Review

mv1 (ATCC)

ATCC is a verified supplier

ATCC manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

ATCC mv1

Replication of bovine- and mink-derived H5N1 viruses in mammalian cell lines with and without raw milk supplementation. Viral replication levels expressed as log 10 TCID50 equivalents per mL from RT-qPCR targeting the influenza A matrix gene in MDCK, A549, MDBK, Vero, and <t>MV1</t> cells at 24 h post-infection (MOI = 0.01) with bovine H5N1 (A/dairy cattle/Kansas/5/2024) or mink H5N1 (A/Mink/Spain/3691-8_22VIR10586-10/2022). Cells were incubated in standard medium (without milk) or raw milk–milk-supplemented medium (with milk). Data represent individual technical replicates ( n = 3). Viral loads were calculated using a validated standard curve correlating Ct values to known viral titers (R 2 = 0.9779, linear range 10 2 –10 8 TCID50/mL). Results represent RNA levels as log 10 TCID50 equivalents; correlation with infectious virus production requires validation.

Mv1, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 511 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mv1/product/ATCC
Average 95 stars, based on 511 article reviews

mv1 - by Bioz Stars, 2026-05

95/100 stars

Images

1) Product Images from "Differential Host Responses and Viral Replication of Highly Pathogenic Avian Influenza H5N1 Strains in Diverse Cell Lines with a Raw Milk Supplement"

Article Title: Differential Host Responses and Viral Replication of Highly Pathogenic Avian Influenza H5N1 Strains in Diverse Cell Lines with a Raw Milk Supplement

Journal: Life

doi: 10.3390/life15101625

Figure Legend Snippet: Replication of bovine- and mink-derived H5N1 viruses in mammalian cell lines with and without raw milk supplementation. Viral replication levels expressed as log 10 TCID50 equivalents per mL from RT-qPCR targeting the influenza A matrix gene in MDCK, A549, MDBK, Vero, and MV1 cells at 24 h post-infection (MOI = 0.01) with bovine H5N1 (A/dairy cattle/Kansas/5/2024) or mink H5N1 (A/Mink/Spain/3691-8_22VIR10586-10/2022). Cells were incubated in standard medium (without milk) or raw milk–milk-supplemented medium (with milk). Data represent individual technical replicates ( n = 3). Viral loads were calculated using a validated standard curve correlating Ct values to known viral titers (R 2 = 0.9779, linear range 10 2 –10 8 TCID50/mL). Results represent RNA levels as log 10 TCID50 equivalents; correlation with infectious virus production requires validation.

Techniques Used: Derivative Assay, Quantitative RT-PCR, Infection, Incubation, Virus, Biomarker Discovery

Host-and condition-specific amino acid substitutions in bovine- and mink-derived H5N1 viruses. Heatmaps of substitutions (≥5% frequency) in viral populations recovered 24 h post-infection from MDCK, A549, MDBK, Vero, and MV1 cells under standard (−milk) or raw milk-supplemented (+milk) conditions. ( A ) Bovine-H5N1 (A/dairy cattle/Kansas/5/2024). ( B ) Mink-H5N1 (A/Mink/Spain/3691-8_22VIR10586-10/2022). Purple boxes indicate the presence of the substitution.

Figure Legend Snippet: Host-and condition-specific amino acid substitutions in bovine- and mink-derived H5N1 viruses. Heatmaps of substitutions (≥5% frequency) in viral populations recovered 24 h post-infection from MDCK, A549, MDBK, Vero, and MV1 cells under standard (−milk) or raw milk-supplemented (+milk) conditions. ( A ) Bovine-H5N1 (A/dairy cattle/Kansas/5/2024). ( B ) Mink-H5N1 (A/Mink/Spain/3691-8_22VIR10586-10/2022). Purple boxes indicate the presence of the substitution.

Techniques Used: Derivative Assay, Infection

Transcriptional profiling of cell type- and environment-dependent responses to H5N1 infection. ( A ) Principal component analysis (PCA) of variance Stabilizing Transformation (VST)-normalized RNA-seq counts from A549, MDCK, MDBK, MV1, and Vero cells at 24 h post-infection with bovine-H5N1, mink-H5N1, or mock, with or without raw milk supplementation. Shapes denote infection type (bovine-H5N1 = circle; mink-H5N1 = triangle; mock = square) and colors indicate the cell lines with or without milk. ( B ) Gene Ontology (GO) enrichment for genes upregulated in bovine-H5N1 (gray) and mink-H5N1 (dark blue) infections. Points are sized by enrichment score and ordered by −log 10 ( p -value). Enrichment was computed in Metascape and visualized in R (ggplot2).

Figure Legend Snippet: Transcriptional profiling of cell type- and environment-dependent responses to H5N1 infection. ( A ) Principal component analysis (PCA) of variance Stabilizing Transformation (VST)-normalized RNA-seq counts from A549, MDCK, MDBK, MV1, and Vero cells at 24 h post-infection with bovine-H5N1, mink-H5N1, or mock, with or without raw milk supplementation. Shapes denote infection type (bovine-H5N1 = circle; mink-H5N1 = triangle; mock = square) and colors indicate the cell lines with or without milk. ( B ) Gene Ontology (GO) enrichment for genes upregulated in bovine-H5N1 (gray) and mink-H5N1 (dark blue) infections. Points are sized by enrichment score and ordered by −log 10 ( p -value). Enrichment was computed in Metascape and visualized in R (ggplot2).

Techniques Used: Infection, Transformation Assay, RNA Sequencing

Impact of H5N1 strain and milk supplementation on host genes involved in influenza A entry, adsorption, and uncoating. VST normalized RNA-seq counts for six entry/adsorption/uncoating-associated genes ( GNE, SLC35A1, ATP1B1, ATP1B4, ATP1A1, ATP1B3 ) were measured at 24 h post-infection in five mammalian cell lines (MDCK, MDBK, A549, MV1, and Vero). Cells were infected with either bovine-H5N1 or mink-H5N1 (MOI = 0.01) and incubated in infection medium with or without raw milk. Boxplots display the distribution of expression values ( n = 3 technical replicates): box = interquartile range (25th–75th percentiles), horizontal line = median, whiskers = 1.5 × IQR, and individual points = values beyond whiskers.

Figure Legend Snippet: Impact of H5N1 strain and milk supplementation on host genes involved in influenza A entry, adsorption, and uncoating. VST normalized RNA-seq counts for six entry/adsorption/uncoating-associated genes ( GNE, SLC35A1, ATP1B1, ATP1B4, ATP1A1, ATP1B3 ) were measured at 24 h post-infection in five mammalian cell lines (MDCK, MDBK, A549, MV1, and Vero). Cells were infected with either bovine-H5N1 or mink-H5N1 (MOI = 0.01) and incubated in infection medium with or without raw milk. Boxplots display the distribution of expression values ( n = 3 technical replicates): box = interquartile range (25th–75th percentiles), horizontal line = median, whiskers = 1.5 × IQR, and individual points = values beyond whiskers.

Techniques Used: Adsorption, RNA Sequencing, Infection, Incubation, Expressing

Impact of H5N1 strain and milk supplementation on host genes involved in influenza A genome replication, trafficking, and protein synthesis. VST normalized RNA-seq counts for genome replication, trafficking, and protein synthesis-associated genes ( ANP32B, EIF4A2, NUP98, NXF1, NUP205, KPNB1 ) measured at 24 h post-infection in five mammalian cell lines (MDCK, MDBK, A549, MV1, and Vero). Cells were infected with either bovine-H5N1 or mink-H5N1 (MOI = 0.01) and incubated in infection medium with or without raw milk. Boxplots display the distribution of expression values ( n = 3 technical replicates): box = interquartile range (25th–75th percentiles), horizontal line = median, whiskers = 1.5 × IQR, and individual points = values beyond whiskers.

Figure Legend Snippet: Impact of H5N1 strain and milk supplementation on host genes involved in influenza A genome replication, trafficking, and protein synthesis. VST normalized RNA-seq counts for genome replication, trafficking, and protein synthesis-associated genes ( ANP32B, EIF4A2, NUP98, NXF1, NUP205, KPNB1 ) measured at 24 h post-infection in five mammalian cell lines (MDCK, MDBK, A549, MV1, and Vero). Cells were infected with either bovine-H5N1 or mink-H5N1 (MOI = 0.01) and incubated in infection medium with or without raw milk. Boxplots display the distribution of expression values ( n = 3 technical replicates): box = interquartile range (25th–75th percentiles), horizontal line = median, whiskers = 1.5 × IQR, and individual points = values beyond whiskers.

Techniques Used: RNA Sequencing, Infection, Incubation, Expressing

Impact of H5N1strain and milk supplementation on host genes involved in host antiviral responses against influenza A virus. VST normalized RNA-seq counts for host antiviral responses-associated genes ( ISG15, RSAD2, MOV10, ZC3HAV1, SPOCK2, ISG20 ) measured at 24 h post-infection in five mammalian cell lines (MDCK, MDBK, A549, MV1, and Vero). Cells were infected with either bovine-H5N1 or mink-H5N1 (MOI = 0.01) and incubated in infection medium with or without raw milk. Boxplots display the distribution of expression values ( n = 3 technical replicates): box = interquartile range (25th–75th percentiles), horizontal line = median, whiskers = 1.5 × IQR, and individual points = values beyond whiskers.

Figure Legend Snippet: Impact of H5N1strain and milk supplementation on host genes involved in host antiviral responses against influenza A virus. VST normalized RNA-seq counts for host antiviral responses-associated genes ( ISG15, RSAD2, MOV10, ZC3HAV1, SPOCK2, ISG20 ) measured at 24 h post-infection in five mammalian cell lines (MDCK, MDBK, A549, MV1, and Vero). Cells were infected with either bovine-H5N1 or mink-H5N1 (MOI = 0.01) and incubated in infection medium with or without raw milk. Boxplots display the distribution of expression values ( n = 3 technical replicates): box = interquartile range (25th–75th percentiles), horizontal line = median, whiskers = 1.5 × IQR, and individual points = values beyond whiskers.

Techniques Used: Virus, RNA Sequencing, Infection, Incubation, Expressing

Figure Legend Snippet: Impact of H5N1 strain and milk supplementation on host genes involved in host antiviral responses against influenza A virus. VST normalized RNA-seq counts for host antiviral responses-associated genes ( TRIM14, TRIM21, TRIM25, TRIM35, OAS2, OASL ) measured at 24 h post-infection in five mammalian cell lines (MDCK, MDBK, A549, MV1, and Vero). Cells were infected with either bovine-H5N1 or mink-H5N1 (MOI = 0.01) and incubated in infection medium with or without raw milk. Boxplots display the distribution of expression values ( n = 3 technical replicates): box = interquartile range (25th–75th percentiles), horizontal line = median, whiskers = 1.5 × IQR, and individual points = values beyond whiskers.

Techniques Used: Virus, RNA Sequencing, Infection, Incubation, Expressing

Impact of H5N1 strain and milk supplementation on host genes involved in host signaling. VST normalized RNA-seq counts for genes associated with host signaling ( CLK1, PLK3, FGFR2, MAP2K3, TNK2, DCLK2 ) measured at 24 h post-infection in five mammalian cell lines (MDCK, MDBK, A549, MV1, and Vero). Cells were infected with either bovine-H5N1 or mink-H5N1 (MOI = 0.01) and incubated in infection medium with or without raw milk. Boxplots display the distribution of expression values ( n = 3 technical replicates): box = interquartile range (25th–75th percentiles), horizontal line = median, whiskers = 1.5 × IQR, and individual points = values beyond whiskers.

Figure Legend Snippet: Impact of H5N1 strain and milk supplementation on host genes involved in host signaling. VST normalized RNA-seq counts for genes associated with host signaling ( CLK1, PLK3, FGFR2, MAP2K3, TNK2, DCLK2 ) measured at 24 h post-infection in five mammalian cell lines (MDCK, MDBK, A549, MV1, and Vero). Cells were infected with either bovine-H5N1 or mink-H5N1 (MOI = 0.01) and incubated in infection medium with or without raw milk. Boxplots display the distribution of expression values ( n = 3 technical replicates): box = interquartile range (25th–75th percentiles), horizontal line = median, whiskers = 1.5 × IQR, and individual points = values beyond whiskers.

Techniques Used: RNA Sequencing, Infection, Incubation, Expressing

Impact of H5N1 strain and milk supplementation on host genes involved in virus assembly and trafficking. VST normalized RNA-seq counts for host genes associated with virus assembly and trafficking ( ARCN1, COPA, COPB1, COPB2, COPE, GBF1 ) measured at 24 h post-infection in five mammalian cell lines (MDCK, MDBK, A549, MV1, and Vero). Cells were infected with either bovine-H5N1 or mink-H5N1 (MOI = 0.01) and incubated in infection medium with or without raw milk. Boxplots display the distribution of expression values ( n = 3 technical replicates): box = interquartile range (25th–75th percentiles), horizontal line = median, whiskers = 1.5 × IQR, and individual points = values beyond whiskers.

Figure Legend Snippet: Impact of H5N1 strain and milk supplementation on host genes involved in virus assembly and trafficking. VST normalized RNA-seq counts for host genes associated with virus assembly and trafficking ( ARCN1, COPA, COPB1, COPB2, COPE, GBF1 ) measured at 24 h post-infection in five mammalian cell lines (MDCK, MDBK, A549, MV1, and Vero). Cells were infected with either bovine-H5N1 or mink-H5N1 (MOI = 0.01) and incubated in infection medium with or without raw milk. Boxplots display the distribution of expression values ( n = 3 technical replicates): box = interquartile range (25th–75th percentiles), horizontal line = median, whiskers = 1.5 × IQR, and individual points = values beyond whiskers.

Techniques Used: Virus, RNA Sequencing, Infection, Incubation, Expressing

Image Search Results

Journal: Life

Article Title: Differential Host Responses and Viral Replication of Highly Pathogenic Avian Influenza H5N1 Strains in Diverse Cell Lines with a Raw Milk Supplement

doi: 10.3390/life15101625

Figure Lengend Snippet: Replication of bovine- and mink-derived H5N1 viruses in mammalian cell lines with and without raw milk supplementation. Viral replication levels expressed as log 10 TCID50 equivalents per mL from RT-qPCR targeting the influenza A matrix gene in MDCK, A549, MDBK, Vero, and MV1 cells at 24 h post-infection (MOI = 0.01) with bovine H5N1 (A/dairy cattle/Kansas/5/2024) or mink H5N1 (A/Mink/Spain/3691-8_22VIR10586-10/2022). Cells were incubated in standard medium (without milk) or raw milk–milk-supplemented medium (with milk). Data represent individual technical replicates ( n = 3). Viral loads were calculated using a validated standard curve correlating Ct values to known viral titers (R 2 = 0.9779, linear range 10 2 –10 8 TCID50/mL). Results represent RNA levels as log 10 TCID50 equivalents; correlation with infectious virus production requires validation.

Article Snippet: Madin-Darby Canine Kidney (MDCK, ATCC, cat# CCL-34), Madin-Darby Bovine Kidney (MDBK, ATCC, cat# CCL-22), A549 (human lung carcinoma, ATCC, cat# CCL-185), MV1 (mink lung epithelial, ATCC, cat# CCL-64), and Vero (African green monkey kidney, ATCC, cat# CCL-81) cells were obtained from ATCC.

Techniques: Derivative Assay, Quantitative RT-PCR, Infection, Incubation, Virus, Biomarker Discovery

Journal: Life

Article Title: Differential Host Responses and Viral Replication of Highly Pathogenic Avian Influenza H5N1 Strains in Diverse Cell Lines with a Raw Milk Supplement

doi: 10.3390/life15101625

Figure Lengend Snippet: Host-and condition-specific amino acid substitutions in bovine- and mink-derived H5N1 viruses. Heatmaps of substitutions (≥5% frequency) in viral populations recovered 24 h post-infection from MDCK, A549, MDBK, Vero, and MV1 cells under standard (−milk) or raw milk-supplemented (+milk) conditions. ( A ) Bovine-H5N1 (A/dairy cattle/Kansas/5/2024). ( B ) Mink-H5N1 (A/Mink/Spain/3691-8_22VIR10586-10/2022). Purple boxes indicate the presence of the substitution.

Techniques: Derivative Assay, Infection

Journal: Life

Article Title: Differential Host Responses and Viral Replication of Highly Pathogenic Avian Influenza H5N1 Strains in Diverse Cell Lines with a Raw Milk Supplement

doi: 10.3390/life15101625

Figure Lengend Snippet: Transcriptional profiling of cell type- and environment-dependent responses to H5N1 infection. ( A ) Principal component analysis (PCA) of variance Stabilizing Transformation (VST)-normalized RNA-seq counts from A549, MDCK, MDBK, MV1, and Vero cells at 24 h post-infection with bovine-H5N1, mink-H5N1, or mock, with or without raw milk supplementation. Shapes denote infection type (bovine-H5N1 = circle; mink-H5N1 = triangle; mock = square) and colors indicate the cell lines with or without milk. ( B ) Gene Ontology (GO) enrichment for genes upregulated in bovine-H5N1 (gray) and mink-H5N1 (dark blue) infections. Points are sized by enrichment score and ordered by −log 10 ( p -value). Enrichment was computed in Metascape and visualized in R (ggplot2).

Techniques: Infection, Transformation Assay, RNA Sequencing

Journal: Life

Article Title: Differential Host Responses and Viral Replication of Highly Pathogenic Avian Influenza H5N1 Strains in Diverse Cell Lines with a Raw Milk Supplement

doi: 10.3390/life15101625

Figure Lengend Snippet: Impact of H5N1 strain and milk supplementation on host genes involved in influenza A entry, adsorption, and uncoating. VST normalized RNA-seq counts for six entry/adsorption/uncoating-associated genes ( GNE, SLC35A1, ATP1B1, ATP1B4, ATP1A1, ATP1B3 ) were measured at 24 h post-infection in five mammalian cell lines (MDCK, MDBK, A549, MV1, and Vero). Cells were infected with either bovine-H5N1 or mink-H5N1 (MOI = 0.01) and incubated in infection medium with or without raw milk. Boxplots display the distribution of expression values ( n = 3 technical replicates): box = interquartile range (25th–75th percentiles), horizontal line = median, whiskers = 1.5 × IQR, and individual points = values beyond whiskers.

Techniques: Adsorption, RNA Sequencing, Infection, Incubation, Expressing

Journal: Life

Article Title: Differential Host Responses and Viral Replication of Highly Pathogenic Avian Influenza H5N1 Strains in Diverse Cell Lines with a Raw Milk Supplement

doi: 10.3390/life15101625

Figure Lengend Snippet: Impact of H5N1 strain and milk supplementation on host genes involved in influenza A genome replication, trafficking, and protein synthesis. VST normalized RNA-seq counts for genome replication, trafficking, and protein synthesis-associated genes ( ANP32B, EIF4A2, NUP98, NXF1, NUP205, KPNB1 ) measured at 24 h post-infection in five mammalian cell lines (MDCK, MDBK, A549, MV1, and Vero). Cells were infected with either bovine-H5N1 or mink-H5N1 (MOI = 0.01) and incubated in infection medium with or without raw milk. Boxplots display the distribution of expression values ( n = 3 technical replicates): box = interquartile range (25th–75th percentiles), horizontal line = median, whiskers = 1.5 × IQR, and individual points = values beyond whiskers.

Techniques: RNA Sequencing, Infection, Incubation, Expressing

Journal: Life

Article Title: Differential Host Responses and Viral Replication of Highly Pathogenic Avian Influenza H5N1 Strains in Diverse Cell Lines with a Raw Milk Supplement

doi: 10.3390/life15101625

Figure Lengend Snippet: Impact of H5N1strain and milk supplementation on host genes involved in host antiviral responses against influenza A virus. VST normalized RNA-seq counts for host antiviral responses-associated genes ( ISG15, RSAD2, MOV10, ZC3HAV1, SPOCK2, ISG20 ) measured at 24 h post-infection in five mammalian cell lines (MDCK, MDBK, A549, MV1, and Vero). Cells were infected with either bovine-H5N1 or mink-H5N1 (MOI = 0.01) and incubated in infection medium with or without raw milk. Boxplots display the distribution of expression values ( n = 3 technical replicates): box = interquartile range (25th–75th percentiles), horizontal line = median, whiskers = 1.5 × IQR, and individual points = values beyond whiskers.

Techniques: Virus, RNA Sequencing, Infection, Incubation, Expressing

Journal: Life

Article Title: Differential Host Responses and Viral Replication of Highly Pathogenic Avian Influenza H5N1 Strains in Diverse Cell Lines with a Raw Milk Supplement

doi: 10.3390/life15101625

Figure Lengend Snippet: Impact of H5N1 strain and milk supplementation on host genes involved in host antiviral responses against influenza A virus. VST normalized RNA-seq counts for host antiviral responses-associated genes ( TRIM14, TRIM21, TRIM25, TRIM35, OAS2, OASL ) measured at 24 h post-infection in five mammalian cell lines (MDCK, MDBK, A549, MV1, and Vero). Cells were infected with either bovine-H5N1 or mink-H5N1 (MOI = 0.01) and incubated in infection medium with or without raw milk. Boxplots display the distribution of expression values ( n = 3 technical replicates): box = interquartile range (25th–75th percentiles), horizontal line = median, whiskers = 1.5 × IQR, and individual points = values beyond whiskers.

Techniques: Virus, RNA Sequencing, Infection, Incubation, Expressing

Journal: Life

Article Title: Differential Host Responses and Viral Replication of Highly Pathogenic Avian Influenza H5N1 Strains in Diverse Cell Lines with a Raw Milk Supplement

doi: 10.3390/life15101625

Figure Lengend Snippet: Impact of H5N1 strain and milk supplementation on host genes involved in host signaling. VST normalized RNA-seq counts for genes associated with host signaling ( CLK1, PLK3, FGFR2, MAP2K3, TNK2, DCLK2 ) measured at 24 h post-infection in five mammalian cell lines (MDCK, MDBK, A549, MV1, and Vero). Cells were infected with either bovine-H5N1 or mink-H5N1 (MOI = 0.01) and incubated in infection medium with or without raw milk. Boxplots display the distribution of expression values ( n = 3 technical replicates): box = interquartile range (25th–75th percentiles), horizontal line = median, whiskers = 1.5 × IQR, and individual points = values beyond whiskers.

Techniques: RNA Sequencing, Infection, Incubation, Expressing

Journal: Life

Article Title: Differential Host Responses and Viral Replication of Highly Pathogenic Avian Influenza H5N1 Strains in Diverse Cell Lines with a Raw Milk Supplement

doi: 10.3390/life15101625

Figure Lengend Snippet: Impact of H5N1 strain and milk supplementation on host genes involved in virus assembly and trafficking. VST normalized RNA-seq counts for host genes associated with virus assembly and trafficking ( ARCN1, COPA, COPB1, COPB2, COPE, GBF1 ) measured at 24 h post-infection in five mammalian cell lines (MDCK, MDBK, A549, MV1, and Vero). Cells were infected with either bovine-H5N1 or mink-H5N1 (MOI = 0.01) and incubated in infection medium with or without raw milk. Boxplots display the distribution of expression values ( n = 3 technical replicates): box = interquartile range (25th–75th percentiles), horizontal line = median, whiskers = 1.5 × IQR, and individual points = values beyond whiskers.

Techniques: Virus, RNA Sequencing, Infection, Incubation, Expressing

Review

Structured Review

Images

1) Product Images from "Differential Host Responses and Viral Replication of Highly Pathogenic Avian Influenza H5N1 Strains in Diverse Cell Lines with a Raw Milk Supplement"

Similar Products

Image Search Results