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recombinant mononucleosomes (EpiCypher)

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Structured Review

EpiCypher recombinant mononucleosomes
Recombinant Mononucleosomes, supplied by EpiCypher, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mononucleosomes/Mononucleosomes%2C+Recombinant/us12571051-654-45-59
Average 94 stars, based on 20 article reviews

recombinant mononucleosomes - by Bioz Stars, 2026-11

94/100 stars

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Mutagenesis:

Article Title: Different modes of engagement with the nucleosome acidic patch yield distinct functional outcomes
Article Snippet: .. All mononucleosomes ( EpiCypher ; Supplemental Table 1 ) were created from fully-defined (wild-type or mutant) octamers wrapped by 5’ biotinylated 147×601 DNA ( Supplemental Table 1) ( ; ). ..

Concentration Assay:

Article Title: Epigenetic memory is governed by an effector recruitment specificity toggle in Heterochromatin Protein 1
Article Snippet: .. Samples were prepared by varying concentrations of Swi6 while keeping substrate concentration, i.e. 10 nM mononucleosomes (H3K9me0 and H3K9me3, Epicypher catalog nos. ..

Article Title: Molecular determinants of antibody-mediated priming to enhance detection of ctDNA
Article Snippet: Tumor burden was monitored by luminescence using the In Vivo Imaging System (IVIS, PerkinElmer) every 3-5 days after tumor inoculation. .. Widom601 dsDNA, either free (cat. 16-0006, Epicypher) or histone bound in mononucleosomes (18-0005, Epicypher), was mixed to a final concentration of 0.2 ng/μL with each mAb binder to a concentration of 0.4 mg/mL in DPBS. ..

Article Title: Molecular determinants of antibody-mediated priming to enhance detection of ctDNA
Article Snippet: The gel was stained with 1:1000 SYBR Safe (cat. S33112, ThermoFisher) in 0.5X TBE for 30 minutes and imaged with an iBright imager (ThermoFisher). .. Streptavidin biosensor tips (cat. 18-5019, Sartorius) were used to immobilize mononucleosomes (16-0006, Epicypher), free dsDNA (18-0005, Epicypher) with the Widom601 sequence and a biotin tag, or biotinylated supercoiled or linearized pUC18 plasmid DNA, diluted to a final concentration of 8nM in kinetics buffer (5mg/ml BSA, 0.05% Tween-20 in PBS). .. Interaction with mAbs, diluted to various concentrations in filtered kinetics buffer, was tested at 37°C using Sartorius Octet R8 according to the following assay details: Baseline 150s, Loading 500s, Baseline2 100s, Association 400s, Dissociation 400s.

Recombinant:

Article Title: Thromboinflammation is associated with clinical outcome after ST-elevation myocardial infarction
Article Snippet: Optical density was measured at 650 nm using a multimode plate reader (FLUOstar omega; BMG Labtech). .. Different concentrations of recombinant mononucleosomes (H3Cit2,8,17; EpiCypher) diluted in standard diluent (50 mmol/L Tris-HCl pH 7.5; 300 mmol/L NaCl; 0.01 % [w/v] bovine serum albumin; and 0.01 % [v/v] Tween-20) served as calibration controls. ..

Article Title: Thromboinflammation is associated with clinical outcome after ST-elevation myocardial infarction.
Article Snippet: Optical density was measured at 650 nm using a multimode plate reader (FLUOstar omega; BMG Labtech). .. Different concentrations of recombinant mononucleosomes (H3Cit2,8,17; EpiCypher) diluted in standard diluent (50 mmol/L Tris-HCl pH 7.5; 300 mmol/L NaCl; 0.01 % [w/v] bovine serum albumin; and 0.01 % [v/v] Tween-20) served as calibration controls. ..

Binding Assay:

Article Title: Tracking live-cell single-molecule dynamics enables measurements of heterochromatin-associated protein–protein interactions
Article Snippet: After cleavage of 6× His-tag, the products were further isolated by anion exchange chromatography using a HiTRAP Q HP column (Cytiva). .. Reaction samples were prepared with varying concentrations of Chp2 and 50 nM mononucleosomes (Epicypher, H3K9, and H3K9me3) in binding buffer (20 mM HEPES pH 7.5, 4 mM Tris, 80 mM KCl, 0.1% Igepal CA-630, 0.2 mM EDTA, 2 mM DTT and 10% glycerol). .. Reaction samples were prepared with varying concentrations of Chp2 and 50 nM mononucleosomes (Epicypher, H3K9, and H3K9me3) in binding buffer (20 mM HEPES pH 7.5, 4 mM Tris, 80 mM KCl, 0.1% Igepal CA-630, 0.2 mM EDTA, 2 mM DTT and 10% glycerol).

Sequencing:

Plasmid Preparation:

Image Search Results

Journal: bioRxiv

Article Title: Tandem bromodomains of BRD4 cooperatively read poly-acetylated nucleosomes to enhance chromatin engagement and regulate breast cancer phenotypes

doi: 10.64898/2026.04.22.719657

Figure Lengend Snippet:

Article Snippet: Luminex nucleosome panels consisted of the following 601 147 bp DNA-biotinylated nucleosomes (all from EpiCypher): unmodified (rNuc), 16-0006; H4K12 acetylated ([H4K12ac] 2 ), 16-0312; H4 poly-acetylated ([H4K5acK8acK12acK16ac] 2 ), 16-0313; H3 poly-acetylated ([H3K4acK9acK14acK18ac] 2 ), 16-0336; H4 N-terminal tail truncated ([H4ΔN15] 2 ), 16-0018; and trypsin-digested tailless, 16-0027.

Techniques: Binding Assay, Fluorescence

The BRD4 tandem bromodomains stably associate with H4 poly-acetylated nucleosomes in biolayer interferometry. ( A-D ) Biolayer interferometry binding assays of ( A ) BD1-2 with H4 poly-acetylated nucleosomes ([H4K5acK8acK12acK16ac] 2 ), ( B ) BD1-2 with unacetylated nucleosomes, ( C ) BD1 alone with H4 poly-acetylated nucleosomes, and ( D ) BD1 alone with unacetylated nucleosomes. Protein concentrations are indicated at the right of each plot. Note the difference in y-axis scale between ( A ) 1 to -0.5 nm and ( B-D ) 0.3 to -0.3 nm. Octet fitting of BD1-2 binding curves to H4 poly-acetylated nucleosomes (12.5, 6.25, and 3.12 μM) using a 2:1 heterogeneous ligand model produced R 2 values >0.98 (Fig. S2). Experiments were performed in triplicate.

Journal: bioRxiv

Article Title: Tandem bromodomains of BRD4 cooperatively read poly-acetylated nucleosomes to enhance chromatin engagement and regulate breast cancer phenotypes

doi: 10.64898/2026.04.22.719657

Figure Lengend Snippet: The BRD4 tandem bromodomains stably associate with H4 poly-acetylated nucleosomes in biolayer interferometry. ( A-D ) Biolayer interferometry binding assays of ( A ) BD1-2 with H4 poly-acetylated nucleosomes ([H4K5acK8acK12acK16ac] 2 ), ( B ) BD1-2 with unacetylated nucleosomes, ( C ) BD1 alone with H4 poly-acetylated nucleosomes, and ( D ) BD1 alone with unacetylated nucleosomes. Protein concentrations are indicated at the right of each plot. Note the difference in y-axis scale between ( A ) 1 to -0.5 nm and ( B-D ) 0.3 to -0.3 nm. Octet fitting of BD1-2 binding curves to H4 poly-acetylated nucleosomes (12.5, 6.25, and 3.12 μM) using a 2:1 heterogeneous ligand model produced R 2 values >0.98 (Fig. S2). Experiments were performed in triplicate.

Techniques: Stable Transfection, Binding Assay, Produced

AlphaFold model suggesting a plausible mode of BRD4-S engagement with an H4 K12 acetylated nucleosome ([H4K12ac] 2 ). ( A ) Top-ranked AlphaFold model prediction of BRD4-S (blue) in complex with an H4K12ac nucleosome (H2A, golden; H2B, tomato; H3, green; H4, violet, Widom 601 DNA, grey). BD1 and BD2 are shaded in darker blue for clarity. ( B ) Rotated (45°) view of the modeled BRD4-S-H4K12ac nucleosome complex. ( C ) Zoom-in of the BD1 acetyl-lysine binding pocket highlighting residues Y97 and N140 positioned near H4K12ac. ( D ) Zoom-in of the BD2 acetyl-lysine binding pocket highlighting residues Y390 and N433 positioned near H4K12ac. This view also shows the relevant placement of the C-terminal BRD4-S region including the BID, ET, and phosphoregulatory sites, in the model.

Journal: bioRxiv

Article Title: Tandem bromodomains of BRD4 cooperatively read poly-acetylated nucleosomes to enhance chromatin engagement and regulate breast cancer phenotypes

doi: 10.64898/2026.04.22.719657

Figure Lengend Snippet: AlphaFold model suggesting a plausible mode of BRD4-S engagement with an H4 K12 acetylated nucleosome ([H4K12ac] 2 ). ( A ) Top-ranked AlphaFold model prediction of BRD4-S (blue) in complex with an H4K12ac nucleosome (H2A, golden; H2B, tomato; H3, green; H4, violet, Widom 601 DNA, grey). BD1 and BD2 are shaded in darker blue for clarity. ( B ) Rotated (45°) view of the modeled BRD4-S-H4K12ac nucleosome complex. ( C ) Zoom-in of the BD1 acetyl-lysine binding pocket highlighting residues Y97 and N140 positioned near H4K12ac. ( D ) Zoom-in of the BD2 acetyl-lysine binding pocket highlighting residues Y390 and N433 positioned near H4K12ac. This view also shows the relevant placement of the C-terminal BRD4-S region including the BID, ET, and phosphoregulatory sites, in the model.

Techniques: Binding Assay

Bromodomain binding pocket mutations in the BRD4-S disrupt breast cancer growth and migration phenotypes. ( A ) Cell growth assay following BRD4-S depletion and complementation with the indicated 3xFLAG-BRD4-S constructs (mean ± SEM; n≥5 independent experiments). Statistical analysis as described in Methods. ( B ) Transwell migration assay following BRD4-S depletion and complementation with the indicated 3xFLAG-BRD4-S constructs (mean ± SEM; n ≥ 3 independent experiments). Statistical significance was calculated by two-tailed t-tests (***p<0.0005, ****p<0.0001). ( C ) Western blots of endogenous BRD4-S depletion and rescue by 3xFLAG-tagged BRD4-S (1-719) constructs in doxycycline-treated MDA-MB-231 cells. Blots were probed with an antibody raised for the N-terminus of BRD4 (BRD4-S; for endogenous ∼100 kDa), M2 FLAG (FLAG), and β-actin (Actin) ( D ) Graphical illustration of putative model for BRD4-S chromatin engagement and regulation of cancer cell growth and migration (BioRender, https://BioRender.com/7dfa8yg ). This model shows multiple possible binding modes of BRD4-S on poly-acetylated nucleosomes that would be difficult to distinguish or determine in biochemical and structural studies, yet could all contribute to recruitment of regulatory complexes and transcriptional control of nearby genes including those for cancer growth and migration.

Journal: bioRxiv

Article Title: Tandem bromodomains of BRD4 cooperatively read poly-acetylated nucleosomes to enhance chromatin engagement and regulate breast cancer phenotypes

doi: 10.64898/2026.04.22.719657

Figure Lengend Snippet: Bromodomain binding pocket mutations in the BRD4-S disrupt breast cancer growth and migration phenotypes. ( A ) Cell growth assay following BRD4-S depletion and complementation with the indicated 3xFLAG-BRD4-S constructs (mean ± SEM; n≥5 independent experiments). Statistical analysis as described in Methods. ( B ) Transwell migration assay following BRD4-S depletion and complementation with the indicated 3xFLAG-BRD4-S constructs (mean ± SEM; n ≥ 3 independent experiments). Statistical significance was calculated by two-tailed t-tests (***p<0.0005, ****p<0.0001). ( C ) Western blots of endogenous BRD4-S depletion and rescue by 3xFLAG-tagged BRD4-S (1-719) constructs in doxycycline-treated MDA-MB-231 cells. Blots were probed with an antibody raised for the N-terminus of BRD4 (BRD4-S; for endogenous ∼100 kDa), M2 FLAG (FLAG), and β-actin (Actin) ( D ) Graphical illustration of putative model for BRD4-S chromatin engagement and regulation of cancer cell growth and migration (BioRender, https://BioRender.com/7dfa8yg ). This model shows multiple possible binding modes of BRD4-S on poly-acetylated nucleosomes that would be difficult to distinguish or determine in biochemical and structural studies, yet could all contribute to recruitment of regulatory complexes and transcriptional control of nearby genes including those for cancer growth and migration.

Techniques: Binding Assay, Migration, Growth Assay, Construct, Transwell Migration Assay, Two Tailed Test, Western Blot, Control

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recombinant mononucleosomes (EpiCypher)

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