nanowell chips icell8 250 v chip (TaKaRa)

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TaKaRa nanowell chips icell8 250 v chip
Nanowell Chips Icell8 250 V Chip, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nanowells/ICELL8+250v+Chip/pm41771837-390-6-14
Average 94 stars, based on 9 article reviews

nanowell chips icell8 250 v chip - by Bioz Stars, 2026-09

94/100 stars

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Polymerase Chain Reaction:

Article Title: Modeling glioblastoma invasion using human brain organoids and single-cell transcriptomics
Article Snippet: .. After thawing frozen chips, 50 nl of RT/Amp solution was dispensed into selected nanowells (Master mix: 56 μl 5 M Betaine, 24 μl 25 mM dNTP mix (TakaraBio), 3.2 μl 1 M MgCl 2 (Invitrogen), 8.8 μl 100 mM Dithiothreitol (TakaraBio), 61.9 μl 5x SMARTScribeTM first-strand buffer, 33.3 μl 2x SeqAmpTM PCR buffer, 4.0 μl 100 μM RT E5 Oligo, 8.8 μl 10 μM Amp primer (all TakaraBio), 1.6 μl 100% Triton X-100 (Acros), 28.8 μl SMARTScribeTM Reverse Transcriptase, 9.6 μl SeqAmpTM DNA Polymerase (TakaraBio)). .. After in-chip RT/Amp amplification (18 amplification cycles, in-chip RT/Amp Rapid Development protocol) inside a modified SmartChip Cycler (Bio-Rad), libraries were pooled, concentrated (DNA Clean and Concentrator−5 kit, Zymo Research) and purified using 0.6x Ampure XP beads.

Article Title: Modeling glioblastoma invasion using human brain organoids and single-cell transcriptomics
Article Snippet: .. After thawing frozen chips, 50 nl of RT/Amp solution was dispensed into selected nanowells (Master mix: 56 μl 5 M Betaine, 24 μl 25 mM dNTP mix (TakaraBio), 3.2 μl 1 M MgCl2 (Invitrogen), 8.8 μl 100 mM Dithiothreitol (TakaraBio), 61.9 μl 5x SMARTScribeTM first-strand buffer, 33.3 μl 2x SeqAmpTM PCR buffer, 4.0 μl 100 μM RT E5 Oligo, 8.8 μl 10 μM Amp primer (all TakaraBio), 1.6 μl 100% Triton X-100 (Acros), 28.8 μl SMARTScribeTM Reverse Transcriptase, 9.6 μl SeqAmpTM DNA Polymerase (TakaraBio)). .. After in-chip RT/Amp amplification (18 amplification cycles, in-chip RT/Amp Rapid Development protocol) inside a modified SmartChip Cycler (Bio-Rad), libraries were pooled, concentrated (DNA Clean and Concentrator−5 kit, Zymo Research) and purified using 0.6x Ampure XP beads.

Reverse Transcription:

Article Title: Modeling glioblastoma invasion using human brain organoids and single-cell transcriptomics
Article Snippet: .. After thawing frozen chips, 50 nl of RT/Amp solution was dispensed into selected nanowells (Master mix: 56 μl 5 M Betaine, 24 μl 25 mM dNTP mix (TakaraBio), 3.2 μl 1 M MgCl2 (Invitrogen), 8.8 μl 100 mM Dithiothreitol (TakaraBio), 61.9 μl 5x SMARTScribeTM first-strand buffer, 33.3 μl 2x SeqAmpTM PCR buffer, 4.0 μl 100 μM RT E5 Oligo, 8.8 μl 10 μM Amp primer (all TakaraBio), 1.6 μl 100% Triton X-100 (Acros), 28.8 μl SMARTScribeTM Reverse Transcriptase, 9.6 μl SeqAmpTM DNA Polymerase (TakaraBio)). .. After in-chip RT/Amp amplification (18 amplification cycles, in-chip RT/Amp Rapid Development protocol) inside a modified SmartChip Cycler (Bio-Rad), libraries were pooled, concentrated (DNA Clean and Concentrator−5 kit, Zymo Research) and purified using 0.6x Ampure XP beads.

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nanowell chips icell8 250 v chip (TaKaRa)

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