochondria-Related Autophagy and Oxidative Stress
Article Snippet: Coronal sections underwent antigen retrieval using citrate buffer (Beyotime, Jiangsu, China), followed by blocking with 10% goat serum in PBS for 1 h at room temperature. .. Sections were incubated overnight at 4 °C with the following primary antibodies diluted in PBS:LC3 and PINK1 (1:200, ABclonal, Wuhan, China); Beclin1, Apaf1, NeuN, P62 and NBR1 (1:100, Proteintech, Wuhan, China).After three 5-min washes with PBS, sections were incubated with Alexa Fluor-conjugated secondary antibodies (1:500) at 37 °C for 1 h. Nuclei were counterstained with DAPI (Solarbio, Beijing, China) for 10 min at room temperature. ..

SDS Page:

Article Title: Oxymatrine Alleviates Cerebral Ischemia/Reperfusion Injury By Targeting HDAC1 to Regulate Mitochondria-Related Autophagy and Oxidative Stress.
Article Snippet: .. Protein extracts were separated on 10%–12% SDS-PAGE gels, transferred to PVDF membranes (Millipore), and probed with the following primary antibodies: Apaf-1 (1:1000, ABclonal, Wuhan, China), cleaved-caspase3 (1:500, Proteintech, Wuhan, China), Fis1 (1:2000, Proteintech, Wuhan, China), NBR1 (1:1000, Proteintech, Wuhan, China), Mfn2 (1:4000, Proteintech, Wuhan, China), LC3 (1:1000, ABclonal, Wuhan, China), HDAC1(1:2000, ABclonal, Wuhan, China), PINK1 (1:800, ABclonal, Wuhan, China), Parkin (1:2000, Proteintech, Wuhan, China), Beclin1 (1:2000, Proteintech, Wuhan, China), p62 (1:2000,Proteintech, Wuhan, China). .. And GAPDH (1:4000, Proteintech, Wuhan, China) was used as a loading control.

Article Title: Oxymatrine Alleviates Cerebral Ischemia/Reperfusion Injury By Targeting HDAC1 to Regulate Mitochondria-Related Autophagy and Oxidative Stress
Article Snippet: .. Protein extracts were separated on 10%–12% SDS-PAGE gels, transferred to PVDF membranes (Millipore), and probed with the following primary antibodies: Apaf-1 (1:1000, ABclonal, Wuhan, China), cleaved-caspase3 (1:500, Proteintech, Wuhan, China), Fis1 (1:2000, Proteintech, Wuhan, China), NBR1 (1:1000, Proteintech, Wuhan, China), Mfn2 (1:4000, Proteintech, Wuhan, China), LC3 (1:1000, ABclonal, Wuhan, China), HDAC1(1:2000, ABclonal, Wuhan, China), PINK1 (1:800, ABclonal, Wuhan, China), Parkin (1:2000, Proteintech, Wuhan, China), Beclin1 (1:2000, Proteintech, Wuhan, China), p62 (1:2000,Proteintech, Wuhan, China). .. And GAPDH (1:4000, Proteintech, Wuhan, China) was used as a loading control.

Immunopeptidomics:

Staining:

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$HEK293T cells were transfected with 2,000 ng of either Lab-Vpr or TFV-Vpr. 48 h later, cells were harvested, and lysates were immunoprecipitated for BNIP3L (A) , OPNT (B) , NBR1 (C) , SQSTM1 (D) , TAX1BP1 (E) , or NDP52 (F) . The pulldown fraction was analyzed for each of these autophagy receptors and Vpr. The whole cell lysates were also analyzed for these autophagy factors, Vpr and ACTB. Blots are representative of 3 independent experiments. (G) Lab-Vpr was transfected in parental, SQSTM1 KO / TAX1BP1 KO and SQSTM1 KO / TAX1BP1 KO / NDP52 KO knockout cells. 44 h later, cells were treated with increasing amounts of Torin2 (5-10 nM) for 4 h. Cells were then harvested and analyzed by western blot for Vpr, SQSTM1, TAX1BP1, NDP52 and ACTB. Blots are representative of 3 independent experiments. Graph: Vpr levels were quantified by normalizing to ACTB, and their relative expression compared to WT cells treated with DMSO (D on the x axis) were calculated from 3 independent experiments. Data correspond to the mean and the SEM of 3 independent experiments. *: p < 0.05.$

Journal: PLOS Pathogens

Article Title: HIV-1 Vpr is targeted for degradation by autophagy

doi: 10.1371/journal.ppat.1014020

Figure Lengend Snippet: HEK293T cells were transfected with 2,000 ng of either Lab-Vpr or TFV-Vpr. 48 h later, cells were harvested, and lysates were immunoprecipitated for BNIP3L (A) , OPNT (B) , NBR1 (C) , SQSTM1 (D) , TAX1BP1 (E) , or NDP52 (F) . The pulldown fraction was analyzed for each of these autophagy receptors and Vpr. The whole cell lysates were also analyzed for these autophagy factors, Vpr and ACTB. Blots are representative of 3 independent experiments. (G) Lab-Vpr was transfected in parental, SQSTM1 KO / TAX1BP1 KO and SQSTM1 KO / TAX1BP1 KO / NDP52 KO knockout cells. 44 h later, cells were treated with increasing amounts of Torin2 (5-10 nM) for 4 h. Cells were then harvested and analyzed by western blot for Vpr, SQSTM1, TAX1BP1, NDP52 and ACTB. Blots are representative of 3 independent experiments. Graph: Vpr levels were quantified by normalizing to ACTB, and their relative expression compared to WT cells treated with DMSO (D on the x axis) were calculated from 3 independent experiments. Data correspond to the mean and the SEM of 3 independent experiments. *: p < 0.05.

Article Snippet: NBR1 , Rabbit monoclonal (E6Q3F) to NBR1 , 1:1000 , Cell signaling, #20145S.

Techniques: Transfection, Immunoprecipitation, Knock-Out, Western Blot, Expressing