mesenchymal stem cell basal medium  (ATCC)

Journal: Journal of Translational Medicine

Article Title: Horizontal mitochondrial transfer and mitochondrial transplantation in skin: protection against UVR-induced ROS damage and enhancement of cell proliferation and wound healing

doi: 10.1186/s12967-026-08801-y

Figure Lengend Snippet: Analysis of AMT to skin fibroblasts using different concentrations of mitochondria from MSCs and fibroblasts to protect against and reduce UVR-induced ROS levels. a . Representative images showing the transfer of mitochondria from isolated MTR-labeled Wharton’s jelly MSCs (WJ-MSCs) and fibroblasts to CTG-labeled fibroblasts at doses of 500 and 2,500 ng. b . Assessment of ROS production in fibroblasts (F) that received 500 and 2,500 ng of mitochondria isolated from WJ-MSCs (MSCM) or from fibroblasts (FM) prior to UVR exposure. c . Evaluation of ROS production in fibroblasts following AMT of 500 and 2,500 ng of WJ-MSC mitochondria (MSCM). Data for b and c were obtained from five independent replicates, each with five technical repetitions by condition. Normality was assessed using the D’Agostino & Pearson test, followed by non-parametric Mann-Whitney (** p < 0.01 and * p < 0.05) and Kruskal-Wallis tests (**** p < 0.0001) to identify statistically significant differences between conditions

Article Snippet: After mitochondria isolation from human MSCs (procedure details in the corresponding part of the methods section) they were prepared for SEM and analyzed by an VEGA 3 scanning electron microscope (Tescan) at 30 kV.

Techniques: Analysis, Isolation, Labeling, MANN-WHITNEY

Journal: Journal of Translational Medicine

Article Title: Horizontal mitochondrial transfer and mitochondrial transplantation in skin: protection against UVR-induced ROS damage and enhancement of cell proliferation and wound healing

doi: 10.1186/s12967-026-08801-y

Figure Lengend Snippet: Stimulation of fibroblast proliferation via xenogeneic and allogeneic AMT using mitochondria isolated from MSCs. We cultured 20,000 human (H) and mouse (M) fibroblasts in a medium supplemented with 1% FBS and 1% PS. Subsequently, these fibroblasts were treated with 500 or 2,500 ng of mitochondria extracted from MSCs (both from humans and mice). After a 72-hour incubation period, cell counts were performed, and the fold-change in experimental conditions was calculated by comparing them to the control group. a . AMT of H fibroblasts with mitochondria isolated from H MSCs. b . AMT of M fibroblasts with mitochondria isolated from M MSCs. c . AMT of H fibroblasts with mitochondria isolated from M MSCs. The data presented in this figure originate from three independent replicates or assays, each consisting of at least three repetitions by condition. To assess normality, a D’Agostino & Pearson test was conducted, followed by non-parametric analyses, including Mann-Whitney, aimed at identifying statistically significant differences among the conditions (* p < 0.05, ** p < 0.01, *** p < 0.001)

Article Snippet: After mitochondria isolation from human MSCs (procedure details in the corresponding part of the methods section) they were prepared for SEM and analyzed by an VEGA 3 scanning electron microscope (Tescan) at 30 kV.

Techniques: Isolation, Cell Culture, Incubation, Control, MANN-WHITNEY

Journal: Journal of Translational Medicine

Article Title: Horizontal mitochondrial transfer and mitochondrial transplantation in skin: protection against UVR-induced ROS damage and enhancement of cell proliferation and wound healing

doi: 10.1186/s12967-026-08801-y

Figure Lengend Snippet: Evaluation of regenerative potential via allogeneic MSC injection and mitochondrial infusion in mouse wounds. Various doses of isolated mitochondria (25, 50, and 100 ng) and MSCs (ranging from 2.5 to 10 × 10⁵ cells) were injected at the wound site, with two injections per side, each consisting of 25 µl. (A detailed description of the methodology is provided in the Methods section.) a . Schematic representation of the application of MSCs and mitochondria. b . Representative images showing the injection procedure in a mouse. c . Representative histological images (H&E and Trichrome staining) taken 48 h after wounding and treatment with vehicle, 25 ng mitochondria, or 250,000 MSCs. Similar results were observed with increasing concentrations of both treatments. d . The wound healing index (WHI) provides a score based on granulation tissue formation, inflammatory infiltrate, collagen organization, and the relative amounts of early and mature collagen. e . Histogram presenting WHI data from at least three independent biological replicates, each with three technical replicates. Normality was assessed using the D’Agostino & Pearson test, followed by non-parametric analyses with Mann-Whitney (**** p < 0.0001) and Kruskal-Wallis (** p < 0.01) tests to identify statistically significant differences between conditions

Article Snippet: After mitochondria isolation from human MSCs (procedure details in the corresponding part of the methods section) they were prepared for SEM and analyzed by an VEGA 3 scanning electron microscope (Tescan) at 30 kV.

Techniques: Injection, Isolation, Staining, Tissue, MANN-WHITNEY

Journal: Journal of Translational Medicine

Article Title: Horizontal mitochondrial transfer and mitochondrial transplantation in skin: protection against UVR-induced ROS damage and enhancement of cell proliferation and wound healing

doi: 10.1186/s12967-026-08801-y

Figure Lengend Snippet: Local delivery of mitochondria isolated from human WJ-MSCs enhances early repair responses in porcine cutaneous wounds. Mitochondria were isolated from human WJ-MSCs and administered locally around 1 cm porcine cutaneous wounds. Each wound received vehicle or a total dose of 25, 50, or 100 ng of mitochondria, distributed among four 25 µL injections placed as close as possible to the wound margins, with two injections administered on each side of the wound, for a total injection volume of 100 µL per wound. Three assays were indepndently performed with three repetitions by condition, a total of 45 pigs were used in the assays, with one wound analyzed per animal and each animal assigned to a single experimental condition. Tissue samples were collected 48 h after wounding and treatment for histological analysis. a . Schematic representation of mitochondrial isolation from human WJ MSCs and local administration into the tissue adjacent to the wound. b . Representative photographs showing wound delineation and the local injection procedure. c . WHI in untreated control (CTL), vehicle-treated, and mitochondria-treated wounds. Individual symbols represent independent animals, with one wound analyzed per pig, and bars show group means with error bars representing (SD±/SEM). Normality was assessed before statistical analysis. Overall differences among groups were evaluated using the Kruskal–Wallis test, and the indicated pairwise comparisons were evaluated using the Mann–Whitney U test, as described in the Methods. d . Schematic representation of the anatomical regions used for spatial quantification of Ki67 positivity. On each side of the wound, the wound zone comprised the 600-µm region extending outward from the wound tip and included the wound-edge and newly formed epidermal regions together with the underlying dermis. The extreme comprised the subsequent 300-µm region located distal to the wound zone and represented tissue farther from the injury. The central wound bed was analyzed as a separate dermal compartment. Epidermal Ki67 positivity was quantified in the extreme and wound-zone regions, whereas dermal Ki67 positivity was quantified in the extreme, wound-zone, and central wound-bed regions. Areas containing hair follicles, sebaceous glands, or other skin appendages were excluded from the analysis. e . Quantification of Ki67 positivity in the epidermal and dermal compartments of CTL, vehicle-treated, and mitochondria-treated wounds. Ki67 positivity was calculated as the percentage of Ki67-positive nuclei relative to the total number of nuclei within each analyzed region. At least three non-overlapping histological areas were quantified per region and per wound, and the resulting measurements were averaged to generate a single animal-level value for statistical analysis. Box-and-whisker plots show the distribution of animal-level values across experimental conditions. For each anatomical region, the indicated pairwise comparisons among treatment groups were performed using Mann–Whitney unpaired t test, as described in the Methods. f . Representative H&E, Masson’s trichrome, and Ki67-stained tissue sections from CTL, vehicle-treated, and wounds treated with 25, 50, or 100 ng of mitochondrial protein. H&E staining illustrates overall tissue architecture and inflammatory-cell infiltration, Masson’s trichrome staining shows collagen deposition and organization, and Ki67 immunostaining identifies proliferating cells. Scale bars: d, 100 μm; f, 100 μm. ns, not significant; * P < 0.05; ** P < 0.01; **** P < 0.0001

Article Snippet: After mitochondria isolation from human MSCs (procedure details in the corresponding part of the methods section) they were prepared for SEM and analyzed by an VEGA 3 scanning electron microscope (Tescan) at 30 kV.

Techniques: Isolation, Injection, Tissue, Analysis, Control, MANN-WHITNEY, Whisker Assay, Staining, Immunostaining