Modification:Article Title: Plantaricin NC8 αβ rapidly and efficiently inhibits flaviviruses and SARS-CoV-2 by disrupting their envelopes
Article Snippet: .. Monkey ( Cercopithecus aethiops ) epithelial kidney cells (Vero E6, ATCC, CRL-1586), dog ( Canis familiaris ) epithelial kidney cells (MDCK, ATCC, CCL-34), and human lung epithelial cells (A549, ATCC CCL-185) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 1 g/L glucose (Gibco), supplemented with 10% heat-inactivated fetal bovine serum (HI‒FBS, Gibco) and 100 U/mL penicillin-streptomycin (PEST, Gibco) at 37°C in 95% air and 5% CO 2 . .. Jurkat T-cells cells (ATCC, TIB-152) were maintained in RPMI 1640 medium (Fisher scientific, Austria) with 1.5 mM L -glutamine (Invitrogen, USA) and supplemented with 10% FBS.
Article Title: Plantaricin NC8 αβ rapidly and efficiently inhibits flaviviruses and SARS-CoV-2 by disrupting their envelopes.
Article Snippet: .. Monkey (Cercopithecus aethiops) epithelial kidney cells (Vero E6, ATCC, CRL-1586), dog (Canis familiaris) epithelial kidney cells (MDCK, ATCC, CCL-34), and human lung epithelial cells (A549, ATCC CCL-185) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 1 g/L glucose (Gibco), supplemented with 10% heat-inactivated fetal bovine serum (HI–FBS, Gibco) and 100 U/mL penicillin-streptomycin (PEST, Gibco) at 37 ̊C in 95% air and 5% CO2. .. Jurkat T-cells cells (ATCC, TIB-152) were maintained in RPMI 1640 medium (Fisher scientific, Austria) with 1.5 mM L-glutamine (Invitrogen, USA) and supplemented with 10% FBS.
Cell Culture:Article Title: Furopyridine Derivatives as Potent Inhibitors of the Wild Type, L858R/T790M, and L858R/T790M/C797S EGFR.
Article Snippet: The treatment of patients with nonsmall cell lung cancer (NSCLC) using epidermal growth factor receptor (EGFR) inhibitors is complicated by drug-sensitive activating L858R/ T790M and L858R/T790M/C797S mutations.. To overcome drug resistance, a series of furopyridine (PD) compounds were virtually screened to identify potent EGFR inhibitors using molecular docking and molecular dynamics (MD) simulations based on the solvated interaction energy (SIE) method.. Several PD compounds identified from virtual screening demonstrated the potential to suppress both wild-type and mutant forms of EGFR, with IC50 values in the nanomolar range.
Article Title: Improved imaging and preservation of lysosome dynamics using silver nanoparticle-enhanced fluorescence
Article Snippet: .. Cell culture, plasmids, and transfection The male-mouse macrophage-like RAW 264.7 cell line was obtained from ATCC (TIB-71, VA) and cultured in DMEM with 5% heat inactivated fetal bovine serum (FBS), reseeded every 2–3 d. The Cos7 cell line is a kidney-fibroblast-like cell derived from a male-green monkey and was obtained from ATCC (CRL-1651) and were cultured in DMEM with 10% FBS. .. Both cell types were plated at 30–50% confluency and used within 24–48 h. Plasmids were obtained from Addgene as follows: plasmid #34831 encoding LAMP1-GFP was a gift from Esteban Dell’ Angelica, plasmid #73080 encoding galectin3-GFP was a gift from Tamotsu Yoshimori, and plasmid #22418 encoding mCherry-GFP-LC3b was provided by Jayantha Debnath and previously characterized (Falcón-Pérez et al., 2005; N’Diaye et al., 2009; Maejima et al., 2013).
Article Title: Furopyridine Derivatives as Potent Inhibitors of the Wild Type, L858R/T790M, and L858R/T790M/C797S EGFR
Article Snippet: Poly(glu-tyr) peptide (P61–58) was purchased from SignalChem Biotech (Canada). .. The lung carcinoma A549 (ATCC CCL-185), H1975 (ATCC CRL-5908), and monkey ( Cercopithecus aethiopsis ) kidney Vero cell line (ATCC CCL-81) cell lines were purchased from the American Type Cell Culture Collection (ATCC, Manassas, VA). .. Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute (RPMI), fetal bovine serum (FBS), penicillin–streptomycin (Pen–Strep), and trypsin were purchased from Life Technologies (CA).
Transfection:Article Title: Improved imaging and preservation of lysosome dynamics using silver nanoparticle-enhanced fluorescence
Article Snippet: .. Cell culture, plasmids, and transfection The male-mouse macrophage-like RAW 264.7 cell line was obtained from ATCC (TIB-71, VA) and cultured in DMEM with 5% heat inactivated fetal bovine serum (FBS), reseeded every 2–3 d. The Cos7 cell line is a kidney-fibroblast-like cell derived from a male-green monkey and was obtained from ATCC (CRL-1651) and were cultured in DMEM with 10% FBS. .. Both cell types were plated at 30–50% confluency and used within 24–48 h. Plasmids were obtained from Addgene as follows: plasmid #34831 encoding LAMP1-GFP was a gift from Esteban Dell’ Angelica, plasmid #73080 encoding galectin3-GFP was a gift from Tamotsu Yoshimori, and plasmid #22418 encoding mCherry-GFP-LC3b was provided by Jayantha Debnath and previously characterized (Falcón-Pérez et al., 2005; N’Diaye et al., 2009; Maejima et al., 2013).
Derivative Assay:Article Title: Improved imaging and preservation of lysosome dynamics using silver nanoparticle-enhanced fluorescence
Article Snippet: .. Cell culture, plasmids, and transfection The male-mouse macrophage-like RAW 264.7 cell line was obtained from ATCC (TIB-71, VA) and cultured in DMEM with 5% heat inactivated fetal bovine serum (FBS), reseeded every 2–3 d. The Cos7 cell line is a kidney-fibroblast-like cell derived from a male-green monkey and was obtained from ATCC (CRL-1651) and were cultured in DMEM with 10% FBS. .. Both cell types were plated at 30–50% confluency and used within 24–48 h. Plasmids were obtained from Addgene as follows: plasmid #34831 encoding LAMP1-GFP was a gift from Esteban Dell’ Angelica, plasmid #73080 encoding galectin3-GFP was a gift from Tamotsu Yoshimori, and plasmid #22418 encoding mCherry-GFP-LC3b was provided by Jayantha Debnath and previously characterized (Falcón-Pérez et al., 2005; N’Diaye et al., 2009; Maejima et al., 2013).
Incubation:Article Title: Exploring Synthetic Dihydrobenzofuran and Benzofuran Neolignans as Antiprotozoal Agents against Trypanosoma cruzi
Article Snippet: .. Monkey ( Macaca mulata ) epithelial kidney cell line LLC-MK2 (ATCC ® CCL-7TM) was cultivated in RPMI 1640 medium (Roswell Park Memorial Institute; Gibco-Life Technology, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (SFB) and antibiotics (penicillin: 10,000 UI/mL and streptomycin: 10,000 mg/mL) and incubated at 37 °C in a 5% CO 2 humidified incubator (Sanyo, Osaka, Japan) [ ]. .. The total spleen cell suspension was prepared using organs obtained from Swiss mice and macerated with RPMI 1640 medium (Gibco-Life Technology, Grand Island, NY, USA) in a Petri dish using a nylon sieve and a rubber stopper.
other:Article Title: Discovery of JAK2/3 Inhibitors from Quinoxalinone-Containing Compounds
Article Snippet: Human erythroleukemia TF1 (ATCC CRL-2003) and HEL 92.1.7 (ATCC TIB-180) cell lines, human liver cancer HepG2 cell line (ATCC HB-8065) and monkey ( Cercopithecus aethiops ) kidney Vero cell line (ATCC CCL-81) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).
|