pathway inhibition  (MedChemExpress)

Journal: International Dental Journal

Article Title: Meis1 Negatively Regulates Epithelial-Mesenchymal Transition via Wnt/β-catenin Pathway in Oral Submucous Fibrosis

doi: 10.1016/j.identj.2025.109323

Figure Lengend Snippet: Meis1 regulated EMT through the Wnt/β-catenin pathway. ( A ) q-PCR analysis of Meis1 knockdown efficiency in HaCaT cells transfected with sh-Meis1 plasmids, ** P < .01. ( B ) Western blot analysis of Meis1 knockdown efficiency in HaCaT cells transfected with sh-Meis1 plasmids. ( C ) Western blot analysis of lentivirus-mediated Meis1 knockdown efficiency by shRNA in HaCaT cells. ( D ) Venn diagram showing overlapping differentially expressed genes (DEGs) between the TGF-β1-induced group and sh-Meis1-2 group (C1: the control group; C2: TGF-β1-induced group; C3: sh-Meis1-2 group). ( E ) Pathway enrichment analysis of overlapping DEGs between the TGF-β1-induced group and sh-Meis1-2 group. ( F-H ) q-PCR analysis of β-catenin, cyclin D1, and c-Myc mRNA expression in cells with Meis1 overexpression, * P < .05, ** P < .01. ( I ) Western blot analysis of β-catenin, c-Jun, c-Myc, and cyclin D1 protein expression in cells with Meis1 overexpression. ( J-L ) q-PCR analysis of β-catenin, cyclin D1, and c-Myc mRNA expression in cells with Meis1 knockdown, * P < .05, ** P < .01. ( M ) Western blot analysis of β-catenin, c-Jun, c-Myc, and cyclin D1 protein expression in cells with Meis1 knockdown. ( N ) Western blot analysis of β-catenin protein expression in cells with or without MSAB treatment. ( O ) Western blot analysis of α-SMA, Vimentin, E-cadherin, and N-cadherin protein expression in cells with or without MSAB treatment.

Article Snippet: MSAB was used to treat HaCaT cells by blocking the Wnt/β-catenin pathway (MCE, HY-120697).

Techniques: Knockdown, Transfection, Western Blot, shRNA, Control, Expressing, Over Expression

Journal: bioRxiv

Article Title: Wnt/β-catenin signaling restrains developmental beige adipocyte thermogenesis and its inhibition imprints long-term energy expenditure

doi: 10.64898/2026.03.23.713637

Figure Lengend Snippet: a Experimental strategy to suppress β-catenin in vitro by employing adipocytes differentiated from SVFs of 4-week-old wild-type mouse iWAT. b Microscopy images of adipocytes following treatment with vehicle (0.1% DMSO), MSAB, Rosiglitazone, or MSAB plus Rosiglitazone ( n = 3 biological replicates). Scale bar, 200 μm. Nile Red and DAPI were used to stain lipid droplets and nuclei, respectively ( n = 3 biological replicates). Scale bar, 20 μm. The right panel displays the statistical analysis of Nile Red-labeled lipid droplets number per cell ( n = 32 cells). c mRNA expression of thermogenic genes in ( b ) ( n = 3 biological replicates). d Immunoblotting for Wnt5a/Ca²⁺-AMPK-PPAR axis and thermogenic protein in ( b ) ( n = 3 biological replicates). e Experimental strategy to suppress β-catenin while inhibiting the Ca²⁺ pathway in vitro by employing adipocytes differentiated from the SVFs of 4-week-old wild-type mouse iWAT. f Microscopy images of Rosiglitazone-differentiated adipocytes treated with vehicle (0.1% DMSO), MSAB, or MSAB plus BAPTA-AM ( n = 3 biological replicates). Scale bar, 200 μm. Nile Red and DAPI were used to stain lipid droplets and nuclei, respectively ( n = 3 biological replicates). Scale bar, 20 μm. The right panel displays the statistical analysis of Nile Red-labeled lipid droplets number per cell ( n = 32 cells). g mRNA expression of thermogenic genes in ( f ) ( n = 3 biological replicates). h Immunoblotting for Wnt5a/Ca²⁺-AMPK-PPAR axis and thermogenic protein in ( f ) ( n = 3 biological replicates). i OCR plots and measured maximal respiration levels across the three treatment groups (Vehicle, MSAB, MSAB and BAPTA-AM) ( n = 3 cells). The levels of mRNA expression are normalized to that of 36B4 . Data are the mean ± s.e.m. Statistical analyses used were unpaired two-sided Student’s t -tests or two-way ANOVA followed by Tukey’s multiple-comparisons test or one-way ANOVA with Tukey’s correction for multiple comparisons.

Article Snippet: For inhibition and activation experiments, once full adipogenic differentiation was confirmed, cells were treated with each of the following molecules for 4 days: MSAB (S6901,Selleck, 10 uM), BAPTA-AM (S7534, Selleck, 5 uM), Box5 (P1216, Selleck, 100 uM), Wnt5a recombinant protein (HY-P704313, MCE, 100 ng/mL) with the exception of Rosiglitazone (S2556, Selleck, 2 uM), which was added on the first day of adipogenic induction in SVFs.

Techniques: In Vitro, Microscopy, Staining, Labeling, Expressing, Western Blot

Journal: bioRxiv

Article Title: Wnt/β-catenin signaling restrains developmental beige adipocyte thermogenesis and its inhibition imprints long-term energy expenditure

doi: 10.64898/2026.03.23.713637

Figure Lengend Snippet: a Experimental strategy to suppress Wnt5a/Ca²⁺ pathway in vitro by employing adipocytes differentiated from the SVFs of 4-week-old wild-type mouse iWAT. b Microscopy images of adipocytes following treatment with vehicle (0.1% DMSO), Box5, MSAB, or MSAB plus Box5 ( n = 3 biological replicates). Scale bar, 200 μm. Nile Red and DAPI were used to stain lipid droplets and nuclei, respectively ( n = 3 biological replicates). Scale bar, 20 μm. The right panel displays the statistical analysis of Nile Red-labeled lipid droplets number per cell ( n = 32 cells). c mRNA expression of thermogenic genes and downstream genes of Wnt/β-catenin signaling pathway in ( b ) ( n = 3 biological replicates). d Immunoblotting for Wnt5a/Ca²⁺-AMPK-PPAR axis and thermogenic protein in ( b ) ( n = 3 biological replicates). e Experimental strategy to supply exogenous Wnt5a recombinant protein in vitro by employing adipocytes differentiated from the SVFs of 4-week-old wild-type mouse iWAT. f Microscopy images of adipocytes following treatment with vehicle (0.1% DMSO), Wnt5a recombinant protein, MSAB, or MSAB plus Wnt5a recombinant protein ( n = 3 biological replicates). Scale bar, 200 μm. Nile Red and DAPI were used to stain lipid droplets and nuclei, respectively ( n = 3 biological replicates). Scale bar, 20 μm. The right panel displays the statistical analysis of Nile Red-labeled lipid droplets number per cell ( n = 32 cells). g mRNA expression of thermogenic genes and downstream genes of Wnt/β-catenin signaling pathway in ( f ) ( n = 3 biological replicates). h Immunoblotting for Wnt5a/Ca²⁺-AMPK-PPAR axis and thermogenic protein in ( f ) ( n = 3 biological replicates). i OCR plots, measured basal respiration and measured maximal respiration levels in cultured peri-weaning adipocytes across the six treatment groups (Vehicle, Wnt5a recombinant protein, MSAB, MSAB plus Wnt5a recombinant protein, Box5, MSAB plus Box5) ( n = 3 cells). The levels of mRNA expression are normalized to that of 36B4 . Data are the mean ± s.e.m. Statistical analyses used were two-way ANOVA followed by Tukey’s multiple-comparisons test or one-way ANOVA with Tukey’s correction for multiple comparisons.

Article Snippet: For inhibition and activation experiments, once full adipogenic differentiation was confirmed, cells were treated with each of the following molecules for 4 days: MSAB (S6901,Selleck, 10 uM), BAPTA-AM (S7534, Selleck, 5 uM), Box5 (P1216, Selleck, 100 uM), Wnt5a recombinant protein (HY-P704313, MCE, 100 ng/mL) with the exception of Rosiglitazone (S2556, Selleck, 2 uM), which was added on the first day of adipogenic induction in SVFs.

Techniques: In Vitro, Microscopy, Staining, Labeling, Expressing, Western Blot, Recombinant, Cell Culture

Journal: Molecular Medicine Reports

Article Title: Collagen III regulates the termination of liver regeneration by suppressing hepatocyte proliferation and promoting functional recovery

doi: 10.3892/mmr.2026.13799

Figure Lengend Snippet: Liver regeneration status after collagen III degradation during the termination phase of liver regeneration. (A) Body weight of mice. (B) Liver-to-body weight ratio of mice. (C) Dual IF staining of EdU and Ki-67. (D) Dual IF staining of EdU and collagen III. Scale bars, 100 and 10 µm. *P<0.05, **P<0.01 and ***P<0.001. ns, not significant; PHx, partial hepatectomy; IF, immunofluorescence; Coln0.1, low-dose collagenase III; Coln0.2, high-dose collagenase III; Col III, collagen III.

Article Snippet: For the high-dose collagenase + MSAB group (Coln0.2 + MSAB group), 5 mg/kg/day MSAB (cat. no. HY-120697; MedChemExpress) was dissolved in an ultrasonic ice bath and administered intraperitoneally 2 h prior to each collagenase injection.

Techniques: Staining, Immunofluorescence

Journal: Molecular Medicine Reports

Article Title: Collagen III regulates the termination of liver regeneration by suppressing hepatocyte proliferation and promoting functional recovery

doi: 10.3892/mmr.2026.13799

Figure Lengend Snippet: Inhibition of β-catenin partially rescues impaired regeneration termination and liver dysfunction induced by collagen III degradation. (A) Schematic diagram of the animal experimental protocol; created with BioGDP.com (agreement no. GDP2025X2AT2F) . (B) WB analysis of β-catenin after collagen III degradation. (C) Body weight and (D) liver-to-body weight ratio of mice following collagen III degradation and β-catenin inhibition. (E) Dual immunofluorescence staining of EdU and collagen III following collagen III degradation and β-catenin inhibition. Scale bars, 100 and 10 µm. (F) Serum liver function marker levels following collagen III degradation and β-catenin inhibition. (G) WB analysis of HNF4α following collagen III degradation and β-catenin inhibition. (H) WB analysis of cyclin D1 following collagen III degradation and β-catenin inhibition. *P<0.05, **P<0.01 and ***P<0.001. ns, not significant; WB, western blot; PHx, partial hepatectomy; MSAB, methyl-sulfonyl AB; Coln0.1, low-dose collagenase III; Coln0.2, high-dose collagenase III; Col III, collagen III; ALT, alanine aminotransferase; HNF4α, hepatocyte nuclear factor 4α.

Article Snippet: For the high-dose collagenase + MSAB group (Coln0.2 + MSAB group), 5 mg/kg/day MSAB (cat. no. HY-120697; MedChemExpress) was dissolved in an ultrasonic ice bath and administered intraperitoneally 2 h prior to each collagenase injection.

Techniques: Inhibition, Immunofluorescence, Staining, Marker, Western Blot