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rpmi 1640 medium atcc modification  (ATCC)


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    ATCC rpmi 1640 medium atcc modification
    Rpmi 1640 Medium Atcc Modification, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 19666 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modifications/RPMI-1640+Medium/us12649798-951-22-25
    Average 99 stars, based on 19666 article reviews
    rpmi 1640 medium atcc modification - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    In Vitro:

    Article Title: Biological Properties of Extracts from Byrsonima Species in Microemulsions
    Article Snippet: The Byrsonima genus, Malpighiaceae, represents a rich source of phenolic compounds with proven pharmacological properties (antiulcerogenic, antimicrobial, antimutagenic, and antioxidant activities).. In this context, the aim of the present study was to identify chemical compounds employing UPLC-PDA-ESI-QTOF-MS/MS and FIA-ESI-IT-MS and evaluate the antimicrobial activity and toxicogenetic effects of hydroethanolic extracts obtained from B. coccolobifolia Kunth and B. ligustrifolia Mart. incorporated and not incorporated in microemulsions.. The former presents grape seed oil and cholesterol at a 5:1 ratio, as an oily phase (10%) (microemulsion 1) and the other, only cholesterol (microemulsion 2).

    Incubation:

    Article Title: Antimicrobial Activity of Some Steroidal Hydrazones
    Article Snippet: .. The potential of compounds to inhibit biofilm formation was investigated as previously described, with some modifications [ ] C. albicans ATCC 10231 was incubated in 96-well microtiter plates with an adhesive bottom (Sarstedt, Germany), with MIC and sub-MIC concentrations of tested compounds/referent drug in YPD medium at 37 °C for 24 h. Afterwards, wells were washed thrice with sterile PBS (Phosphate buffered saline, pH 7.4) and biofilms were fixed with methanol for 20 min. Then, methanol was removed and stained with 0.1% crystal violet (Bio-Merieux, France) for 30 min. .. The plate was slowly washed, air dried and 96% ethanol (Zorka, Serbia) was added to dissolve bounded crystal violet.

    Article Title: Antimicrobial Activity of Some Steroidal Hydrazones.
    Article Snippet: .. Inhibition of Biofilm Formation The potential of compounds to inhibit biofilm formation was investigated as previously described, with some modifications [36] C. albicans ATCC 10231 was incubated in 96-well microtiter plates with an adhesive bottom (Sarstedt, Germany), with MIC and sub-MIC concentrations of tested compounds/referent drug in YPD medium at 37 ◦C for 24 h. Afterwards, wells were washed thrice with sterile PBS (Phosphate buffered saline, pH 7.4) and biofilms were fixed with methanol for 20 min. Then, methanol was removed and stained with 0.1% crystal violet (Bio-Merieux, France) for 30 min. .. The plate was slowly washed, air dried and 96% ethanol (Zorka, Serbia) was added to dissolve bounded crystal violet.

    Adhesive:

    Article Title: Antimicrobial Activity of Some Steroidal Hydrazones
    Article Snippet: .. The potential of compounds to inhibit biofilm formation was investigated as previously described, with some modifications [ ] C. albicans ATCC 10231 was incubated in 96-well microtiter plates with an adhesive bottom (Sarstedt, Germany), with MIC and sub-MIC concentrations of tested compounds/referent drug in YPD medium at 37 °C for 24 h. Afterwards, wells were washed thrice with sterile PBS (Phosphate buffered saline, pH 7.4) and biofilms were fixed with methanol for 20 min. Then, methanol was removed and stained with 0.1% crystal violet (Bio-Merieux, France) for 30 min. .. The plate was slowly washed, air dried and 96% ethanol (Zorka, Serbia) was added to dissolve bounded crystal violet.

    Article Title: Antimicrobial Activity of Some Steroidal Hydrazones.
    Article Snippet: .. Inhibition of Biofilm Formation The potential of compounds to inhibit biofilm formation was investigated as previously described, with some modifications [36] C. albicans ATCC 10231 was incubated in 96-well microtiter plates with an adhesive bottom (Sarstedt, Germany), with MIC and sub-MIC concentrations of tested compounds/referent drug in YPD medium at 37 ◦C for 24 h. Afterwards, wells were washed thrice with sterile PBS (Phosphate buffered saline, pH 7.4) and biofilms were fixed with methanol for 20 min. Then, methanol was removed and stained with 0.1% crystal violet (Bio-Merieux, France) for 30 min. .. The plate was slowly washed, air dried and 96% ethanol (Zorka, Serbia) was added to dissolve bounded crystal violet.

    Sterility:

    Article Title: Antimicrobial Activity of Some Steroidal Hydrazones
    Article Snippet: .. The potential of compounds to inhibit biofilm formation was investigated as previously described, with some modifications [ ] C. albicans ATCC 10231 was incubated in 96-well microtiter plates with an adhesive bottom (Sarstedt, Germany), with MIC and sub-MIC concentrations of tested compounds/referent drug in YPD medium at 37 °C for 24 h. Afterwards, wells were washed thrice with sterile PBS (Phosphate buffered saline, pH 7.4) and biofilms were fixed with methanol for 20 min. Then, methanol was removed and stained with 0.1% crystal violet (Bio-Merieux, France) for 30 min. .. The plate was slowly washed, air dried and 96% ethanol (Zorka, Serbia) was added to dissolve bounded crystal violet.

    Article Title: Antimicrobial Activity of Some Steroidal Hydrazones.
    Article Snippet: .. Inhibition of Biofilm Formation The potential of compounds to inhibit biofilm formation was investigated as previously described, with some modifications [36] C. albicans ATCC 10231 was incubated in 96-well microtiter plates with an adhesive bottom (Sarstedt, Germany), with MIC and sub-MIC concentrations of tested compounds/referent drug in YPD medium at 37 ◦C for 24 h. Afterwards, wells were washed thrice with sterile PBS (Phosphate buffered saline, pH 7.4) and biofilms were fixed with methanol for 20 min. Then, methanol was removed and stained with 0.1% crystal violet (Bio-Merieux, France) for 30 min. .. The plate was slowly washed, air dried and 96% ethanol (Zorka, Serbia) was added to dissolve bounded crystal violet.

    Saline:

    Article Title: Antimicrobial Activity of Some Steroidal Hydrazones
    Article Snippet: .. The potential of compounds to inhibit biofilm formation was investigated as previously described, with some modifications [ ] C. albicans ATCC 10231 was incubated in 96-well microtiter plates with an adhesive bottom (Sarstedt, Germany), with MIC and sub-MIC concentrations of tested compounds/referent drug in YPD medium at 37 °C for 24 h. Afterwards, wells were washed thrice with sterile PBS (Phosphate buffered saline, pH 7.4) and biofilms were fixed with methanol for 20 min. Then, methanol was removed and stained with 0.1% crystal violet (Bio-Merieux, France) for 30 min. .. The plate was slowly washed, air dried and 96% ethanol (Zorka, Serbia) was added to dissolve bounded crystal violet.

    Article Title: Antimicrobial Activity of Some Steroidal Hydrazones.
    Article Snippet: .. Inhibition of Biofilm Formation The potential of compounds to inhibit biofilm formation was investigated as previously described, with some modifications [36] C. albicans ATCC 10231 was incubated in 96-well microtiter plates with an adhesive bottom (Sarstedt, Germany), with MIC and sub-MIC concentrations of tested compounds/referent drug in YPD medium at 37 ◦C for 24 h. Afterwards, wells were washed thrice with sterile PBS (Phosphate buffered saline, pH 7.4) and biofilms were fixed with methanol for 20 min. Then, methanol was removed and stained with 0.1% crystal violet (Bio-Merieux, France) for 30 min. .. The plate was slowly washed, air dried and 96% ethanol (Zorka, Serbia) was added to dissolve bounded crystal violet.

    Staining:

    Article Title: Antimicrobial Activity of Some Steroidal Hydrazones
    Article Snippet: .. The potential of compounds to inhibit biofilm formation was investigated as previously described, with some modifications [ ] C. albicans ATCC 10231 was incubated in 96-well microtiter plates with an adhesive bottom (Sarstedt, Germany), with MIC and sub-MIC concentrations of tested compounds/referent drug in YPD medium at 37 °C for 24 h. Afterwards, wells were washed thrice with sterile PBS (Phosphate buffered saline, pH 7.4) and biofilms were fixed with methanol for 20 min. Then, methanol was removed and stained with 0.1% crystal violet (Bio-Merieux, France) for 30 min. .. The plate was slowly washed, air dried and 96% ethanol (Zorka, Serbia) was added to dissolve bounded crystal violet.

    Article Title: Antimicrobial Activity of Some Steroidal Hydrazones.
    Article Snippet: .. Inhibition of Biofilm Formation The potential of compounds to inhibit biofilm formation was investigated as previously described, with some modifications [36] C. albicans ATCC 10231 was incubated in 96-well microtiter plates with an adhesive bottom (Sarstedt, Germany), with MIC and sub-MIC concentrations of tested compounds/referent drug in YPD medium at 37 ◦C for 24 h. Afterwards, wells were washed thrice with sterile PBS (Phosphate buffered saline, pH 7.4) and biofilms were fixed with methanol for 20 min. Then, methanol was removed and stained with 0.1% crystal violet (Bio-Merieux, France) for 30 min. .. The plate was slowly washed, air dried and 96% ethanol (Zorka, Serbia) was added to dissolve bounded crystal violet.

    Cell Culture:

    Article Title: Improvement of mechanical properties of collagen electrospun mats by halloysite nanotubes
    Article Snippet: .. The cell culture was prepared using the procedures described by the suppliers of the cell lines with minor modifications (Primary Dermal Fibroblasts; Normal, Human, Adult (ATCC® PCSTM 201-012TM)). ..

    Diagnostic Assay:

    Article Title: Survival of 21 Dogs after Post-exposure Prophylaxis using Intra-dermal Rabies Vaccine in the Pre-scapular Region and Emergency Use of Expired Equine Rabies Immunoglobulin for Local Wound Infiltration in Victims of a Rabid Dog Bite using One Health Approach
    Article Snippet: .. RFFIT for estimation of RVNA was performed at the KVAFSUCVA Rabies diagnostic laboratory, Veterinary College, KVAFSU, Hebbal, Bengaluru, using a WHO recommended procedure with minor modifications (Smith et al., 1996).10 BHK-21 cell line (ATCC CCL 10) and BHK-21 adapted CVS 11 strain of virus were used and the tests were performed in 96 well tissue culture plates. ..

    Virus:

    Article Title: Survival of 21 Dogs after Post-exposure Prophylaxis using Intra-dermal Rabies Vaccine in the Pre-scapular Region and Emergency Use of Expired Equine Rabies Immunoglobulin for Local Wound Infiltration in Victims of a Rabid Dog Bite using One Health Approach
    Article Snippet: .. RFFIT for estimation of RVNA was performed at the KVAFSUCVA Rabies diagnostic laboratory, Veterinary College, KVAFSU, Hebbal, Bengaluru, using a WHO recommended procedure with minor modifications (Smith et al., 1996).10 BHK-21 cell line (ATCC CCL 10) and BHK-21 adapted CVS 11 strain of virus were used and the tests were performed in 96 well tissue culture plates. ..

    Inhibition:

    Article Title: Antimicrobial Activity of Some Steroidal Hydrazones.
    Article Snippet: .. Inhibition of Biofilm Formation The potential of compounds to inhibit biofilm formation was investigated as previously described, with some modifications [36] C. albicans ATCC 10231 was incubated in 96-well microtiter plates with an adhesive bottom (Sarstedt, Germany), with MIC and sub-MIC concentrations of tested compounds/referent drug in YPD medium at 37 ◦C for 24 h. Afterwards, wells were washed thrice with sterile PBS (Phosphate buffered saline, pH 7.4) and biofilms were fixed with methanol for 20 min. Then, methanol was removed and stained with 0.1% crystal violet (Bio-Merieux, France) for 30 min. .. The plate was slowly washed, air dried and 96% ethanol (Zorka, Serbia) was added to dissolve bounded crystal violet.

    Modification:

    Article Title: Characterization of multidrug and heavy metal resistance of carbapenemases producing Klebsiella pneumoniae from poultry samples in Bangladesh.
    Article Snippet: .. The production of carbapenemase was determined using the modified Hodge test (MHT) according to the CLSI guidelines (Clinical and Laboratory Standards Institute (CLSI, 2016) and as described elsewhere (Okoche et al., 2015) with some modifications (not using Escherichia coli ATCC 25922 but using E. coli-DH5 isolate that is susceptible to carbapenem antibiotics). ..

    Article Title: Characterization of multidrug and heavy metal resistance of carbapenemases producing Klebsiella pneumoniae from poultry samples in Bangladesh
    Article Snippet: .. The production of carbapenemase was determined using the modified Hodge test (MHT) according to the CLSI guidelines (Clinical and Laboratory Standards Institute (CLSI, ) and as described elsewhere (Okoche et al., ) with some modifications (not using Escherichia coli ATCC 25922 but using E. coli ‐DH5 isolate that is susceptible to carbapenem antibiotics). ..



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