Journal: Biology

Article Title: Grape Pomace Polyphenolic Extract Promotes Osteogenic Differentiation in Human Mesenchymal Stem Cells Through Activation of RUNX2 and NRF2 Transcription Factors: A Potential Natural Strategy for Osteoporosis Prevention

doi: 10.3390/biology15090719

Figure Lengend Snippet: Effects of GPE on human mesenchymal stem viability. BMSC and AdMSC were exposed at increasing concentrations of GPE (1, 5, 10 and 25 µg GAE/mL) for 24 h and cell viability was evaluated by MTT assay. Data are shown as mean ± SD ( n = 5); * p < 0.05 versus untreated control cells (CTR).

Article Snippet: Human bone marrow-derived mesenchymal stem cells (BMSCs) from healthy donors (ATCC-PCS-500-012) were purchased from ATCC (Milan, Italy) as well as mesenchymal stem cell basal medium (ATCC PCS500030) and mesenchymal stem cell growth kit for BMSC (ATCC PCS500041).

Techniques: MTT Assay, Control

Journal: bioRxiv

Article Title: Scalable longitudinal imaging and transcriptomics of cells in dynamic enclosures

doi: 10.64898/2026.05.05.723030

Figure Lengend Snippet: A: UMAP based on transcriptomic data from primary human preadipocytes differentiated for seven days on a fibronectin-coated flow cell. The colors correspond to different clusters based on transcriptomic analysis. B: Transcriptomic UMAP colored by the lipid accumulation score, defined as the ratio between the BODIPY stain and the nuclear stain in each CCE. The insets show examples of cells that are very close in gene expression space but differ in their lipid content. C: violin plots depicting the distribution of lipid accumulation scores (y axis) across the transcriptomic clusters (x axis). D: actual (x axis) vs predicted (y axis) lipid accumulation scores from the elastic net model. The plot is for the held-out test set (20% of the total data). E: Euler diagram showing the overlap between top-20 differentially expressed genes between transcriptomic clusters (blue) and model-selected predictors of lipid accumulation (pink). F: average Log2 fold-change between clusters (x axis) vs absolute model coefficient (y axis) for the genes selected by the model. The red color indicates genes that are among the top-20 differentially expressed genes between transcriptomic clusters. G: Gene expression UMAP colored by the top-3 positive predictors identified by the model, showing that the expression values of these genes are uniformly distributed across the UMAP based on global transcriptomic differences. H: UMAP based on transcriptomic data for BV2 mouse microglial cells. The colors correspond to different clusters based on transcriptomic analysis. I: transcriptomic UMAP colored by phagocytic activity as measured by pHrodo™ intensity after four hours. J: UMAP based on DINOv2 features, colored by phagocytic activity showing a greater degree of separation between high vs low phagocytic scores, compared to the transcriptomic UMAP in panel H. K: violin plots depicting the distribution of phagocytic scores (y axis) across the transcriptomic clusters (x axis). L: R 2 performance of elastic net models trained on expression-only features, DINOv2-only features or a combination of the two (x axis). The data refers to the held-out test set (20% of the total data). M: actual (x axis) vs predicted (y axis) phagocytic scores from the elastic net model using the combined expression and DINOv2 features. The plot is for the held-out test set (20% of the total data). N: Euler plot showing the overlap between top-20 differentially expressed genes between transcriptomic clusters (blue) and model-selected predictors of phagocytic activity (pink). O: average Log2 fold-change between clusters (x axis) vs absolute models coefficient (y axis) for the genes selected by the expression-only model. The red color indicates genes that are among the top-20 differentially expressed genes between transcriptomic clusters. P: ridge plots displaying the expression level (x axis) of Gpnmb and Clec4e across transcriptomic clusters (x axis). These two genes are among the top positive predictors for the gene expression-based model and have clear mechanistic evidence linking them to the phagocytosis process. However, their expression is very similar across all the transcriptomic clusters.

Article Snippet: Adipogenesis was induced using Adipocytes Differentiation Toolkit for Adipose Derived MSCs and Preadipocytes (ATCC, # PCS-500-050).

Techniques: Staining, Gene Expression, Expressing, Activity Assay

Journal: Experimental cell research

Article Title: Tumor stroma interaction is mediated by monocarboxylate metabolism

doi: 10.1016/j.yexcr.2017.01.013

Figure Lengend Snippet: Glycolytic flux and lactate metabolism. (A) The extracellular acidification rate (ECAR; mean±SD) of MDA-MB-231 cells and CAFs, measured by Seahorse analyzer, reveals that MDA-MB-231 cells are more glycolytic than CAFs. The extracellular acidification rate ECAR measures proton excretion (representing cellular glycolysis) over time in units mpH/min where 1 mpH = 4.3 pmole excreted H+. (B) Extracellular glucose consumption and lactate production of MDA-MB-231 cells and stromal cells confirm the higher glycolytic activity of MDA-MB-231 cells compared to CAFs observed by Seahorse Analyzer analysis. Data are displayed as mean±SD (n = 3). (C) The glucose uptake is significantly higher in MDA-MB-231 cells (MDAs) than in MSCs or CAFs, in good agreement with the higher aerobic glycolysis observed in the cancer cells. Data are displayed as mean±SD (n = 4). (D) CAFs take up and metabolize lactate as well as secrete lactate oxidation metabolites, as shown by 13C MR spectroscopy on cell extracts and CCM. Signal assignments are: α-KG – α-ketoglutarate, Glu – glutamate; Ala – alanine; Lac – lactate; Pyr – pyruvate; α-Glc & β-Glc – α-glucose and β-glucose; “-C” followed by number – position of 13C labeling as a result of metabolic conversion of exogenous 10 mM 3-13C-L-lactate. ** p<0.005, *** p<0.0005 by two-tailed, unpaired, unequal variance Student’s T-test; Abbreviations: MDAs – MDA-MB-231 cells; MSCs: human mesenchymal stem cells; CAFs: cancer-associated fibroblasts; CCM – CAF-conditioned medium;

Article Snippet: Bone marrow-derived mesenchymal stem cells (MSCs) were purchased from Lonza Walkersville, Inc. (Walkersville, MD; Lonza Group Ltd.).

Techniques: Activity Assay, Spectroscopy, Labeling, Two Tailed Test

Journal: Disease Models & Mechanisms

Article Title: Intramyocardial angiogenetic stem cells and epicardial erythropoietin save the acute ischemic heart

doi: 10.1242/dmm.033282

Figure Lengend Snippet: EPO induced synergistic angiogenesis with rat cardiac CD45 − CD44 + DDR2 + MSCs and facilitated cardiomyogenic differentiation. (A,B) Representative immunostaining images (A) from co-cultures of Qtracker ® -labeled (red) cardiac CD45 − CD44 + DDR2 + MSCs and cardiomyocytes (CM). MSCs (arrows) show partially positive expression for cardiomyogenic transcription factors GATA4 (yellow) and Nkx2.5 (green). Quantified GATA4 and Nkx2.5 signal intensities (B) illustrated promotion of cardiomyogenic differentiation in co-cultured MSCs compared with mono-cultured MSCs and strongest Nkx2.5 signal expression in co-cultured MSCs by continuous EPO stimulation. The average signal intensities from MSCs in mono-culture were arbitrarily given a value of 1 (2°). * P <0.05 versus cardiac CD45 − CD44 + DDR2 + MSC mono-culture. # P <0.05. Scale bars: 10 µm. Blue, DAPI in nuclei. (C-E) Representative phase-contrast microscopy images (C) from HUVECs in untreated mono-culture (C1, HUVEC control), in co-culture with rat cardiac CD45 − CD44 + DDR2 + MSCs (C2, HUVECs+MSCs), in MSC-conditioned medium (C3, Cond. DMEM-10), in EPO-supplemented medium (C4, DMEM-10+EPO) and in EPO-supplemented MSC-conditioned medium (C5, Cond. DMEM-10+EPO) with clear tubuli and network formations. Quantified HUVEC branching (D) and junctional network formation (E) illustrated synergistic angiogenetic potential of EPO and paracrine factors from cardiac CD45 − CD44 + DDR2 + MSCs. * P <0.05. Scale bars: 100 µm. Mean±s.e.m.

Article Snippet: Relative fluorescence signal intensities of intranuclear Nkx2.5 and GATA4 in Qtracker ® -labeled cardiac MSCs were quantified under standardized confocal laser scanning microscope settings (ELYRA PS.1 LSM 780, Carl Zeiss) and application of ZEN 2011 software (blue edition, Carl Zeiss).

Techniques: Immunostaining, Labeling, Expressing, Cell Culture, Microscopy, Co-Culture Assay

Journal: Molecular Therapy

Article Title: Spherical Bullet Formation via E-cadherin Promotes Therapeutic Potency of Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Myocardial Infarction

doi: 10.1038/mt.2012.58

Figure Lengend Snippet: Three dimensional (3D)-bullet formation increases vascular endothelial growth factor (VEGF) amount and S-phase fraction of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs). (a) Phase contrast microscopy showing the time course of 3D-bullet formation of hUCB-MSCs over 24 hours. (b) Propidium iodide (PI) fluorescence-activated cell sorter (FACS) of 3D-bullet hUCB-MSCs at 24 hours from anchorage deprivation. Increased S phase in 3D-bullets was observed. (c) Enzyme-linked immunosorbent assay (ELISA) measurement of human VEGF secretion at 1, 6, and 24 hours from anchorage deprivation in hUCB-MSCs.

Article Snippet: Medipost (Seoul, Korea) provided MSCs isolated from hUCB (UCB-MSCs) as previously described, 7 all these processes were carried out with informed consents.

Techniques: Derivative Assay, Microscopy, Fluorescence, Enzyme-linked Immunosorbent Assay

Journal: Molecular Therapy

Article Title: Spherical Bullet Formation via E-cadherin Promotes Therapeutic Potency of Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Myocardial Infarction

doi: 10.1038/mt.2012.58

Figure Lengend Snippet: Therapeutic efficacy of human umbilical cord blood-derived mesenchymal stem cell (hUCB-MSC) is enhanced by transplantation as three dimensional (3D)-bullet rather than as single cells in postinfarction left ventricular (LV) remodeling. (a) Time table for the transplantation of hUCB-MSCs in the rat myocardial infarction (MI) model. (b) Experimental scheme. (c) Left ventricular end-systolic diameter (LVESD) and delta LVESD, and left ventricular end-diastolic diameter (LVEDD) and delta LVEDD showed significant repression of remodeling in the 3D-bullet group (group “b”). (d) Left ventricular ejection fraction (LVEF) and delta LVEF, and left ventricular fractional shortening (LVFS) and delta LVFS showed significant improvement in systolic function in group “b” compared to the other groups. P < 0.05 versus control group. (e) Masson's trichome staining. (f) Efficacy in reducing infarct size. (g) Efficacy in preserving infarct wall thickness. Group “b” showed the highest efficacy in reducing infarct size and preserving infarct wall thickness compared to single cells from monolayer group “a.” n = 7 per group.

Article Snippet: Medipost (Seoul, Korea) provided MSCs isolated from hUCB (UCB-MSCs) as previously described, 7 all these processes were carried out with informed consents.

Techniques: Drug discovery, Derivative Assay, Transplantation Assay, Control, Staining, Preserving

Journal: Molecular Therapy

Article Title: Spherical Bullet Formation via E-cadherin Promotes Therapeutic Potency of Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Myocardial Infarction

doi: 10.1038/mt.2012.58

Figure Lengend Snippet: Engraftment of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) is improved after transplanted as three dimensional (3D)-bullets rather than as single cells. (a) The infracted heart at 3 weeks after cell transplantation was observed under fluorescence confocal microscopy. We used DiI-labeled hUCB-MSCs for tracking in vivo. DiI-positive cells were more frequently observed in group “b.” (b) Lectin-positive capillary density was higher in group “b” than “a” in peri-infarct tissue. Four weeks post- myocardial infarction (MI); n = 7 per group.

Article Snippet: Medipost (Seoul, Korea) provided MSCs isolated from hUCB (UCB-MSCs) as previously described, 7 all these processes were carried out with informed consents.

Techniques: Derivative Assay, Transplantation Assay, Fluorescence, Confocal Microscopy, Labeling, In Vivo

Journal: Molecular Therapy

Article Title: Spherical Bullet Formation via E-cadherin Promotes Therapeutic Potency of Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Myocardial Infarction

doi: 10.1038/mt.2012.58

Figure Lengend Snippet: Calcium-dependent adhesion molecule is involved in three dimensional (3D)-bullet formation. (a) Calcium-dependent cell–cell interaction was required for 3D-bullet formation in human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs). The calcium chelator EDTA inhibited 3D-bullet formation in hUCB-MSCs. (b) Screening of calcium-dependent cell adhesion molecules in a cDNA array between the 3D-bullet and monolayer. (c) Four genes (CDH1, CDH9, CDH16, SELE) showing a twofold or greater expression in the 3D-bullet were validated by real-time PCR. E-cadherin CDH1 was significantly more highly expressed in the 3D-bullet.

Article Snippet: Medipost (Seoul, Korea) provided MSCs isolated from hUCB (UCB-MSCs) as previously described, 7 all these processes were carried out with informed consents.

Techniques: Derivative Assay, Expressing, Real-time Polymerase Chain Reaction

Journal: Molecular Therapy

Article Title: Spherical Bullet Formation via E-cadherin Promotes Therapeutic Potency of Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Myocardial Infarction

doi: 10.1038/mt.2012.58

Figure Lengend Snippet: E-cadherin is necessary for three dimensional (3D)-bullet formation in human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs). (a) Phase contrast microscopy image of 3D-bullets in hanging drops. E-cadherin neutralization inhibited 3D-bullet formation. Monolayer cells were dissociated and induced to form 3D-bullet under E-cadherin blocking antibody (40 µg/ml; Sigma, U3254) or immunoglobulin G (IgG) for 6 hours. (b) mRNA of vascular endothelial growth factor (VEGF) was downregulated by E-cadherin neutralization. (c) Amount of VEGF secretion was reduced by E-cadherin neutralization, which was correlated with transcriptional regulation. (d) E-cadherin neutralization led to the inhibition of extracellular signal-regulated kinase (ERK) and AKT activation. (e) Quantification of ERK and AKT expression. The western blot was quantified using the software TINA 2.0 (Raytest, Straubenhardt, Germany). The hanging drop approach was used for this experiment.

Article Snippet: Medipost (Seoul, Korea) provided MSCs isolated from hUCB (UCB-MSCs) as previously described, 7 all these processes were carried out with informed consents.

Techniques: Derivative Assay, Microscopy, Neutralization, Blocking Assay, Inhibition, Activation Assay, Expressing, Western Blot, Software

Journal: Molecular Therapy

Article Title: Spherical Bullet Formation via E-cadherin Promotes Therapeutic Potency of Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Myocardial Infarction

doi: 10.1038/mt.2012.58

Figure Lengend Snippet: E-cadherin-induced vascular endothelial growth factor (VEGF) secretion is dependent on extracellular signal-regulated kinase (ERK)/AKT activation in three dimensional (3D)-bullet human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs). (a) Specific inhibition of ERK and AKT activation under E-cadherin overexpression. DMSO, a solvent for inhibitors, was used as a control. LY294002 was used to inhibit AKT activation. U0126 was used to inhibit ERK activation. (b) Inhibition of ERK and AKT activation suppressed VEGF secretion. ns, not significant.

Article Snippet: Medipost (Seoul, Korea) provided MSCs isolated from hUCB (UCB-MSCs) as previously described, 7 all these processes were carried out with informed consents.

Techniques: Activation Assay, Derivative Assay, Inhibition, Over Expression, Solvent, Control

Journal: Journal of Translational Medicine

Article Title: Advancing stem cell therapy from bench to bedside: lessons from drug therapies

doi: 10.1186/s12967-014-0243-9

Figure Lengend Snippet: Strategies to be refined to expand the stem cell therapeutic procedure

Article Snippet: Osiris Therapeutics , Autologous and Allogeneic Mesenchymal stem cells (MSCs) , OvationOS (bone matrix designed for the filling of bony voids and to support bone repair and regeneration) , Bone damage , , [ ] .

Techniques: Cell Tracking Assay, Transplantation Assay

Journal: Journal of Translational Medicine

Article Title: Advancing stem cell therapy from bench to bedside: lessons from drug therapies

doi: 10.1186/s12967-014-0243-9

Figure Lengend Snippet: Stem cell therapy companies and their cell products in development

Article Snippet: Osiris Therapeutics , Autologous and Allogeneic Mesenchymal stem cells (MSCs) , OvationOS (bone matrix designed for the filling of bony voids and to support bone repair and regeneration) , Bone damage , , [ ] .

Techniques: Derivative Assay, Extraction, Concentration Assay, Produced, Isolation, Vaccines, Cell Culture, Transplantation Assay, Activity Assay