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mr203485  (OriGene)


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    OriGene mr203485
    Mr203485, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mr203485/Psmb5+(NM_011186)+Mouse+Tagged+ORF+Clone/pmc11896255__ACEL___24___e14492___s001-0-8-9
    Average 93 stars, based on 3 article reviews
    mr203485 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Genetic and pharmacologic proteasome augmentation ameliorates Alzheimer’s-like pathology in mouse and fly APP overexpression models
    Article Snippet: Cells were imaged with the Incucyte system (Sartorius), and images were analyzed with the manufacturer’s software for green fluorescent protein (GFP) fluorescence intensity normalized by cell confluence. .. A full-length mouse PSMB5 plasmid was used (MR203485, Origene). .. PSMB5 was excised to remove the Myc-DDK tag then cloned into the ShuttleNSE empty vector (50958, Addgene) adjacent to the NSE promoter.

    Article Title: Proteasome Augmentation Mitigates Age‐Related Cognitive Decline in Mice
    Article Snippet: .. NSE-PSMB5 A full-length mouse PSMB5 plasmid was used (MR203485, Origene). .. PSMB5 was excised to remove the Myc-DDK tag then cloned into the ShuttleNSE empty vector (50958, Addgene) adjacent to the NSE promoter.

    Article Title: Genetic and pharmacologic proteasome augmentation ameliorates Alzheimer's-like pathology in mouse and fly APP overexpression models.
    Article Snippet: Cells were imaged with the Incucyte system (Sartorius), and images were analyzed with the manufacturer’s software for green fluorescent protein (GFP) fluorescence intensity normalized by cell confluence. .. A full-length mouse PSMB5 plasmid was used (MR203485, Origene). .. PSMB5 was excised to remove the Myc-DDK tag then cloned into the ShuttleNSE empty vector (50958, Addgene) adjacent to the NSE promoter.



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    mr203485  (OriGene)
    93
    OriGene mr203485
    Mr203485, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mr203485/Psmb5+(NM_011186)+Mouse+Tagged+ORF+Clone/pmc11896255__ACEL___24___e14492___s001-0-8-9
    Average 93 stars, based on 1 article reviews
    mr203485 - by Bioz Stars, 2026-10
    93/100 stars
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    length mouse psmb5 plasmid  (OriGene)
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    OriGene length mouse psmb5 plasmid
    Fig. 2. <t>PSMB5</t> overexpression increases proteasome function in mice and delays AD-like cognitive deficits and mortality. (A) NSE-PSMB5 transgenic mouse model. (B) Immunoblot of PSMB5 normalized to -actin, brains dissected from 3-month-old mice. NTG, N = 6; PSMB5, N = 7. (C) Native PAGE gel of brains dissected from 3-month-old mice; proteasome content and assembly assessed by incubation with the MV151 fluorescent proteasome probe. This is a broad proteasome inhibitor bind- ing to active centers and producing fluorescence. Values adjusted to total protein based on Coomassie staining. Purified 20S and 26S proteasome used to identify bands. NTG, N = 6; PSMB5, N = 7. (D) Relative proteasome peptidase (chymotrypsin-like) activity, brains dissected from 3-month-old mice (N = 5 to 6). Replicate samples were incubated with the proteasome inhibitor bortezomib. (E and F) NSE-PSMB5 reduces AD-like cell death (WST-1 assay) in MC65 AD cell culture model transfected with NSE- PSMB5, 4 days after tetracycline (TET) withdrawal, N = 24 samples per group. (G) NSE-PSMB5 mice crossed with hAPP(J20) AD model. (H to K) NSE-PSMB5 reduces AD-like learning and memory deficits in Morris water maze, Y maze, and novel object recognition assay. All animals were 7- to 8-month-old littermates; NTG, N = 15; PSMB5, N = 11; hAPP, N = 14; hAPP;PSMB5, N = 9. (L) NSE-PSMB5 protects against early mortality in hAPP(J20) mice; NTG, N = 38; PSMB5, N = 37; hAPP, N = 55; hAPP;PSMB5, N = 39. Animals are littermates. Significance was based on Student’s t test in (B) and (C). Significance was based on two-way ANOVA, followed by Tukey or Bonferroni post hoc test in (D), (F), (H), (J), and (K). Significance was based on log-rank test; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 were used unless otherwise stated. N represents the number of animals or samples per group.
    Length Mouse Psmb5 Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mr203485/Psmb5+(NM_011186)+Mouse+Tagged+ORF+Clone/pm35675410-367-1-8
    Average 93 stars, based on 1 article reviews
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    psmb5 (nm_011186) mouse tagged orf clone  (OriGene)
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    Fig. 2. <t>PSMB5</t> overexpression increases proteasome function in mice and delays AD-like cognitive deficits and mortality. (A) NSE-PSMB5 transgenic mouse model. (B) Immunoblot of PSMB5 normalized to -actin, brains dissected from 3-month-old mice. NTG, N = 6; PSMB5, N = 7. (C) Native PAGE gel of brains dissected from 3-month-old mice; proteasome content and assembly assessed by incubation with the MV151 fluorescent proteasome probe. This is a broad proteasome inhibitor bind- ing to active centers and producing fluorescence. Values adjusted to total protein based on Coomassie staining. Purified 20S and 26S proteasome used to identify bands. NTG, N = 6; PSMB5, N = 7. (D) Relative proteasome peptidase (chymotrypsin-like) activity, brains dissected from 3-month-old mice (N = 5 to 6). Replicate samples were incubated with the proteasome inhibitor bortezomib. (E and F) NSE-PSMB5 reduces AD-like cell death (WST-1 assay) in MC65 AD cell culture model transfected with NSE- PSMB5, 4 days after tetracycline (TET) withdrawal, N = 24 samples per group. (G) NSE-PSMB5 mice crossed with hAPP(J20) AD model. (H to K) NSE-PSMB5 reduces AD-like learning and memory deficits in Morris water maze, Y maze, and novel object recognition assay. All animals were 7- to 8-month-old littermates; NTG, N = 15; PSMB5, N = 11; hAPP, N = 14; hAPP;PSMB5, N = 9. (L) NSE-PSMB5 protects against early mortality in hAPP(J20) mice; NTG, N = 38; PSMB5, N = 37; hAPP, N = 55; hAPP;PSMB5, N = 39. Animals are littermates. Significance was based on Student’s t test in (B) and (C). Significance was based on two-way ANOVA, followed by Tukey or Bonferroni post hoc test in (D), (F), (H), (J), and (K). Significance was based on log-rank test; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 were used unless otherwise stated. N represents the number of animals or samples per group.
    Psmb5 (Nm 011186) Mouse Tagged Orf Clone, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mr203485/Psmb5+(NM_011186)+Mouse+Tagged+ORF+Clone/origene___mr203485
    Average 93 stars, based on 1 article reviews
    psmb5 (nm_011186) mouse tagged orf clone - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

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    Fig. 2. PSMB5 overexpression increases proteasome function in mice and delays AD-like cognitive deficits and mortality. (A) NSE-PSMB5 transgenic mouse model. (B) Immunoblot of PSMB5 normalized to -actin, brains dissected from 3-month-old mice. NTG, N = 6; PSMB5, N = 7. (C) Native PAGE gel of brains dissected from 3-month-old mice; proteasome content and assembly assessed by incubation with the MV151 fluorescent proteasome probe. This is a broad proteasome inhibitor bind- ing to active centers and producing fluorescence. Values adjusted to total protein based on Coomassie staining. Purified 20S and 26S proteasome used to identify bands. NTG, N = 6; PSMB5, N = 7. (D) Relative proteasome peptidase (chymotrypsin-like) activity, brains dissected from 3-month-old mice (N = 5 to 6). Replicate samples were incubated with the proteasome inhibitor bortezomib. (E and F) NSE-PSMB5 reduces AD-like cell death (WST-1 assay) in MC65 AD cell culture model transfected with NSE- PSMB5, 4 days after tetracycline (TET) withdrawal, N = 24 samples per group. (G) NSE-PSMB5 mice crossed with hAPP(J20) AD model. (H to K) NSE-PSMB5 reduces AD-like learning and memory deficits in Morris water maze, Y maze, and novel object recognition assay. All animals were 7- to 8-month-old littermates; NTG, N = 15; PSMB5, N = 11; hAPP, N = 14; hAPP;PSMB5, N = 9. (L) NSE-PSMB5 protects against early mortality in hAPP(J20) mice; NTG, N = 38; PSMB5, N = 37; hAPP, N = 55; hAPP;PSMB5, N = 39. Animals are littermates. Significance was based on Student’s t test in (B) and (C). Significance was based on two-way ANOVA, followed by Tukey or Bonferroni post hoc test in (D), (F), (H), (J), and (K). Significance was based on log-rank test; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 were used unless otherwise stated. N represents the number of animals or samples per group.

    Journal: Science advances

    Article Title: Genetic and pharmacologic proteasome augmentation ameliorates Alzheimer's-like pathology in mouse and fly APP overexpression models.

    doi: 10.1126/sciadv.abk2252

    Figure Lengend Snippet: Fig. 2. PSMB5 overexpression increases proteasome function in mice and delays AD-like cognitive deficits and mortality. (A) NSE-PSMB5 transgenic mouse model. (B) Immunoblot of PSMB5 normalized to -actin, brains dissected from 3-month-old mice. NTG, N = 6; PSMB5, N = 7. (C) Native PAGE gel of brains dissected from 3-month-old mice; proteasome content and assembly assessed by incubation with the MV151 fluorescent proteasome probe. This is a broad proteasome inhibitor bind- ing to active centers and producing fluorescence. Values adjusted to total protein based on Coomassie staining. Purified 20S and 26S proteasome used to identify bands. NTG, N = 6; PSMB5, N = 7. (D) Relative proteasome peptidase (chymotrypsin-like) activity, brains dissected from 3-month-old mice (N = 5 to 6). Replicate samples were incubated with the proteasome inhibitor bortezomib. (E and F) NSE-PSMB5 reduces AD-like cell death (WST-1 assay) in MC65 AD cell culture model transfected with NSE- PSMB5, 4 days after tetracycline (TET) withdrawal, N = 24 samples per group. (G) NSE-PSMB5 mice crossed with hAPP(J20) AD model. (H to K) NSE-PSMB5 reduces AD-like learning and memory deficits in Morris water maze, Y maze, and novel object recognition assay. All animals were 7- to 8-month-old littermates; NTG, N = 15; PSMB5, N = 11; hAPP, N = 14; hAPP;PSMB5, N = 9. (L) NSE-PSMB5 protects against early mortality in hAPP(J20) mice; NTG, N = 38; PSMB5, N = 37; hAPP, N = 55; hAPP;PSMB5, N = 39. Animals are littermates. Significance was based on Student’s t test in (B) and (C). Significance was based on two-way ANOVA, followed by Tukey or Bonferroni post hoc test in (D), (F), (H), (J), and (K). Significance was based on log-rank test; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 were used unless otherwise stated. N represents the number of animals or samples per group.

    Article Snippet: A full-length mouse PSMB5 plasmid was used (MR203485, Origene).

    Techniques: Over Expression, Transgenic Assay, Western Blot, Clear Native PAGE, Incubation, Fluorescence, Staining, Purification, Activity Assay, WST-1 Assay, Cell Culture, Transfection

    Fig. 3. PSMB5 overexpression increases degradation of APP machinery. (A and B) Immunoblot of 70-day-old Elav-GS-GAL4>UAS-hAPP;UAS-hBACE1 and Elav-GS- GAL4>UAS-hAPP;UAS-hBACE1;UAS-Pros5 flies fed with 400 M RU-486. AD model, N = 12; AD + Pros5, N = 12. Values normalized by -actin. (C and D) Immunoblot of APP levels in SK-N-SH cells transfected with either NSE vector (control) or NSE-PSMB5, N = 3. (E) Images of SK-N-SH cells transfected with APPGFP and either the NSE vector or NSE-PSMB5, N = 6. (F) NSE-PSMB5 mice crossed with hAPP(J20) AD model. (G) Anti-APP immunoblots in 7- to 8-month-old hAPP(J20) ± NSE-PSMB5 mouse brains. APP, N = 9; APP;PSMB5, N = 9. Values normalized by -actin with quantification (left) and A42 ELISA (right); APP, N = 9; APP;PSMB5, N = 9. (H) X34 staining on brain coronal sections from hAPP(J20) ± NSE-PSMB5 mice, N = 3 per genotype. Number of plaques per hemibrain were quantified using ImageJ software. (I) Postmortem human hippo- campal tissue (patients with AD versus age-matched unaffected controls). (J) Postmortem human hippocampal tissue sample lysate, proteasome activity plotted against soluble A ELISA or anti-APP immunoblot (N = 35 in total, 11 controls and 24 patients with AD). (K to O) Postmortem human hippocampal tissue sample lysate, protea- some activity plotted against phosphorylated tau/total tau ratio (L), BACE1 (M), K48-polyubiquitinated (K48-UB) proteins (N), and total polyubiquitinated (poly-UB) pro- teins (O). N = 23. *P < 0.05 and ***P < 0.001. Significance is based on Student’s t test in (B), (E), (G), and (H). Significance was based on linear regression in (J) to (O). N represents the number of animals, samples, or patients per group.

    Journal: Science advances

    Article Title: Genetic and pharmacologic proteasome augmentation ameliorates Alzheimer's-like pathology in mouse and fly APP overexpression models.

    doi: 10.1126/sciadv.abk2252

    Figure Lengend Snippet: Fig. 3. PSMB5 overexpression increases degradation of APP machinery. (A and B) Immunoblot of 70-day-old Elav-GS-GAL4>UAS-hAPP;UAS-hBACE1 and Elav-GS- GAL4>UAS-hAPP;UAS-hBACE1;UAS-Pros5 flies fed with 400 M RU-486. AD model, N = 12; AD + Pros5, N = 12. Values normalized by -actin. (C and D) Immunoblot of APP levels in SK-N-SH cells transfected with either NSE vector (control) or NSE-PSMB5, N = 3. (E) Images of SK-N-SH cells transfected with APPGFP and either the NSE vector or NSE-PSMB5, N = 6. (F) NSE-PSMB5 mice crossed with hAPP(J20) AD model. (G) Anti-APP immunoblots in 7- to 8-month-old hAPP(J20) ± NSE-PSMB5 mouse brains. APP, N = 9; APP;PSMB5, N = 9. Values normalized by -actin with quantification (left) and A42 ELISA (right); APP, N = 9; APP;PSMB5, N = 9. (H) X34 staining on brain coronal sections from hAPP(J20) ± NSE-PSMB5 mice, N = 3 per genotype. Number of plaques per hemibrain were quantified using ImageJ software. (I) Postmortem human hippo- campal tissue (patients with AD versus age-matched unaffected controls). (J) Postmortem human hippocampal tissue sample lysate, proteasome activity plotted against soluble A ELISA or anti-APP immunoblot (N = 35 in total, 11 controls and 24 patients with AD). (K to O) Postmortem human hippocampal tissue sample lysate, protea- some activity plotted against phosphorylated tau/total tau ratio (L), BACE1 (M), K48-polyubiquitinated (K48-UB) proteins (N), and total polyubiquitinated (poly-UB) pro- teins (O). N = 23. *P < 0.05 and ***P < 0.001. Significance is based on Student’s t test in (B), (E), (G), and (H). Significance was based on linear regression in (J) to (O). N represents the number of animals, samples, or patients per group.

    Article Snippet: A full-length mouse PSMB5 plasmid was used (MR203485, Origene).

    Techniques: Over Expression, Western Blot, Transfection, Plasmid Preparation, Control, Enzyme-linked Immunosorbent Assay, Staining, Software, Activity Assay