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n1-methyl-pseudoutp  (TriLink)

 
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    Structured Review

    TriLink n1-methyl-pseudoutp
    N1 Methyl Pseudoutp, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n1-methyl-pseudoutp/m1%CF%88+5++triphosphate/pmc10767207-193-28-29
    Average 90 stars, based on 1 article reviews
    n1-methyl-pseudoutp - by Bioz Stars, 2026-09
    90/100 stars

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    In Vitro:

    Article Title: Lipid nanoparticle-encapsulated DNA vaccine robustly induce superior immune responses to the mRNA vaccine in Syrian hamsters
    Article Snippet: A single in vitro transcription reaction of TS mRNA in 40 mL contained 1 mg pT7TS DNA as a template, 5 mM guanosine triphosphate, ATP, cytidine triphosphate, and N1-methyl-pseudoUTP (TriLink, San Diego), 4 mM CleanCap AG 30Ome, 0.002 U/mL pyrophosphatase (Thermo Fisher Scientific), 1 U RNase inhibitor, and 10 U/mL T7 polymerase; the reaction was incubated for 90 min at 37 C. The TS mRNA was isolated from the in vitro transcription reaction by using a MegaClear RNA purification kit (Thermo Fisher Scientific) and resuspended in H2O for LNP preparation.

    Article Title: Lipid nanoparticle-encapsulated DNA vaccine robustly induce superior immune responses to the mRNA vaccine in Syrian hamsters
    Article Snippet: A single in vitro transcription reaction of TS mRNA in 40 μL contained 1 μg pT7TS DNA as a template, 5 mM guanosine triphosphate, ATP, cytidine triphosphate, and N1-methyl-pseudoUTP (TriLink, San Diego), 4 mM CleanCap AG 3′Ome, 0.002 U/μL pyrophosphatase (Thermo Fisher Scientific), 1 U RNase inhibitor, and 10 U/μL T7 polymerase; the reaction was incubated for 90 min at 37°C.

    Incubation:

    Article Title: Lipid nanoparticle-encapsulated DNA vaccine robustly induce superior immune responses to the mRNA vaccine in Syrian hamsters
    Article Snippet: A single in vitro transcription reaction of TS mRNA in 40 mL contained 1 mg pT7TS DNA as a template, 5 mM guanosine triphosphate, ATP, cytidine triphosphate, and N1-methyl-pseudoUTP (TriLink, San Diego), 4 mM CleanCap AG 30Ome, 0.002 U/mL pyrophosphatase (Thermo Fisher Scientific), 1 U RNase inhibitor, and 10 U/mL T7 polymerase; the reaction was incubated for 90 min at 37 C. The TS mRNA was isolated from the in vitro transcription reaction by using a MegaClear RNA purification kit (Thermo Fisher Scientific) and resuspended in H2O for LNP preparation.

    Article Title: Lipid nanoparticle-encapsulated DNA vaccine robustly induce superior immune responses to the mRNA vaccine in Syrian hamsters
    Article Snippet: A single in vitro transcription reaction of TS mRNA in 40 μL contained 1 μg pT7TS DNA as a template, 5 mM guanosine triphosphate, ATP, cytidine triphosphate, and N1-methyl-pseudoUTP (TriLink, San Diego), 4 mM CleanCap AG 3′Ome, 0.002 U/μL pyrophosphatase (Thermo Fisher Scientific), 1 U RNase inhibitor, and 10 U/μL T7 polymerase; the reaction was incubated for 90 min at 37°C.

    Isolation:

    Article Title: Lipid nanoparticle-encapsulated DNA vaccine robustly induce superior immune responses to the mRNA vaccine in Syrian hamsters
    Article Snippet: A single in vitro transcription reaction of TS mRNA in 40 mL contained 1 mg pT7TS DNA as a template, 5 mM guanosine triphosphate, ATP, cytidine triphosphate, and N1-methyl-pseudoUTP (TriLink, San Diego), 4 mM CleanCap AG 30Ome, 0.002 U/mL pyrophosphatase (Thermo Fisher Scientific), 1 U RNase inhibitor, and 10 U/mL T7 polymerase; the reaction was incubated for 90 min at 37 C. The TS mRNA was isolated from the in vitro transcription reaction by using a MegaClear RNA purification kit (Thermo Fisher Scientific) and resuspended in H2O for LNP preparation.

    Article Title: Lipid nanoparticle-encapsulated DNA vaccine robustly induce superior immune responses to the mRNA vaccine in Syrian hamsters
    Article Snippet: A single in vitro transcription reaction of TS mRNA in 40 μL contained 1 μg pT7TS DNA as a template, 5 mM guanosine triphosphate, ATP, cytidine triphosphate, and N1-methyl-pseudoUTP (TriLink, San Diego), 4 mM CleanCap AG 3′Ome, 0.002 U/μL pyrophosphatase (Thermo Fisher Scientific), 1 U RNase inhibitor, and 10 U/μL T7 polymerase; the reaction was incubated for 90 min at 37°C.

    Purification:

    Article Title: Lipid nanoparticle-encapsulated DNA vaccine robustly induce superior immune responses to the mRNA vaccine in Syrian hamsters
    Article Snippet: A single in vitro transcription reaction of TS mRNA in 40 mL contained 1 mg pT7TS DNA as a template, 5 mM guanosine triphosphate, ATP, cytidine triphosphate, and N1-methyl-pseudoUTP (TriLink, San Diego), 4 mM CleanCap AG 30Ome, 0.002 U/mL pyrophosphatase (Thermo Fisher Scientific), 1 U RNase inhibitor, and 10 U/mL T7 polymerase; the reaction was incubated for 90 min at 37 C. The TS mRNA was isolated from the in vitro transcription reaction by using a MegaClear RNA purification kit (Thermo Fisher Scientific) and resuspended in H2O for LNP preparation.

    Article Title: Lipid nanoparticle-encapsulated DNA vaccine robustly induce superior immune responses to the mRNA vaccine in Syrian hamsters
    Article Snippet: A single in vitro transcription reaction of TS mRNA in 40 μL contained 1 μg pT7TS DNA as a template, 5 mM guanosine triphosphate, ATP, cytidine triphosphate, and N1-methyl-pseudoUTP (TriLink, San Diego), 4 mM CleanCap AG 3′Ome, 0.002 U/μL pyrophosphatase (Thermo Fisher Scientific), 1 U RNase inhibitor, and 10 U/μL T7 polymerase; the reaction was incubated for 90 min at 37°C.



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