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Image Search Results
Journal: Molecular cell
Article Title: The FUS::DDIT3 fusion oncoprotein inhibits BAF complex targeting and activity in myxoid liposarcoma
doi: 10.1016/j.molcel.2022.03.019
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Microarray, Recombinant, Sample Purification, Isolation, Derivative Assay, shRNA, Construct, CRISPR, Plasmid Preparation, Software
Journal: Molecular cell
Article Title: The FUS::DDIT3 fusion oncoprotein inhibits BAF complex targeting and activity in myxoid liposarcoma
doi: 10.1016/j.molcel.2022.03.019
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Microarray, Recombinant, Sample Purification, Isolation, Derivative Assay, shRNA, Construct, CRISPR, Plasmid Preparation, Software
Journal: Experimental cell research
Article Title: Tumor stroma interaction is mediated by monocarboxylate metabolism
doi: 10.1016/j.yexcr.2017.01.013
Figure Lengend Snippet: Glycolytic flux and lactate metabolism. (A) The extracellular acidification rate (ECAR; mean±SD) of MDA-MB-231 cells and CAFs, measured by Seahorse analyzer, reveals that MDA-MB-231 cells are more glycolytic than CAFs. The extracellular acidification rate ECAR measures proton excretion (representing cellular glycolysis) over time in units mpH/min where 1 mpH = 4.3 pmole excreted H+. (B) Extracellular glucose consumption and lactate production of MDA-MB-231 cells and stromal cells confirm the higher glycolytic activity of MDA-MB-231 cells compared to CAFs observed by Seahorse Analyzer analysis. Data are displayed as mean±SD (n = 3). (C) The glucose uptake is significantly higher in MDA-MB-231 cells (MDAs) than in MSCs or CAFs, in good agreement with the higher aerobic glycolysis observed in the cancer cells. Data are displayed as mean±SD (n = 4). (D) CAFs take up and metabolize lactate as well as secrete lactate oxidation metabolites, as shown by 13C MR spectroscopy on cell extracts and CCM. Signal assignments are: α-KG – α-ketoglutarate, Glu – glutamate; Ala – alanine; Lac – lactate; Pyr – pyruvate; α-Glc & β-Glc – α-glucose and β-glucose; “-C” followed by number – position of 13C labeling as a result of metabolic conversion of exogenous 10 mM 3-13C-L-lactate. ** p<0.005, *** p<0.0005 by two-tailed, unpaired, unequal variance Student’s T-test; Abbreviations: MDAs – MDA-MB-231 cells; MSCs: human mesenchymal stem cells; CAFs: cancer-associated fibroblasts; CCM – CAF-conditioned medium;
Article Snippet: Bone marrow-derived
Techniques: Activity Assay, Spectroscopy, Labeling, Two Tailed Test