Journal: Cell reports
Article Title: Clostridioides difficile toxin B subverts germinal center and antibody recall responses by stimulating a drug-treatable CXCR4-dependent mechanism
doi: 10.1016/j.celrep.2024.114245
Figure Lengend Snippet: Female mice ( n = 4 per group) were given 200 μL PBS vehicle control, 10 ng D270N, or 10 ng TcdB2 in PBS by the s.c. route. Seven days post treatment, RNA was purified from axillary and inguinal lymph nodes (aLNs and iLNs). Gene expression was quantified using the Nanostring nCounter SPRINT profiler platform. (A) Differentially expressed genes (DEGs) comparing TcdB2 to PBS (left), TcdB2 to D270N (center), or D270N to PBS (right). (B) Summary of the log2 fold change, raw p values, and adjusted p values (Benjamin-Yekutieli method) for each two-way comparison in the experiment. Values for cxcr4, cxcr5, ccr7 , and their ligands are depicted. A full list of chemokines and their receptors is shown in . (C) Relative expression of cxcr4, cxcr5 , and ccr7 in isolated B cells as determined by qPCR. Graphs show the increase in expression relative to vehicle-treated control mice and are normalized to gapdh expression using the ΔΔC T method. Data show mean ± SD for 5 mice per group. (D and E) B cell (D) and CD4 + T cell (E) CXCR4 and CXCR5 expression was measured by flow cytometry. Flow plots show CXCR4 versus CXCR5 expression, while graphs depict the mean ± SD percentage of each cell type expressing high levels of CXCR4. Data in are pooled from 3 experiments ( n = 9 per group). Statistical significance was determined by one-way ANOVA with Kruskal-Wallace post-test (** p < 0.01, *** p < 0.001).
Article Snippet: Gapdh Mouse qPCR Primer Pair , OriGene , Cat # MP205604.
Techniques: Control, Purification, Gene Expression, Comparison, Expressing, Isolation, Flow Cytometry
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