nti mlc2 (Proteintech)
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Nti Mlc2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mylpf/Myosin+Light+Chain+2%2FMLC-2V+Antibody/pm41933938-251-40-42
Average 94 stars, based on 205 article reviews
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Incubation:Article Title: Fast Myosin Binding Protein‐C Is a Vital Regulator in Young and Aged Fast Skeletal Muscle Homeostasis Article Snippet: About 10–20 μg of each sample lysate was loaded onto 4%–20% precast polyacrylamide gels (BioRad, Mini‐Protean TGX, Cat #4561094) and electrophoresed at 80–100 V for 90 min. .. Subsequently, the samples were transferred onto nitrocellulose membranes and blocked in 5% nonfat dry milk for 1 h. Primary antibodies, including sMyBP‐C (WH0004604M1 and SAB3501005, Sigma‐Aldrich), fMyBP‐C (SAB2108180, Sigma‐Aldrich), Myh4 (BF‐F3, DSHB), MyoM1 (20360‐1‐AP, Proteintech), Article Title: Fast Myosin Binding Protein-C is a Vital Regulator in Young and Aged Fast Skeletal Muscle Homeostasis Article Snippet: .. Primary antibodies, including sMyBP-C (WH0004604M1 and SAB3501005, Sigma-Aldrich), fMyBP-C (SAB2108180, Sigma-Aldrich), Myh4 (BF-F3, DSHB), MyoM1 (20360-1-AP, Proteintech), Article Title: Fast Myosin Binding Protein-C Is a Vital Regulator in Young and Aged Fast Skeletal Muscle Homeostasis. Article Snippet: About 10–20 μg of each sample lysate was loaded onto 4%–20% precast polyacrylamide gels (BioRad, MiniProtean TGX, Cat #4561094) and electrophoresed at 80–100 V for 90 min. .. Subsequently, the samples were transferred onto nitrocellulose membranes and blocked in 5% nonfat dry milk for 1 h. Primary antibodies, including sMyBP- C (WH0004604M1 and SAB3501005, Sigma- Aldrich), fMyBP- C (SAB2108180, Sigma- Aldrich), Myh4 (BF- F3, DSHB), MyoM1 (20360- 1- AP, Proteintech), Immunostaining:Article Title: Gene Transfer of Skeletal Muscle-Type Myosin Light Chain Kinase via Adeno-Associated Virus 6 Improves Muscle Functions in an Amyotrophic Lateral Sclerosis Mouse Model Article Snippet: .. Antibodies used were anti-FLAG antibodies (1:400 dilution for immunostaining, no. 14793; Cell Signaling Technology, Danvers, MA, USA); Alexa Fluor secondary antibodies (1:500 dilution for immunostaining, Thermo Fisher Scientific, Waltham, MA, USA); rhodamine phalloidin (3:500 dilution for immunostaining, PHDR1; Cytoskeleton, Inc., Denver, CO, USA); anti-FLAG ® M2-HRP antibody (1:4,000 dilution for immunoblotting, A8592; Sigma–Aldrich, St. Louis, MO, USA); anti-GFP HRP-DirecT antibody (1:2000 for immunoblotting, no. 598-7; MBL, Nagoya, Japan); anti-α-tubulin antibody (1:1000 for immunoblotting, no. B-5-1-2; Abcam, Cambridge, UK); HRP-coupled goat anti-mouse antibody (1:8,000, no. 55550; MP biomedicals, Santa Ana, CA, USA); and anti-skeletal RLC known as Western Blot:Article Title: Gene Transfer of Skeletal Muscle-Type Myosin Light Chain Kinase via Adeno-Associated Virus 6 Improves Muscle Functions in an Amyotrophic Lateral Sclerosis Mouse Model Article Snippet: .. Antibodies used were anti-FLAG antibodies (1:400 dilution for immunostaining, no. 14793; Cell Signaling Technology, Danvers, MA, USA); Alexa Fluor secondary antibodies (1:500 dilution for immunostaining, Thermo Fisher Scientific, Waltham, MA, USA); rhodamine phalloidin (3:500 dilution for immunostaining, PHDR1; Cytoskeleton, Inc., Denver, CO, USA); anti-FLAG ® M2-HRP antibody (1:4,000 dilution for immunoblotting, A8592; Sigma–Aldrich, St. Louis, MO, USA); anti-GFP HRP-DirecT antibody (1:2000 for immunoblotting, no. 598-7; MBL, Nagoya, Japan); anti-α-tubulin antibody (1:1000 for immunoblotting, no. B-5-1-2; Abcam, Cambridge, UK); HRP-coupled goat anti-mouse antibody (1:8,000, no. 55550; MP biomedicals, Santa Ana, CA, USA); and anti-skeletal RLC known as Immunopeptidomics:Article Title: MUNC, an Enhancer RNA Upstream from the MYOD Gene, Induces a Subgroup of Myogenic Transcripts in trans Independently of MyoD Article Snippet: Secondary-antibody incubation was carried out for 1 h after washing and at 1:4,000 dilution before washing and incubation with Millipore Immobilon horseradish peroxidase (HRP) substrate. .. Antibodies were used as follows: MyoD1 (sc-12732; Santa Cruz Co.), MHC (MF-20; Developmental Studies Hybridoma Bank, University of Iowa), Ablim3 (sc-398575; Santa Cruz Co.), |
