mpc11  (ATCC)

Journal: bioRxiv

Article Title: Metabolic silencing via methionine-based amino acid restriction in multiple myeloma cell lines reveals a potential new strategy for cancer therapy

doi: 10.1101/2025.08.17.670755

Figure Lengend Snippet: The cell lines MPC11, L363 and KMS12-BM were seeded at 10,000 cells/well in a 96-well plate. The cells were stimulated with control medium or Met(-)- medium. For the positive control (death), the cells were stimulated with 1 µM staurosporine. After 24 h, 48 h and 72 h, measurements were performed with the EarlyTox Live/Dead Assay Kit (Molecular Devices). The staining solution (5 µg/mL Hoechst 33342 (Thermo Fisher, Darmstadt, Germany), 6 µM EthD-III, and 6 µM calcein AM (CAM) in DMEM (Gibco, Life Technologies; Darmstadt, Germany)) was added, and after 30 min of incubation, measurements were made with the ImageXpress Pico Automated Cell Imaging System. The number of cells was determined via Hoechst 33342. Living cells were stained with the nonfluorescent calcein AM, which permeates the intact cell membrane and is converted into calcein, the fluorescent form, by intracellular esterases (cells are shown in green). If compromised cell membrane integrity is associated with cell death, EthD-III enters cells and binds to nucleic acids (cells are shown in red). A representative area for cell viability is shown for each cell line. The cell numbers are not representative of every case. The white bar, representing 52.44 µm, is used for size classification.

Article Snippet: The MPC11 murine MM cell line was purchased from the American Type Culture Collection (ATCC), and the KMS12-BM and L363 human MM cell lines were purchased from the Leibniz Institute, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).

Techniques: Control, Positive Control, Live Dead Assay, Staining, Incubation, Imaging, Membrane

Journal: Life Science Alliance

Article Title: APR-246 increases tumor antigenicity independent of p53

doi: 10.26508/lsa.202301999

Figure Lengend Snippet: Mice were immunized according to the schematic in . (A) Mean tumor size overtime of GVAX treatment in combination with anti-CTLA4 antibody (clone 9D9, BioXcell). An isotype antibody was used as a negative control (clone MPC11, BioXcell). (B) Survival of these mice, including post reimplantation with 0.1 million B16 cells at day 112. (C) Total number of tumor-free mice per treatment group (n = 30) when three independent experiments were pooled. (A, B, D, E, F, G, H, I) Individual mouse tumor volumes according to the treatment groups of the representative experiment from (A, B). The data represent mean ± SEM and the P -value is represented as *<0.0332, **<0.0021, ***<0.0002, ****<0.0001. P -value was calculated by two-way ANOVA for tumor growth curves, by log-rank (Mantel–Cox) test for survival curves and by unpaired t test for total number of mice comparison. n = 10, representative of three independent experiments.

Article Snippet: Therapeutic in vivo mAbs anti-CD40 (FGK45) and corresponding IgG isotype control (2A3), anti-CTLA-4 (clone 9D9) or the matched IgG isotype control (MPC11) were purchased from Bio X Cell.

Techniques: Negative Control, Comparison