Journal: PLoS Pathogens
Article Title: Herpes simplex virus 1 evades cellular antiviral response by inducing microRNA-24, which attenuates STING synthesis
doi: 10.1371/journal.ppat.1009950
Figure Lengend Snippet: (A) (B) STING expression was analyzed by western blotting in human (A) HT1080 cells and (B) mouse NB41A3 cells upon HSV1 infection (MOI-5). (C) Figure depicting expression of miR-24 from 2 genetic loci. mir-24 is expressed in clusters from human chromosome 9 (miR-23b/ miR-27b/ miR-24-1) and chromosome 19 (miR-23a/ miR-27a/miR-24-2). Chromosome 9 cluster is expressed along with AOPEP mRNA. Human HT1080 cells were infected by HSV1 and cells were harvested at various time points. (D) miR-24, (E) miR-23b and (F) miR-27b expression levels were measured. (G) The expression of AOPEP mRNA was quantified. (H) Mouse NB41A3 cells were infected by HSV1 and cells were harvested at various time points to determine miR-24 levels. ( D, E, F, G, H : Mean ± SEM, N = 3). *P<0.05, **P<0.01, ***P<0.001.
Article Snippet: HT1080 WT (CCL-121), Vero cells and NB41A3 cells (CCL-147) were obtained from ATCC (Manassas, VA, USA).
Techniques: Expressing, Western Blot, Infection