Journal: bioRxiv
Article Title: Feeder cell – the key component in producing scalable and fit NK cells for therapeutic use
doi: 10.64898/2026.04.21.718880
Figure Lengend Snippet: NK cell cytotoxicity against K562 target cells was assessed in a 24-hour co-culture assay at varying effector-to-target (E:T) ratios. NK cells were collected for the assay at multiple timepoints: starting immediately after thawing (A), and at weeks 1 (B) and 4 (C), as well as during each sample’s the week precedin the last week of survival lon -term culture (D). (E) Responsiveness to IL-2 stimulation was evaluated in thawed NK cells using a 24-hour co-culture assay with K562 cells immediately after thawing. 500 IU/ml IL-2 was either added (+) or omitted (–) from the co-culture to assess its effect on cytotoxic activity. (F) Cytotoxicity against NALM-6 cells and (G) SH-Sy5y cells were measured one week after thawing using 24-hour co-culture assays. Each dot represents an individual donor; bars indicate mean values. Data are presented as mean ± SD from (A) n = 3, (B) n = 6, (C) n = 3, (D) n = 3, (E) n = 3, (F) n = 6 and (G) n = 6 individual donors. Data (E) points represent individual donors: donor A (circles), B (triangles) and C (squares). Statistical analysis comparisons were made against the Day 1, non-expanded NK cell condition and in (E) by comparing IL-2 added and omitted condition, using two-tailed paired t-test. p < 0.05 (*), p < 0.01 (**), p < 0.001 (***).
Article Snippet: Briefly, NALM-6 cells (ATCC, cat# CRL-3273) were transduced with the IVISbrite Red F-luc-GFP lentiviral vector (RediFect, PerkinElmer) at an MOI of 10.
Techniques: Co-culture Assay, Co-Culture Assay, Activity Assay, Two Tailed Test